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Full-Text Articles in Life Sciences

Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu Aug 2023

Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu

IGC Proceedings (1993-2023)

In recent years, biological indicators are being used to estimate the continued capacity of a given soil to function (i.e., soil health). After all, biological processes are intimately linked with the maintenance of soil structure and fertility, being more sensitive to changes in the soil than conventional physicochemical parameters. Soil enzymes, as mediators and catalysts of vital soil functions, offer great potential as integrative indicators of soil health (Dick et al., 1996). The main aim of the current work was to study the potential of soil enzyme activities as biological (more precisely, biochemical) indicators of soil physicochemical …


Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger May 2023

Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger

IGC Proceedings (1993-2023)

Tropical grasses are the primary staple diet of most of the domesticated ruminants in the tropical and subtropical regions of the world. However, dry matter (DM) digestibility (DMD) and intake levels of these C4 grasses are considerably low, partly because of high lignin contents. Ferulic acid also impedes fibre digestion in such forages due to formation of cross linkages with digestible xylans. Certain esterase enzymes have been shown to cleave ferulic acid cross linkages in wheat bran but no studies have examined whether such enzymes can be used to increase the digestion of tropical grasses. Therefore, the objective of …


The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer Jan 2023

The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted to examine the effects of Allzyme® Spectrum supplementation to a reduced nutrient diet on the growth performance, energy and nutrient digestibility and utilization, bone quality, and jejunal digesta viscosity in broiler chickens. Each experiment used 300 one-day old Cobb by-product breeder chicks with 5 treatments, 10 replicates, and 6 birds per replicate cage in a randomized complete block design. The only differences between the two experiments were experiment 1 lasted for 21 days using a corn-soybean meal-based diet and experiment 2 lasted for 22 days using a wheat-soybean meal-based diet. The 5 treatments included a positive …


Effects Of Different Additives On Silage Quality Of Napiergrass, Y. K. Cheng, C. S. Chen, P. W. Peng Dec 2021

Effects Of Different Additives On Silage Quality Of Napiergrass, Y. K. Cheng, C. S. Chen, P. W. Peng

IGC Proceedings (1993-2023)

Objectives of this experiment were to determine the effects of different additives on silage quality of napiergrass (Pennisetum purpureum). The cultivar of TLG2 with high water soluble carbohydrates was used to making silages added with or without 5 and 10% corn meal or wheat bran, and then treated with or without enzyme. Crude protein, acid detergent fiber, neutral detergent fiber, water soluble carbohydrates and volatile fatty acid contents of silage were determined to evaluate silage quality. From the results, it showed that var.TLG2 could be making good silages without any additives but the dry matter was low. Adding …


Coccidia Vaccine Challenge And Exogenous Enzyme Supplementation In Broiler Chicken 1. Effect On Digesta Viscosity, Diet Energy Utilization, And Apparent Metabolizable Energy Value Of Wheat, Andrew E. Dunaway, Sunday A. Adedokun Feb 2021

Coccidia Vaccine Challenge And Exogenous Enzyme Supplementation In Broiler Chicken 1. Effect On Digesta Viscosity, Diet Energy Utilization, And Apparent Metabolizable Energy Value Of Wheat, Andrew E. Dunaway, Sunday A. Adedokun

Animal and Food Sciences Faculty Publications

This study examined the effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) to a corn-SBM (CS) and a wheat-CS-based (WCS) diet in birds challenged with coccidia vaccine (Coccivac B-52™). The WCS-based diet was produced by replacing 30% of the energy-yielding portions of the CS-based diet with wheat. On day 14, 448 (n = 7) Cobb by-product breeder male broilers were assigned to a 2 (diet types) × 2 (with or without enzyme supplementation) × 2 (0 or 20 × coccidia vaccine challenge; CVC) factorial arrangement of treatments in a completely randomized design for the determination of the apparent …


Effects Of Different Enzyme Combination And Dissociation‐Time On The Protoplast Isolation Of Alfalfa, Aiping Chen, Yupeng Luo, Run Yan, Bo Zhang Jun 2020

Effects Of Different Enzyme Combination And Dissociation‐Time On The Protoplast Isolation Of Alfalfa, Aiping Chen, Yupeng Luo, Run Yan, Bo Zhang

IGC Proceedings (1993-2023)

No abstract provided.


Amino Acid Racemase Enzyme Assays, Atanas D. Radkov, Luke A. Moe May 2014

Amino Acid Racemase Enzyme Assays, Atanas D. Radkov, Luke A. Moe

Plant and Soil Sciences Faculty Publications

Amino acid racemases are enzymes that invert the α-carbon stereochemistry of amino acids (AAs), interconverting amino acids between their L- and D-enantiomers in a reversible reaction. In bacteria, they are known to have catabolic physiological functions but are also involved in the synthesis of many D-AAs, including D-glutamate and D-alanine, which are necessary components of the peptidoglycan layer of the bacterial cell wall. As such, amino acid racemases represent significant targets for the development of bactericidal compounds. Amino acid racemases are also regarded by the biotechnological industry as important catalysts for the production of economically relevant D-AAs. Here, we provide …


B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus Apr 2014

B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus

Plant and Soil Sciences Faculty Patents

Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the …