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Full-Text Articles in Life Sciences

Microbiome Of The Cool Season Forage Grass Timothy (Phleum Pratense L.) And Its Potential Role In Stress Tolerance, Dina Saleh, Suha Jabaji, Philippe Seguin Feb 2022

Microbiome Of The Cool Season Forage Grass Timothy (Phleum Pratense L.) And Its Potential Role In Stress Tolerance, Dina Saleh, Suha Jabaji, Philippe Seguin

IGC Proceedings (1997-2023)

The functional attributes of the microbiome associated with timothy for growth promotion properties, antimicrobial and biosurfactant capacities were characterized. A total of 254 culturable bacteria were identified by 16S rRNA sequencing and grouped into 16 taxa that shared high homology of 98–99% with other known sequences. The majority of bacterial isolates exhibited multifunctional growth promotion attributes and plant stress improvement. The selection of competent and compatible strains for application in forage production is dependent on the recognition of root exudates and motility towards the roots, attachment to the root surface, formation of biofilm, penetration, and colonization of internal tissues. We …


Insights Into Triterpene Metabolism In Model Monocotyledonous And Oilseed Plants Genetically Engineered With Genes From Botryococcus Braunii, Chase F. Kempinski Jan 2016

Insights Into Triterpene Metabolism In Model Monocotyledonous And Oilseed Plants Genetically Engineered With Genes From Botryococcus Braunii, Chase F. Kempinski

Theses and Dissertations--Plant and Soil Sciences

Isoprenoids are one of the most diverse classes of natural products and are all derived from universal five carbon, prenyl precursors. Squalene and botryococcene are linear, hydrocarbon triterpenes (thirty carbon compounds with six prenyl units) that have industrial and medicinal values. Squalene is produced by all eukaryotes as it is the first committed precursor to sterols, while botryococcene is uniquely produced by the green algae, Botryococcus braunii (race B). Natural sources for these compounds exist, but there is a desire for more renewable production platforms. The model plant Arabidopsis thaliana was engineered to accumulate botryococcene and squalene in its oil …


Probing The Plant Cell Wall With Herbicides: A Chemical Genetics Approach, Chad B. Brabham Jan 2016

Probing The Plant Cell Wall With Herbicides: A Chemical Genetics Approach, Chad B. Brabham

Theses and Dissertations--Plant and Soil Sciences

The primary cell wall is a highly organized multi-layered matrix of polysaccharides (cellulose, hemi-cellulose, and pectin). The ability of the rigid cell wall to sufficiently loosen to allow growth is a complex process that differs considerably between grasses monocots and dicots. Cellulose is the major structural component required for anisotropic cell expansion and is synthesized by CELLULOSE SYNTHASE A (CesA) proteins. Here, our objectives were two-fold: 1) dissect cell walls and cellulose biosynthesis in dicots and grasses using chemical biology and reverse genetic approaches 2) characterize and classify the inhibitory mechanisms of cellulose biosynthesis inhibitors (CBIs). A reverse genetics TILLING …