Life Sciences Commons^™

Principals' And School Food Service Workers' Perceptions Of The Implementation Of The Community Eligibility Provision, Shirlena M. Moore

Theses and Dissertations--Nutrition and Food Systems

Over the years, participation in the School Breakfast Program (SBP) has increased, on both state and national levels. The Community Eligibility Provision (CEP) offers free breakfast to all students regardless of income. The purpose of this study was to examine the perception of principals and school food service workers regarding the benefits, barriers, attitudes and beliefs about the implementation of the CEP. A survey was sent to principals and school food service workers. The majority of the participants were satisfied overall with the CEP and the nutritional quality of foods served for breakfast. The majority of the participants did not …

Endosymbiotic Bacteria In The Bed Bug, Cimex Lectularius L. (Hemiptera: Cimicidae), Mark H. Goodman

Theses and Dissertations--Entomology

The return of bed bugs to prominence as an urban pest has precipitated an emphatic research response from the scientific community in an effort to better understand their biology and management. However, not all aspects of bed bug biology have received equal attention. The role of symbiotic bacteria in the normal function and biology of insects is often underappreciated, and this is true of bed bugs. Bed bugs have two main endosymbiotic bacteria, a Wolbachia species and a poorly-characterized gamma-proteobacterium referred to as BLS. However, their interactions with the host are poorly understood. I explored various aspects of these symbionts …

The Influence Of Time On Food Intake Patterns: Age, Period, Cohort Differences, Karina L. Christopher

Theses and Dissertations--Gerontology

The impact of diet and exercise on overall health and chronic disease risk has been well examined. Multiple studies show that Americans eat more now than they did fifty years ago. What isn’t known is how much of an impact time has on food intake patterns of individuals in terms of different age groups, historic periods of structural influences, and birth cohorts.

In order to identify the impact of time on food intake this study examined time from multiple perspectives. The first aim of this study was to determine food intake patterns among age groups across five time periods using …

The Role Of Astrocytic Calcineurin Activation And Downstream Signaling In Neurodegenerative Diseases, Melanie M. Pleiss

Theses and Dissertations--Pharmacology and Nutritional Sciences

Calcineurin (CN) is a calcium (Ca²⁺)-sensitive serine/threonine protein phosphatase that plays a significant role in several cell signaling pathways, and has been implicated in many neurodegenerative diseases including Alzheimer’s disease (AD) and vascular cognitive impairment and dementia (VCID). Although normally found in neurons, CN also appears at high levels in activated astrocytes under conditions of injury and disease. To elucidate the role of astrocytic calcineurin signaling in neurodegenerative diseases, our lab has used primary rat astrocytes, transgenic and diet-induced mouse models of dementia, and human tissue biospecimens from confirmed AD and VCID cases.

To better understand mechanisms for …

Deep Sleep, Cognition, Body Weight, Body Temperature, And Behavioral Distress Responses To New Onset Psychosocial Stressors Are Blunted With Age In Male F344 Rats, Kendra E. Hargis

Theses and Dissertations--Pharmacology and Nutritional Sciences

Complaints associated with aging, including cognitive deficits and sleep loss, are highly prevalent and negatively impact quality of life. Further, with increased age, humans are also more likely to experience new-onset psychosocial stressors, such as divorce, loss of a spouse, and social isolation. Stress has detrimental consequences that in many ways parallel the effects of aging on sleep and cognition. The long-standing stress/ glucocorticoid hypotheses of brain aging posit that stress exposure exacerbates aging symptoms, and extensive prior studies have shown that early life stress exposure does worsen phenotypic aging symptoms. However, despite its prevalence in aged humans, little basic …

Fatty Acid Profile In Ruminal Content And Blood Plasma Of Finishing Beef Cattle, Supplemented With Different Sources Of Fat, Amanda Pesqueira

Theses and Dissertations--Animal and Food Sciences

Eight steers were used in a replicated 4 x 4 Latin square design experiment to study the rumen metabolism of polyunsaturated fatty acids. The animals were fed a concentrate diet based on cracked corn grain, corn silage and hay fescue (K31). The dietary fat treatments were a negative control, tallow added at 60g/day, fish oil at 60g/day and heterotrophically grown microalgae high in DHA at 100g/day. Treatments were incorporated immediately prior to feeding each day and dosed through a ruminal fistula mixed with 0.45kg of the diet. Animals were fed at 1.75 x NEm once daily during adaptation then switched …

Functional Roles For Post-Translational Modifications Of T-Snares In Platelets, Jinchao Zhang

Theses and Dissertations--Molecular and Cellular Biochemistry

Platelets affect vascular integrity by secreting a host of molecules that promote hemostasis and its sequela. Given its importance, it is critical to understand how platelet exocytosis is controlled. Post-translational modifications, such as phosphorylation and acylation, have been shown to affect signaling pathways and platelet function. In this dissertation, I focus on how these modifications affect the t-SNARE proteins, SNAP-23 and syntaxin-11, which are both required for platelet secretion. SNAP-23 is regulated by phosphorylation. Using a proteoliposome fusion assay, I demonstrate that purified IκB Kinase (IKK) phosphorylated SNAP-23, which increased the initial rates of SNARE-mediated liposome fusion. SNAP-23 mutants …

Adaptability In A Bhutanese Refugee Community: Navigating Integration And The Impacts On Nutritional Health After U.S. Resettlement, Chris Grosh

Theses and Dissertations--Anthropology

Increasing rates of overweight, obesity, and related metabolic diseases documented among refugee communities across the United States necessitate greater attention to how processes of integration impact refugee health. These nutritional health trends (e.g., increasing rates of obesity) suggest potential disconnects between refugees' past environments and their conditions after re-settlement, which may contribute to adverse changes in energy balance (diet and exercise). While Bhutanese refugees were among the largest refugee groups entering the US during the five years leading up to this research, very few studies have examined how they have responded to integration and the impact of this transition on …

Comparison Of Quick Methods For Determining Body Composition In Female Collegiate Athletes And Obese Females, Mandee E. Martin

Theses and Dissertations--Nutrition and Food Systems

The Body Mass Index (BMI) is a tool used broadly by public health agencies to assess weight in populations. However, when differentiating between fat mass and fat free mass the formula (BMI = weight in kilograms/height in meters2) is not applicable. Research suggests that evaluating body fat percentage and adipose tissue deposition may provide a nuanced indication of overall health, making it more accurate on an individual basis. This study evaluated four methods (Body Mass Index, waist circumference, A Body Shape Index, and Waist to Stature Index) that assess body composition and their ability to predict body fat percentage in …

Promoting Healthy Eating And Physical Activity: A Qualitative Examination Of Community-Based Obesity Interventions In Rural Kentucky, Brooke F. Butterworth

Theses and Dissertations--Nutrition and Food Systems

Rural Americans are medically underserved groups and are at greater risk of becoming obese than urban Americans. The purpose of this qualitative study was to determine the perceived causes of obesity in six counties of rural Kentucky and to determine how to customize strategies to reduce obesity in these areas. University of Kentucky Cooperative Extension Service Agents formed coalitions in each of the six counties to assess their communities’ needs and assets to inform plans for implementing evidence-based obesity interventions. Between February and August of 2015, the coalitions were convened for a total of 11 meetings. Each of the coalition …

Associations Between Consumption Of Fruits And Vegetables Among Older Consumers And Farmers Market Shopping, Shu-Feng Tsao

Theses and Dissertations--Nutrition and Food Systems

The number of farmers’ markets in the United States has increased over 300% in past two decades. Many studies have also shown a positive association between an increased access to farmers’ markets and consumption of fruits and vegetables. However, few studies have explored relationships between older consumers aged 55+ whose fruit and vegetable consumption and their attendance at farmers’ markets. In Taiwan, no previous studies regarding farmers’ markets had been conducted from nutritional perspectives. The aims of this study were to determine general characteristics of farmers’ markets shoppers and their perceptions regarding the markets in Lexington, Kentucky and Taipei City, …

Effects Of Mountaintop Removal Mining On Population Dynamics Of Stream Salamanders, Sara B. Freytag

Theses and Dissertations--Forestry and Natural Resources

Mountaintop removal mining (MTR) is a notorious stressor of stream ecosystems in the Central Appalachians. Valley fills (VF) lead to reduced occupancy, abundance, and species richness of stream salamanders. Multiple factors may be responsible for these reductions, but specifically habitat fragmentation and degradation may reduce colonization rates and increase local extinction rates. From 2013-2015, repeated counts of salamanders were conducted in stream reaches impacted by MTR/VF and compared to counts in reference reaches to answer the question: do stream salamander population dynamics differ between stream reaches impacted by MTR/VF and reference stream reaches? I also investigated dynamics of stream habitat …

What’S In A Gene: Undergraduates’ Ideas And Misconceptions About Gene Function, Justin M. Levaughn

Theses and Dissertations--Science, Technology, Engineering, and Mathematics (STEM) Education

The purpose of this study was to field test a two-tiered instrument including multiple-choice and short answer tasks to assess college students’ ideas and level of understanding in genetics. The instrument was constructed from previously tested assessment tasks and findings from the current research literature. Ninety-seven freshmen enrolled in a biology lab course were surveyed. Test validity and reliability were measured using Chronbach coefficients. Multiple-choice and short answer responses were analyzed using descriptive statistics to identify frequencies of answer selections. Written responses were independently evaluated using a five-point scoring rubric by three researchers to identify common misconceptions revealed in students’ …

Effects Of Epichloë Coenophiala−Tall Fescue Symbiosis On Plant-Microbe-Soil Interactions In A Temperate Pasture, Lindsey C. Slaughter

Theses and Dissertations--Plant and Soil Sciences

Plants interact in myriad ways with microorganisms to influence ecosystem processes such as nutrient cycling, which can regulate ecosystem response to global change. One important plant-microbe symbiosis occurs between cool-season grasses and asexual fungal Epichloë endophytes, such as tall fescue (Schedonorus arundinaceus) and Epichloë coenophiala. Because the common toxic strain of the endophyte (CTE) harms grazing livestock, non-livestock toxic endophyte (NTE) strains have been developed and are increasingly deployed in pastures. Little is known about how these symbioses impact other plant-microbe interactions and microbe-mediated soil processes in grassland ecosystems. I conducted three studies to determine how E. …

Investigations In Cryptic Species: Considerations And Applications For Estimating Detection, Occupancy, And Abundance Of Semi-Aquatic Snakes, Christian Robert Oldham

Theses and Dissertations--Forestry and Natural Resources

Snake species are notoriously difficult to study in the field due to their cryptic natural-histories and secretive behaviors. Difficulties associated with detection present challenges estimating parameters including occupancy and abundance, as well as responses to habitat degradation. Our objectives were to use Passive Integrated Transponder (PIT) telemetry to enhance detection of Queensnakes (Regina septemvittata) as compared to traditional capture-mark-recapture (CMR) survey techniques and to examine occupancy and abundance of Queensnakes and Northern Watersnakes (Nerodia sipedon) in streams of differing levels of anthropogenic impact within Central Kentucky. During 2013, we captured Queensnakes and implanted them with PIT …

Effects Of Feeding A Yeast-Based Supplement Containing Docosahexaenoic Acid (Dha) From A Heterotrophically Grown Microalgae, Vitamin E, And Selenium On Stallion Sperm Motion Characteristics, Lauren D. Goedde

Theses and Dissertations--Veterinary Science

The use of cooled and frozen stallion semen has become quite popular. However, there are some stallions that have sperm that are quite susceptible to cold shock. Thus, there is a need for techniques that will alter sperm so that they can withstand the stresses of cooling and freezing and thus improve pregnancy rates achieved with cooled or frozen spermatozoa. Studies have shown that a diet high in omega-3 (n-3) fatty acids can improve the motility of cooled and frozen/thawed sperm. Many of the omega-3 fatty acid products for stallions have low levels of docosahexaenoic acid (DHA) and are based …

Variance Of The Amyloid Beta Peptide As A Metric For The Diagnosis Of Alzheimer's Disease, Christina Beckett

Theses and Dissertations--Medical Sciences

Alzheimer’s disease (AD) is the most prevalent neurodegenerative disorder associated with aging. AD is by far the best understood and most studied neurodegenerative disease. Substantial advances have been made over the last decade, however it is debatable how much closer we are to a clinically useful therapy. A long standing goal in the AD field has been to improve the accuracy of early detection, with the assumption that the ability to intervene earlier in the disease process will lead to a better clinical outcome. Major facets of this effort have been the continued development and improvement of AD biomarkers, with …

Elucidating Proteasome Catalytic Subunit Composition And Its Role In Proteasome Inhibitor Resistance, Kimberly C. Carmony

Theses and Dissertations--Pharmacy

Proteasome inhibitors bortezomib and carfilzomib are FDA-approved anticancer agents that have contributed to significant improvements in treatment outcomes. However, the eventual onset of acquired resistance continues to limit their clinical utility, yet a clear consensus regarding the underlying mechanisms has not been reached.

Bortezomib and carfilzomib are known to target both the constitutive proteasome and the immunoproteasome, two conventional proteasome subtypes comprising distinctive sets of catalytic subunits. While it has become increasingly evident that additional, ‘intermediate’ proteasome subtypes, which harbor non-standard mixtures of constitutive proteasome and immunoproteasome catalytic subunits, represent a considerable proportion of the proteasome population in many cell …

Post Weaning Supplementation Of April-Born Polypay And White Dorper Lambs Grazing Alfalfa/Orchardgrass Pasture, Lauren N. Wood

Theses and Dissertations--Animal and Food Sciences

The effect of post-weaning supplementation of April-born Polypay and White Dorper lambs grazing alfalfa/orchardgrass pasture during two grazing seasons from June to September was studied. One hundred seventy Polypay and 133 White Dorper lambs were randomly allotted to supplemented (2% BW daily) and unsupplemented groups. It was discovered that Polypay lambs weighed more than White Dorpers at wearning, when the grazing season began, and at the end of the 80 (Year 1) and 85 (Year 2) grazing season (P < 0.01). Polypay lambs gained faster (P < 0.01) than White Dorpers. Supplemented lambs gained faster (P < 0.01) than unsupplemented and Polypays had a greater response to supplementation (P < 0.01) than White Dorpers. Polypays had higher (P < 0.01) fecal egg counts, an indicator of Haemonchus contortus infestation. Supplementation did not have any consistent effect on reducing Haemonchus contortus infestation. Differences in forage characteristics of Polypay and White Dorper lambs did appear. Differences in alfalfa and orchardgrass dry matter, neutral detergent fiber, acid detergent fiber, and crude protein availability were measured by subtracting enter from exit availabilities. The largest decrease of alfalfa components from exit to enter was found with unsupplemented Polypays. No consistent effect was found for orchardgrass components.

Acoustic Emission Of Lactococcus Lactis Ssp. Lactis C2 Infected With Three Bacteriophages C2, Sk1 And Ml3, Luxi Meng

Theses and Dissertations--Animal and Food Sciences

The objective of this research was to monitor the Acoustic Emission (AE) produced by Lactococcus lactis ssp. lactis C2 infected with three bacteriophages (c2, sk1 and ml3 at 90 min) using an acoustic emission-monitoring device which was designed at University of Kentucky in Lexington KY. The acoustic emission data was collected and then analyzed. These Acoustic Emission (AE) data suggested that bacteriophage ml3, sk1 and c2 can easily be distinguished by the differences in Absolute Energy (ABE), Centroid Frequency (CF) and Peak Frequency (PF) signals. The AE data suggested that bacteriophage sk1 and c2 caused greater stress on the lactis …

Ecology Of Two Reintroduced Black Bear Populations In The Central Appalachians, Sean Mccarthy Murphy

Theses and Dissertations--Animal and Food Sciences

Reintroduced populations are vulnerable to demographic and environmental stochasticity, deleterious genetic effects, and reduced population fitness, all of which can increase extinction probability. Population viability is principle to determining the status of reintroduced populations and for guiding management decisions. To attempt to reestablish black bear (Ursus americanus) populations in the central Appalachians, two reintroductions using small founder groups occurred during the 1990s in the Big South Fork area along the Kentucky-Tennessee border (BSF) and in the Jefferson National Forest along the Kentucky-Virginia border (KVP). My objectives were to estimate demographic and genetic parameters, and to evaluate long-term viability …

Effects Of Dietary Amino Acid Supplementation On Measures Of Whole-Body And Muscle Protein Metabolism In Aged Horses, Christine M. Latham

Theses and Dissertations--Animal and Food Sciences

Sarcopenia is a condition that is most common in aged animals, and is characterized by the loss of skeletal muscle mass and integrity, and can lead to physical disability and poor quality of life. Since skeletal muscle protein synthesis can be limited by the availability of amino acids, supplementation of limiting amino acids to ameliorate the progression of sarcopenia has become a topic of interest in companion animal research. Although there is some data to support the idea that amino acid supplementation improves maintenance of muscle mass in aged horses, the cellular mechanisms behind that improvement have yet to be …

Towards Determination Of The Threonine Requirement Of Yearling Horses Fed Varying Dietary Compositions Using The Indicator Amino Acid Oxidation Method, Kelsey M. Smith

Theses and Dissertations--Animal and Food Sciences

The amino acid requirements of growing horses are currently unknown, and studies suggest that threonine is a limiting amino acid in common horse diets. Thus, the objective of this study was to determine the threonine requirement of growing horses fed two different forage to concentrate ratios using the indicator amino acid oxidation method. The study consisted of a high concentrate phase (HC; 60% concentrate and 40% forage) and a high forage phase (HF; 25% concentrate and 75% forage). Within each phase, 6 female yearling Thoroughbred horses were randomly assigned each of 6 dietary treatments in a 6 x 6 Latin …

Development Of New Biological Nanopores And Their Application For Biosensing And Disease Detection, Shaoying Wang

Theses and Dissertations--Pharmacy

Nanopore technology has recently emerged as a new real-time single molecule sensing method. The current dominant technologies, such as mass spectrometry and immunoassay, for protein analysis is still slow and complex, which can’t meet the urgent need and fields of use. Development of a highly simple, portable and sensitive detection system for pathogen detection, disease diagnosis, and environmental monitoring is in great need. Membrane embedded Phi29 connector nanopore, the first protein nanopore coming from bacteriophage, was mainly focusing on DNA and RNA translocation in previous studies. Here, Phi29 connector nanopore was first time established for antibody detection by engineering Epithelial …

Probing The Functional Relevance Of Reactive Hippocampal Neurogenesis In A Model Of An Alcohol Use Disorder, Chelsea Rhea Geil Nickell

Theses and Dissertations--Pharmacy

Alcoholism, or alcohol use disorders (AUDs), represent a major public health concern both locally and globally. Critically, excessive alcohol consumption results in neurodegeneration in brain regions such as the hippocampus which is known for its role in learning and memory. Recovery of hippocampal volume loss has been observed after prolonged abstinence, but the mechanisms underlying this process are not well understood. Adult neurogenesis is thought to contribute to this recovery since after alcohol exposure a reactive increase in adult neurogenesis is observed. This reactive neurogenesis (the process by which brain insult results in a compensatory increase in neurogenesis) may represent …

Rna Nanotechnology For Next Generation Targeted Drug Delivery, Fengmei Pi

Theses and Dissertations--Pharmacy

The emerging field of RNA nanotechnology is developing into a promising platform for therapeutically application. Utilizing the state-of-art RNA nanotechnology, RNA nanoparticles can be designed and constructed with controllable shape, size for both RNA therapeutics and chemical drug delivery. The high homogeneity in particle size and ease for RNA therapeutic module conjugation, made it feasible to explore versatile RNA nanoparticle designs for preclinical studies.

One vital module for therapeutic RNA nanoparticle design is RNA aptamer, which can enable the RNA nanoparticles find its specific target for targeted drug delivery. A system of screening divalent RNA aptamers for cancer cell targeting …

Relationships Between Behavioral Measures And Productivity In Finishing Beef Cattle, Jasmyn Mimiko

Theses and Dissertations--Animal and Food Sciences

The relationship between measures of temperament, growth performance, and social hierarchy in finishing beef cattle were explored in two experiments. In experiment 1, high OCS (objective chute score) steers had periods of significantly higher ADG (P < 0.01), but OCS had no relationship with dominance ranking (P > 0.47). Conversely, slow exit velocity (EV) correlated with higher ranking (P ≤ 0.06), but EV had no relationship with performance (P > 0.37) in a competitive environment. Rank showed no relationship with performance (P > .58). In experiment 2, steers with fast EV had periods of decreased growth (P ≤ .06), intake (P ≤ .06), and gain:feed (G:F; P = 0.02). There were no interactions between …

Effect Of Maternal Diet On Select Fecal Bacteria In Mares And Their Foals, Morgan Brewer Pyles

Theses and Dissertations--Animal and Food Sciences

The process of microbial colonization in the gastrointestinal tract of foals and the factors influencing this process are not well understood. The objectives were to determine the effect of starch source in pelleted concentrates in the maternal diet on select fecal bacterial groups in mares and their foals and to evaluate changes in these bacterial groups over time. Thoroughbred foals (n=19) were from dams fed one of two concentrates: an oat-based (OB) or corn and wheat middling-based (CWB) pelleted concentrate. Fecal samples were collected from mares at 324 d of gestation, pre-foaling, 1 d, 14 d, and 28 d after …

Specialty Poultry Production: Impact Of Genotype, Feed Strategies, Alternative Feedstuffs, And Dietary Enzymes On The Growth Performance And Carcass Characteristics Of Heritage Breed Chickens, Tatijana Fisher

Theses and Dissertations--Animal and Food Sciences

There is a growing market for specialty poultry production using alternative genotypes and management systems. However, producers interested in specialty poultry production face several challenges. One challenge is that little published data exists regarding the growth and production parameters for alternative genotypes like slow-growing meat strains and heritage breeds. To address this challenge, research at the University of Kentucky examined the effect of feed strategies, alternative feedstuffs, and dietary enzymes on the growth and performance of heritage breeds of chicken used for either egg- or meat-production. The first trial documented the growth and nutrient intake of pullets from three heritage …

Relative Reactivity Of Protein And Lipid To Oxidants In Different Bi-Phasic Systems And Its Implication In Sausage Quality, Jiayi Yang

Theses and Dissertations--Animal and Food Sciences

This study investigated the progression of protein and lipid oxidation in different bi-phasic model systems: simple aqueous dispersions, liposome, and oil-in-water (O/W) emulsions. Varied concentrations of isolated pork myofibrillar protein (MFP) were mixed with free fatty acids (C18:1, C18:2, C18:3), lecithin, or vegetable oil to construct different lipid-protein dispersion systems, then subjected to hydroxyl radical stress at 4 °C. Comparison of the evolution of lipid oxidation with protein modification markers in all dispersion systems showed noteworthy attenuation of tryptophan fluorescence, protein carbonyl formation, and extensive polymerization of myosin in 2 h. This process preceded lipid oxidation which exhibited notable accumulations …