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Articles 151 - 170 of 170
Full-Text Articles in Life Sciences
Designer Receptors Enhance Memory In A Mouse Model Of Down Syndrome, Ashley M. Fortress, Eric D. Hamlett, Elena M. Vazey, Gary Aston-Jones, Wayne A. Cass, Heather A. Boger, Ann-Charlotte E. Granholm
Designer Receptors Enhance Memory In A Mouse Model Of Down Syndrome, Ashley M. Fortress, Eric D. Hamlett, Elena M. Vazey, Gary Aston-Jones, Wayne A. Cass, Heather A. Boger, Ann-Charlotte E. Granholm
Neuroscience Faculty Publications
Designer receptors exclusively activated by designer drugs (DREADDs) are novel and powerful tools to investigate discrete neuronal populations in the brain. We have used DREADDs to stimulate degenerating neurons in a Down syndrome (DS) model, Ts65Dn mice. Individuals with DS develop Alzheimer's disease (AD) neuropathology and have elevated risk for dementia starting in their 30s and 40s. Individuals with DS often exhibit working memory deficits coupled with degeneration of the locus coeruleus (LC) norepinephrine (NE) neurons. It is thought that LC degeneration precedes other AD-related neuronal loss, and LC noradrenergic integrity is important for executive function, working memory, and attention. …
Activation Of The Nf-Κb Pathway As A Mechanism Of Alcohol Enhanced Progression And Metastasis Of Human Hepatocellular Carcinoma, Fei Wang, Jin-Lian Yang, Ke-Ke Yu, Mei Xu, You-Zhi Xu, Li Chen, Yan-Min Lu, Hao-Shu Fang, Xin-Yi Wang, Zhong-Qian Hu, Fei-Fei Li, Lixin Kan, Jia Luo, Si-Ying Wang
Activation Of The Nf-Κb Pathway As A Mechanism Of Alcohol Enhanced Progression And Metastasis Of Human Hepatocellular Carcinoma, Fei Wang, Jin-Lian Yang, Ke-Ke Yu, Mei Xu, You-Zhi Xu, Li Chen, Yan-Min Lu, Hao-Shu Fang, Xin-Yi Wang, Zhong-Qian Hu, Fei-Fei Li, Lixin Kan, Jia Luo, Si-Ying Wang
Pharmacology and Nutritional Sciences Faculty Publications
BACKGROUND: Hepatocellular carcinoma (HCC), the most common form of primary liver cancer, is the third leading cause of cancer-related death in human. Alcohol is a known risk factor for HCC. However it is still unclear whether and how alcohol enhances the progression and metastasis of existing HCC.
METHODS AND RESULTS: We first retrospectively investigated 52 HCC patients (24 alcohol-drinkers and 28 non-drinkers), and found a positive correlation between alcohol consumption and advanced Tumor-Node-Metastasis (TNM) stages, higher vessel invasion and poorer prognosis. In vitro and in vivo experiments further indicated that alcohol promoted the progression and migration/invasion of HCC. Specifically, in …
Pact/Rax Regulates The Migration Of Cerebellar Granule Neurons In The Developing Cerebellum, Yue Yong, Ya Meng, Hanqing Ding, Zhiqin Fan, Yifen Tang, Chenghua Zhou, Jia Luo, Zun-Ji Ke
Pact/Rax Regulates The Migration Of Cerebellar Granule Neurons In The Developing Cerebellum, Yue Yong, Ya Meng, Hanqing Ding, Zhiqin Fan, Yifen Tang, Chenghua Zhou, Jia Luo, Zun-Ji Ke
Pharmacology and Nutritional Sciences Faculty Publications
PACT and its murine ortholog RAX were originally identified as a protein activator for the dsRNA-dependent, interferon-inducible protein kinase PKR. Recent studies indicated that RAX played a role in embryogenesis and neuronal development. In this study, we investigated the expression of RAX during the postnatal development of the mouse cerebellum and its role in the migration of cerebellar granule neurons (CGNs). High expression of RAX was observed in the cerebellum from postnatal day (PD) 4 to PD9, a period when the CGNs migrate from the external granule layer (EGL) to the internal granule layer (IGL). The migration of the EGL …
Structural Mechanism Of Laforin Function In Glycogen Dephosphorylation And Lafora Disease, Madushi Raththagala, M. Kathryn Brewer, Matthew W. Parker, Amanda R. Sherwood, Brian K. Wong, Simon Hsu, Travis M. Bridges, Bradley C. Paasch, Lance M. Hellman, Satrio Husodo, David A. Meekins, Adam O. Taylor, Benjamin D. Turner, Kyle D. Auger, Vikas V. Dukhande, Srinivas Chakravarthy, Pascual Sanz, Virgil L. Woods Jr., Sheng Li, Craig Vander Kooi, Matthew S. Gentry
Structural Mechanism Of Laforin Function In Glycogen Dephosphorylation And Lafora Disease, Madushi Raththagala, M. Kathryn Brewer, Matthew W. Parker, Amanda R. Sherwood, Brian K. Wong, Simon Hsu, Travis M. Bridges, Bradley C. Paasch, Lance M. Hellman, Satrio Husodo, David A. Meekins, Adam O. Taylor, Benjamin D. Turner, Kyle D. Auger, Vikas V. Dukhande, Srinivas Chakravarthy, Pascual Sanz, Virgil L. Woods Jr., Sheng Li, Craig Vander Kooi, Matthew S. Gentry
Molecular and Cellular Biochemistry Faculty Publications
Glycogen is the major mammalian glucose storage cache and is critical for energy homeostasis. Glycogen synthesis in neurons must be tightly controlled due to neuronal sensitivity to perturbations in glycogen metabolism. Lafora disease (LD) is a fatal, congenital, neurodegenerative epilepsy. Mutations in the gene encoding the glycogen phosphatase laforin result in hyperphosphorylated glycogen that forms water-insoluble inclusions called Lafora bodies (LBs). LBs induce neuronal apoptosis and are the causative agent of LD. The mechanism of glycogen dephosphorylation by laforin and dysfunction in LD is unknown. We report the crystal structure of laforin bound to phosphoglucan product, revealing its unique integrated …
Horticulturae — An International, Multidisciplinary, Open Access Journal, Douglas D. Archbold
Horticulturae — An International, Multidisciplinary, Open Access Journal, Douglas D. Archbold
Horticulture Faculty Publications
No abstract provided.
Zhx2 Enhances The Cytotoxicity Of Chemotherapeutic Drugs In Liver Tumor Cells By Repressing Mdr1 Via Interfering With Nf-Ya, Hongxin Ma, Xuetian Yue, Lifen Gao, Xiaohong Liang, Wenjiang Yan, Zhenyu Zhang, Haixia Shan, Hualin Zhang, Brett T. Spear, Chunhong Ma
Zhx2 Enhances The Cytotoxicity Of Chemotherapeutic Drugs In Liver Tumor Cells By Repressing Mdr1 Via Interfering With Nf-Ya, Hongxin Ma, Xuetian Yue, Lifen Gao, Xiaohong Liang, Wenjiang Yan, Zhenyu Zhang, Haixia Shan, Hualin Zhang, Brett T. Spear, Chunhong Ma
Microbiology, Immunology, and Molecular Genetics Faculty Publications
We previously reported the tumor suppressor function of Zinc-fingers and homeoboxes 2 (ZHX2) in hepatocellular carcinoma (HCC). Other studies indicate the association of increased ZHX2 expression with improved response to high dose chemotherapy in multiple myeloma. Here, we aim to test whether increased ZHX2 levels in HCC cells repress multidrug resistance 1(MDR1) expression resulting in increased sensitivity to chemotherapeutic drugs. We showed evidence that increased ZHX2 levels correlated with reduced MDR1 expression and enhanced the cytotoxicity of CDDP and ADM in different HCC cell lines. Consistently, elevated ZHX2 significantly reduced ADM efflux in HepG2 cells and greatly increased the CDDP-mediated …
Identification Of Micrornas In Two Species Of Tomato, Solanum Lycopersicum And Solanum Habrochaites, By Deep Sequencing, Shan-Shan Fan, Qian-Nan Li, Guang-Jun Guo, Jian-Chang Gao, Xiao-Xuan Wang, Yan-Mei Guo, John C. Snyder, Yong-Chen Du
Identification Of Micrornas In Two Species Of Tomato, Solanum Lycopersicum And Solanum Habrochaites, By Deep Sequencing, Shan-Shan Fan, Qian-Nan Li, Guang-Jun Guo, Jian-Chang Gao, Xiao-Xuan Wang, Yan-Mei Guo, John C. Snyder, Yong-Chen Du
Horticulture Faculty Publications
MicroRNAs (miRNAs) are ~21 nucleotide (nt), endogenous RNAs that regulate gene expression in plants. Increasing evidence suggests that miRNAs play an important role in species-specific development in plants. However, the detailed miRNA profile divergence has not been performed among tomato species. In this study, the small RNA (sRNA) profiles of Solanum lycopersicum cultivar 9706 and Solanum habrochaites species PI 134417 were obtained by deep sequencing. Sixty-three known miRNA families were identified from these two species, of which 39 were common. Further miRNA profile comparison showed that 24 known non-conserved miRNA families were species-specific between these two tomato species. In addition, …
Finding Our Way Through Phenotypes, Andrew R. Deans, Suzanna E. Lewis, Eva Huala, Salvatore S. Anzaldo, Michael Ashburner, James P. Balhoff, David C. Blackburn, Judith A. Blake, J. Gordon Burleigh, Bruno Chanet, Laurel D. Cooper, Mélanie Courtot, Sándor Csösz, Hong Cui, Wasila Dahdul, Sandip Das, T. Alexander Dececchi, Agnes Dettai, Rui Diogo, Robert E. Druzinsky, Michel Dumontier, Nico M. Franz, Frank Friedrich, George V. Gkoutos, Melissa Haendel, Luke J. Harmon, Terry F Hayamizu, Yongqun He, Heather M. Hines, Nizar Ibrahim, Laura M. Jackson, Pankaj Jaiswal, Christina James-Zorn, Sebastian Köhler, Guillaume Lecointre, Hilmar Lapp, Carolyn J. Lawrence, Nicolas Le Novère, John G. Lundberg, James Macklin, Austin R. Mast, Peter E. Midford, István Mikó, Christopher J. Mungall, Anika Oellrich, David Osumi-Sutherland, Helen Parkinson, Martín J. Ramírez, Stefan Richter, Peter N. Robinson, Alan Ruttenberg, Katja S. Schulz, Erik Segerdell, Katja C. Seltmann, Michael Sharkey, Aaron D. Smith, Barry Smith, Chelsea D. Specht, R. Burke Squires, Robert W. Thacker, Anne Thessen, Jose Fernandez-Triana, Mauno Vihinen, Peter D. Vize, Lars Vogt, Christine E. Wall, Ramona L. Walls, Monte Westerfeld, Robert A. Wharton, Christian S. Wirkner, James B. Woolley, Matthew J. Yoder, Aaron M. Zorn, Paula Mabee
Finding Our Way Through Phenotypes, Andrew R. Deans, Suzanna E. Lewis, Eva Huala, Salvatore S. Anzaldo, Michael Ashburner, James P. Balhoff, David C. Blackburn, Judith A. Blake, J. Gordon Burleigh, Bruno Chanet, Laurel D. Cooper, Mélanie Courtot, Sándor Csösz, Hong Cui, Wasila Dahdul, Sandip Das, T. Alexander Dececchi, Agnes Dettai, Rui Diogo, Robert E. Druzinsky, Michel Dumontier, Nico M. Franz, Frank Friedrich, George V. Gkoutos, Melissa Haendel, Luke J. Harmon, Terry F Hayamizu, Yongqun He, Heather M. Hines, Nizar Ibrahim, Laura M. Jackson, Pankaj Jaiswal, Christina James-Zorn, Sebastian Köhler, Guillaume Lecointre, Hilmar Lapp, Carolyn J. Lawrence, Nicolas Le Novère, John G. Lundberg, James Macklin, Austin R. Mast, Peter E. Midford, István Mikó, Christopher J. Mungall, Anika Oellrich, David Osumi-Sutherland, Helen Parkinson, Martín J. Ramírez, Stefan Richter, Peter N. Robinson, Alan Ruttenberg, Katja S. Schulz, Erik Segerdell, Katja C. Seltmann, Michael Sharkey, Aaron D. Smith, Barry Smith, Chelsea D. Specht, R. Burke Squires, Robert W. Thacker, Anne Thessen, Jose Fernandez-Triana, Mauno Vihinen, Peter D. Vize, Lars Vogt, Christine E. Wall, Ramona L. Walls, Monte Westerfeld, Robert A. Wharton, Christian S. Wirkner, James B. Woolley, Matthew J. Yoder, Aaron M. Zorn, Paula Mabee
Entomology Faculty Publications
Despite a large and multifaceted effort to understand the vast landscape of phenotypic data, their current form inhibits productive data analysis. The lack of a community-wide, consensus-based, human- and machine-interpretable language for describing phenotypes and their genomic and environmental contexts is perhaps the most pressing scientific bottleneck to integration across many key fields in biology, including genomics, systems biology, development, medicine, evolution, ecology, and systematics. Here we survey the current phenomics landscape, including data resources and handling, and the progress that has been made to accurately capture relevant data descriptions for phenotypes. We present an example of the kind of …
Pack Factor Measurements For Corn In Grain Storage Bins, Rumela Bhadra, Aaron P. Turner, Mark E. Casada, Michael D. Montross, Sidney A. Thompson, Josephine M. Boac, Samuel G. Mcneill, Ronaldo G. Maghirang
Pack Factor Measurements For Corn In Grain Storage Bins, Rumela Bhadra, Aaron P. Turner, Mark E. Casada, Michael D. Montross, Sidney A. Thompson, Josephine M. Boac, Samuel G. Mcneill, Ronaldo G. Maghirang
Biosystems and Agricultural Engineering Faculty Publications
Shelled yellow corn is commonly stored in concrete or corrugated steel bins. Granular materials compact under their own weight, primarily due to particle rearrangement, leading to an increase in bulk density and a change in volume when stored. Reliable grain pack factors are needed to estimate storage capacities and to accurately monitor grain inventories. A science-based model (WPACKING) of pack factors is available that uses the differential form of Janssen's equation and takes into account the variation in density caused by pressure variation with height and moisture content of the grain and accounts for the effects of grain type, test …
Reductions Of Wheat Yield And Yield Components And Nitrogen Loss Following Frozen Soil Nitrogen Applications, Carrie Ann Knott, Edwin L. Ritchey, Lloyd W. Murdock
Reductions Of Wheat Yield And Yield Components And Nitrogen Loss Following Frozen Soil Nitrogen Applications, Carrie Ann Knott, Edwin L. Ritchey, Lloyd W. Murdock
Plant and Soil Sciences Research Report
Most wheat producers in Kentucky apply nitrogen (N) as a split application. The first N increment is applied when wheat plants begin actively growing (green-up) in late winter, which is typically in mid- February between growth stages Feekes 2 to 3. The second N increment typically occurs in March when wheat is between Feekes 5 to 6. Many producers in Kentucky, especially Western Kentucky, have become accustomed to beginning first N applications in late January when the ground is frozen and the wheat is still dormant. This practice allows them to apply N to large acreages of wheat while avoiding …
An Optical Sprayer Nozzle Flow Rate Sensor, Joseph S. Dvorak, Luke E. Bryant
An Optical Sprayer Nozzle Flow Rate Sensor, Joseph S. Dvorak, Luke E. Bryant
Biosystems and Agricultural Engineering Faculty Publications
Ensuring proper flow rates from each nozzle on an agricultural sprayer has become even more important as advances continue to be made in precision application technology. In this article, we describe the structure and testing of a sensor technology based on optical cross-correlation to determine the flow rate of individual sprayer nozzles. An advantage of this technology is that it does not require that impellers or other components be placed in the flow, which could cause plugging. The only moving part in the entire system is a solenoid used to inject a tracer dye. The objective of this study was …
Control System Development And Response Analysis Of An Electronically Actuated Variable-Orifice Nozzle For Agricultural Pesticide Applications, Joe D. Luck, Scott A. Shearer, Michael P. Sama, Santosh K. Pitla
Control System Development And Response Analysis Of An Electronically Actuated Variable-Orifice Nozzle For Agricultural Pesticide Applications, Joe D. Luck, Scott A. Shearer, Michael P. Sama, Santosh K. Pitla
Biosystems and Agricultural Engineering Faculty Publications
The goal of this research project was to further the development of an electromechanically controlled variable-orifice nozzle by creating an electronic control system and then evaluating that system based on step and ramp inputs. The control system was developed in a programming environment that combined an electronic data acquisition system and actuator with pressure and flow sensors. A proportional, variable-gain (based on system pressure) control system was developed to adjust nozzle flow rates to meet target application rates. The constraints were to achieve settling time of less than 1.0 s, overshoot of less than 10% of maximum flow (or minimum …
A Therapeutic Approach For Senile Dementias: Neuroangiogenesis, Charles T. Ambrose
A Therapeutic Approach For Senile Dementias: Neuroangiogenesis, Charles T. Ambrose
Microbiology, Immunology, and Molecular Genetics Faculty Publications
Alzheimer's disease (AD) and related senile dementias (SDs) represent a growing medical and economic crisis in this country. Apart from cautioning persons about risk factors, no practical, effective therapy is currently available. Much of the recent research in AD has been based on the amyloid cascade theory. Another approach assumes a vascular basis for SDs. This paper presents evidence from a score of studies that cerebral capillary density (CCD) declines during old age in animals and people as well as in AD. Neuroangiogenic (NAG) factors initiate and maintain capillaries in the brain. Thus a waning level of these factors and …
Forage News [2015-01], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News [2015-01], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News
- Forages at KCA
- 2015 Kentucky Small Ruminant Grazing Conference
- 35th Kentucky Alfalfa Conference
- 2014 Kentucky Review
- US Red Meat & Poultry Consumption
- Alfalfa Seed Supply Adequate for 2015
- Eight New Alfalfas are Approved by Variety Board
The Effects Of Economic Conditions On The Sport Horse Industry In Kentucky And Nationally, Elizabeth Lampert
The Effects Of Economic Conditions On The Sport Horse Industry In Kentucky And Nationally, Elizabeth Lampert
Lewis Honors College Capstone Collection
The horse industry in Kentucky is larger than thoroughbreds and racing. Competing sport horses is an increasingly popular form of riding and showing in the bluegrass and nationwide. Sport horse disciplines include dressage, eventing, and hunter-jumper horses. In Kentucky, sport horse enthusiasts are fortunate enough to be near the Kentucky Horse Park, a park known worldwide for its shows and courses. In 2010, the park hosted the World Equestrian Games, marking the first time that the World Equestrian Games had been held outside of Europe. Focusing mainly on the bluegrass and the nation as a whole, the effects of the …
Smooth-Muscle Bmal1 Participates In Blood Pressure Circadian Rhythm Regulation, Zhongwen Xie, Wen Su, Shu Liu, Guogang Zhao, Karyn Esser, Elizabeth A. Schroder, Mellani Lefta, Harald M. Stauss, Zhenheng Guo, Ming Cui Gong
Smooth-Muscle Bmal1 Participates In Blood Pressure Circadian Rhythm Regulation, Zhongwen Xie, Wen Su, Shu Liu, Guogang Zhao, Karyn Esser, Elizabeth A. Schroder, Mellani Lefta, Harald M. Stauss, Zhenheng Guo, Ming Cui Gong
Physiology Faculty Publications
As the central pacemaker, the suprachiasmatic nucleus (SCN) has long been considered the primary regulator of blood pressure circadian rhythm; however, this dogma has been challenged by the discovery that each of the clock genes present in the SCN is also expressed and functions in peripheral tissues. The involvement and contribution of these peripheral clock genes in the circadian rhythm of blood pressure remains uncertain. Here, we demonstrate that selective deletion of the circadian clock transcriptional activator aryl hydrocarbon receptor nuclear translocator-like (Bmal1) from smooth muscle, but not from cardiomyocytes, compromised blood pressure circadian rhythm and decreased blood …
Stored Grain Pack Factors For Wheat: Comparison Of Three Methods To Field Measurements, Josephine M. Boac, Rumela Bhadra, Mark E. Casada, Sidney A. Thompson, Aaron P. Turner, Michael D. Montross, Samuel G. Mcneill, Ronaldo G. Maghirang
Stored Grain Pack Factors For Wheat: Comparison Of Three Methods To Field Measurements, Josephine M. Boac, Rumela Bhadra, Mark E. Casada, Sidney A. Thompson, Aaron P. Turner, Michael D. Montross, Samuel G. Mcneill, Ronaldo G. Maghirang
Biosystems and Agricultural Engineering Faculty Publications
Storing grain in bulk storage units results in grain packing from overbearing pressure, which increases grain bulk density and storage unit capacity. This study compared pack factors of hard red winter (HRW) wheat in vertical storage bins using different methods: the existing packing model (WPACKING), the USDA Risk Management Agency (RMA) method, and the USDA Farm Service Agency Warehouse Licensing and Examination Division (FSA-W) method. Grain bins containing HRW wheat were measured in Kansas, Oklahoma, and Texas. Packing was measured in corrugated steel bins and reinforced concrete bins with diameters ranging from 4.6 to 31.9 m (15.0 to 104.6 ft) …
Flow, Spray Pattern, And Droplet Spectra Characteristics Of An Electronically Actuated Variable-Orifice Nozzle, Joe D. Luck, Santosh K. Pitla, Michael P. Sama, Scott A. Shearer
Flow, Spray Pattern, And Droplet Spectra Characteristics Of An Electronically Actuated Variable-Orifice Nozzle, Joe D. Luck, Santosh K. Pitla, Michael P. Sama, Scott A. Shearer
Biosystems and Agricultural Engineering Faculty Publications
The purpose of this study was to develop and evaluate the flow rate, spray pattern, and droplet spectra characteristics of an actively controlled variable-orifice nozzle at constant carrier pressures. A commercially available variable-orifice nozzle (VariTarget) was modified to allow for direct electromechanical control of the metering stem. The modified system was tested at five carrier pressures ranging from 138 to 414 kPa and five metering stem (and thus orifice) positions. The metering stem position range was chosen because it provided a linear response in flow rate at each carrier pressure. Flow rate testing indicated a turndown ratio of 2.4:1 at …
Kentucky Forestry Economic Impact Report 2014, Jeff Stringer, Billy Thomas, Bobby Ammerman, Alison Davis
Kentucky Forestry Economic Impact Report 2014, Jeff Stringer, Billy Thomas, Bobby Ammerman, Alison Davis
Kentucky Forestry Economic Impact Reports
Forests cover one-half of Kentucky and provide a wide range of benefits to the commonwealth. One of the most notable benefits is the economic contribution that we gain from the utilization of timber and wood resources. Analysis of Kentucky’s forest and wood industries in 2014 indicated an estimated direct economic impact of $8.3 billion (up 4.4% from 2013). These industries employed over 28,200 individuals. Total economic impacts were estimated at $12.8 billion and 57,700 jobs in 2014. Kentucky is one of the leading producers of hardwood forest products in the south and exports wood products across the nation and the …
Kentucky Water Resources Research Institute Annual Technical Report Fy 2014, Kentucky Water Resources Research Institute, University Of Kentucky
Kentucky Water Resources Research Institute Annual Technical Report Fy 2014, Kentucky Water Resources Research Institute, University Of Kentucky
KWRRI Annual Technical Reports (USGS’s 104b Grant Program)
The 2014 Annual Technical Report for Kentucky consolidates reporting requirements for the Section 104(b) base grant award into a single document that includes: 1) a synopsis of each research project that was conducted during the period, 2) citations for related publications, reports, and presentations, 3) a description of information transfer activities, 4) a summary a student support during the reporting period, and 5) notable awards and achievements during the year.