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Impacts Of T-Phylloplanin Gene Knockdown And Of Helianthus And Datura Phylloplanins On Peronospora Tabacina Spore Germination And Disease Potential, Antoaneta B. Kroumova, Ryan W. Shepherd, George J. Wagner
Impacts Of T-Phylloplanin Gene Knockdown And Of Helianthus And Datura Phylloplanins On Peronospora Tabacina Spore Germination And Disease Potential, Antoaneta B. Kroumova, Ryan W. Shepherd, George J. Wagner
Plant and Soil Sciences Faculty Publications
T-phylloplanin proteins secreted to aerial surfaces of tobacco (Nicotiana tabacum) by short procumbent trichomes inhibit spore germination and blue mold disease caused by the oomycete pathogen Peronospora tabacina. Many other plants were found to contain water-washed leaf surface proteins (phylloplanins), but the functions and properties of these are not known. Here we extend earlier evidence for the antifungal activity of T-phylloplanins using a reverse genetics approach. RNA interference of the T-phylloplanin gene in tobacco ‘T.I. 1068’ resulted in loss of T-phylloplanin mRNA and protein, loss of in vitro spore germination inhibition activity, and leaf infection …