Life Sciences Commons^™

Determining The Relationship Of Human Enteric Viruses In Clinical, Wastewater, And Environmental Samples Utilizing Molecular And Cell Culture Techniques, Jacquelina Susann Williams Woods

Dissertations

This study was the first to examine five significant enteric viruses in human fecal material, sewage, and oysters to show a genetic relationship between human enteric viruses and different sample matrices. Fecal samples were collected from an area hospital and examined for norovirus genotype I (NoV GI), norovirus genotype II (NoV Gil), hepatitis A virus (HA V), adenovirus (ADV), and enteroviruses. During this study, sewage samples were collected from a Waster Water Treatment Plant (WWTP) in Mobile, ALand oyster sentinels were placed at 0.1 nautical miles (nm) (station 1 ), 0.2nm (station 2}, 1.5nm (station 3), and 4nm (station 4) …

Development Of Representative Species-Level Molecular Markers And Morphological Character Analysis Of Leucothoid Amphipods (Crustacea: Amphipoda), Kristine Nicolle White

Dissertations

Leucothoid amphipods were investigated using morphology and molecular rDNA gene sequence fragments. The morphological diagnostic characters for traditional taxonomy have been clarified, a molecular marker for representative species has been developed, and one of the current anamorph-leucomorph connections has been confirmed with molecular sequence data. Ultimately this study has combined traditional morphological and modern molecular methods to clarify the taxonomy and to propose a preliminary phylogeny of the Leucothoidae. Analysis of 18S rDNA gene fragments from 13 species in two genera supported the current morphological species designations and the separation of the family into two clades. Combined analysis of 18S …

Systematic Revision Of The Kalliapseudidae (Crustacea: Peracarida: Tanaidacea) And The Population Genetic Structure And Phylogeography Of A Species Along The Southeastern And Gulf Coasts Of North America, David Thomas Drumm

Dissertations

The Kalliapseudidae is a family of shallow burrow-dwelling marine and estuarine tanaidaceans. There are currently 39 known species of 12 genera and three subfamilies. They are primarily circumtropical in distribution; however, several species are found in temperate waters. The phylogeny of Kalliapseudidae is assessed to test the monophyly of currently accepted subfamilies and genera, based largely on examination of material loaned from various museums and institutions. Multiple exemplars from other apseudomorph families were also included in the ingroup to test the monophyly of the family. Parsimony analyses included 41 terminal taxa and 64 binary and multistate morphological characters. Analyses based …

Soccer Penalty Kick Pre-Impact Cues Of Male Right-Footed High School Soccer Players, Jason Ryan Jones

Dissertations

The purpose of this study was to use direct observation methods to collect evidence of pre-impact cues in a soccer penalty kick and to analyze their predictive powers in determining shot direction. The five pre-impact cues were position of the approachru n, alignmento f the hips,leano ftrunk, placemenot f non-kickingf oot, and actiono f the non-kickings idea rm.V ideo wasc ollecteda ndt hena nalyzedu singa Dartfishrn tagging panel. There were a total of fifty male high school right-footed participants.C oachesfr om areah igh schools occert eamsp rovidedt heir top penaltyk ick takerst o participatein the study.V ideo wasc ollectedo n …

The Natural Provenance: Ecoliteracy In Higher Education In Mississippi, Sarah Elizabeth Wheeless

Dissertations

Researchers have suggested that there is an increasing apathy in the study of natural history both in academic settings and in the scientific community (Schmidly, 2005). Natural history is the cornerstone of ecological literacy. However, most studies of environmental knowledge do not directly address knowledge of local natural history. Instead, they concern knowledge of human environmental issues, environmental concepts, or broad ecological knowledge. Ecoliteracy established the study of natural history as fundamental to environmental knowledge and seeks to determine levels of knowledge of local environments and factors associated with that knowledge (Pilgrim et al., 2007).

This study investigated ecoliteracy in …

Role Of Casein Kinase 1 In The Glucose Sensor-Mediated Signaling Pathway In Yeast, Satish Pasula, Samujjwal Chakraborty, Jae H. Choi, Jeong-Ho Kim

Faculty Publications

Background

In yeast, glucose-dependent degradation of the Mth1 protein, a corepressor of the glucose transporter gene (HXT) repressor Rgt1, is a crucial event enabling expression of several HXT. This event occurs through a signaling pathway that involves the Rgt2 and Snf3 glucose sensors and yeast casein kinase 1 and 2 (Yck1/2). In this study, we examined whether the glucose sensors directly couple with Yck1/2 to convert glucose binding into an intracellular signal that leads to the degradation of Mth1.

Results

High levels of glucose induce degradation of Mth1 through the Rgt2/Snf3 glucose signaling pathway. Fluorescence microscopy analysis …

Beyond Affymetrix Arrays: Expanding The Set Of Known Hybridization Isotherms And Observing Pre-Wash Signal Intensities, Alexander Pozhitkov, Idrissa Boube, Marius Brouwer, Peter A. Noble

Faculty Publications

Microarray hybridization studies have attributed the nonlinearity of hybridization isotherms to probe saturation and post-hybridization washing. Both processes are thought to distort 'true' target abundance because immobilized probes are saturated with excess target and stringent washing removes loosely bound targets. Yet the paucity of studies aimed at understanding hybridization and dissociation makes it difficult to align physicochemical theory to microarray results. To fill the void, we first examined hybridization isotherms generated on different microarray platforms using a ribosomal RNA target and then investigated hybridization signals at equilibrium and after stringent wash. Hybridization signal at equilibrium was achieved by treating the …

Functional Genomics Tool: Gene Silencing In Ixodes Scapularis Eggs And Nymphs By Electroporated Dsrna, Shahid Karim, Emily Troiano, Thomas N. Mather

Faculty Publications

Background

Ticks are blood-sucking arthropods responsible for transmitting a wide variety of disease-causing agents, and constitute important public health threats globally. Ixodes scapularis is the primary vector of the Lyme disease agent in the eastern and central U.S. RNAi is a mechanism by which gene-specific double-stranded RNA (dsRNA) triggers degradation of homologous mRNA transcripts. Here, we describe an optimized protocol for effectively suppressing gene expression in the egg and nymphal stages of I. scapularis by electroporation.

Results

The genes encoding the putative Phospholipase A₂ (PLA₂), cytoplasmic Cystatin, Syntaxin-5, β-Actin and Calreticulin were targeted by delivering the dsRNA …

Environmental Correlates Of Abundances Of Mosquito Species And Stages In Discarded Vehicle Tires, Donald A. Yee, Jamie M. Kneitel, Steven A. Juliano

Faculty Publications

Discarded vehicle tires are a common habitat for container mosquito larvae, although the environmental factors that may control their presence or abundance within a tire are largely unknown. We sampled discarded vehicle tires in six sites located within four counties of central Illinois during the spring and summer of 2006 to determine associations between a suite of environmental factors and community composition of container mosquitoes. Our goal was to find patterns of association between environmental factors and abundances of early and late instars. We hypothesized that environmental factors correlated with early instars would be indicative of oviposition cues, whereas environmental …

Constructing A Fish Metabolic Network Model, Shuzhao Li, Alexander Pozhitkov, Rachel A. Ryan, Charles Steve Manning, Nancy J. Brown-Peterson, Marius Brouwer

Faculty Publications

We report the construction of a genome-wide fish metabolic network model, MetaFishNet, and its application to analyzing high throughput gene expression data. This model is a stepping stone to broader applications of fish systems biology, for example by guiding study design through comparison with human metabolism and the integration of multiple data types. MetaFishNet resources, including a pathway enrichment analysis tool, are accessible at http://metafishnet.appspot.com.

First Report Of Viral Pathogens Wssv And Ihhnv In Argentine Crustaceans, Sergio R. Martorelli, Robin M. Overstreet, Jean A. Jovonovich

Faculty Publications

The pathogenic penaeid shrimp viruses white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV) are reported for the first time from Argentina. They both occurred in wild crustaceans in Bahia Blanca Estuary, with WSSV detected by polymerase chain reaction (PCR) or real time quantitative PCR (qPCR) methods as infecting samples as high as 56% of the penaeid Artemesia longinaris Bate, 1888, 67% of the grapsoid Cyrtograpsus angulatus Dana, 1851, and 40% of the introduced palaemonid Palaemon macrodactylus Rathbun, 1902. The highest detected copy number was 39,600 copies/mu g DNA. IHHNV was tested for using PCR in …

Endohelminths Of A Snake Mackerel, Gempylus Serpens (Trichiuroidea: Gempylidae), From The Gulf Of Mexico, Charles K. Blend, Norman O. Dronen, James S. Franks, George W. Benz

Gulf and Caribbean Research

Endohelminths are reported from a female snake mackerel, Gempylus serpens (Trichiuroidea: Gempylidae), captured from a depth of 61 m in the Gulf of Mexico 140 km south of the mouth of Mobile Bay, AL, in August 1998. A diverse endohelminth parasite fauna was found: 29 plerocercoid type I tetraphyllideans from the lower intestine; 4 didymozoid metacercariae allocated to the collective group Monilicaecum and one didymozoid metacercaria of the collective group Torticaecum from the pyloric cecum; one juvenile Gonocerca phycidis from the stomach; and 5 larvae (L3 stage) comprising 3 species of Anisakis from the pyloric cecum. These nematodes were identified …

Notes On The Biology Of An Adult Female Chimaera Cubana Captured Off St. Croix, U.S. Virgin Islands, William B. Driggers Iii, Jill M. Hendon, Michael J. Andres, Stephen S. Curran, Christopher T. Gledhill, Mark A. Grace, Michael D. Hendon, Christian M. Jones, Brandi T. Noble, Kevin R. Rademacher

Gulf and Caribbean Research

Within the western North Atlantic Ocean there are at least 4 genera and 5 species of chimaeroids occurring in deep waters generally associated with outer continental slopes or areas of high bathymetric relief (Didier 2002; Didier 2004). Two chimaeroids, Chimaera cubana and Hydrolagus alberti, are known to be indigenous to the Caribbean Sea in waters associated with the Greater and Lesser Antilles. While H. alberti occurs throughout the Gulf of Mexico and the Caribbean Sea, C. cubana is thought to be endemic to an area bounded by Cuba and Colombia (IUCN 2009). These two chimaeras are readily differentiated by …

Observations On The Kalliapseudid Tanaidacea (Crustacea: Malacostraca: Peracarida) From The Northwestern Atlantic, With An Illustrated Key To The Species, David T. Drumm, Richard W. Heard

Gulf and Caribbean Research

New information for the kalliapseudid Tanaidacea occurring in the northwestern Atlantic is presented and discussed, including data on range extensions and new depth ranges for 4 species. The taxa studied came from the shelf and coastal waters of the southeastern United States, Puerto Rico and Trinidad. The occurrence of Mesokalliapseudes bahamensis Sieg is extended from the Bahamas and Belize to the coastal waters of East and Gulf coasts (South Carolina to West Florida). The range of Psammokalliapseudes granulosus Brum is expanded northward into the eastern Gulf of Mexico and new locality records for this species are established for Tobago and …

Recruitment And Colonization Of Macroalgae To A Newly Constructed Rocky Intertidal Habitat In The Northwest Gulf Of Mexico, Ryan L. Fikes, Roy L. Lehman

Gulf and Caribbean Research

Marine macroalgal assemblages on artificial structures play an important ecological role in coastal and estuarine ecosystems and may supplement natural communities in nearby waters. The rocky jetties of Packery Channel, located near Corpus Christi, Texas represent a recent addition of hard structure for colonization in the northwest Gulf of Mexico. The purpose of this research was to monitor the initial immigration of macroalgal species during the first year of colonization and determine the effects of wave energy on recruitment. Ten sampling sites were established along the offshore portion of the new Packery Channel jetties. Samples were taken bimonthly from along …

Evaluating Management Actions For Spotted Seatrout, Cynoscion Nebulosus, In Mississippi With An Age-Structured Projection Model, Richard S. Fulford, J. Read Hendon

Gulf and Caribbean Research

Spotted seatrout, Cynoscion nebulosus, is an important recreational fishery in the coastal waters of the Gulf of Mexico and is the most sought after gamefish in coastal Mississippi. The management of C. nebulosus is state-specific, and unlike other similarly managed species, data on both population structure and movement support the existence of local sub-stocks. It is important for each state to clearly examine its own sub-stock in the context of its own state fishery in order to properly manage for local sustainability. We used an age-structured assessment model to examine the status (1993–2005) of the Mississippi C. nebulosus population …

Effects Of Hurricane Katrina On An Incipient Population Of Giant Salvinia Salvinia Molesta In The Lower Pascagoula River, Mississippi, Pam L. Fuller, Mike G. Pursley, Dale Diaz, Wesley Devers

Gulf and Caribbean Research

The objectives of this study were to: 1) survey the lower Pascagoula River Basin and determine the post–storm distribution and abundance of giant salvinia; 2) control any remaining giant salvinia through physical and/or chemical means; 3) determine the fate of the bio–control agents; and 4) determine if re–introduction of salvinia weevils is needed and if so, to decide where best to release them.

Observations Of A Black Grouper (Mycteroperca Bonaci) Spawning Aggregation In Bermuda, Brian E. Luckhurst

Gulf and Caribbean Research

Diving observations at a black grouper (Mycteroperca bonaci) spawning aggregation site on Bermuda’s reef platform revealed many similarities to observations of this species obtained at multi-species spawning aggregation sites in Belize. In addition to similarities in body sizes, color patterns and some behavior, the principal spawning period in the days after the full moon was also similar. Although spawning was not observed in this study, there was ample indirect evidence of spawning at the site, i.e. courtship behavior by males, females with distended abdomens, and color changes. The formation of temporary spawning territories by males and courtship behavior …

Growth Patterns Of Shoal Grass Halodule Wrightii And Manatee Grass Syringodium Filiforme In The Western Gulf Of Mexico, Melissa A. Gutierrez, Annette A. Cardona, Delbert L. Smee

Gulf and Caribbean Research

Although manatee grass is becoming increasingly abundant in Texas bays, its growth characteristics have not been measured in the western GOM. Changes in seagrass species composition can have significant community effects (Micheli et al. 2008), but the effects of a transition from shoal to manatee grass in the LM have not been extensively studied (but see Tolan et al. 1997). The goals of our study were to measure growth patterns of these two seagrass species in two locations in the western GOM that vary in salinity, epiphyte loads, and nutrient inputs.

Do Small, Patchy, Constructed Intertidal Oyster Reefs Reduce Salt Marsh Erosion As Well As Natural Reefs?, Alix G. Stricklin, Mark S. Peterson, John D. Lopez, Christopher A. May, Christina F. Mohrman, Mark S. Woodrey

Gulf and Caribbean Research

One ecological service that oyster reefs provide is stabilization of shorelines through reduced wave energy and erosion from boat traffic, storms, and predominant wind direction. Additionally, increasing sedimentation can enhance the growth of emergent marsh vegetation which further stabilizes unconsolidated sediments. A 21 mo study of constructed (with only 30-35% coverage) and natural oyster reefs in 3 bayous in the Grand Bay National Estuarine Research Reserve (NERR) suggested constructed reefs benefit this retrograding deltaic ecosystem. The marsh edge adjacent to all constructed reefs was less eroded (mean = 0.043 m) than edges adjacent to natural reefs (mean = 0.728 m), …