Life Sciences Commons^™

Survival, Abundance, And Relative Predation Of Wild Rainbow Trout In The Deerfield Reservoir System, South Dakota, Jeremy L. Kientz

Electronic Theses and Dissertations

Rainbow Trout Oncorhynchus mykiss are routinely stocked in Black Hills streams and reservoirs to enhance angling opportunities for the public, however in most cases, hatchery-reared Rainbow Trout do not successfully recruit to establish natural populations. One exception is the Deerfield Reservoir system, where it is estimated that up to 25% of the Rainbow Trout population consists of naturally produced, wild Rainbow Trout. While recruitment of wild Rainbow Trout to the Deerfield Reservoir fishery does occur, annual stockings of 12,000 hatchery Rainbow Trout have continued. In recent years, adipose fin clips were used to identify hatchery Rainbow Trout stocked into Deerfield …

The Contribution Of Missouri River Reservoir Side-Channel And Floodplain Habitats To Mainstem Fish Populations: The Effects Of Losing Connectivity Between Hipple Lake And Lake Sharpe, William Joseph Radigan

Electronic Theses and Dissertations

Catastrophic flooding of the Missouri River in 2011 has had lasting effects on floodplain habitats (i.e. floodplain lakes) and side-channel habitats (e.g. canals, side-channel embayments, stilling basins, and tributaries) in Lake Sharpe, SD. Floodplain and side-channel habitats are rare habitat in Lake Sharpe, a mainstem Missouri River reservoir, and are thought to be crucial habitat for prey and sport fish. Hipple Lake, the only warm-water floodplain embayment in Lake Sharpe, is in danger of losing connectivity to the reservoir because of sedimentation resulting from the 2011 flood. To evaluate Hipple Lake’s natal and adult contribution to Lake Sharpe’s fishery, otolith …

Evaluation Of Tillage, Crop Rotation, And Cover Crop Impacts On Corn Nitrogen Requirements In Southeastern South Dakota, Sara Louise Berg

Electronic Theses and Dissertations

Nitrogen (N) is a vital factor of corn (Zea mays) production. Previous work in South Dakota has shown that there is uncertainty as to whether nitrogen requirements are the same for corn raised under no-till (NT) versus conventional till (CT) production systems. The objective of this study was to evaluate whether N requirements continue to be greater under long-term NT versus CT production systems in southeastern South Dakota, while also considering effects from cover crops and crop rotation. This was a two year study conducted at the SDSU Southeast Research Farm near Beresford, SD; it was superimposed on a long-term …

Inactivation Of Thermodynamic Sporeformers And Spores In Skim Milk By Continuous Ultrasonication And Hydrodynamic Cavitation In Combination With Thermal Treatments, Dikshi Bawa

Electronic Theses and Dissertations

Studies were conducted to determine the effectiveness of ultrasonication and hydrodynamic cavitation, in a continuous mode, alone and in combination with thermal treatments, on heat resistant aerobic sporeformers and spores in skim milk, and skim milk concentrate. The first study was conducted to investigate the effectiveness of a continuous ultrasonication process to reduce thermoduric Bacillus coagulans cells in skim milk. A 25% reduction was obtained by lab pasteurization alone, whereas ultrasonication alone resulted in an inactivation of 92% after 12 passes (80 sec exposure time per pass). Ultrasonication when combined with pasteurization resulted in further increased inactivation to 99.98%. It …

The Endophytes Of Pediomelum Esculentum: A Unique Case In Legume Evolution, Tyrel Ryan Deutscher

Electronic Theses and Dissertations

Pediomelum esculentum (commonly prairie turnip) is a perennial legume of the Great Plains, consisting of a deep taproot and large edible tuber, and has served as a nutritious staple in Native American diets. The tuber is capable of storing up to 20 percent protein by weight. P. esculentum is a legume, but not a prominent nodule former; instead, it grows in nitrogen-limited soils and produces large amounts of protein. This suggests the involvement of biological nitrogen fixation. We have investigated the presence of diazotrophic endophytes in P. esculentum. Bacteria were isolated from wild plants on nitrogen free media, identified …

Functional Effects Of Calcium Regulation Of Thin Filaments At Single Particle Resolution, Christopher Solis-Ocampo

Electronic Theses and Dissertations

Heart disease is the leading cause of death in the United States. Understanding heart function at the molecular level is critical for developing of more effective treatments. In the cardiac muscle, the thin filament is composed by troponin (Tn), tropomyosin (Tm), and F-actin. It provides Ca2+-dependent regulation of contraction by modulating myosin attachment and force generation in a cooperative scheme. However, this mechanism remains unclear. To understand thin filament activation, we studied the binding and functional properties of Tn and Tm to F-actin at single particle resolution by employing fluorescence image colocalization, in vitro motility assays, and Förster resonance energy …

Role Of Exopolymeric Substances In Biofilm Formation On Dairy Separation Membranes, Nuria Garcia-Fernandez

Electronic Theses and Dissertations

In the concentration of cheese whey by membrane filtration processes, fouling is a major limiting factor that leads to flux decline and affects product quality. The growth of biofilms on membranes contributes to biofouling. Biofilms are very difficult to eliminate, and will serve as the seed for additional biofilm development and product contamination. The biofilm matrix is composed mainly of microbial exopolymeric substances (EPS), and therefore EPS is thought to help bacteria to adhere and form biofilms by acting as a cementing agent. The first research project under this study was conducted with the aim of elucidating the role of …

An Analysis Of The Demand For Rail Transportation Of Corn, Soybeans, And Wheat In South Dakota, Melinda Sommer

Electronic Theses and Dissertations

The cropping patterns, grain marketing flows, and transportation needs have changed in South Dakota as increased ethanol production has led to a significant increase in corn production and the production of distiller’s dried grains with solubles (DDGS) as a co-product. Previous studies have not focused specifically on the grain and oilseed transportation needs in South Dakota over an extended period of time that covers the influence of ethanol production. Quarterly rail data from the Surface Transportation Board Public Use Waybill Sample for 1991 to 2013 were used in a regression analysis of the demand for rail transportation of corn, soybeans, …

A Fret Investigation Into Molecular Mechanisms Of Cardiac Troponin Activation In Reconstituted Thin Filaments, Maria Eleni Moutsoglou

Electronic Theses and Dissertations

Cardiomyopathies (CM) are the leading cause of death in America, and can develop from mutations in sarcomeric proteins, leading to altered protein structure and function. Current therapies target upstream signaling pathways to treat the symptoms of heart failure, but are associated with increased mortality by affecting downstream signaling pathways and other muscle types. Rational drug design can develop therapies to treat CM at the protein level. However, a detailed knowledge of how sarcomeric proteins regulate muscle contraction is required. Muscle contraction occurs through a cyclic interaction between actin thin and myosin thick filaments, regulated by intracellular Ca2+ concentration. Troponin (Tn), …

Growth Performance, Nutrient Utilization, And Metabolic Profile Of Dairy Heifers Fed Diets High In Distillers Grains With Different Forage To Concentrate Ratios, Angela Kristia Manthey

Electronic Theses and Dissertations

Two studies were conducted to evaluate the effect of limit-feeding heifers distillers grains with solubles (DDGS) with varying forage to concentrate ratios. The effects on growth, feed efficiency, rumen fermentation, nutrient digestibility, metabolic profile and onset of puberty, as well as post trial performance were investigated. First, a 16-wk feeding trial was conducted using 48 Holstein heifers to evaluate effects of dietary treatment on dry matter intake (DMI), average daily gain (ADG), growth performance, rumen fermentation, and nutrient digestibility. Treatments were 1) 30% DDGS, with the diet fed at 2.65% of body weight (BW) (30DG), 2) 40% DDGS, with the …

Icook 4-H: 0 To 24-Month Accelerometer-Derived Physical Activity And Sedentary Time In Youth, Emily Hofer

Electronic Theses and Dissertations

To assess accelerometer-derived physical activity and sedentary time from 0 to 24- months in youth in the iCook 4-H program. The iCook 4-H Program was a 5-state, randomized, control-treatment, family-based childhood obesity prevention intervention promoting cooking, eating and playing together. Youth, 9-10 years old, and their main adult meal preparer, participated in the 12-week program followed by monthly newsletters and bi-yearly booster sessions until 24-months. Physical activity and sedentary time were determined for youth who wore an Actigraph GT3X+ accelerometer for 7 days at 0, 4, 12, and 24-months and met defined accelerometer compliance standards. Mean daily minutes of sedentary …

Raptors In Temperate Grasslands: Ecology Of Ferruginous Hawk, Golden Eagle, And Northern Harrier In The Northern Great Plains, Shubham Datta

Electronic Theses and Dissertations

Ferruginous hawks (Buteo regalis) are a grassland and shrubland obligate nesting raptor and prefer lightly grazed pasture or idle areas for nesting. Their population reportedly declines in number if more than 30% of an area is cultivated and they rarely nest in areas dominated by croplands. Golden eagles (Aquila chrysaetos) are long-lived raptors with high nest-site fidelity and relatively low reproductive success. Population trends of golden eagles in western United States are unclear although long-term monitoring of populations shows declines in occupancy and breeding performance. Northern harriers (Circus cyaneus) prefer relatively open grasslands and …

Improving Feed Efficiency Through Forage Strategies For Increasing Dairy Profitability And Sustainability, Jon Patrick Pretz

Electronic Theses and Dissertations

Three studies were conducted to determine production parameters and study specific hypothesis in regard to improving feed efficiency through various forage utilization strategies with or without the inclusion of various supplemented products. The first study evaluated the supplementation of a cobalt-lactate product and its effects on fiber digestibility and milk production parameters when fed to cows consuming a 70% forage diet. Treatments included: 1) CONTROL diet containing 12.5 mg/cow/d of cobalt (carbonate carbonate) and 2) TEST diet being the same basal diet but including an additional 50 mg/cow/d of cobalt via a 1% Co-lactate product (Co-Max®). In a feeding trial …

Effects Of Bran Treatment On Rheology And Sensory Quality Of Whole Wheat Flat Bread, Jigyasha Mishra

Electronic Theses and Dissertations

The purpose of this research was to study the differences in the quality of 100% whole wheat flour (WWF) when the bran fraction was subjected to mild solvent treatment before reconstitution with the endosperm. The bran treatments included water washing and washing with ethanol solutions (50% and 100%). WWF prepared with untreated bran served as the control. Effects of these treatments on particle size, chemical composition, color, dough rheology, product formulation, and sensory characteristics of 100% whole wheat flour were studied. Grains of five HRSW cultivars (Advance, Prevail, Select, Brick and Forefront) were milled using a Quadrumat Senior mill. Gluten …

A Qualitative Analysis Of Barriers To And Facilitators Of Successful Weight Loss, Alyson Drooger

Electronic Theses and Dissertations

Background: Along with the United States obesity epidemic comes extensive weight loss attempts. One way people are attempting to lose weight is through meal replacement programs. Much work has been done to study strategies of structured weight loss programs and examine their success. Limited work has been done to study the specific barriers and facilitators of the real life participants who join weight loss programs. The purpose of this study is to identify, through qualitative research methods, the barriers to and facilitators of weight loss while participating in a meal replacement program.

Methods: Twenty-nine members of a meal replacement program …

Temporal And Spatial Influences On South Dakota Farm Economies Of Size, Henry Brown

Electronic Theses and Dissertations

Census of Agriculture data are evaluated to test for economies of size of South Dakota farms. Economies of size occur when per-unit costs decrease as the size of a firm increases. This research measures technical economies of size, which assume that all firms pay identical input prices and receive identical output prices. The dependent variable of the model is expenses per dollar of sales. This is a typical measure of efficiency in agricultural statistics. The data come from the four prior Census of Agriculture years, 1997, 2002, 2007, and 2012. A state-wide model and a county-wide model were developed to …

Icook 4-H: Report Of Accelerometer Derived Physical Activity In 9-10 Year Old Children From Baseline, Post, And Follow-Up, Chase Merfeld

Electronic Theses and Dissertations

To examine the relationship between baseline, post, and follow-up accelerometer derived physical activity (PA) in 9-10 year old children participating in iCook 4-H, a 16-week, 5- state, randomized control family centered childhood obesity prevention program. This family focus intervention was designed to promote PA through lifestyle modifications. Between group differences in minutes-per-day and intensity of PA were determined on accelerometer compliant participants. All subjects wore an accelerometer (Actigraph GT3X+) for 7 days at baseline, post, and follow-up intervention assessment. Mean daily minutes of accelerometer derived sedentary time (ST) and PA including light (LPA), moderate (MPA), vigorous (VPA) and moderate-to-vigorous (MVPA) …

The Effect Of Copper And Zinc Source On Pre-Weaning Performance Of Cows, Health And Performance Of Suckling Calves, And Post-Weaning Feedlot Performance, Carcass Composition, And Meat Quality Of Calves, Jesse Fulton

Electronic Theses and Dissertations

During gestation an elevated level of nutrient intake is required by the cow in order to meet the requirements of the maturing fetus and if these requirements are not met, changes in fetal development can occur. However, little is understood about the effects of gestational manipulation of specific nutrients, such as minerals, on progeny growth, performance and carcass traits. Previous research supports the hypothesis that minerals provided in an organic form should be more soluble and readily available to the body for absorption. Therefore the objectives of this study were to determine if source of gestational and post-natal Cu and …

Three Overlooked Issues: Water Quality, Forage Quality, And Forage Blends Impacting The Feed Efficiency Of Dairy Cows, Ishwary Prasad Acharya

Electronic Theses and Dissertations

The overall objectives of the six studies were to increase the feed efficiency in lactating dairy cows. The first study evaluated the efficacy of different water sources and treatment systems on ruminal parameters using an in vitro gas production system. Outcomes of the study suggested that the source and nutrient quality of water can affect rate of ruminal fermentation. Thus, use of real farm water being offered to the cow to conduct in vitro gas production measurements may accurately predict the ruminal fermentation, digestibility and total gas production. The second study evaluated two recently developed leafy-floury corn silage hybrids against …

Manufacture, Concentration, And Functionality Of Micellar Casein Concentrate, Dustin Grossbier

Electronic Theses and Dissertations

This research has been structured into 3 major parts. The first part evaluates a centrifugal evaporator to concentrate micellar casein concentrate (MCC). The second part evaluates a wiped film evaporator (WFE) to concentrate MCC. The third part evaluates heat stability of the high concentration MCC produced by WFE. In part 1, the primary objective was to achieve >25% total solids (TS) without a loss of water removal rate associated with high solids materials. This is of importance, industrially, as this could reduce processing costs or eliminate drying all together. The major finding was that it did not offer any benefits …

Study Of The Effect Of Feeding Highly Digestible Corn Based Calf Starter On The Growth Performance Of Calves, Shruti Srivastava

Electronic Theses and Dissertations

New corn hybrids have been developed by Masters Choice (MC) that vary in energy density and starch digestibility. These MC hybrids have lower starch densities due to an altered starch structure, which allows for greater ruminal and intestinal starch digestion. The altered starch structure of these new hybrids results in reduced feed manufacturing costs (i.e., grinding). Thirty (30) 1 to 3 d old (40.6 ± 1.72 kg) Holstein heifer calves were randomly assigned to 1 of 2 calf starters to measure growth performance of Holstein heifer calves through 8 weeks of age. Treatments were: 1) Hard starch (HS) corn calf …

Changes In Wetland Conditions And Wetland Plant Communities In The Prairie Pothole Region After 50 Years, Ryann Cressey

Electronic Theses and Dissertations

Wetlands in Stutsman County, North Dakota were revisited after 50 years to assess changes in wetland conditions and plant communities within wetland zones in the Prairie Pothole Region. In 1961-1966, Robert E. Stewart and Harold A. Kantrud conducted a study to investigate the relationship of wetland plant communities to variations of water permanence and chemistry on three study areas: Crystal Springs, Cottonwood, and Mt. Moriah. Within in this region, a severe drought occurred in 1988- 1992 followed by the longest deluge starting in 1993 with wetlands still impacted today. In 2013 and 2014, I revisited 80 of the original wetlands …

Colony Stimulating Factor-1 Receptor (Csf-1r) Expression, Degradation, And Signaling In Lipid-Laden Macrophages, Gregory Thompson

Electronic Theses and Dissertations

Atherosclerosis is a serious disease affecting a large portion of the world’s population. In atherosclerosis, macrophages become filled with lipid droplets and form fatty plaques in artery walls. The formation of these atherosclerotic plaques is dependent on the macrophage growth factor colony stimulating factor-1 (CSF-1); however, understanding the changes in CSF-1 signaling and related pathways in foam cells remain poorly investigated. Lipid-laden macrophages were formed through exposure of murine bone marrow derived macrophages to 100 μg/ml acetylated LDL for 24 h. Non-LDL treated macrophages were grown in parallel for each experiment and used for comparison. Immunofluorescent staining was used to …

Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah

Electronic Theses and Dissertations

The arbuscular mycorrhizal (AM) symbiosis is arguably the world’s most abundant and important mutualism, and brings together the roots of the majority of land plants and AM fungi to great mutual advantage. The AM symbiosis can increase the uptake of nutrients, such as phosphorus (P) and nitrogen (N), and improves the abiotic and biotic stress resistance of the host plant. AM fungi have the potential to act as biofertilizers and bioprotectors in sustainable agriculture. However, despite its significance, the mechanisms that control the resource exchange between both partners in the arbuscular mycorrhizal symbiosis are largely unknown. The main aim of …

Evaluation Of Flame Weeding In Southeastern South Dakota, Benjamin William Arlt

Electronic Theses and Dissertations

Weed management is the main yield-limiting factor in production of organic crops. Currently, organic producers have limited resources available for in-row weed management. Flame weeding has been recognized for its potential as one part of an integrated approach at weed management in organic systems. Flame weeding uses propane fueled torches to heat plant tissue and denature proteins, thus injuring or destroying the affected tissue. When properly timed, flame weeding can effectively be used for weed control in corn and soybean fields. Corn and soybean have both shown tolerances to flame weeding and minimal yield loss with well-managed and timed flame …

Immune And Endocrine Modulations Associated With Enhanced Plant-Based Protein Diets Within Freshwater Fish Species, Timothy J. Bruce

Electronic Theses and Dissertations

The incorporation of bioprocessed soybean meals (BSBMs) as a protein replacement for marine-derived fish meal (MDFM) has gained momentum with the potential for aquafeed cost reduction, improved growth performance, and reducing exploitation on wild fish stocks. Further, potential health benefits and optimized production performance resulting from bioprocessed feed ingredients have led to research on the nutraceutical benefits of plant-based ingredients. A novel proprietary MBBM has been developed using a microbial conversion of carbohydrates into digestible protein and additional cellular components that have immunogenic potential. A series of five finfish feeding trials were conducted to investigate immunomodulation induced by the BSBM …

Corncob Grit Application As An Alternative To Control Weeds In Two Crop Production Systems, Mauricio Erazo-Barradas

Electronic Theses and Dissertations

Weed management is one of the most challenging production problems in organic cropping systems because of limited weed control methods. Grits, derived from agricultural residues, have been demonstrated to control weed seedlings selectively in corn. This research examined weed efficacy and crop yield of an integrated air-propelled abrasive corncob grit (for in-row weed control) at varying timings and frequencies and flame-weeding or cultivation (for between-row weed control) system in two corn production systems. In the first study efficacy of weed control was assessed with this approach in an organic corn silage production system established in Morris, MN in 2013 and …

The Effects Of Preovulatory Estradiol On The Uterine Environment And Conceptus Survival From Fertilization To Maternal Recognition Of Pregnancy, Emmalee J. Northrop

Electronic Theses and Dissertations

Preovulatory estradiol has been reported to play a critical role in pregnancy establishment and embryonic survival, but the mechanism by which estradiol exerts its effects has not been well characterized. The objective of this thesis is to determine the effects of preovulatory estradiol on the uterine environment and conceptus survival from fertilization to maternal recognition of pregnancy. Beef cows/heifers were synchronized with the CO-Synch protocol and AIed (d 0). Cows were classified by estrus expression (estrus and no estrus), and uteri were flushed to collect d16 conceptuses nonsurgically (Rep 1; n = 29), or following slaughter (Rep 2; n = …

Effects Of Small Intestinal Starch Digestion And Dietary Lipid On Efficiency Of Nitrogen Use In Cattle, Ethan J. Blom

Electronic Theses and Dissertations

The objective of this research was to determine the effects of increases in energy available for gain from increased small intestinal starch digestion (SISD) and dietary lipid source and amount on the efficiency of N use in cattle. Five ruminally, duodenally, and ileally cannulated steers were placed in a 5 × 5 Latin square. Each received duodenal infusion of 1.5 ± 0.08 kg/d raw cornstarch and either 0, 30.9 ± 0.59, 62.4 ± 1.16, or 120.4 ± 3.39 g/d Glu, or 387.9 ± 17.47 g/d casein. Casein increased (P = 0.05) SISD. Similarly, greater duodenal Glu linearly (P = 0.02) …

Attitude Comparison Among South Dakota Family Members Regarding Estate Planning And Farm Transitions, Heather Gessner

Electronic Theses and Dissertations

Estate planning and intergenerational farm transitions are the focus of research and extension programs across the nation due to the potential tax consequences faced by farm operations, retirement funding options available and utilized by farmers, changes in asset transition tools available, and communication (or lack of communication) issues related to bringing an heir back to the family operation.
Past plans for research projects and extension programming developed across the nation and in South Dakota (SD), were created with many assumptions regarding the attitudes of farm family members towards the transition of ownership of the operational assets and personal assets of …