Life Sciences Commons^™

Growing South Dakota (Winter 2024), Michelle Cartney

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Continuing a Leadership Journey
[Page] 5 CAFES Leadership Team Grows
[Page] 7 Award-Winning Faculty & Staff
[Page] 9 New Endowment Holders Recognized
[Page] 10 Inaugural Precision Livestock Field School Offered at SDSU Cottonwood Field Station
[Page] 11 SDSU Celebrates Professional Program in Veterinary Medicine Students [Page] 13 Clay Carlson, SDSU Meat Science Graduate Student Approaches School and Life with Winning Mindset
[Page] 15 SDSU Alumni has been selected as the Head Coach of the South Dakota State University Livestock Judging Team
[Page] 16 Livestock Units Promote Hands-On Learning & Premier Research
[Page] 20 SDSU leads NSF-backed fertilizer development …

Growing South Dakota (Fall 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Back-to-Back Champions: SDSU Quarter Scale Tractor Team Repeats International Win
[Page] 6 CAFES Welcomes New Members to College Leadership Team
[Page] 9 Vikram Mistry Retires After Nearly 40-Year Career at SDSU
[Page] 12 Bill Gibbons Retires After 36 Years at SDSU
[Page] 13 Nic Uilk: Klingbeil Endowed Educator in Precision Agriculture
[Page] 15 SDSU Students Study Abroad
[Page] 19 CAFES Faculty Claim National Awards
[Page] 21 Dairy Alum Sanne de Bruijn Grabs Global Opportunities
[Page] 23 SDSU Extension Cohort Programs
[Page] 25 Summer Field Day Highlights
[Page] 29 Jackrabbits Now and Then

Growing South Dakota (Summer 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

3 SDSU Little International Celebrates 100 years of Tradition
7 2023 CAFES Celebration of Faculty Excellence
11 South Dakota Agricultural Experiment Station Locations and Research Initiatives
15 Every Acre Counts
16 Undergraduate Research
19 Tanner Sloan: South Dakota State Wrestler & Animal Science Student Takes Home NCAA Division I & U23 World Silver Medals
21 2023 CAFES Outstanding Seniors
23 Robert Streeter: International Advocate for Wildlife Conservation
25 Collegiate Cattlemen's Club
27 Jim and Melody Mielke: Lifelong Donors Contribute to Agriculture in More Ways than One
29 Jackrabbits Now and Then

Perspectives Of Dairy Employees At The Beginning Of The Covid-19 Pandemic: A Survey Of Health Risks And Educational Needs, Fernanda Ferreira, Maristela Rovai, Mireille Chahine, Mario E. De Haro-Martí, John Wenz, Joseph Dalton, Noelia Silva-Del-Rio

Dairy Science and Food Science Faculty Publications: 2022 to Present

Our objective was to understand dairy employees' perceptions and educational needs at the onset of the COVID-19 pandemic. A bilingual (English and Spanish), anonymous survey targeted at dairy employees was circulated nationwide via university and allied industry media outlets. Responses (n = 63) from 11 states were received (May–Sep. 2020). Respondents worked in herds ranging from 50 to 40,000 animals in size. Dairy managers (33%) responded mostly to the English survey (52%), whereas entry-level workers (67%) chose the Spanish format (76%). Survey results highlighted different perspectives, educational needs, and preferred sources of information between English- and Spanish-speaking dairy workers. Overall, …

Growing South Dakota (Spring 2023), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 Developing Climate-Smart Beef and Bison Commodities
[Page] 5 Joseph Cassady Leads CAFES as New Dean
[Page] 7 Inaugural Class of Klingbeil Scholars
[Page] 9 Jackrabbits to Future Veterinarians
[Page] 11 Protecting South Dakota’s Grasslands
[Page] 13 Growing Youth Programs
[Page] 15 Partnerships for Positive Growth
[Page] 17 Building Highly Effective Boards
[Page] 19 SDSU Extension Podcasts
[Page] 21 Providing Good Food for All
[Page] 23 Improving Seed Varieties
[Page] 25 CAFES Endowed Positions
[Page] 29 Jackrabbits Now and Then

2d Respiratory Sound Analysis To Detect Lung Abnormalities, Rafia Sharmin Alice, Kc Santosh

SDSU Data Science Symposium

Abstract. In this paper, we analyze deep visual features from 2D data representation(s) of the respiratory sound to detect evidence of lung abnormalities. The primary motivation behind this is that visual cues are more important in decision-making than raw data (lung sound). Early detection and prompt treatments are essential for any future possible respiratory disorders, and respiratory sound is proven to be one of the biomarkers. In contrast to state-of-the-art approaches, we aim at understanding/analyzing visual features using our Convolutional Neural Networks (CNN) tailored Deep Learning Models, where we consider all possible 2D data such as Spectrogram, Mel-frequency Cepstral Coefficients …

Whole Genome Sequencing For Identification Of Putative Causative Alleles For Hemochromatosis In Horses, Anna M. Rauber-Ramos

Schultz-Werth Award Papers

Hemochromatosis is liver disorder in which excess iron is stored in the liver, leading to iron overload and extensive liver damage that can prove fatal. Based on human precedence, a genetic cause for idiopathic hemochromatosis in horses is suspected. Two horses with hemochromatosis were whole genome sequenced, with variants in candidate genes prioritized in analyses of potential pathogenicity. No clear putative variants were found to be present in both horses, suggesting an etiology more complex than the initially suspected Mendelian inheritance. A heterozygous nonsense mutation in STEAP3 metalloreductase (STEAP3) was identified as a potential contributor to hemochromatosis in one of …

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Effects Of Anthocyanin Supplementation In Diet On Glycemic And Related Cardiovascular Biomarkers In Patients With Type 2 Diabetes: A Systematic Review And Meta-Analysis Of Randomized Controlled Trials, Ting Mao, Fnu Akshit, Maneesha S. Mohan

Dairy Science and Food Science Faculty Publications: 2022 to Present

Purpose: This study is the first systematic review and meta-analysis based on RCTs on the effects of anthocyanins on patients with type 2 diabetes mellitus (T2DM) and the effect on T2DM-related cardiovascular disease. Methods: RCTs published in English from five electronic databases were evaluated for glycated hemoglobin (HbA1c), fasting blood glucose (FBG), 2-h postprandial blood glucose, fasting insulin, model assessment for insulin resistance, triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, systolic blood pressure, and diastolic blood pressure. The quality of the studies was rated (Cochrane Risk of Bias tool) and weighted mean differences were …

Teaching Central Dogma Of Biology Using Cell Free Environment, Lukas Nelson, Sudeshna Roy, Bobbi J. Bohnet, Tracy Chase, Brian Labelle, Nicholas Butzin, Madhav P. Nepal

iLEARN Teaching Resources

In this lesson plan, students will learn and visualize how genetic information coded in DNA is transcribed into RNA which is further translated into protein. This entire process, DNA->RNA-> Protein, is known as the Central Dogma of Molecular Biology. This hands-on module is designed for 9^th through 12^th grade students. To adjust the difficulty level of the modules, some aspects can be modified or removed based on the grade. This module includes an instructional slide show, video tutorial, assessment options, and links to additional resources.

Teaching Dna Extractions From Fruits (Strawberry, Banana, Kiwi, And Watermelon), Amadea Pourier, Dillon Nelson, Wiyaka Pomarleau, Kay Cutler, Madhav P. Nepal

iLEARN Teaching Resources

In this lesson, students will use a simple DNA extraction solution and isopropyl alcohol to extract DNA from strawberries, banana, kiwi, and watermelon. The extraction solution consists of dish detergent, water, and table salt. Grinding or crushing the soft fruit tissue will separate the cells. The dish detergent breaks down the membranes, exposing the DNA to the solution. The salt helps the DNA molecule to stick together from the proteins. The cold alcohol dissolves everything but the DNA, which forms an insoluble layer of the DNA allowing it to be visible.

Growing South Dakota (Fall 2021 / Winter 2022), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 SDSU Launches New Professional Program in Veterinary Medicine
[Page] 7 The 6th Generation
[Page] 9 First-Gen Proud
[Page] 11 Student Success
[Page] 21 Alumni News
[Page] 27 CAFES News and Updates
[Page] 45 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal

Electronic Theses and Dissertations

In this study, the role of IFITM3 on PRRSV replication was studied in vitro by expressing exogenous IFITM3 in MARC-145 cells. An average of 31% reduction in PRRSV N protein expression and an average of 5.4 fold decrease in virus titer in the supernatant were observed in IFITM3 overexpressing cells as compared to vector control cells at 24 hours post infection (hpi). Moreover, there was a positive correlation between interferon- induced IFITM3 up-regulation and reduced PRRSV replication. To determine the role of endogenous IFITM3 in PRRSV replication, siRNA induced knockdown of IFITM3 was employed. RT-PCR validated the successful silencing of …

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …

Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain

Electronic Theses and Dissertations

This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …

Growing South Dakota (Spring 2021), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the 2020 SDSU Extension Annual Report and 2020 South Dakota Agricultural Experiment Station Annual Report.
[Page] 3 COVID-19 Impacts
[Page] 9 Precision Agriculture: What's New
[Page] 13 Featured Research
[Page] 19 SDSU Extension 2020 Annual Report
[Page] 45 South Dakota Agricultural Experiment Station 2020 Annual Report
[Page] 55 CAFES News and Updates
[Page] 67 Alumni News
[Page] 75 Alumni Gone Down in History
[Page] 81 Jackrabbits Now and Then: A Current Student and Alumni Q&A

An Analysis Of Suicide Risk Factors Among Farmers In The Midwestern United States, Andrea Bjornestad, Courtney Cuthbertson, Jessie Hendricks

Counseling and Human Development Faculty Publications

Research on the complex relationships of variables contributing to farmer suicide is limited. The purpose of the study was to examine factors associated with suicide risk through the use of standardized instruments measuring psychological (depression, anxiety), social (social support), and contextual factors. A questionnaire was completed by 600 farmers in the Midwestern United States. A multiple linear regression model was used to analyze associations with suicide risk (SBQ-R), including depression (PHQ-9), anxiety (GAD-7), Brief COPE subscales (BC), social support (MSPSS), and select demographic and farming characteristics. The only variable that emerged as having a significant relationship with the natural log-transformed …

Health & Nutritional Sciences Free Communication Day: Spring 2021 Plan B Abstracts, Brianna Vig, Michael Pappan, Taylor Svehla, Alexander Iverson, Tanner Jerome, Christian Gravius, Kaitlyn Jackman, Savannah Ratermann, Gwyneth Phillips, Jenna Rheder, Erin Hallenbeck, Logan Ammons, Rachel Astleford, Francine Hoffman, Ryan Folley, Kylie Serie, Jenna Christianson, Haley Strait, Kelsey Kessler

Health and Nutritional Sciences Graduate Students Plan B Capstone Projects

This document contains Plan B abstracts presented by graduate students at the Health and Nutritional Sciences Free Communication Day, held on May 4th, 2021.

Abstract titles include:

• Local Restaurants’ Marketing and Sponsorship Within Collegiate Athletics
• Preparing the Future of Campus Recreation
• Motivational Factors Influencing College Choice: NCAA Division I Female Volleyball Athletes
• The Significance of a Successful Internship Program
• Foundations of Publicly Subsidized Sport Stadiums: The Case of U.S. Bank Stadium
• Enhancing Member Experience During a Pandemic
• The Risk of Depression in Football Players Diagnosed with a Concussion
• Effectiveness of Color-Tinted Glasses in Reducing Photophobia in Patients Diagnosed with Mild …

Cortisol In Hair As A Measure Of Chronic Stress During Sow Gestation And The Pattern Of Cortisol In Blood During Parturition In Sows, Talia Everding

Electronic Theses and Dissertations

Cortisol is known as the stress hormone, as it influences many metabolic processes to maintain glucose homeostasis during stressful experiences, including physical and psychological stress. It can be detected in biological matrices such as blood and hair and is released rapidly during sudden stressors and continuously during longterm stress. Blood cortisol fluctuates rapidly in response to acute stressors like pain, exertion, and fear; in hair cortisol accumulates steadily over the period of hair growth and may be useful for detecting chronically elevated cortisol resulting from long-term stress. The objective of this research was to, 1) determine the influence of a …

Characterization Of Cucurbitacin-Inspired Estrone Analogues As Novel Inhibitors Of Human Atp- Binding Cassette Proteins (Abcb1 And Abcc1), Jennifer Kyeremateng

Electronic Theses and Dissertations

ATP-binding cassette (ABC) transporters are a large class of integral membrane proteins that contribute to key physiological functions in all organisms by utilizing ATP binding and hydrolysis to transport diverse substrates across membrane barriers. P-glycoprotein (P-gp/ ABCB1) and Multidrug Resistance protein 1 (MRP1/ABCC1) are widely reported ABC transporters associated with multidrug resistance in cancer. Multidrug resistance (MDR) mediated by P-gp and MRP1 is responsible for treatment failures of many metastatic cancers as a result of reduced accumulation, bioavailability and diminished potency of anticancer drugs. Currently, known P-gp and MRP1 inhibitors are limited due to toxicity, lack of selectivity and low …

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …

South Dakota State University : Research 2020, Division Of Research And Economic Development

Research: South Dakota State University

[Page] 2 New respirator design to capture, kill coronavirus
[Page] 3 SDSU scientists to examine how coronavirus infects cells
[Page] 4 State diagnostic lab fulfills need for human COVID-19 testing
[Page] 5 Isaacson to help develop tribal palliative care programs
[Page] 6 Sun Grant funding fuels bioprocessing research
[Page] 8 Prairie AquaTech exporting high-protein feed ingredient
[Page] 11 New connection makes building repair fast, cost-effective
[Page] 12 Record-setting wildfire season drastically increases emissions
[Page] 14 Engineering study examines sunflower stem growth
[Page] 15 State Poet Laureate unveils ‘South Dakota in Poems'
[Page] 16 Reineke receives NIH grant to help eradicate …

Growing South Dakota (Spring/Summer 2020), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the South Dakota Agricultural Experiment Station 2019 Annual Report.

[Page] 2 South Dakota Agricultural Experiment Station Update
[Page] 4 Featured Research
[Page] 14 Graduate and Undergraduate Research: In Our Own Words
[Page] 20 Featured Research
[Page] 32 Research in Progress
[Page] 38 CAFES News and Updates
[Page] 56 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Growing South Dakota (Summer 2020), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 3 COVID-19: From Our Students' Perspective
[Page] 11 Class of 2020 Q&A
[Page] 19 Alumni Advice for the Future Generation
[Page] 21 Recent Alumni Features
[Page] 25 Student Features
[Page] 31 CAFES News, Student Success, Awards and Achievements
[Page] 57 Jackrabbits Now and Then: A Current Student and Alumni Q&A

Development And Diagnostic Application Of Monoclonal Antibodies Against Swine Acute Diarrhea Syndrome Coronavirus (Sads-Cov), Molly Kroeger

Schultz-Werth Award Papers

Swine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) is a member of the Coronaviridae family. The virus is associated with severe small intestine inflammation and diarrhea in suckling piglets. In 2017, SADS-CoV was first detected and identified as the causative agent of a devasting swine disease outbreak in southern China. Routine monitoring and early detection of the source of infection is therefore integral to the prevention and control of a SADS-CoV outbreak in the United States. However, the United States does not currently have any diagnostic or surveillance tests to identify this emerging disease. To address these industry needs, we developed monoclonal …

The Journal Of Undergraduate Research: 2019

The Journal of Undergraduate Research

This is the complete issue of the South Dakota State University Journal of Undergraduate Research, Volume 17.

Growing South Dakota (Winter 2020), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

This issue contains the SDSU Extension 2019 Annual Report

[Page] 2-3 South Dakota Animal Disease Research and Diagnostic Laboratory Ribbon Cutting Ceremony
[Page] 4 SDSU Extension
[Page] 4 From the Director of Extension Karla Trautman
[Page] 5 2019 USDA Risk Management Agency Crop Indemnities
[Page] 6-8 SDSU Extension Responds to Mother Nature in 2019
[Page] 9 Successful Energize! Conference Buoys Community Efforts in Lemmon & Beyond
[Page] 10-11 Helping Loved Ones Transition Smoothly
[Page] 12 AmeriCorps VISTA Members Expand Extension Programming
[Page] 13 South Dakota Wellness Coalitions Make Big Impacts in Small Communities [Page] 14-16 Extension Programs in Our Own …

Antidepressant Effects On Rem Sleep, Mitchel Adams

Biology and Microbiology Graduate Students Plan B Research Projects

Individuals who deal with depression are typically prescribed antidepressants in order to alleviate the depressive symptoms that they may be having. Polysomnographic sleep research has revealed that depression is associated with altered sleep architecture and distorted REM sleep quality.² Specifically, increased REM sleep duration and density have proved to be markers that predict recurrence and relapse for depressed individuals. REM sleep serves to stimulate regions of the brain that are important with learning and memory consolidation. However, an increased duration of REM sleep in depressed individuals proves to be unfavorable. Antidepressants drugs aim to weaken sleep quality, which is …

Freestanding Emergency Departments: A Possible Solution To Rural Hospital Closure, Emily Steadman

Biology and Microbiology Graduate Students Plan B Research Projects

Freestanding emergency departments have become more and more common over the years and have started to pop up in many states across the country. A freestanding emergency department is a facility that provides healthcare in the form of emergency services separate from those at a hospital. FEDs can be seen as independent structures that are distinct from hospital-based emergency rooms and urgent facilities. Just as freestanding emergency departments have spread across the country in a bit of a frenzy, another prevalent issue has been sweeping the country, the closure of rural hospitals. Over the last decade rural hospitals have struggled …

A Social Review Of The Opioid Epidemic: The History, Pathophysiology, And Effects On Rural America, Holly J. Goehring

Biology and Microbiology Graduate Students Plan B Research Projects

One of the more pressing issues in modern day health care is the nationwide heath care crisis known as the opioid epidemic. The beginning of this literature review will focus on the history of opioid use and early prescription rates, as well as the summary and definitions of the drugs involved in the current opioid epidemic. The review then shifts to the pathophysiology of addiction involving the activation of opioid receptors and opioid use disorder. The main focus of this literature review is the societal impacts of the opioid epidemic, making special reference to the economic burden and effects on …