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Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Biological Sciences: Faculty Publications
A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent …