Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Agouti (1)
- Catalytic RNA (1)
- Coastal forests (1)
- Cytoplasmic streaming (1)
- Deer mice (1)
-
- Estuaries (1)
- Hurricanes (1)
- Immunofluorescence (1)
- In vitro selection (1)
- Land Margin Ecosystems Research (1)
- Myosin (1)
- Nuclear migration (1)
- Nucleic acids (1)
- Peromyscus maniculatus (1)
- Recovery from disturbance (1)
- Self-organization (1)
- Storm effects (1)
- Transport stress (1)
- Waquoit Bay (1)
- Western blotting (1)
Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Ecological Effects Of Major Storms On Coastal Watersheds And Coastal Waters: Hurricane Bob On Cape Cod, I. Valiela, P. Peckol, C. D'Avanzo, J. Kremer, D. Hersh, K. Foreman, K. Lajtha, B. Seely, W. R. Geyer, T. Isaji, R. Crawford
Ecological Effects Of Major Storms On Coastal Watersheds And Coastal Waters: Hurricane Bob On Cape Cod, I. Valiela, P. Peckol, C. D'Avanzo, J. Kremer, D. Hersh, K. Foreman, K. Lajtha, B. Seely, W. R. Geyer, T. Isaji, R. Crawford
Biological Sciences: Faculty Publications
Hurricane Bob, a category 3 storm, made landfall on Cape Cod in August 1991, and its effects on watersheds and adjoining estuaries were detected in the ongoing studies being caried out as part of the Waquoit Bay Land Margin Ecosystems Research project. On land, Bob had only minor overall effects on forests; localized wind bursts did snap and break trees in small and widely scattered forest parcels. Wind stripped up to half the leaves of deciduous trees and many herbaceous plants on the watershed, and most remaining leaves were damaged by salt, so that by the end of Aug, Cape …
Thermoregulation Of Escherichia Coil Pap Transcription: H-Ns Is A Temperature-Dependent Dna Methylation Blocking Factor, Christine A. White-Ziegler, Melinda L. Angus Hill, Bruce A. Braaten, Marjan W. Van Der Woude, David A. Low
Thermoregulation Of Escherichia Coil Pap Transcription: H-Ns Is A Temperature-Dependent Dna Methylation Blocking Factor, Christine A. White-Ziegler, Melinda L. Angus Hill, Bruce A. Braaten, Marjan W. Van Der Woude, David A. Low
Biological Sciences: Faculty Publications
The expression of Pap pili that facilitate the attach- ment of Escherichia coli to uroepithelial cells is shut off outside the host at temperatures below 268C. Ribo- nuclease protection analysis showed that this thermo- regulatory response was rapid as evidenced by the absence of papBA transcripts, coding for Pap pilin, after only one generation of growth at 238C. The his- tone-like nucleoid structuring protein H-NS and DNA sequences within papB were required for thermoregu- lation, but the PapB and PapI regulatory proteins were not. In vivo analysis of pap DNA methylation patterns indicated that H-NS or a factor regulated by …
The Immunodominant Brugia Malayi Paramyosin As A Marker Of Current Infection With Wuchereria Bancrofti Adult Worms, Sandra Langy, Catherine Plichart, Patrick Luquiaud, Steven A. Williams, Luc Nicolas
The Immunodominant Brugia Malayi Paramyosin As A Marker Of Current Infection With Wuchereria Bancrofti Adult Worms, Sandra Langy, Catherine Plichart, Patrick Luquiaud, Steven A. Williams, Luc Nicolas
Biological Sciences: Faculty Publications
The full-length cDNA sequence encoding Brugia malayi L3 paramyosin has been isolated by immunoscreening a cDNA library with a mouse antiserum raised against Wuchereria bancrofti L3 infective larvae. A recombinant truncated form of paramyosin was expressed as a glutathione S-transferase fusion protein and used to evaluate humoral responses of adults from a W. bancrofti-endemic area in French Polynesia according to their parasitological status. Immunoglobulin G4 (IgG4) preferentially bound to paramyosin in W. bancrofti-parasitized individuals, in contrast to unparasitized individuals, who harbored neither microfilaria nor Og4C3 adult worm circulating antigen. Reduction of the anti-paramyosin IgG4 titer following combined chemotherapy with diethylcarbamazine …
Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Immunochemical And Immunocytochemical Identification Of A Myosin Heavy Chain Polypeptide In Nicotiana Pollen Tubes, X. J. Tang, P. K. Hepler, Stylianos P. Scordilis
Biological Sciences: Faculty Publications
A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent …
Experimental Evolution Of Complexity: In Vitro Emergence Of Intermolecular Ribozyme Interactions, Martin M. Hanczyc, Robert L. Dorit
Experimental Evolution Of Complexity: In Vitro Emergence Of Intermolecular Ribozyme Interactions, Martin M. Hanczyc, Robert L. Dorit
Biological Sciences: Faculty Publications
In the course of evolving variants of the Tetrahymena thermophila Group I ribozyme for improved DNA cleavage in vitro, we witnessed the unexpected emergence of a derived molecular species, capable of acting as a partner for the ribozyme, but no longer autocatalytic. This new RNA species exhibits a deletion in the catalytic core and participates in a productive intermolecular interaction with an active ribozyme, thus insuring its survival in the population. These novel RNA molecules have evolved a precise catalytic interaction with the Group I ribozyme and depend for their survival on the continued presence of active catalysts. This interaction …
Nodule Organogenesis And Symbiotic Mutants Of The Model Legume Lotus Japonicus, Krzysztof Szczyglowski, Robert S. Shaw, Judith Wopereis, Sue Copeland, Dirk Hamburger, Beth Kasiborski, Frank B. Dazzo, Frans J. De Bruijn
Nodule Organogenesis And Symbiotic Mutants Of The Model Legume Lotus Japonicus, Krzysztof Szczyglowski, Robert S. Shaw, Judith Wopereis, Sue Copeland, Dirk Hamburger, Beth Kasiborski, Frank B. Dazzo, Frans J. De Bruijn
Biological Sciences: Faculty Publications
A detailed microscopical analysis of the morphological features that distinguish different developmental stages of nodule organogenesis in wild-type Lotus japonicus ecotype Gifu B-129-S9 plants was performed, to provide the necessary framework for the evaluation of altered phenotypes of L. japonicus symbiotic mutants. Subsequently, chemical ethyl methanesulfonate (EMS) mutagenesis of L. japonicus was carried out. The analysis of approximately 3,000 M1 plants and their progeny yielded 20 stable L. japonicus symbiotic variants, consisting of at least 14 different symbiosis- associated loci or complementation groups. Moreover, a mutation affecting L. japonicus root development was identified that also conferred a hypernodulation response when …
Detection Of The Filarial Parasite Mansonella Streptocerca In Skin Biopsies By A Nested Polymerase Chain Reaction-Based Assay, Peter U. Fischer, Dietrich W. Büttner, Jotham Bamuhiiga, Steven A. Williams
Detection Of The Filarial Parasite Mansonella Streptocerca In Skin Biopsies By A Nested Polymerase Chain Reaction-Based Assay, Peter U. Fischer, Dietrich W. Büttner, Jotham Bamuhiiga, Steven A. Williams
Biological Sciences: Faculty Publications
To differentiate the skin-dwelling filariae Mansonella streptocerca and Onchocerca volvulus, a nested polymerase chain reaction (PCR) assay was developed from small amounts of parasite material present in skin biopsies. One nonspecific and one specific pair of primers were used to amplify the 5S rDNA spacer region of M. streptocerca. Biopsies with different microfilaria densities obtained from 104 Ugandans living in an area endemic forM. streptocerca were tested using both the nested PCR assay and standard parasitologic assessment of microfilariae. All 82 samples from microfilaria carriers were positive when tested using the nested PCR assay. In addition, M. streptocerca DNA could …
Effect Of Transatlantic Transport On Reproduction Of Agouti And Nonagouti Deer Mice, Peromyscus Maniculatus, Virginia Hayssen
Effect Of Transatlantic Transport On Reproduction Of Agouti And Nonagouti Deer Mice, Peromyscus Maniculatus, Virginia Hayssen
Biological Sciences: Faculty Publications
In conjunction with establishing colonies of deer mice in the UK, effects of transportation on reproduction in agouti (A) and nonagouti (a) deer mice were assessed. Adults were shipped via ground courier and air freight from Northampton, Massachusetts, USA to Sutton Bonington, Leicestershire, England in February and June. Deer mice were paired upon arrival in Sutton Bonington, whereas matched controls were paired in the original colonies at shipping. To assess reproduction, the following variables were monitored for 110 days for all 96 pairs: number of pairs producing litters, time from pairing to birth, interlitter interval, litter size at birth, and …