Life Sciences Commons^™

Paramyosin Phosphorylation Site Disruption Affects Indirect Flight Muscle Stiffness And Power Generation In Drosophila Melanogaster, Hongjun Liu, Mark S. Miller, Douglas M. Swank, William A. Kronert, David W. Maughan, Sanford I. Bernstein

Mark S. Miller

The phosphoprotein paramyosin is a major structural component of invertebrate muscle thick filaments. To investigate the importance of paramyosin phosphorylation, we produced transgenic Drosophila melanogaster in which one, three, or four phosphorylatable serine residues in the N-terminal nonhelical domain were replaced by alanines. Depending on the residues mutated, transgenic lines were either unaffected or severely flight impaired. Flight-impaired strains had decreases in the most acidic paramyosin isoforms, with a corresponding increase in more basic isoforms. Surprisingly, ultrastructure of indirect flight muscle myofibrils was normal, indicating N-terminal phosphorylation is not important for myofibril assembly. However, mechanical studies of active indirect flight …

Effect Of Des-Aspartate-Angiotensin I On The Actions Of Angiotensin Ii In The Isolated Renal And Mesenteric Vasculature Of Hypertensive And Stz-Induced Diabetic Rats, Dharmani Devi A/P Murugan

Dharmani Devi A/P Murugan

The present study investigated the action of des-aspartate-angiotensin I (DAA-I) on the pressor action of angiotensin II in the renal and mesenteric vasculature of WKY, SHR and streptozotocin (STZ)-induced diabetic rats. Angiotensin II-induced a dose-dependent pressor response in the renal vasculature. Compared to the WKY, the pressor response was enhanced in the SHR and reduced in the STZ-induced diabetic rat. DAA-I attenuated the angiotensin II pressor action in renal vasculature of WKY and SHR. The attenuation was observed for DAA-I concentration as low as 10(-18) M and was more prominent in SHR. However, the ability of DAA-I to reduce angiotensin …

A New Species Of The Eleutherodactylus Nigrovittatus Group (Anura: Leptodactylidae) From Andean Peru, Edgar Lehr

Edgar Lehr

No abstract provided.

Cgkb-Cyanogroup Genomics Knowledge Base, Tin-Chun Chu, Lee Lee, Shankar Srinivasan, John Gaynor, Quinn Vega, Bonnie Lustigman

Tin-Chun Chu, Ph.D.

No abstract provided.

Pfcrt Is More Than The Plasmodium Falciparum Chloroquine Resistance Gene: A Functional And Evolutionary Perspective, Roland A. Cooper, Carmony L. Hartwig, Michael T. Ferdig

Roland A. Cooper

Historical Analysis And Impact Of Ken Dryden’S The Game, Don Morrow

Donald Morrow

No abstract provided.

Gender Differences In Lower Extremity Coupling Variability During An Unanticipated Cutting Maneuver, Joseph Hamill, Bryan C. Heiderscheit, Christine D. Pollard

Joseph Hamill

The purpose of this study was to determine if gender differences exist in the variability of various lower extremity (LE) segment and joint couplings during an unanticipated cutting maneuver. 3-D kinematics were collected on 24 college soccer players (12 M, 12 F) while each performed the cutting maneuver. The following intralimb couplings were studied: thigh rotation (rot)/leg rot; thigh abduction-adduction/leg abd-add; hip abd-add/knee rot; hip rot/knee abd-add; knee flexion-extension/knee rot; knee flx-ext/hip rot. A vector-coding technique applied to angle-angle plots was used to quantify the coordination of each coupling. The average between-trial standard deviation of the coordination pattern during the …

Quasistatic And Continuous Dynamic Characterization Of The Mechanical Properties Of Silk From The Cobweb Of The Black Widow Spider Latrodectus Hesperus, Todd A. Blackledge, John E. Swindeman, Cheryl Y. Hayashi

Todd A. Blackledge

Spider silks are among the strongest and toughest known materials, but investigation of these remarkable properties has been confined largely to orb-weaving spiders. We investigated the mechanical performance of silk from the cobweb-weaving spider Latrodectus hesperus. Both silk from the scaffolding region of the web and sticky gumfooted capture lines had material properties similar to the major ampullate silk that orb weavers use as the framework for their orb webs. Major ampullate fibers obtained from anaesthetized Latrodectus spiders were similar, but exhibited increased stiffness and reduced extensibility. Novel continuous dynamic analysis of the silks revealed that the loss tangent (tan …

High Speed Flight At Low Altitude - Hazard To Commercial Aviation?, Paul F. Eschenfelder

Paul F. Eschenfelder

No abstract provided.

A Multidimensional Approach For Detecting Species Patterns In Malagasy Vertebrates, Anne D. Yoder, Link E. Olson, Carol Hanley, Kellie L. Heckman, Rodin Rasoloarison, Amy L. Russell, Julie Ranivo, Voahangy Soarimalala, K. Praveen Karanth, Achille P. Raselimanana, Steven M. Goodman

Amy L. Russell

My Teaching Experience In Cambodia, Stephen Asma

Stephen T Asma

No abstract provided.

Effect Of Cadmium On The Growth Of Chlamydomonas, Suzen Awad, Tin-Chun Chu, Bonnie Lustigman, Lee Lee

Tin-Chun Chu, Ph.D.

No abstract provided.

A Literary Analysis Of Ken Dryden’S The Game.”, Don Morrow

Donald Morrow

No abstract provided.

Two Forms Of The Large Tumor Suppressor Gene (Lats1) Protein Expressed In The Vertebrate Retina, Novrouz B. Akhmedov, Clyde K. Yamashita, Dai Tran, Natik I. Piri, Gustavo D. Aguirre, Debora B. Farber

Gustavo D. Aguirre, VMD, PhD

The Importance Of Experimental Design In Proteomic Mass Spectrometry Experiments: Some Cautionary Tales, Jeffrey S. Morris, Jianhua Hu, Kevin R. Coombes, Keith A. Baggerly

Jeffrey S. Morris

Proteomic expression patterns derived from mass spectrometry have been put forward as potential biomarkers for the early diagnosis of cancer and other diseases. This approach has generated much excitement and has led to a large number of new experiments and vast amounts of new data. The data, derived at great expense, can have very little value if careful attention is not paid to the experimental design and analysis. Using examples from surfaceenhanced laser desorption/ionisation time-of-flight (SELDI-TOF) and matrix-assisted laser desorption–ionisation/time-of-flight (MALDI-TOF) experiments, we describe several experimental design issues that can corrupt a dataset. Fortunately, the problems we identify can be …

Qualidade Da Água Da Nascente De Anços (Serra De Sicó) Com O Uso De Fitoperifiton, Ana Sofia P.S. Reboleira

Ana Sofia P.S. Reboleira

O estudo diatomológico da nascente do Rio Anços (Serra de Sicó), foi efectuado sazonalmente, entre o Verão de 2002 e o Outono de 2004. Escolheu-se uma estação de colheita, na parede da nascente exposta à água que provém do interior do Maciço. Com o intuito de monitorizar a qualidade da água, foram recolhidas diatomáceas perifíticas. Condutividade, pH, temperatura e oxigénio dissolvido foram medidos na estação em cada colheita efectuada. Vinte e sete espécies, distribuídas por dezasseis géneros foram observados. Cocconeis placentula Ehrenberg var. lineata (Herenberg) Van Heurck foi a espécie dominante, seguida de Navicula minima Grunow, N. cryptotenella Lange-Bertalot, Gomphonema …

No Relationship Between Sequence Variation In Protein Coding Regions Of The Tas1r3 Gene And Saccharin Preference In Rats., Clinton Chapman, Ke Lu, Amanda Mcdaniel, Michael Tordoff, Li Xia, Gary Beauchamp, Nancy Dess, Dennis Vanderweele, Liquan Huang, Hong Wang, Danielle Reed

Clinton D Chapman

Nearly all mammalian species like sweet-tasting foods and drinks, but there are differences in the degree of 'sweet tooth' both between species and among individuals of the same species. Some individual differences can be explained by genetic variability. Polymorphisms in a sweet taste receptor (Tas1r3) account for a large fraction of the differences in consumption of sweet solutions among inbred mouse strains. We wondered whether mice and rats share the same Tas1r3 alleles, and whether this gene might explain the large difference in saccharin preference among rats. We conducted three experiments to test this. We examined DNA sequence differences in …

What Communities Should Do Pre-Event To Support Public Health Post-Event Assessments, Surveillance And Monitoring, Thomas Lyons Carr Iii

Thomas Lyons (Thom) Carr III Appl.Sc., CEM

[Abstract written March 2008, TLC] Under worst-case planning assumptions used by some major metropolitan areas, a Neighbor-to-Neighbor self-help program model is the primary link between citizens and the professional response personnel of the responsible government agencies.

In the Neighbor-to-Neighbor self-help program model or a Community Emergency Management Plan (CEMP) calls on the citizens in neighborhoods to identify and establish cluster emergency preparedness committees, Cluster Emergency Coordination Centers (CECC) and Community Emergency Response Teams (CERT). Missing from these plans or what is not articulated is how constant Public Health Post-Event Surveillance, Monitoring and Assessments will be done. Given the worst-case planning …

Electromyographical Analysis And Comparison Of Selected Abdominal Training Devices With A Traditional Crunch, Stuart Rugg

Stuart Rugg

The present study compared the muscle activity elicited by selected abdominal training devices with muscle activity elicited by a traditional crunch. Forty-six adults participated in the study. The exercise devices tested included the Ab-ONE, Perfect Abs Roller, Ab Scissor, Ab Swing, 6SecondAbs, and the Torso Track. For those devices with variable resistances, all were tested at their high resistance setting. Surface electromyography (EMG) was recorded from the upper and lower portions of the rectus abdominis and the external oblique during each repetition. EMG values were analyzed using repeated measures analyses of variance (ANOVA) and pairwise comparisons. EMG measurements for each …

Biogeography Of The Nearshore Rocky-Reef Fishes At The Southern And Baja California Islands, Daniel Pondella, Brooke Gintert, Jana Cobb, Larry Allen

Daniel Pondella

No abstract provided.

The Proto-Oncogene C-Kit Maps To Canid B-Chromosomes, Alexander S. Graphodatsky, Anna V. Kukekova, Dmitry V. Yudkin, Vladimir A. Trifonov, Nadezhda V. Vorobieva, Violetta R. Beklemisheva, Polina L. Perelman, Daria A. Graphodatskaya, Lyudmila N. Trut, Fengtang Yang, Malcolm A. Ferguson-Smith, Gregory M. Acland, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Quantitative Nonisotopic Nitrocellulose Filter Binding Assays: Bacterial Manganese Superoxide Dismutase–Dna Interactions, Joshua Czerwinski, Stephanie Hovan, David Mascotti

David P. Mascotti

Nitrocellulose filter binding assays (NCFBAs) have been used for many years to qualitatively and quantitatively determine protein–nucleic acid affinities. While this technique can be robust thermodynamically and fairly simple to perform, the requirement of radiolabeled nucleic acids (typically 32P) has several major drawbacks. Some disadvantages are the short half-life of 32P, the inherent safety concerns, and the cost of working with radioisotopes. Another drawback is that over time the beta emissions cause fragmentation of the nucleic acids. We have modified standard NCFBAs by developing a quantitative nonisotopic chemiluminescent method using biotin-labeled DNA and a dual-filter format. The biotin tag is …

Plethodon Albagula (Western Slimy Salamander), Vocalization, Joseph Milanovich

Joseph Milanovich

No abstract provided.

Presence And Colonization Of Placobdella On Two Species Of Freshwater Turtles (Graptemys Geographica And Sternotherus Odoratus), Travis Ryan

Travis J. Ryan

It is generally accepted that bottom-dwelling turtles have a higher ectoparasite load than turtles that bask aerially because of effects of desiccation on ectoparasites, especially with regard to leeches. We compared number of leeches (primarily Placobdella parasitica) on field-caught Common Musk Turtles (Stemotherus odoratus) and Common Map Turtles (Graptemys geographica). The bottom-dwelling species S. odoratus had more than 20 times the number of leeches than the aerial-basking species G. geographica. We then exposed cleaned (leech-free) turtles to leeches in mesocosms (cattle tanks) to measure the rate of colonization. In this experiment, S. odoratus had more than four times the number …

Sport In Canada: A History, Donald Morrow, Kevin Wamsley

Donald Morrow

No abstract provided.

Bioinformatic Analysis Of The Metal-Binding Protein Families And Heavy Metal Resistance Amongst Cyanobacteria, Tin-Chun Chu, Lee Lee, Shankar Srinivasan

Tin-Chun Chu, Ph.D.

No abstract provided.

Castration Inhibits The Exercise-Induced Accumulation Of Hsp70 In Male Rodent Cardiac Muscle Tissue, Kevin Milne, David Thorp, C.W. Melling, Earl Noble

Jamie Melling

Intense exercise leads to accumulation of the inducible member of the 70-kDa family of heat shock proteins, Hsp70, in male, but not female, hearts. Estrogen is at least partially responsible for this difference. Because androgen receptors are expressed in the heart and castration leads to decreases in calcium regulatory proteins and altered cardiac function, testosterone (T) or its metabolites could also be involved. We hypothesized that removal of endogenous T production through castration would reduce cardiac Hsp70 accumulation after an acute exercise bout, whereas castrated animals supplemented with 5α-dihydrotestosterone (DHT) would show the intact male response. Fifty-four 8-wk-old male Sprague-Dawley …

Diadophis Punctatus Sticktogenys (Mississippi Ringneck Snake), Diet, Joseph Milanovich

Joseph Milanovich

No abstract provided.

Talking Politics And Engaging Politics: An Examination Of The Interactive Relationships Between Structural Features Of Political Talk And Discussion Engagement, Ann Williams

Ann E Williams

This study takes a process-oriented approach to understand the current status of political discussion research and identifies discussion engagement—discussion attention and integrative discussion—as an unexplored but important facet of political discussion. As a block, these two variables of discussion engagement independently accounted for significant variance in two criterion variables of political engagement, political knowledge and political participation, after controlling for not only a host of demographic, attitudinal, and media use variables but also three most researched structural features of political discussion—network size, discussion frequency, and network heterogeneity. In addition, the study analyzes the interplay between various attributes of political discussion …

Interaction Between The Dorsal And Ventral Pathways In Mental Rotation: An Fmri Study, Hideya Koshino, Patricia Carpenter, Timothy Keller, Marcel Just

Marcel Adam Just

No abstract provided.