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Purdue University

2016

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Articles 211 - 237 of 237

Full-Text Articles in Life Sciences

Midwest Cantaloupe Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie Jan 2016

Midwest Cantaloupe Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie

Purdue Fruit and Vegetable Research Reports

This is a compilation of 21 research trial reports from four land-grant universities in the Midwestern United States. Crops include cantaloupe, pickling cucumber, pepper, potato, pumpkin, summer squash and zucchini, sweet corn, tomato, and watermelon. Somecrops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties. One report addressed plant spacing for sweet corn and one addressed soil block for production of tomato seedlings. A list of vegetable seed sources and a list of other online sources of vegetable trial reports are also included.


Structural Basis For Recognition Of Human Enterovirus 71 By A Bivalent Broadly Neutralizing Monoclonal Antibody, Xiaohua Ye, Chen Fan, Zhiqiang Ku, Teng Zuo, Liangliang Kong, Chao Zhang, Jinping Shi, Qingwei Liu, Tan Chen, Yingyi Zhang, Wen Jiang, Linqi Zhang, Zhong Huang, Yao Cong Jan 2016

Structural Basis For Recognition Of Human Enterovirus 71 By A Bivalent Broadly Neutralizing Monoclonal Antibody, Xiaohua Ye, Chen Fan, Zhiqiang Ku, Teng Zuo, Liangliang Kong, Chao Zhang, Jinping Shi, Qingwei Liu, Tan Chen, Yingyi Zhang, Wen Jiang, Linqi Zhang, Zhong Huang, Yao Cong

Department of Biological Sciences Faculty Publications

Enterovirus 71 (EV71) is the main pathogen responsible for hand, foot and mouth disease with severe neurological complications and even death in young children. We have recently identified a highly potent anti-EV71 neutralizing monoclonal antibody, termed D5. Here we investigated the structural basis for recognition of EV71 by the antibody D5. Four three-dimensional structures of EV71 particles in complex with IgG or Fab of D5 were reconstructed by cryo-electron microscopy (cryo-EM) single particle analysis all at subnanometer resolutions. The most critical EV71 mature virion-Fab structure was resolved to a resolution of 4.8 Å, which is rare in cryo-EM studies of …


Missing Gene Identification Using Functional Coherence Scores, Meghana Chitale, Ishita K. Khan, Daisuke Kihara Jan 2016

Missing Gene Identification Using Functional Coherence Scores, Meghana Chitale, Ishita K. Khan, Daisuke Kihara

Department of Biological Sciences Faculty Publications

Reconstructing metabolic and signaling pathways is an effective way of interpreting a genome sequence. A challenge in a pathway reconstruction is that often genes in a pathway cannot be easily found, reflecting current imperfect information of the target organism. In this work, we developed a new method for finding missing genes, which integrates multiple features, including gene expression, phylogenetic profile, and function association scores. Particularly, for considering function association between candidate genes and neighboring proteins to the target missing gene in the network, we used Co-occurrence Association Score (CAS) and PubMed Association Score (PAS), which are designed for capturing functional …


The Effect Of Adjuvants On Apple Disease Management, Chelsi Patricia Abbott Jan 2016

The Effect Of Adjuvants On Apple Disease Management, Chelsi Patricia Abbott

Open Access Theses

The management of common apple diseases such as apple scab ( Venturia inaequalis) and bitter rot (Colletotrichum spp.) relies heavily on effective fungicide applications. However, the development of fungicide resistance to newer fungicides has resulted in management failures and significant economic losses. This has led to a greater reliance on captan, an older fungicide, because there is a low risk of pathogens developing resistance. Label restrictions limit growers to 18 kg of captan per season, which may not provide sufficient control of both apple scab and bitter rot in wet years. Consequently, apple growers are faced with two …


Development Of The Neuron Assessment For Measuring Biology Students’ Use Of Experimental Design Concepts And Representations, Annwesa P. Dasgupta, Trevor R. Anderson, Nancy J. Pelaez Jan 2016

Development Of The Neuron Assessment For Measuring Biology Students’ Use Of Experimental Design Concepts And Representations, Annwesa P. Dasgupta, Trevor R. Anderson, Nancy J. Pelaez

PIBERG Publications

Researchers, instructors, and funding bodies in biology education are unanimous about the importance of developing students’ competence in experimental design. Despite this, only limited measures are available for assessing such competence development, especially in the areas of molecular and cellular biology. Also, existing assessments do not measure how well students use standard symbolism to visualize biological experiments. We propose an assessment-design process that 1) provides background knowledge and questions for developers of new “experimentation assessments,” 2) elicits practices of representing experiments with conventional symbol systems, 3) determines how well the assessment reveals expert knowledge, and 4) determines how well the …


Differential Gene Expression In Varroa Jacobsoni Mites Following A Host Shift To European Honey Bees (Apis Mellifera), Gladys K. Andino, Michael R. Gribskov, Denis L. Anderson, Jay D. Evans, Greg Hunt Jan 2016

Differential Gene Expression In Varroa Jacobsoni Mites Following A Host Shift To European Honey Bees (Apis Mellifera), Gladys K. Andino, Michael R. Gribskov, Denis L. Anderson, Jay D. Evans, Greg Hunt

Department of Entomology Faculty Publications

Background: Varroa mites are widely considered the biggest honey bee health problem worldwide. Until recently, Varroa jacobsoni has been found to live and reproduce only in Asian honey bee (Apis cerana) colonies, while V. destructor successfully reproduces in both A. cerana and A. mellifera colonies. However, we have identified an island population of V. jacobsoni that is highly destructive to A. mellifera, the primary species used for pollination and honey production. The ability of these populations of mites to cross the host species boundary potentially represents an enormous threat to apiculture, and is presumably due to genetic variation that exists …


Metatranscriptome Analysis Reveals Bacterial Symbiont Contributions To Lower Termite Physiology And Potential Immune Functions., Brittany F. Peterson, Michael E. Scharf Jan 2016

Metatranscriptome Analysis Reveals Bacterial Symbiont Contributions To Lower Termite Physiology And Potential Immune Functions., Brittany F. Peterson, Michael E. Scharf

Department of Entomology Faculty Publications

Background: Symbioses throughout the animal kingdom are known to extend physiological and ecological capabilities to hosts. Insect-microbe associations are extremely common and are often related to novel niche exploitation, fitness advantages, and even speciation events. These phenomena include expansions in host diet, detoxification of insecticides and toxins, and increased defense against pathogens. However, dissecting the contributions of individual groups of symbionts at the molecular level is often underexplored due to methodological and analytical limitations. Termites are one of the best studied systems for physiological collaborations between host and symbiota however, most work in lower termites (those with bacterial and protist …


Changes In The Proteome Of Langat-Infected Ixodes Scapularis Ise6 Cells: Metabolic Pathways Associated With Flavivirus Infection, Jeffrey M. Grabowski, Rushika Perera, Ali M. Roumani, Victoria E. Hedrick, Halina Dorota Inerowicz, Catherine A. Hill, Richard Kuhn Jan 2016

Changes In The Proteome Of Langat-Infected Ixodes Scapularis Ise6 Cells: Metabolic Pathways Associated With Flavivirus Infection, Jeffrey M. Grabowski, Rushika Perera, Ali M. Roumani, Victoria E. Hedrick, Halina Dorota Inerowicz, Catherine A. Hill, Richard Kuhn

Department of Entomology Faculty Publications

Background: Ticks (Family Ixodidae) transmit a variety of disease causing agents to humans and animals. The tick-borne flaviviruses (TBFs; family Flaviviridae) are a complex of viruses, many of which cause encephalitis and hemorrhagic fever, and represent global threats to human health and biosecurity. Pathogenesis has been well studied in human and animal disease models. Equivalent analyses of tick-flavivirus interactions are limited and represent an area of study that could reveal novel approaches for TBF control. Methodology/Principal Findings: High resolution LC-MS/MS was used to analyze the proteome of Ixodes scapularis (Lyme disease tick) embryonic ISE6 cells following infection with Langat virus …


Midwest Vegetable Trial Report For 2015, Elizabeth Maynard Jan 2016

Midwest Vegetable Trial Report For 2015, Elizabeth Maynard

Midwest Vegetable Trial Reports

This is a compilation of 19 research trial reports from six land-grant universities in the midwestern United States. Crops include bok choy, cantaloupe, cucumber, pepper, pumpkin, sweet corn, squash, tomato, and watermelon. Several crops were evaluated in high tunnels or hoophouses. Most trials evaluated different cultivars or varieties, including one investigating cantaloupe variety influence on cucumber beetle presence and incidence of bacterial wilt. Four reports addressed specific growing practices: the use of poly-coated urea to supply nitrogen to sweet corn, biochar use in a high tunnel bag culture system, use of cereal rye cover crop in butternut squash, and pollinizer …


Michigan State University 2015 Seedless Pickling Cucumber Variety Trial, Ben Phillips Jan 2016

Michigan State University 2015 Seedless Pickling Cucumber Variety Trial, Ben Phillips

Midwest Vegetable Trial Reports

No abstract provided.


Hot Pepper Cultivar Evaluation Using Extension Master Gardeners, G.A. Pabodha Galgamuwa, Charles J. Barden, Ward Upham Jan 2016

Hot Pepper Cultivar Evaluation Using Extension Master Gardeners, G.A. Pabodha Galgamuwa, Charles J. Barden, Ward Upham

Midwest Vegetable Trial Reports

No abstract provided.


Poly-Coated Urea As A Seasonlong Nitrogen Source For Sweet Corn, Ron Goldy, Carly Andres, Virginia Wendzel Jan 2016

Poly-Coated Urea As A Seasonlong Nitrogen Source For Sweet Corn, Ron Goldy, Carly Andres, Virginia Wendzel

Midwest Vegetable Trial Reports

No abstract provided.


Performance Of 19 Fresh Market And Six Saladette Tomato Cultivars In Southwest Michigan In 2015, Ron Goldy, Virginia Wendzel Jan 2016

Performance Of 19 Fresh Market And Six Saladette Tomato Cultivars In Southwest Michigan In 2015, Ron Goldy, Virginia Wendzel

Midwest Vegetable Trial Reports

No abstract provided.


Evaluation Of Softneck And Hardneck Garlic Produced Within Or Without High Tunnels, Lewis Jett, Larry Campbell, John Murray Jan 2016

Evaluation Of Softneck And Hardneck Garlic Produced Within Or Without High Tunnels, Lewis Jett, Larry Campbell, John Murray

Midwest Vegetable Trial Reports

No abstract provided.


Improved Prediction Of Severe Thunderstorms Over The Indian Monsoon Region Using High-Resolution Soil Moisture And Temperature Initialization, K. K. Osuri, R. Nadimpalli, U. C. Mohanty, F. Chen, M. Rajeevan, Dev Niyogi Jan 2016

Improved Prediction Of Severe Thunderstorms Over The Indian Monsoon Region Using High-Resolution Soil Moisture And Temperature Initialization, K. K. Osuri, R. Nadimpalli, U. C. Mohanty, F. Chen, M. Rajeevan, Dev Niyogi

Department of Earth, Atmospheric, and Planetary Sciences Faculty Publications

The hypothesis that realistic land conditions such as soil moisture/soil temperature (SM/ST) can significantly improve the modeling of mesoscale deep convection is tested over the Indian monsoon region (IMR). A high resolution (3 km foot print) SM/ST dataset prepared from a land data assimilation system, as part of a national monsoon mission project, showed close agreement with observations. Experiments are conducted with (LDAS) and without (CNTL) initialization of SM/ST dataset. Results highlight the significance of realistic land surface conditions on numerical prediction of initiation, movement and timing of severe thunderstorms as compared to that currently being initialized by climatological fields …


A Study Of Alterations In Dna Epigenetic Modifications (5mc And 5hmc) And Gene Expression Influenced By Simulated Microgravity In Human Lymphoblastoid Cells, Basudev Chowdhury, Arun Seetharam, Zhiping Wang, Yunlong Liu, Amy C. Lossie, Jyothi Thimmapuram Jan 2016

A Study Of Alterations In Dna Epigenetic Modifications (5mc And 5hmc) And Gene Expression Influenced By Simulated Microgravity In Human Lymphoblastoid Cells, Basudev Chowdhury, Arun Seetharam, Zhiping Wang, Yunlong Liu, Amy C. Lossie, Jyothi Thimmapuram

Cyber Center Publications

Cells alter their gene expression in response to exposure to various environmental changes. Epigenetic mechanisms such as DNA methylation are believed to regulate the alterations in gene expression patterns. In vitro and in vivo studies have documented changes in cellular proliferation, cytoskeletal remodeling, signal transduction, bone mineralization and immune deficiency under the influence of microgravity conditions experienced in space. However microgravity induced changes in the epigenome have not been well characterized. In this study we have used Next-generation Sequencing (NGS) to profile ground-based “simulated” microgravity induced changes on DNA methylation (5-methylcytosine or 5mC), hydroxymethylation (5-hydroxymethylcytosine or 5hmC), and simultaneous gene …


Regulation Of Brown And White Adipocyte Transcriptome By The Transcriptional Coactivator Nt-Pgc-1Α, Jihyun Kim, Vivian E. Fernand, Tara M. Henagan, Jeho Shin, Peter Huypens, Susan Newman, Thomas W. Gettys, Ji Suk Chang Jan 2016

Regulation Of Brown And White Adipocyte Transcriptome By The Transcriptional Coactivator Nt-Pgc-1Α, Jihyun Kim, Vivian E. Fernand, Tara M. Henagan, Jeho Shin, Peter Huypens, Susan Newman, Thomas W. Gettys, Ji Suk Chang

Department of Nutrition Science Faculty Publications

The β3-adrenergic receptor (AR) signaling pathway is a major component of adaptive thermogenesis in brown and white adipose tissue during cold acclimation. The β3-AR signaling highly induces the expression of transcriptional coactivator PGC-1α and its splice variant N-terminal (NT)-PGC-1α, which in turn activate the transcription program of adaptive thermogenesis by co-activating a number of transcription factors. We previously reported that NT-PGC-1α is able to increase mitochondrial number and activity in cultured brown adipocytes by promoting the expression of mitochondrial and thermogenic genes. In the present study, we performed genome-wide profiling of NT-PGC-1α-responsive genes in brown adipocytes to identify genes potentially …


Unique Behavioral And Neurochemical Effects Induced By Repeated Adolescent Consumption Of Caffeine-Mixed Alcohol In C57bl/6 Mice., Meridith T. Robins, Julie Lu, Richard M. Van Rijn Jan 2016

Unique Behavioral And Neurochemical Effects Induced By Repeated Adolescent Consumption Of Caffeine-Mixed Alcohol In C57bl/6 Mice., Meridith T. Robins, Julie Lu, Richard M. Van Rijn

Department of Medicinal Chemistry and Molecular Pharmacology Faculty Publications

The number of highly caffeinated products has increased dramatically in the past few years. Among these products, highly caffeinated energy drinks are the most heavily advertised and purchased, which has resulted in increased incidences of co-consumption of energy drinks with alcohol. Despite the growing number of adolescents and young adults reporting caffeine-mixed alcohol use, knowledge of the potential consequences associated with co-consumption has been limited to survey-based results and in-laboratory human behavioral testing. Here, we investigate the effect of repeated adolescent (post-natal days P35-61) exposure to caffeine-mixed alcohol in C57BL/6 mice on common drug-related behaviors such as locomotor sensitivity, drug …


Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas Jan 2016

Sugar-Enhanced And Synergistic Sweet Corn Cultivar Evaluation For Northern Indiana, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

This paper reports on seven bicolor and two yellow sugar-enhanced or synergistic sweet corn entries that were evaluated at the Pinney-Purdue Agricultural Center in Wanatah, Indiana.


Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas Jan 2016

Mini- And Heirloom Sweet Pepper Variety Performance In High Tunnels, 2015, Elizabeth Maynard, Israel S. Calsoyas

Midwest Vegetable Trial Reports

Trials on mini sweet peppers and other specialty sweet peppers in the Midwest have not recently been reported. We undertook this project to compare yield and fruit characteristics of hybrid and heirloom peppers grown in high tunnels using organic and conventional production methods.


Midwest Cantaloupe Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie Jan 2016

Midwest Cantaloupe Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie

Midwest Vegetable Trial Reports

No abstract provided.


Cantaloupe Variety Trial For Kentucky, 2015, Shubin K. Saha, John Snyder, Chris Smigell, John Walsh Jan 2016

Cantaloupe Variety Trial For Kentucky, 2015, Shubin K. Saha, John Snyder, Chris Smigell, John Walsh

Midwest Vegetable Trial Reports

No abstract provided.


Comparison Of Attractiveness And Reaction Of Melon Cultivars To The Striped Cucumber Beetle And Bacterial Wilt, 2015, Ahmad Shah Mohammadi, Ricky E. Foster, Elizabeth Maynard, Dan Egel Jan 2016

Comparison Of Attractiveness And Reaction Of Melon Cultivars To The Striped Cucumber Beetle And Bacterial Wilt, 2015, Ahmad Shah Mohammadi, Ricky E. Foster, Elizabeth Maynard, Dan Egel

Midwest Vegetable Trial Reports

No abstract provided.


2015 Butternut Squash Cereal Rye Cover Crop Trial, Ben Phillips Jan 2016

2015 Butternut Squash Cereal Rye Cover Crop Trial, Ben Phillips

Midwest Vegetable Trial Reports

No abstract provided.


2015 Bt Sh2 Sweet Corn Variety Trial, Ben Phillips Jan 2016

2015 Bt Sh2 Sweet Corn Variety Trial, Ben Phillips

Midwest Vegetable Trial Reports

No abstract provided.


Midwest Triploid Watermelon Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie Jan 2016

Midwest Triploid Watermelon Variety Trial In Southwest Indiana — 2015, Wenjing Guan, Dan Egel, Dennis Nowaskie

Midwest Vegetable Trial Reports

No abstract provided.


Evaluation Of Three Pollinizer Planting Patterns On Seedless Watermelon Yield, Ron Goldy Jan 2016

Evaluation Of Three Pollinizer Planting Patterns On Seedless Watermelon Yield, Ron Goldy

Midwest Vegetable Trial Reports

No abstract provided.