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- DNA fragmentation (2)
- Motility (2)
- 8-oxodGuo (1)
- Assay (1)
- Biomarker (1)
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- Bulky DNA adducts (1)
- Chromatin structure (1)
- Coke oven workers (1)
- DNA integrity (1)
- Human fertility (1)
- Hydrocarbons (1)
- Metals (1)
- Morphology (1)
- Oxidative stress (1)
- Polycyclic aromatic (1)
- Polycyclic aromatic hydrocarbons (1)
- Semen quality (1)
- Solid phase extraction (1)
- Sperm concentration (1)
- Stress (1)
- Urinary (1)
- Viability (1)
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Role Of Low Exposure To Metals As Male Reproductive Toxicants, H. Anna Jeng, Yeou-Lih Huang, Chih-Hong Pan, Norou Diawara
Role Of Low Exposure To Metals As Male Reproductive Toxicants, H. Anna Jeng, Yeou-Lih Huang, Chih-Hong Pan, Norou Diawara
Community & Environmental Health Faculty Publications
The objective of the study was to examine the associations between environmentally relevant low metal concentrations and semen quality parameters in men. The concentrations of zinc (Zn), copper (Cu), cadmium (Cd), arsenic (As), selenium (Se), and lead (Pb) in the seminal plasma and urine were measured from 196 male human subjects in Taiwan. Urinary Cd concentrations were negatively associated with sperm viability (p=0.006). Seminal plasma Cu concentrations of the normal group (>= 15 x 10(6)/ml) were significantly lower than those of the abnormal group (p=0.023). However, the linear regression analysis showed a weak association between Cu concentration and sperm …
Sperm Dna Oxidative Damage And Dna Adducts, Hueiwang Anna Jeng, Chih-Hong Pan, Mu-Rong Chao, Wen-Yi Lin
Sperm Dna Oxidative Damage And Dna Adducts, Hueiwang Anna Jeng, Chih-Hong Pan, Mu-Rong Chao, Wen-Yi Lin
Community & Environmental Health Faculty Publications
The objective of this study was to investigate DNA damage and adducts in sperm from coke oven workers who have been exposed to polycyclic aromatic hydrocarbons. A longitudinal study was conducted with repeated measurements during spermatogenesis. Coke-oven workers (n =112) from a coke-oven plant served the PAH-exposed group, while administrators and security personnel (n=67) served the control. Routine semen parameters (concentration, motility, vitality, and morphology) were analyzed simultaneously; the assessment of sperm DNA integrity endpoints included DNA fragmentation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGuo). The degree of sperm DNA fragmentation was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling …
Are Semen Quality Parameters Sufficient For Biomonitoring Spermatozoa Dna Integrity And Oxidatively Damaged Dna, Hueiwang Anna Jeng, Ruei-Nian Li, Wen-Yi Lin
Are Semen Quality Parameters Sufficient For Biomonitoring Spermatozoa Dna Integrity And Oxidatively Damaged Dna, Hueiwang Anna Jeng, Ruei-Nian Li, Wen-Yi Lin
Community & Environmental Health Faculty Publications
The present study aimed to investigate the relationship between semen quality parameters and DNA integrity, and determine whether semen quality parameters could serve as a reliable biomarker for monitoring sperm DNA damage. Conventional semen parameters from a total of 202 male human subjects were analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′- deoxyguanosine (8-oxoGuo) were used to assess sperm DNA integrity. DNA fragmentation was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and sperm chromatin structure assay (SCSA), while 8-oxodGuo was quantified by the liquid chromatography/tandem mass spectrometry (LC-MS/MS) coupled with an on-line solid phase system. The levels of …