Life Sciences Commons^™

Donor Platelet Plasma Components Inactivate Sensitive And Multidrug Resistant Acinetobacter Baumannii Isolates, Chelsea M. Edelblute, Olga N. Pakhomova, Fanying Li, Barbara Y. Hargrave, Loree C. Heller

Bioelectrics Publications

Acinetobacter baumannii is an environmentally resilient healthcare-associated opportunistic pathogen responsible for infections at many body sites. In the last 10 years, clinical strains resistant to many or all commonly used antibiotics have emerged globally. With few antimicrobial agents in the pharmaceutical pipeline, new and alternative agents are essential. Platelets secrete a large number of proteins, including proteins with antimicrobial activity. In a previous study, we demonstrated that donor platelet supernatants and plasma significantly inhibited the growth of a reference strain of A. baumannii in broth and on skin. This inhibition appeared to be unrelated to the platelet activation state. In …

P53: "The Wall Watcher", Nektarios Barabutis, John D. Catravas

Bioelectrics Publications

No abstract provided.

Virginia’S Amphibians: Status, Threats And Conservation, Jennifer Sevin, John D. Kleopfer

Virginia Journal of Science

Virginia’s diverse environments support 84 amphibian species (anurans and caudates), making it the third highest state in terms of species richness. However, the Commonwealth matches the global trend in declining amphibian populations with over one-third of its amphibian species in conservation need. The Species of Greatest Conservation Need included in the most recent Virginia Wildlife Action Plan cut across amphibian families and ecoregions. It is challenging to ascertain the exact cause of most of the population declines. In one degree or another, all of the global threats to amphibians exist within Virginia’s borders. While an active research program on amphibians …

Forest Diversity And Disturbance: Changing Influences And The Future Of Virginia’S Forests, Christine J. Small, James L. Chamberlain

Virginia Journal of Science

The Virginia landscape supports a remarkable diversity of forests, from maritime dune woodlands, swamp forests, and pine savannas of the Atlantic Coastal Plain, to post-agricultural pine-hardwood forests of the Piedmont, to mixed oak, mesophytic, northern hardwood, and high elevation spruce-fir forests across three mountain provinces in western parts of the state. Virginia’s forests also have been profoundly shaped by disturbance. Chestnut blight, hemlock woolly adelgid, emerald ash borer, and other pests have caused declines or functional extirpation of foundation species. Invasive plants like multiflora rose, Oriental bittersweet, and Japanese stiltgrass threaten both disturbed and intact forests. Oaks and other fire-dependent …

Freshwater Mussels Of Virginia (Bivalvia: Unionidae): An Introduction To Their Life History, Status And Conservation, Jess W. Jones

Virginia Journal of Science

With 77 species, the mussel fauna of Virginia is one of the most diverse in the United States. Fifty-four species or ~70% of the state’s mussel fauna occurs in the rivers of the upper Tennessee River basin, especially in the Clinch and Powell rivers of southwestern Virginia. An additional 23 species reside in rivers of the Atlantic Slope, including the Potomac, Rappahannock, York, James and Chowan basins, and in the New River, a major tributary to the Ohio River. A total of 39 species or 51% of Virginia’s mussel fauna is listed as federally endangered, state endangered or state threatened. …

Breeding Birds Of Virginia, Bryan D. Watts

Virginia Journal of Science

Virginia supports a diverse community of breeding birds that has been the focus of investigation for more than 400 years. The avifauna reflects the latitudinal position of the state and the fact that the border extends from the Atlantic Ocean to the Appalachian Mountains. A total of 224 species have been recorded breeding in Virginia, 214 of which are extant. Twenty species have colonized the state since 1900 including 14 since 1950. Of all extant species, 102 (48%) are considered common at least somewhere in the state and 64 (30%) are rare to very rare. Diversity varies by physiographic region …

Status Of Plants In Virginia, Michael H. Renfroe

Virginia Journal of Science

OVERVIEW OF BOTANICAL DIVERSITY

Virginia possesses a unique and varied assemblage of plant life. There are 3,164 species, subspecies and varieties of plants in Virginia (Weakley et al. 2012). As classified by the Virginia Department of Conservation and Recreation’s Division of Natural Heritage (DCR-DNH), they form some 94 ecological groups and 317 community types across five distinct physiographic provinces: Coastal Plain, Piedmont, Blue Ridge, Ridge and Valley, and Appalachian Plateau. The state extends 469 miles from east to west and 201 miles north to south at the widest points, enclosing 42,326 square miles of territory. This diverse range of environmental …

Virginia’S Land Mammals: Past And Present, With Some Thoughts About Their Possible Future, John F. Pagels, Nancy D. Moncrief

Virginia Journal of Science

Mammals encountered today in Virginia’s forests and fields include native and nonnative species, feral populations, and free-ranging pets. We examine factors that have influenced Virginia’s terrestrial mammal fauna since the arrival of European colonists in the 1600s and some of the factors that are shaping the fauna today. We look in depth at changes since Handley and Patton’s (1947) first complete monograph on Virginia mammals and augment Linzey’s (1998) book, The Mammals of Virginia. We include current nomenclature, baseline information, and references to comprehensive literature. We discuss some of the current and developing anthropogenic factors that have impacted, or that …

Temporal Variability And Cooperative Breeding: Testing The Bet-Hedging Hypothesis In The Acorn Woodpecker, Walter D. Koenig, Eric L. Walters

Biological Sciences Faculty Publications

Cooperative breeding is generally considered an adaptation to ecological constraints on dispersal and independent breeding, usually due to limited breeding opportunities. Although benefits of cooperative breeding are typically thought of in terms of increased mean reproductive success, it has recently been proposed that this phenomenonmay be a bet-hedging strategy that reduces variance in reproductive success (fecundity variance) inpopulations living inhighly variable environments. We tested this hypothesis using long-term data on the polygynandrous acorn woodpecker (Melanerpes formicivorus). In general, fecundity variance decreased with increasing sociality, at least when controlling for annual variation in ecological conditions. Nonetheless, decreased fecundity variance …

Viewing The Status Of Virginia’S Environment Through The Lens Of Freshwater Fishes, Paul L. Angermeier, Michael J. Pinder

Virginia Journal of Science

We summarize a range of topics related to the status of Virginia’s freshwater fishes, their reflection of environmental quality, and their contribution to human wellbeing. Since 1994 the list of extant Virginia fishes has lengthened from 210 species to 227 species, mostly due to taxonomic reorganizations. Virginia’s list of Species of Greatest Conservation Need currently contains 96 fish species, predominated by darters (32 species) and minnows (28 species). Increasing trends in species rarity and threats to fishes suggest that Virginia’s aquatic environment is becoming less hospitable for fishes. Prevailing anthropogenic threats to fishes include agriculture, urban development, mineral extraction, forestry, …

The Effects Of Urbanization On Tick Parasitism Rates In Birds Of Southeastern Virginia, Erin Leigh Heller

Biological Sciences Theses & Dissertations

The coastal region of southeastern Virginia is one of the largest urban areas along one of North America’s migratory flyways. Because hundreds of avian species use this flyway, understanding factors affecting birds and their health is of paramount concern. Within this region, 14 species of ticks have been documented, all of which may serve as vectors of mammal (including human) pathogens. By sampling birds at sites of varying levels of urbanization within the coastal southeastern urban matrix, I studied the relationship between ticks and their avian hosts, and how this relationship varies seasonally. Mistnets were set-up at five permanent sites …

Kinematics And Hydrodynamics Of Cephalopod Turning Performance In Routine Swimming And Predatory Attacks, Rachel A. Jastrebsky

Biological Sciences Theses & Dissertations

Steady rectilinear swimming has received considerable attention in aquatic animal locomotion studies. Unsteady swimming movements, however, represent a large portion of many aquatic animals’ locomotive repertoire and have not been examined extensively. This study incorporates kinematic analyses of routine turning performance of brief squid Lolliguncula brevis and dwarf cuttlefish Sepia bandensis (Chapter 2), 3D velocimetry techniques to examine hydrodynamic turning performance of L. brevis (Chapter 3) and kinematic analyses of turning performance of L. brevis during predatory attacks on shrimp and fish prey (Chapter 4).

Both L. brevis and S. bandensis demonstrated high maneuverability, having the lowest measures of length-specific …

Groundwater Thresholds For Root Decomposition And The Relation To Barrier Island Ecological State Changes, Matthew Lee Smith

Biological Sciences Theses & Dissertations

Barrier islands off the eastern shore of Virginia exhibit distinct habitats that abruptly transition between periodically brackish/freshwater marshes, wooded swales, and sparsely vegetated dunes. There is strong evidence that the plant communities and ecosystem processes occurring in each habitat are primarily influenced by nutrient availability and the distance between two of the three free surfaces: land and freshwater. At the Virginia Coast Reserve-Long Term Ecological Research Site in Virginia, USA, thresholds to belowground decomposition rates were identified by measuring decay of native roots and rhizomes at 32 elevations in relation to mean annual groundwater levels (-0.356 – 1.937 m). Negative …

Dna Repair Deficiency In Huntington's Disease Fibroblasts And Induced Pluripotent Stem Cells, Peter Anthony Mollica

Biological Sciences Theses & Dissertations

Mutant huntingtin protein (mhtt)– the protein responsible for cellular dysfunction in Huntington’s disease (HD) –is a product of an expanded trinucleotide repeat (TNR) cytosine-adenine-guanine (CAG) sequence in exon 1 of the huntingtin (HTT) gene. The pathology of HD has been extensively researched; however, the mechanism by which the disease-causing TNR expansions occur in somatic cells remains elusive. Interestingly, HD has often been referred to a ‘DNA repair disease’, even though DNA repair dysfunction in situ has not been identified. We hypothesized that presence of the mhtt protein affects the expression of DNA repair genes used to address DNA repair, ultimately …

Efficient Algorithms For Prokaryotic Whole Genome Assembly And Finishing, Abhishek Biswas

Computer Science Theses & Dissertations

De-novo genome assembly from DNA fragments is primarily based on sequence overlap information. In addition, mate-pair reads or paired-end reads provide linking information for joining gaps and bridging repeat regions. Genome assemblers in general assemble long contiguous sequences (contigs) using both overlapping reads and linked reads until the assembly runs into an ambiguous repeat region. These contigs are further bridged into scaffolds using linked read information. However, errors can be made in both phases of assembly due to high error threshold of overlap acceptance and linking based on too few mate reads. Identical as well as similar repeat regions can …

Wildlife Crime And Other Challenges To Resource System Resilience, Patricia Anne Raxter

Graduate Program in International Studies Theses & Dissertations

Although wildlife crime has exploded in Africa over the past decade —“commercial poaching” now kills an estimated eight percent of the continent’s elephant population each year—some governments have proven more successful than others at protecting wildlife and preserving habitats. To explain this variation, this study examines how the policies of three states (Kenya, Tanzania, and Botswana) have enhanced or undermined the resilience of the continent’s elephant ecosystem. Using the social-ecological system framework, the study illustrates how each state’s changing practices have either exacerbated the stresses wrought by wildlife crime or successfully protected local populations from poaching. The study finds that …

Mate Choice In Temperate And Tropical Spiny Lobsters With Contrasting Reproductive Systems, Mark Butler Iv, Rodney Bertelsen, Alison Macdiarmid

Biological Sciences Faculty Publications

Sperm limitation of reproductive success is common in decapod crustaceans, favouring mating systems in which females compete for large males of high reproductive value. We investigated these phenomena in two species of spiny lobsters—one temperate, one tropical—with contrasting reproductive systems: the Southern Rock Lobster (Jasus edwardsii) and the Caribbean Spiny Lobster (Panulirus argus). We hypothesized that female mate selection should be more pronounced in the temperate J. edwardsii than in the tropical P. argus because J. edwardsii matures later, has a shorter mating season, and produces just one clutch of eggs per year that benefit from …

A Study Of A Simulated Infectious Disease On Healthcare Workers' Reaction, Knowledge, Attitudes, And Performance Towards Hand Hygiene, Lydia Wigglesworth-Ballard

Health Services Research Dissertations

Problem Statement. The high rate of healthcare worker-patient contact provides many opportunities for exposure to pathogens, which creates challenges in assessing healthcare workers' success at preventing healthcare associated infections caused by these dangerous pathogens.

Methods. This study explored the effects of a simulated infectious disease on healthcare workers' hand hygiene knowledge, attitudes, performance, and reactions using Kirkpatrick's Four levels of Evaluation. The study utilized a mixed method pre-test – 2 post-test design. The dependent variables were hand hygiene knowledge, attitudes toward hand hygiene guidelines, hand hygiene performance, and reaction to the overall experience. Data was collected three times over a …

Determining The Prevalence And Distribution Of Tick-Borne Pathogens In Southeastern Virginia And Exploring The Transmission Dynamics Of Rickettsia Parkeri In Amblyomma Maculatum, Chelsea L. Wright Thompson

Biological Sciences Theses & Dissertations

Tick-borne pathogens are an increasing threat to human and animal health worldwide. In the United States, cases of Lyme disease, spotted fever rickettsioses, ehrlichiosis and anaplasmosis are on the rise. Factors related to emergence include appearance of a new pathogen, recognition of an existing pathogen and environmental changes that result in new exposure events. Despite the rise in tick-borne disease incidence within many states, including Virginia, there is a paucity of data related to the prevalence and distribution of ticks and tick-borne pathogens.

The first aim of this dissertation research was to determine the tick-borne pathogen composition within tick populations …

Molecular Phylogeny Of The Genus Houstonia And Allies In Rubiaceae, Hunter Lee Shanks

Biological Sciences Theses & Dissertations

Houstonia (Rubiaceae) is a strictly North American genus of 24 species distributed from Mexico, throughout the United States, up to Canada. Houstonia has proven to be a taxonomically difficult genus since the Linnaean description of Houstonia and the related genera: Hedyotis and Oldenlandia in 1753. For over 250 years botanists have lumped and separated Houstonia from Hedyotis and Oldenlandia based on various morphological characters. The most recent circumscription of Houstonia (Terrell 1996) separated the genus into two subgenera with each subgenus containing two sections. Nuclear (ITS) and plastid (trnL-F, rps16) DNA sequences were used to build a molecular phylogeny depicting …

Perch Selection By Male Dragonflies (Odonata, Anisoptera) Related To Competitive Ability And Species Composition, Jessica L. Beard

Biological Sciences Theses & Dissertations

Males of many species of dragonflies (Odonata, Anisoptera) establish territories in aquatic habitats where they compete with other males for access to food and females. Territorial males typically perch on emergent vegetation and chase rival males who intrude into their territories. This dissertation research examined the role of male size in perch height selection, position on the perch, and competitive ability. Four hypotheses were tested: 1) Dragonfly species would vary by size and that territorial species would show sexual size dimorphism (SSD), 2) Perch height selection would be related to dragonfly size, 3) Position on the perch would be related …

Lower Extremity Biomechanics In Individuals With And Without Previous Hamstring Injury, Jessica A. Mutchler

Human Movement Sciences Theses & Dissertations

Primary risk factors for hamstrings strains such as fatigue, previous injury and strength deficits have been identified in the research, yet re-injury rates remain high among the physically active. Sub-acute analysis of the hip, knee and ankle biomechanics following a hamstrings strain have been largely overlooked and may provide additional insight into re-injury risks. It was the aim of this dissertation to explore long-term adaptations in walking and running tasks following a hamstrings strain, and to develop a hip endurance test that could be used in future studies.

Project one used a cross-sectional study with test-retest design to develop and …

The Effects Of Pre-Maximal Exertion Inhalation Of Ammonia And The Performance Effects During Deadlift Maximal Tests, Justin Nicholas Vigil

Human Movement Sciences Theses & Dissertations

The purpose of this study was to investigate the effects of ammonia as a stimulant on athletic performance during a deadlift one maximal repetition (1-RM) absolute strength test. It was hypothesized that ammonia inhalation would result in a larger 1-RM, possibly due to immediate catecholamine release attributed to the fight-or-flight response. If proven effective, ammonia inhalation would present an option to increase power and strength performance during training and competition based on an acute manipulation of natural occurring hormones, eliminating the side effects attributed to other supplementation methods. Subjects (n = 10 males, X±SD age = 21±1 years, mass = …

Behavioral Immunity Suppresses An Epizootic In Caribbean Spiny Lobsters, Mark J. Butler Iv, Donald C. Behringer Jr., Thomas W. Dolan Iii, Jessica Moss, Jeffrey D. Shields

Biological Sciences Faculty Publications

Sociality has evolved in a wide range of animal taxa but infectious diseases spread rapidly in populations of aggregated individuals, potentially negating the advantages of their social interactions. To disengage from the coevolutionary struggle with pathogens, some hosts have evolved various forms of "behavioral immunity"; yet, the effectiveness of such behaviors in controlling epizootics in the wild is untested. Here we show how one form of behavioral immunity (i.e., the aversion of diseased conspecifics) practiced by Caribbean spiny lobsters (Panulirus argus) when subject to the socially transmitted PaV1 virus, appears to have prevented an epizootic over a large seascape. We …

Isquest: Finding Insertion Sequences In Prokaryotic Sequence Fragment Data, Abhishek Biswas, David T. Gauthier, Desh Ranjan, Mohammad Zubair

Computer Science Faculty Publications

Motivation: Insertion sequences (ISs) are transposable elements present in most bacterial and archaeal genomes that play an important role in genomic evolution. The increasing availability of sequenced prokaryotic genomes offers the opportunity to study ISs comprehensively, but development of efficient and accurate tools is required for discovery and annotation. Additionally, prokaryotic genomes are frequently deposited as incomplete, or draft stage because of the substantial cost and effort required to finish genome assembly projects. Development of methods to identify IS directly from raw sequence reads or draft genomes are therefore desirable. Software tools such as Optimized Annotation System for Insertion Sequences …

Section Abstracts: Botany

Virginia Journal of Science

Abstracts of the Botany Section for the 93rd Annual Meeting of the Virginia Academy of Science, May 21-23, 2015, James Madison University, Richmond, Virginia

Section Abstracts: Agriculture, Forestry And Aquaculture

Virginia Journal of Science

Abstracts of the Agriculture, Forestry and Aquaculture Section for the 93rd Annual Virginia Academy of Science Meeting, May 2015, at James Madison University

Section Abstracts: Structural Biology, Biochemistry, And Biophysics

Virginia Journal of Science

Abstracts of the Structural Biology, Biochemistry, and Biophysics Section for the 93rd Annual Meeting of the Virginia Academy of Science, May 21-23, 2015, James Madison University, Richmond, Virginia

Section Abstracts: Biology With Microbiology And Molecular Biology

Virginia Journal of Science

Abstracts of the Biology with Microbiology and Molecular Biology Section for the 93rd Annual Meeting of the Virginia Academy of Science, May 21-23, 2015, James Madison University, Richmond, Virginia

Section Abstracts: Natural History And Biodiversity

Virginia Journal of Science

Abstracts of the Natural History and Biodiversity Section for the 93rd Annual Meeting of the Virginia Academy of Science, May 21-23, 2015, James Madison University, Richmond, Virginia