Life Sciences Commons^™

Evaluating The Role Of The Bovine Vaginal Microbiome In Neonatal And Maternal Health Outcomes, Riley D. Messman

Theses and Dissertations

The dam vaginal microbiota is the first major microbial inoculating community within the neonate. The composition of the dam vaginal microbiota has implications in calf commensal microbiota development. Alterations of the dam microbial community prior to parturition could alter inoculating communities and immune responses in both the dam and calf. Thus, authors aimed to elucidate the microbial community composition of the bovine dam vaginal and calf nasal microbiota post-partum after utilizing betadine lavages (BL). The dam vaginal and calf nasal microbial communities and immune responses were evaluated at 0-, 15-, 30- and 60-day post-partum. Microbiota composition of the dam haircoat, …

Isolation And Culture Of Epichloё For Reinfection Of Endophyte-Free Southeastern Wildrye (Elymus Glabriflorus), Bryna Joann Haile

Theses and Dissertations

Mutualistic relationships between endophytic fungi and grasses have shown to improve the hardiness of the host. This relationship is common in grasses, including North American native grasses that are important in both forage and grassland ecosystems. Elymus species, such as Canada wildrye (CWR), commonly host the endophytic fungi, Epichloё, while southeastern wildrye (SEWR) may not. In this study, seed of CWR and seed and leaves from local SEWR were assessed for endophyte infection. Infection status was confirmed via histological analysis of seed squashes and leaf peels. Presence/absence of endophyte was determined by scanning for mycelia within seed and between leaf …

Development Of Preservative-Treated Cross-Laminated Timber And Lignin-Reinforced Polyurethane-Adhesive For Glued Laminated Timber, Samuel Oluwafemi Ayanleye

Theses and Dissertations

Interest in the use of mass timber in building and construction is growing worldwide, this is due to the structural integrity and reduced environmental footprint of timber-based structures. Concerns associated with the biological and environmental degradation of mass timber necessitate the development of adequate protection strategies to ensure the durability of these products. Preservative treatment is a proven technique that increases the durability and performance of wood in-service and can also be applied to large-sized timber panels such as cross-laminated timber (CLT). Therefore, this study focused on investigating the feasibility of treating prefabricated 3- and 5-layer CLT panels with Copper-azole …

Regional Economic Impacts Of Enterococcus-Related Beach Closures In Mississippi, Jessica Lynn Browne

Theses and Dissertations

High levels of Enterococcus are the most frequent reason for beach closures on the Mississippi Gulf Coast and can be made worse by human activities. When beaches close, tourism can decrease, affecting sales. This study aims to 1) analyze the regional economic impact of beach closures at the county level, 2) show empirically how serious the impact of high levels of Enterococcus are compared to other causes of beach closures, and 3) discuss the policy implications for regional economies linked to beach closures. I use a response function approach with beach closure and county-level monthly gross taxable sales data. Results …

Impacts Of Cover Crop, Soil Steaming, And Plastic Mulch On Field-Grown Tomato Production And Virus-Induced Gene Silencing In Antirrhinum, Penstemon, Petunia, Rosa, And Rudbeckia, Brenton Andrew Earl Breland

Theses and Dissertations

Weeds and soil-borne diseases can cause large yield losses in field-grown tomato (Solanum lycopersicum) production. Techniques have been developed to reduce soil-based problems. In this study, we evaluated the impacts of cover crops, soil steaming, and plastic mulch to reduce weed and disease pressure in field-grown tomatoes. Four cover crop treatments were grown in the fall and winter before spring planting. Soils were steamed to a target temperature of 71.1 °C for 0, 5, or 20 minutes. Plastic mulch was also used on half of the rows. Yield, weed densities, and disease incidence were recorded.

Reduced flowering time …

A Comparison Of Ground Reaction Forces And Muscle Activity Of The Tsunami Bar® Against A Rigid Barbell During Back Squat Phases, John Carver Middleton

Theses and Dissertations

An Institutional Review Board (IRB)-approved study was conducted to investigate the effects of the Tsunami Bar® (TB), a flexible barbell, on ground reaction force (GRF) production and muscle activity in the quadricep, hamstring, and gluteal muscle groups during phases of the squat exercise and compare the effects to the effects to using a rigid barbell (RB). A two-by-two repeated measures Analysis of Variance (ANOVA) test was used to compare the results. Descriptive statistics showed significantly higher GRFs for the TB during the unweighting phase, significant differences in GRFs between speeds for each phase, significantly higher forces on average with the …

Host-Acquired Virus Genes Support An Ancient Antiviral Role Of The Pirna Pathway In Dipterans, Rebecca Christian

Theses and Dissertations

Endogenous viral elements (EVEs) have been recently investigated as a source of transgenerational immune memory. These “viral fossils” are abundant in Aedes mosquitoes and partner with the host’s primary antiviral defense system, the RNA interference (RNAi) pathways. This partnership appears unique to mosquitoes, which encode an expansion of the Piwi endoribonucleases. To interrogate EVE-Piwi partnerships and their role in antiviral defense, I performed a comparative small RNA analysis of two naturally occurring EVE-virus pairs – one in the mosquito Aedes albopictus, and one in the midge Chaoborus americanus. Both express an EVE related to the nucleoprotein of their …

Spatial Conservation Planning In The Southeastern United States: Alignments And Opportunities, Bradly Stewart Thornton

Theses and Dissertations

Conservation managers and planners need the ‘best available science’ to support robust and defensible decisions, ensuring that public resources are appropriately allocated. Spatial planning products and decision-support tools developed for this purpose should enable partner organizations to achieve focus, coordination, and increased effectiveness in their investments and actions. Whereas conservation partnerships have historically created distinct planning tools, there is increasing interest for improved coordination, communication, and unifying biological datasets to improve the cohesiveness of regional management activities. We sought to inform spatial conservation planning efforts in the southeastern United States through the development of species distribution models for focal avian …

Expanding The Market Of Biomaterials, Franklin Quin Jr.

Theses and Dissertations

Biomaterials such as wood and bamboo are in high demand as a building material with the push for building with green technology. The wood product industry accounts for approximately 4% of the total U.S. manufacturing GDP (Gross Domestic Product), which is more than $100 billion. The industry supports over 752,000 full-time equivalent jobs, most of which are in rural areas where employment opportunities are limited. The estimated global market value of bamboo is estimated to be $60 billion annually. This research will explore the use of wood and bamboo in different end use products. The objectives of this research will …

Understanding Dietary And Thermal Influences On Invasive Cichlids In Puerto Rico Reservoir Systems, Jacob A. Moreland

Theses and Dissertations

Jaguar Guapote (Parachromis managuensis) and Amphilophus spp. were assessed for effects of temperature on standard metabolic rate (SMR) and lower dissolved oxygen tolerance (LDOT), efficacy of gastric lavage for diet analysis, and diet in Puerto Rico reservoirs. Fish were acclimated to 22°C, 28°C, and 34°C and SMR and LDOT were measured using intermittent respirometers. Jaguar Guapote acclimated to 34°C had the greatest SMR, with 22°C and 28°C having similar SMR values. Amphilophus spp. acclimated to 28°C were similar to fish acclimated at 22°C and 34°C. Fish were resilient to high temperatures, losing equilibrium more rapidly at the coldest temperature, 22°C. …

Activity Level Of Helicoverpa Armigera Nucleopolyhedrovirus (Hearnpv) And Feeding Habits Of Helicoverpa Zea Larvae After Ingestion Of Hearnpv In Mississippi Soybean, Judge Tyler Fortenberry

Theses and Dissertations

HearNPV was applied to a soybean field and evaluated for residual efficacy at different time intervals (0, 12, 24, 36, 48, and 72 hours) after application in laboratory bioassays with treated leaves. Larval mortality was rated at five, seven and twelve days after larval infestation. Larval mortality increased as the number of days after infestation increased. To evaluate the feeding habits of Helicoverpa zea at the 2^nd and 3^rd instar, HearNPV exposed larvae and untreated larvae were weighed at zero (before diet exposure), four, and seven days. At four days after exposure, larval weight gain of …

Evaluation Of Italian Ryegrass And Palmer Amaranth Control In Mississippi, Johnson Harris Hughes

Theses and Dissertations

Italian ryegrass is a problematic weed in Mississippi corn production due to the development and proliferation of glyphosate resistance. Studies were conducted to assess Italian ryegrass control prior to planting using herbicides. Effects of fall and spring applied burndown herbicide applications for Italian ryegrass control and subsequent corn grain yield were investigated at the R.R. Foil PSRC in Starkville, MS, at the Coastal Plain Experiment Station in Newton, MS, and the Black Belt Experiment Station in Brooksville, MS on soil textures ranging from sandy loam to silt clay loam. A fall preemergence (PRE) application of S-metolachlor + metribuzin followed …

Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari

Theses and Dissertations

This study identified marginal agricultural lands in the Lower Mississippi Alluvial Valley using crop yield predicting models. The Random Forest Regression (RFR) and Multiple Linear Regression (MLR) models were trained and validated using county-level crop yield data, climate data, soil properties, and Normalized Difference Vegetation Index (NDVI). The RFR model outperformed MLR model in estimating soybean and corn yields, with an index of agreement (d) of 0.98 and 0.96, Nash-Sutcliffe model efficiency (NSE) of 0.88 and 0.93, and root mean square error (RMSE) of 9.34% and 5.84%, respectively. Marginal agricultural lands were estimated to 26,366 hectares using cost and sales …

Modeling Yield And Aboveground Live Tree Carbon Dynamics In Oak-Gum-Cypress Bottomland Hardwood Forests, Suchana Aryal

Theses and Dissertations

The importance of bottomland hardwood (BLH) forests to support the economy through timber production and carbon sequestration is acknowledged; however, their full potential is yet to be explored. This study developed variable density yield models for BLH oak-gum-cypress forests along the US Gulf Coast and Lower Mississippi River Delta. The models, with an adjusted R2 of 98% for cubic foot growing stock volume and 77% for Doyle board foot sawlog volume, are expected to be valuable tools for landowners and managers seeking to make informed decisions about BLH forest management.

A carbon stock model was also developed, and carbon sequestration …

Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco

Theses and Dissertations

The Amazon River drainage basin is known as the most biodiverse region in the world. Regarding freshwater fish, this region contains around 15% of the world's fish richness. The fish family Serrasalmidae have a variety of feeding preferences including Pacus (herbivores) and Piranhas (carnivores). Fruit eating fish species are evolutionarily the oldest species in the family and have a mutualistic interaction with flooded forests. Considering the diversity of feeding habits of the Serrasalmidae family, it represents an excellent model to study ecological questions related to factors that influence the spatial distribution of species and factors that contribute to the maintenance …

Managing Iron Deficiency Chlorosis (Idc) In Soybean Through A Cropping System Approach, Katelin Savannah Waldrep

Theses and Dissertations

Iron deficiency chlorosis (IDC) is a frequent problem throughout many areas of the United States where soils are high in calcium carbonate (CaCO₃), including the Blackland Prairie regions of Mississippi. The main objectives of this study were to 1) determine the effects of seven different cropping systems on IDC visual symptomology and grain yield in rainfed soybeans grown in calcareous soils, and 2) evaluate the effects of soil water tension (SWT) on IDC. Rotating soybeans with corn produced significantly higher yields for both tolerant and susceptible soybean varieties. IDC symptomology was worse, and yields were lower in cropping …

Examining Physiological And Photosynthetic Effect Of Led Lighting On Lettuce And Kale Microgreens, James Travis Blanche

Theses and Dissertations

The objective of these studies was to observe the effects of LED lighting treatments on physiological parameters of lettuce and microgreens. Four different lighting treatments; 1) Physiospec (white), 2) Red, 3) DR9 (25:75 Blue:Red), and 4) Anthospec (at 20/80 Blue:Red); were used in this experiment. Two experiments were conducted: 1) Examining the effects of LED lights on lettuce yield and photosynthetic activity and 2) The effects of switching lighting regiments halfway through the growth cycle on Kale microgreen yield and color. Physiospec lighting was found to promote the highest yield and photosynthetic activity among the four treatments for lettuce. It …

Genome-Wide Association Study For Agronomic Traits In Bermudagrass (Cynodon Spp.), Lovepreet Singh

Theses and Dissertations

Bermudagrass (Cynodon spp.) breeding and cultivar development is hampered by limited information regarding its genetic and phenotypic diversity. A germplasm collection of 206 bermudagrass accessions from 29 countries was genotyped with high-throughput genotyping-by-sequencing technique. Genomic diversity in this diverse germplasm panel was assessed with multifaceted approaches including population structure, phylogenetic analysis, principal component analysis, and genetic diversity parameters. This study revealed substantial genetic variation in the Cynodon accessions, demonstrating the potential of this germplasm panel for further genetic studies and cultivar development in breeding programs. Another critical issue in turfgrass breeding is the lack of information regarding the genetic …

The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel

Theses and Dissertations

The Intergovernmental Panel on Climate Change has predicted that there will be increases in precipitation and heat-induced drought events globally. Information on kale response to waterlogging is minimal. The purpose of this project was to identify the response of kale to three treatments of water stress: underwatering, overwatering, and waterlogging. Plant pigments analyzed displayed a varied response to underwatering and overwatering, with concentrations changing with maturity but with reductions and no changes, respectively, at full harvest maturity. Glucosinolate concentrations were also influenced by maturity and increased under waterlogging and underwatering but no differences with overwatering. Overall, water stress to any …

The Response Of An Avian Community To Intercropping And Forest Management Practices In A Private Working Pine Forest, Rebecca Doyne Bracken

Theses and Dissertations

Within managed pine forest systems, a plethora of bird species exist throughout the lifecycle of a stand akin to what may be experienced through post-disturbance regeneration in a natural forest system. I sought to address how breeding avian communities shift across time in response to stand aging and forest management, evaluate species-specific responses to stand conditions, investigate the responses of at-risk avian species to forest management, and determine avian non-breeding, over-wintering presence in a managed loblolly pine (Pinus taeda) forest. I conducted breeding bird point count and vegetation surveys within five stands of privately owned and managed pine forest in …

Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith

Theses and Dissertations

The importance of the gut microbiome is being explored in relation to multiple facets of health and performance. The purpose of this study was to explore the impact of frequent sweet potato ingestion on alterations of the gut microbiome which might in turn alter cholesterol levels and exercise performance. Thirteen recreationally trained males had fecal and blood samples collected and exercised to fatigue with and without exogenous carbohydrate ingestion prior to and following 42 days of daily sweet potato ingestion. Bacterial communities were extracted from fecal samples and bacterial DNA were sequence. Blood samples were analyzed for total cholesterol, low-density …

Role Of Fungal Arv-1 Protein In Sterol Metabolism And Pathogenicity Of The Chestnut Blight Fungus Cryphonectria Parasitica, Soumyadip Kundu

Theses and Dissertations

Intracellular sterol redistribution is an important step in the lipid homeostasis of organisms, a process directly linked to the organizational arrangement in the plasma membrane (PM) of cells. Previous studies in the budding yeast Saccharomyces cerevisiae have demonstrated that the ARV1 (ACAT-related enzyme-2 required for viability 1) protein is a major regulator of sterol transport from the endoplasmic reticulum to the plasma membrane, contributing to the structural organization of the PM, rendering it resistant to anti-fungal compounds as well as maintaining ER integrity. This study assessed the significance of ARV1 in the plant pathogenic fungus Cryphonectria parasitica (Cparv1) …

Determining The Impact Of Post-Harvest Water Management On Chironomid Abundance, Agrochemical Biomass And Potential Trophic Biomagnification, Mason Thomas

Theses and Dissertations

Agriculture has diminished shorebirds’ natural habitat in the Mississippi Alluvial Valley. Remaining natural stopover sites are supplemented with agricultural fields during the fall and winter. This study evaluates the impact of 4 different post-harvest water management strategies on shorebird food abundance and potential agrochemical biomagnification. Chironomid samples estimated abundance, biomass, and chironomid agrochemical concentration in each field. A risk assessment of agrochemical biomagnification to shorebirds was made across all treatments. Of treatments represented on all study sites, winter treatment had greatest chironomid abundance and biomass. Models indicated that days since flood initiation, start date, and temperature are significant predictors of …

Evaluation Of The Relationship Between Hair Coat Shedding Ability, Apparent Forage Digestibility, And Mineral Status In Angus Cattle, Margaret P. Loftin

Theses and Dissertations

The objective of this study was to evaluate the relationship between hair coat shedding ability, apparent forage digestibility, and mineral status in Angus cattle. Fecal samples were collected on fall calving Angus cows for one week in March, May, and July from 2019-2022. Liver and plasma samples were collected in March and July each year. Fecal samples were analyzed for DM, ash, NDF, and ADF while liver and plasma were analyzed for mineral concentration. Data were analyzed using MIXED procedure of SAS with significance declared at P0.05. Interactions were detected for DM, NDF, ADF, and liver Fe concentration. The model …

Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland

Theses and Dissertations

Organophosphates are insecticides which result in acute adverse signs when exposed at toxic doses by animals and lead to death if left untreated. The current treatment for organophosphate toxicity includes atropine and the federally approved oxime 2-PAM. However, 2-PAM is not very effective at crossing the blood brain barrier which results in prolonged inactivation of acetylcholinesterase, which is the primary target of organophosphates, in the brain even after administration. The novel oximes, Oxime 15 and Oxime 20, are able to cross the blood brain barrier and reactivate the inhibited acetylcholinesterase. In this experiment with six animal species frequently used in …

Evaluation Of Early Maturing Cultivars, Optimal Harvest Timing, And Canopy Reflectance Of Peanut To Maximize Grade And Yield, Joseph Bryan Whittenton

Theses and Dissertations

Peanut digging timing is difficult to predict due to indeterminate growth and peanut pods maturing underground, resulting in the need to research methods that provide consistent measurements, while reducing time and effort for farmers and researchers. Experiments were conducted to evaluate the accuracy of the Maturity Index 1 and Maturity Index 2 in predicting peanut grade, the accuracy of the North Carolina 2 degree day method in predicting peanut yield, and remote sensing vegetative indices sensitivity equivalence (SEq) to peanut Maturity Index 2 and harvest grade (TSMK) for cultivars IPG-914 and Georgia-06G in Mississippi.

Maturity Index 1 and …

Determining The Importance Of Propagule Pressure And Dispersal Mechanisms For The Establishment And Spread Of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae Kuwana (Hemiptera: Eriococcidae), Erika Renee Wright

Theses and Dissertations

Non-native scale insects can be economically and ecologically important pests of urban forests. Crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae Kuwana (Hemiptera: Eriococcidae), is a scale that causes declines in plant vigor for a popular ornamental tree in the southeastern United States, crapemyrtle (Lagerstroemia spp.). CMBS has spread rapidly throughout the Southeast and, more recently, into the Middle Atlantic. Despite problems associated with CMBS, important aspects of its ecology, such as the role of propagule pressure in establishment and the relative importance of mechanisms for between-tree dispersal, are not well-understood. We used field and laboratory studies to investigate these aspects of …

Physiology Of Human-Equine Interaction During Substance Withdrawal, Molly Marie Friend

Theses and Dissertations

Psychotherapy incorporating equine interaction (PIE) is emerging as an effective treatment for substance use disorder (SUD), but research is limited concerning physiological impacts during substance withdrawal. This study investigated impacts of PIE on salivary cortisol concentrations, heart rate, anxiety, and depression during SUD withdrawal. Heart rate and cortisol concentrations were measured in horses to determine potential human-horse coupling. Saliva samples and heart rates were collected pre and post PIE from residential SUD patients (n=18) and their therapy horses (n=4) during and following the withdrawal period. Participants (n=10) also completed a survey measuring anxiety and depression (P = 0.05). A …

Surface Modification Of Wood Using Nano-Sized Titania Particles Coated By Liquid-Precursor Flame Spray Pyrolysis, Ganesh Sedhain

Theses and Dissertations

Wood is a renewable resource and versatile material used in tasks ranging from tools and furniture to advanced engineering structures. Although wood is light, mechanically robust, environmentally friendly, and abundant, some inherent properties of wood, such as degradation due to moisture and UV radiation from sunlight, are less desirable for extended service life and dimensional stability. In this dissertation, a novel surface modification of wood is explored by depositing nano-sized titania particles on wood veneers and cross-laminated timber (CLT) blocks by liquid-precursor flame spray pyrolysis to confer reversible wettability switching and enhanced durability to UV irradiation. The reaction between a …

Evolution And Epidemiology Of Channel Catfish Virus (Ccv), Arun Venugopalan

Theses and Dissertations

Channel catfish virus disease (CCVD) is the principal viral disease in the United States catfish industry. The CCVD is caused by channel catfish virus (CCV), with mortality reaching up to 100% in fingerlings. CCV is assigned taxonomically to the family Alloherpesviridae, genus Ictalurivirus, species Ictalurid herpesvirus 1 (IcHV-1). To date, virulence, immunogenicity, and genome plasticity of the CCV field isolates have not been investigated. Three genotypes of CCV (IcHV-1A, IcHV-1B, and BCAHV) were identified using restriction fragment length polymorphism (RFLP) analysis. Virulence assessment of three representative isolates of RFLP groups suggests that IcHV-1B (pooled survival [mean ± SE]: 58.3% ± …