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Full-Text Articles in Life Sciences
Repeated Vaginal Exposures To The Common Cosmetic And Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain In Nd4 Mice, Erica Arriaga-Gomez, Jacyln Kline, Elizabeth Emanuel, Nefeli Neamonitaki, Tenzin Yangdon, Hayley Zacheis, Dogukan Pasha, Jinyoung Lim, Susan Bush, Beebie Boo, Hanna Mengistu, Ruby Kinnamon, Robin Shields-Cutler, Wattenberg Wattenberg, Devavani Chatterjea
Repeated Vaginal Exposures To The Common Cosmetic And Household Preservative Methylisothiazolinone Induce Persistent, Mast Cell-Dependent Genital Pain In Nd4 Mice, Erica Arriaga-Gomez, Jacyln Kline, Elizabeth Emanuel, Nefeli Neamonitaki, Tenzin Yangdon, Hayley Zacheis, Dogukan Pasha, Jinyoung Lim, Susan Bush, Beebie Boo, Hanna Mengistu, Ruby Kinnamon, Robin Shields-Cutler, Wattenberg Wattenberg, Devavani Chatterjea
Faculty Publications
A history of allergies doubles the risk of vulvodynia-a chronic pain condition of unknown etiology often accompanied by increases in numbers of vulvar mast cells. We previously established the biological plausibility of this relationship in mouse models where repeated exposures to the allergens oxazolone or dinitrofluorobenzene on the labiar skin or inside the vaginal canal of ND4 Swiss Webster outbred mice led to persistent tactile sensitivity and local increases in mast cells. In these models, depletion of mast cells alleviated pain. While exposure to cleaning chemicals has been connected to elevated vulvodynia risk, no single agent has been linked to …
Tetrahydrocannabinol Reduces Hapten-Driven Mast Cell Accumulation And Persistent Tactile Sensitivity In Mouse Model Of Allergen-Provoked Localized Vulvodynia, Beebie Boo, Rohit Kamath, Erica Arriaga-Gomez, Jasmine Landry, Elizabeth Emanuel, Sookyong Joo, Marietta Sald Montivero, Tijana Martinov, Brian T. Fife, Devavani Chatterjea
Tetrahydrocannabinol Reduces Hapten-Driven Mast Cell Accumulation And Persistent Tactile Sensitivity In Mouse Model Of Allergen-Provoked Localized Vulvodynia, Beebie Boo, Rohit Kamath, Erica Arriaga-Gomez, Jasmine Landry, Elizabeth Emanuel, Sookyong Joo, Marietta Sald Montivero, Tijana Martinov, Brian T. Fife, Devavani Chatterjea
Faculty Publications
Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. An increase in numbers of vulvar mast cells often accompanies a clinical diagnosis of vulvodynia and a history of allergies amplifies the risk of developing this condition. We previously showed that repeated exposures to oxazolone dissolved in ethanol on the labiar skin of mice led to persistent genital sensitivity to pressure and a sustained increase in labiar mast cells. Here we sensitized female mice to the hapten dinitrofluorobenzene (DNFB) dissolved in saline on their flanks, and subsequently challenged them with the same hapten or saline vehicle alone for ten …
Variation In Mating Dynamics Across Five Species Of Leiobunine Harvestmen (Arachnida: Opliones), Kasey D. Fowler-Finn, Sarah L. Boyer, Raine Ikagawa, Timothy Jeffries, Penelope C. Kahn, Eva M. Larsen, Daniel Lee, Morgan Smeester
Variation In Mating Dynamics Across Five Species Of Leiobunine Harvestmen (Arachnida: Opliones), Kasey D. Fowler-Finn, Sarah L. Boyer, Raine Ikagawa, Timothy Jeffries, Penelope C. Kahn, Eva M. Larsen, Daniel Lee, Morgan Smeester
Faculty Publications
No abstract provided.
Nuptial Gift Chemistry Reveals Convergent Evolution Correlated With Antagonism In Mating Systems Of Harvestmen (Arachnida, Opiliones), Penelope C. Kahn, Dennis D. Cao, Mercedes Burns, Sarah L. Boyer
Nuptial Gift Chemistry Reveals Convergent Evolution Correlated With Antagonism In Mating Systems Of Harvestmen (Arachnida, Opiliones), Penelope C. Kahn, Dennis D. Cao, Mercedes Burns, Sarah L. Boyer
Faculty Publications
Nuptial gifts are material donations given from male to female before or during copulation and are subject to sexual selection in a wide variety of taxa. The harvestman genus Leiobunum has emerged as a model system for understanding the evolution of reproductive morphology and behavior, as transitions between solicitous and antagonistic modes of courtship have occurred multiple times within the lineage and are correlated with convergence in genital morphology. We analyzed the free amino acid content of nuptial gift secretions from five species of Leiobunum using gas chromatography–mass spectrometry. Multivariate analysis of the free amino acid profiles revealed that, rather …
Repeated Hapten Exposure Induces Persistent Tactile Sensitivity In Mice Modeling Localized Provoked Vulvodynia, Tijana Martinov, Jasmine Landry, Hanna Mengistu, Jyothi Dhanwada, Charles J. Benck, Jacyln Kline, Beebie Boo, Linnea Swanson, Elena Tonc, Randy Daughters, Brian T. Fife, Devavani Chatterjea
Repeated Hapten Exposure Induces Persistent Tactile Sensitivity In Mice Modeling Localized Provoked Vulvodynia, Tijana Martinov, Jasmine Landry, Hanna Mengistu, Jyothi Dhanwada, Charles J. Benck, Jacyln Kline, Beebie Boo, Linnea Swanson, Elena Tonc, Randy Daughters, Brian T. Fife, Devavani Chatterjea
Faculty Publications
BACKGROUND:
Vulvodynia is a remarkably prevalent chronic pain condition of unknown etiology. Epidemiologic studies associate the risk of vulvodynia with a history of atopic disease. We used an established model of hapten-driven contact hypersensitivity to investigate the underlying mechanisms of allergy-provoked prolonged sensitivity to pressure.
METHODS:
We sensitized female ND4 Swiss mice to the hapten oxazolone on their flanks, and subsequently challenged them four days later with oxazolone or vehicle for ten consecutive days on the labia. We evaluated labiar sensitivity to touch, local mast cell accumulation, and hyperinnervation after ten challenges.
RESULTS:
Oxazolone-challenged mice developed significant tactile sensitivity that …
Measuring Changes In Tactile Sensitivity In The Hind Paw Of Mice Using An Electronic Von Frey Apparatus, Tijana Martinov, Madison Mack, Akilah Sykes, Devavani Chatterjea
Measuring Changes In Tactile Sensitivity In The Hind Paw Of Mice Using An Electronic Von Frey Apparatus, Tijana Martinov, Madison Mack, Akilah Sykes, Devavani Chatterjea
Faculty Publications
Measuring inflammation-induced changes in thresholds of hind paw withdrawal from mechanical pressure is a useful technique to assess changes in pain perception in rodents. Withdrawal thresholds can be measured first at baseline and then following drug, venom, injury, allergen, or otherwise evoked inflammation by applying an accurate force on very specific areas of the skin. An electronic von Frey apparatus allows precise assessment of mouse hind paw withdrawal thresholds that are not limited by the available filament sizes in contrast to classical von Frey measurements. The ease and rapidity of measurements allow for incorporation of assessment of tactile sensitivity outcomes …
Tnf-Alpha Neutralizing Antibody Blocks Thermal Sensitivity Induced By Compound 48/80-Provoked Mast Cell Degranulation, Devavani Chatterjea, Luisa Paredes, Tijana Martinov, Evelyn Balsells, Juliann Allen, Akilah Sykes, Alyssa Ashbaugh
Tnf-Alpha Neutralizing Antibody Blocks Thermal Sensitivity Induced By Compound 48/80-Provoked Mast Cell Degranulation, Devavani Chatterjea, Luisa Paredes, Tijana Martinov, Evelyn Balsells, Juliann Allen, Akilah Sykes, Alyssa Ashbaugh
Faculty Publications
BACKGROUND:
Neuro-inflammatory circuits in the tissue regulate the complex pathophysiology of pain. Protective nociceptive pain serves as an early warning system against noxious environmental stimuli. Tissue-resident mast cells orchestrate the increased thermal sensitivity following injection of basic secretagogue compound 48/80 in the hind paw tissues of ND4 mice. Here we investigated the effects of pre-treatment with TNF-α neutralizing antibody on compound 48/80-provoked thermal hyperalgesia.
METHODS:
We treated ND4 Swiss male mice with intravenous anti-TNF-α antibody or vehicle 30 minutes prior to bilateral, intra-plantar compound 48/80 administration and measured changes in the timing of hind paw withdrawal observed subsequent to mice …
Contact Hypersensitivity To Oxazolone Provokes Vulvar Mechanical Hyperalgesia In Mice, Tijana Martinov, Rose Glenn-Finer, Sarah Burley, Elena Tonc, Evelyn Balsells, Alyssa Ashbaugh, Linnea Swanson, Randy S. Daughters, Devavani Chatterjea
Contact Hypersensitivity To Oxazolone Provokes Vulvar Mechanical Hyperalgesia In Mice, Tijana Martinov, Rose Glenn-Finer, Sarah Burley, Elena Tonc, Evelyn Balsells, Alyssa Ashbaugh, Linnea Swanson, Randy S. Daughters, Devavani Chatterjea
Faculty Publications
The interplay among pain, allergy and dysregulated inflammation promises to yield significant conceptual advances in immunology and chronic pain. Hapten-mediated contact hypersensitivity reactions are used to model skin allergies in rodents but have not been utilized to study associated changes in pain perception in the affected skin. Here we characterized changes in mechanical hyperalgesia in oxazolone-sensitized female mice challenged with single and repeated labiar skin exposure to oxazolone. Female mice were sensitized with topical oxazolone on their flanks and challenged 1-3 times on the labia. We then measured mechanical sensitivity of the vulvar region with an electronic pressure meter and …