Life Sciences Commons^™

Pd-L1 Expression On Circulating Tumor Cells May Be Predictive Of Response To Pembrolizumab In Advanced Melanoma: Results From A Pilot Study, Muhammad K. Khattak, Anna L. Reid, James Freeman, Michelle Pereira, Ashleigh Mcevoy, Johnny Lo, Markus Frank, Tarek Meniawy, Ali Didan, Isaac Spencer, Benhur Amanuel, Michael Millward, Mel Ziman, Elin Gray

Research outputs 2014 to 2021

BACKGROUND: PD-1 inhibitors are routinely used for the treatment of advanced melanoma. This study sought to determine whether PD-L1 expression on circulating tumor cells (CTCs) can serve as a predictive biomarker of clinical benefit and response to treatment with the PD-1 inhibitor pembrolizumab.

METHODS: Blood samples were collected from patients with metastatic melanoma receiving pembrolizumab, prior to treatment and 6-12 weeks after initiation of therapy. Multiparametric flow cytometry was used to identify CTCs and evaluate the expression of PD-L1.

RESULTS: CTCs were detected in 25 of 40 patients (63%). Patients with detectable PD-L1

CONCLUSION: Our results reveal the potential of …

Environmental Factors Influencing Primary Productivity Of The Forest-Forming Kelp Laminaria Hyperborea In The Northeast Atlantic, Dan A. Smale, Albert Pessarrodona, Nathan King, Michael T. Burrows, Anna Yunnie, Thomas Vance, Pippa Moore

Research outputs 2014 to 2021

© 2020, The Author(s). Rates and drivers of primary productivity are well understood for many terrestrial ecosystems, but remain poorly resolved for many marine ecosystems, particularly those within in coastal benthic environments. We quantified net primary productivity (NPP) using two methods as well as carbon standing stock within kelp forests (Laminaria hyperborea) at multiple subtidal habitats in the United Kingdom (UK). Study sites spanned 9° in latitude and encompassed a gradient in average temperature of ~ 2.5 °C. In addition to temperature, we measured other factors (e.g. light intensity, water motion, nutrients, sea urchin density) that may influence productivity. Although …

Conserving Critical Seagrass Habitat For Dugong. Dugong Seagrass Associations Summary Data., Kathryn M. Mcmahon, Paul Lavery, Nicole Said, Chris Cleguer, Amanda Hodgson

Research Datasets

This is the summary dataset of the dugong seagrass associations data containing dugong counts, benthic habitat cover, seagrass nutrient content, water temperature, water current speed, water depth and water transparency from three locations in the Pilbara over 3 time periods in 2018-2019.

Conserving Critical Seagrass Habitat For Dugong. Seagrass Network Monitoring Data Summary, Kathryn M. Mcmahon, Paul Lavery, Nicole Said

Research Datasets

This is the summary dataset of the seagrass monitoring data containing benthic habitat, seagrass meadow characteristics, seed density and environmental variables from three locations in the Pilbara over 3-4 time periods in 2018-2019.

Predictors Of Marine Genetic Structure In The Indo-Australian Archipelago [Dataset], Kathryn M. Mcmahon, Udhi Eko Hernawan

Research Datasets

This is the dataset extracted from 101 publications on 99 marine species from eight taxonomic groups in the Indo Australian Archipelago. Data extracted includes measures of genetic structure and the potential predictors.

Challenges To Select Suitable Habitats And Demonstrate ‘Additionality’ In Blue Carbon Projects: A Seagrass Case Study, Anna Lafratta, Oscar Serrano, Pere Masqué, Miguel A. Mateo, Milena Fernandes, Sam Gaylard, Paul S. Lavery

Research outputs 2014 to 2021

© 2020 Elsevier Ltd Seagrass restoration has been suggested as a Blue Carbon (BC) strategy for climate change mitigation. For Nationally Determined Contributions (NDC) and carbon crediting schemes, BC projects need to demonstrate ‘additionality’, that is enhanced CO2 sequestration and/or avoided greenhouse gas emissions following management actions. This typically requires determining soil carbon accumulation rates (CAR), which is often done using radionuclides or surface elevation tables to estimate sedimentation rates. Here we undertook a case study, using 210Pb and 14C dating, to detect possible changes in Corg stocks and CAR following the loss and partial recovery of Posidonia seagrass meadows …

Global Patterns In Seagrass Herbivory: Why, Despite Existing Evidence, There Are Solid Arguments In Favor Of Latitudinal Gradients In Seagrass Herbivory, K. L. Heck Jr, M. Samsonova, A. G. B. Poore, Glenn A. Hyndes

Research outputs 2014 to 2021

© 2020, Coastal and Estuarine Research Federation. The ecological paradigm that biological interactions are more intense in the tropics than in temperate or polar regions has existed since the mid-twentieth century, but several recent meta-analyses have provided scant evidence for latitudinal gradients in the intensity of herbivory. This contradictory evidence led us to carefully review the data and results of several of those papers that failed to find latitudinal gradients in rates of seagrass herbivory. To re-evaluate the arguments around the presence or absence of latitudinal gradients in herbivory in seagrass, we began by expanding the selection criteria to include …

Pharmacokinetics And Antiangiogenic Studies Of Potassium Koetjapate In Rats, Seyedeh F. Jafari, Fouad Saleih R. Al-Suede, Ashwaq H.S. Yehya, Mohamed B.K. Ahamed, Armaghan Shafaei, Muhammad Asif, Yasser M. Tabana, Amin M.S.A. Majid, Hussein M. Baharetha

Research outputs 2014 to 2021

© 2020 Purpose: Koetjapic acid is an active compound of a traditional medicinal plant, Sandoricum koetjape. Although koetjapic acid has a promising anticancer potential, yet it is highly insoluble in aqueous solutions. To increase aqueous solubility of koetjapic acid, we have previously reported a chemical modification of koetjapic acid to potassium koetjapate (KKA). However, pharmacokinetics of KKA has not been studied. In this study, pharmacokinetics and antiangiogenic efficacy of KKA are investigated. Methods: Pharmacokinetics of KKA was studied after intravenous and oral administration in SD rats using HPLC. Anti-angiogenic efficacy of KKA was investigated in rat aorta, human endothelial cells …

Human Impact Overrides Bioclimatic Drivers Of Red Fox Home Range Size Globally, Michael T. Main, Robert A. Davis, David Blake, Harriet Mills, Tim S. Doherty

Research outputs 2014 to 2021

© 2020 The Authors. Diversity and Distributions published by John Wiley & Sons Ltd Aim: Identifying the variables that influence animal home range size is important for understanding the biological requirements of individuals and their social interactions. Given their often broad distributions, carnivores are model organisms for studying range-wide determinants of home range size. Here, we test predictions about environmental determinants of home range size for one of the world's most widely distributed carnivores, the red fox (Vulpes vulpes). Location: Global. Methods: We compiled a database of 70 mean home range estimates from 62 studies and four continents, which we …

Deciphering The Unique Structure And Acylation Pattern Of Posidonia Oceanica Lignin, Jorge Rencoret, Gisela Marques, Oscar Serrano, Joeri Kaal, Angel T. Martínez, José C. Del Río, Ana Gutiérrez

Research outputs 2014 to 2021

Copyright © 2020 American Chemical Society. Lignins from different parts of the seagrass Posidonia oceanica-namely sheaths, rhizome, and roots-as well as from fibrous balls from P. oceanica detritus were isolated and thoroughly characterized by pyrolysis coupled with gas chromatography/mass spectrometry, derivatization followed by reductive cleavage, two-dimensional nuclear magnetic resonance spectroscopy, and gel permeation chromatography. The lignins of P. oceanica were enriched in guaiacyl (G) over syringyl (S) units, with S/G ratios ranging from 0.1 (fibrous balls) to 0.5 (rhizome). β-O-4′ ethers and phenylcoumarans were the most abundant lignin substructures, followed by resinols, and minor amounts of dibenzodioxocins and spirodienones. Moreover, …

A Step Forward Towards Advanced And Self-Sustainable Greenhouse Agriculture, Mohammad Nur E Alam, Mikhail Vasiliev, Jacqualine Anne Thomas

Research outputs 2014 to 2021

It is now time for the future-generation and advanced greenhouse design practices to address a range of issues, from the energy and land use efficiency to providing plant-optimised growth techniques. In this Encyclopaedia record, we report on the practical development of spectrally selective and specialist-type advanced metal-dielectric thin-film filters that produce the optimized illumination spectrum when exposed to natural sunlight that can help maximize the biomass productivity of coated-glass greenhouse crops. Our experimental case study has been performed for the lettuce species, Lactuca sativa, L., yielding promising results.

Material Residence Time In Marine Canopies Under Wave-Driven Flows, Maryam Abdolahpour, Marco Ghisalberti, Kathryn Mcmahon, Paul Lavery

Research outputs 2014 to 2021

© Copyright © 2020 Abdolahpour, Ghisalberti, McMahon and Lavery. Coastal canopies (e.g., seagrasses, coral reefs, and kelp forests) are vitally important ecosystems that provide a range of ecological services (e.g., oxygen production, sediment stabilization and trapping, and recycling of nutrients). The long-term health, productivity, and survival of these canopies rely heavily on the residence time of ecologically-significant materials in these environments. Recent studies have shown that submerged canopies induce a strong mean current over the canopy top, even in purely wave-dominated environments. Thus, in addition to vertical mixing, the horizontal flushing of materials (resulting from these canopy-induced currents) will dictate …

Seasonal Variation Of Bacterial Diversity Along The Marine Particulate Matter Continuum, Mireia Mestre, Juan Höfer, M. Montserrat Sala, Josep M. Gasol

Research outputs 2014 to 2021

© Copyright © 2020 Mestre, Höfer, Sala and Gasol. Seasonal dynamics of ocean prokaryotic communities in the free-living fraction have been widely described, but less is known about the seasonality of prokaryotes inhabiting marine particles. We describe the seasonality of bacterial communities in the particulate matter continuum by sampling monthly over two years in a temperate oligotrophic coastal ecosystem and using a serial filtration (including six size-fractions spanning from 0.2 to 200 μm). We observed that bacterial communities in the particulate matter continuum had annual changes following harmonic seasonal oscillations, where alpha, beta, and gamma diversity increased during the warm …

Review Of The Scientific And Institutional Capacity Of Small Island Developing States In Support Of A Bottom-Up Approach To Achieve Sustainable Development Goal 14 Targets, Rebecca Zitoun, Sylvia G. Sander, Pere Masque Barri, Saul Perez Pijuan, Peter W. Swarzenski

Research outputs 2014 to 2021

Capacity building efforts in Small Island Developing States (SIDS) are indispensable for the achievement of both individual and collective ocean-related 2030 agenda priorities for sustainable development. Knowledge of the individual capacity building and research infrastructure requirements in SIDS is necessary for national and international efforts to be effective in supporting SIDS to address nationally-identified sustainable development priorities. Here, we present an assessment of human resources and institutional capacities in SIDS United Nations (UN) Member States to help formulate and implement durable, relevant, and effective capacity development responses to the most urgent marine issues of concern for SIDS. The assessment highlights …

Review Of Coast And Marine Ecosystems In Temperate Australia Demonstrates A Wealth Of Ecosystem Services, Sam Gaylard, Michelle Waycott, Paul Lavery

Research outputs 2014 to 2021

© Copyright © 2020 Gaylard, Waycott and Lavery. Temperate Australia has extensive and diverse coast and marine habitats throughout its inshore and offshore waters. The region includes the southernmost extent of mangroves, over 500 estuaries and coastal embayments, home to extensive meadows of seagrasses and tidal saltmarsh. In areas of hard substrate, rocky reefs are abundant and productive with large forests of macroalgae. Coastal regions can be densely populated by humans and often habitats can be degraded, polluted or lost, while some remain relatively isolated and pristine. These habitats provide services to society including provision of food, regulate our climate …

Estimating The Potential Blue Carbon Gains From Tidal Marsh Rehabilitation: A Case Study From South Eastern Australia, Anne Gulliver, Paul E. Carnell, Stacey M. Trevathan-Tackett, Micheli Duarte De Paula Costa, Pere Masqué, Peter I. Macreadie

Research outputs 2014 to 2021

© Copyright © 2020 Gulliver, Carnell, Trevathan-Tackett, Duarte de Paula Costa, Masqué and Macreadie. Historically, coastal “blue carbon” ecosystems (tidal marshes, mangrove forests, seagrass meadows) have been impacted and degraded by human intervention, mainly in the form of land acquisition. With increasing recognition of the role of blue carbon ecosystems in climate mitigation, protecting and rehabilitating these ecosystems becomes increasingly more important. This study evaluated the potential carbon gains from rehabilitating a degraded coastal tidal marsh site in south-eastern Australia. Tidal exchange at the study site had been restricted by the construction of earthen barriers for the purpose of reclaiming …

Impacts Of Invasive Rats And Tourism On A Threatened Island Bird: The Palau Micronesian Scrubfowl, Paul M. Radley, Robert A. Davis, Tim S. Doherty

Research outputs 2014 to 2021

Invasive predators have decimated island biodiversity worldwide. Rats (Rattus spp.) are perhaps the greatest conservation threat to island fauna. The ground nesting Palau Micronesian Scrubfowl Megapodius laperouse senex (Megapodiidae) inhabits many of the islands of Palau's Rock Island Southern Lagoon Conservation Area (RISL) in the western Pacific. These islands are also heavily visited by tourists and support populations of introduced rats, both of which may act as added stressors for the scrubfowl. Using passive chew-tag and call playback surveys on five tourist-visited and five tourist-free islands, we investigated if rats and tourists negatively affect scrubfowl, and if higher rat activity …

Contribution Of Seagrass Blue Carbon Toward Carbon Neutral Policies In A Touristic And Environmentally-Friendly Island, Camila Bedulli, Paul S. Lavery, Matt Harvey, Carlos M. Duarte, Oscar Serrano

Research outputs 2014 to 2021

Estimates of organic carbon (C_org) storage by seagrass meadows which consider inter-habitat variability are essential to understand their potential to sequester carbon dioxide (CO₂) and derive robust global and regional estimates of blue carbon storage. In this study, we provide baseline estimates of seagrass extent, and soil C_org stocks and accumulation rates from different seagrass habitats at Rottnest Island (in Amphibolis spp., Posidonia spp., Halophila ovalis, and mixed Posidonia/Amphibolis spp. meadows). The C_org stocks in 0.5 m thick seagrass soil deposits, derived from 24 cores, were 5.1 ± 0.7 kg C_org m^{–2 …}

Eftilagimod Alpha, A Soluble Lymphocyte Activation Gene-3 (Lag-3) Protein Plus Pembrolizumab In Patients With Metastatic Melanoma, Victoria Atkinson, Adnan Khattak, Andrew Haydon, Melissa Eastgate, Amitesh Roy, Prashanth Prithviraj, Christian Mueller, Chrystelle Brignone, Frederic Triebel

Research outputs 2014 to 2021

© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. BACKGROUND: To evaluate the safety, tolerability, pharmacokinetics and pharmacodynamics of eftilagimod alpha (efti), a soluble lymphocyte activation gene-3 protein, in combination with the programmed cell death-1 (PD-1) antagonist pembrolizumab. METHODS: The study was divided into two parts; parts A and B, where part A was the dose escalation part and part B was an extension part of the study. Patients with metastatic melanoma were treated with efti plus the standard dose of pembrolizumab. Blood samples were assayed to determine …

The Prognostic Impact Of Circulating Tumour Dna In Melanoma Patients Treated With Systemic Therapies—Beyond Braf Mutant Detection, Gabriela Marsavela, Peter A. Johansson, Michelle R. Pereira, Ashleigh C. Mcevoy, Anna L. Reid, Cleo Robinson, Lydia Warburton, Muhammad A. Khattak, Tarek M. Meniawy, Benhur Amanuel, Michael Millward, Nicholas K. Hayward, Melanie R. Ziman, Elin S. Gray, Leslie Calapre

Research outputs 2014 to 2021

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. In this study, we evaluated the predictive value of circulating tumour DNA (ctDNA) to inform therapeutic outcomes in metastatic melanoma patients receiving systemic therapies. We analysed 142 plasma samples from metastatic melanoma patients prior to commencement of systemic therapy: 70 were treated with BRAF/MEK inhibitors and 72 with immunotherapies. Patient-specific droplet digital polymerase chain reaction assays were designed for ctDNA detection. Plasma ctDNA was detected in 56% of patients prior to first-line anti-PD1 and/or anti-CTLA-4 treatment. The detection rate in the immunotherapy cohort was comparably lower than those with BRAF inhibitors …

Seasonal Productivity Drives Aggregations Of Killer Whales And Other Cetaceans Over Submarine Canyons Of The Bremer Sub-Basin, South-Western Australia, Chandra Salgado Kent, Phil Bouchet, Rebecca Wellard, Iain Parnum, Leila Fouda, Christine Erbe

Research outputs 2014 to 2021

© 2020 Journal Compilation Cetaceans are iconic predators that serve as important indicators of marine ecosystem health. The Bremer Sub-Basin, south-western Australia, supports a diverse cetacean community including the largest documented aggregation of killer whales (Orcinus orca) in Australian waters. Knowledge of cetacean distributions is critical for managing the area's thriving ecotourism industry, yet is largely sporadic. Here we combined aerial with opportunistic ship-borne surveys during 2015-2017 to describe the occurrence of multiple cetacean species on a regional scale. We used generalised estimating equations to model variation in killer whale relative density as a function of both static and dynamic …

Morphological And Heartwood Variation Of Santalum Macgregorii In Papua New Guinea, T. Page, G. K. Jeffrey, P. Macdonell, D. Hettiarachchi, Mary C. Boyce, A. Lata, L. Oa, G. Rome

Research outputs 2014 to 2021

© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Santalum macgregorii (sandalwood), which is endemic to the southern part of Papua New Guinea (PNG), has been heavily exploited for its fragrant heartwood and is classified as threatened across its natural range. Domestication and smallholder agroforestry offer the means to preserve remaining diversity. This study was undertaken to understand the extent of remaining natural variation to support the species’s conservation and domestication. We evaluated morphological, heartwood and essential oil characters in 126 S. macgregorii trees in five populations (districts) in PNG’s Central, Gulf and Western …

Analysis Of Gluten In Dried Yeast And Yeast-Containing Products, Laura K. Allred, Mitchell G. Nye-Wood, Michelle L. Colgrave

Research outputs 2014 to 2021

Yeast are commonly used in the preparation of foods and beverages such as beer and bread and may also be used on their own as a source of nutrients and flavoring. Because of the historical connection of yeast to products made from wheat and barley, consumers maintaining a gluten-free diet can have concerns about the safety of yeast ingredients. Analyzing the safety of yeast and yeast-containing products presents some difficulties, as the yeast organisms actively degrade any gluten in the product, raising questions on the appropriateness of detection by traditional antibody-based methods. This study examines a variety of yeast and …

The Relationship Between Isometric Force-Time Characteristics And Dynamic Performance: A Systematic Review, Danny Lum, G. Gregory Haff, Tiago M. Barbosa

Research outputs 2014 to 2021

The purpose of this article was to review the data on the relationship between multi-joint isometric strength test (IsoTest) force-time characteristics (peak force, rate of force development and impulse) and dynamic performance that is available in the current literature. Four electronic databases were searched using search terms related to IsoTest. Studies were considered eligible if they were original research studies that investigated the relationships between multi-joint IsoTest and performance of dynamic movements; published in peer-reviewed journals; had participants who were athletes or active individuals who participate in recreational sports or resistance training, with no restriction on sex; and had full …

Using Climate Change Models To Inform The Recovery Of The Western Ground Parrot Pezoporus Flaviventris, Shaun W. Molloy, Allan H. Burbidge, Sarah Comer, Robert A. Davis

Research outputs 2014 to 2021

Translocation of species to areas of former habitat after threats have been mitigated is a common conservation action. However, the long-term success of reintroduction relies on identification of currently available habitat and areas that will remain, or become, habitat in the future. Commonly, a short-term view is taken, focusing on obvious and assumed threats such as predators and habitat degradation. However, in areas subject to significant climate change, challenges include correctly identifying variables that define habitat, and considering probable changes over time. This poses challenges with species such as the western ground parrot Pezoporus flaviventris, which was once relatively common …

Non-Song Vocalizations Of Humpback Whales In Western Australia, Angela Recalde-Salas, Christine Erbe, Chandra Salgado Kent, Miles Parsons

Research outputs 2014 to 2021

This study presents non-song vocalizations of humpback whales (Megaptera novaeangliae) from two migratory areas off the Western Australian coast: Geographe Bay and Port Hedland. A total of 220 sounds were identified as non-song sounds in 193 h of recordings reviewed. Of those, 68 were measured and qualitatively classified into 17 groups using their spectral features. One group (HW-02) had a high level of variation in terms of spectral slope. However, further classification using statistical classification methods was not possible because of the small sample size. Non-song sound frequencies varied from 9 Hz to 6 kHz, with the majority of sounds …

Sdg 3: Good Health And Well-Being - Framing Targets To Maximise Co-Benefits For Forests And People, Rosemary A. Mcfarlane, John Barry, Guéladio Cissé, Maya Gislason, Marta Gruca, Kerryn Higgs, Pierre Horwitz, Giang Nguyen, Jane O'Sullivan, Subhashis Sahu, Colin D. Butler

Research outputs 2014 to 2021

Key Points: The achievement of SDG 3 depends on many other SDGs; some SDGs are logically inconsistent, especially in the attempt to increase conventionally defined GDP while preserving natural capital. Any short-term gains for human health from further forest conversion (e.g. food production) creates short- and long-term, direct and indirect health risks for humans, as well as for other biota. Failure to ensure universal access to sexual and reproductive healthcare services (including family planning) will increase pressure on forests at local, regional and global scales. The burning and clearing of forests cause significant harm to health via impaired quality of …

Prokaryotic Capability To Use Organic Substrates Across The Global Tropical And Subtropical Ocean, Maria Montserrat Sala, Clara Ruiz-González, Encarna Borrull, Iñigo Azúa, Zurine Baña, Begoña Ayo, X. Antón Álvarez-Salgado, Josep M. Gasol, Carlos M. Duarte

Research outputs 2014 to 2021

Prokaryotes play a fundamental role in decomposing organic matter in the ocean, but little is known about how microbial metabolic capabilities vary at the global ocean scale and what are the drivers causing this variation. We aimed at obtaining the first global exploration of the functional capabilities of prokaryotes in the ocean, with emphasis on the under-sampled meso- and bathypelagic layers. We explored the potential utilization of 95 carbon sources with Biolog GN2 plates® in 441 prokaryotic communities sampled from surface to bathypelagic waters (down to 4,000 m) at 111 stations distributed across the tropical and subtropical Atlantic, Indian, and …

Modelling Mycorrhizal Fungi Dispersal By The Mycophagous Swamp Wallaby (Wallabia Bicolor) [Dataset], Melissa Danks, Natalie Simpson, Todd F. Elliot, C.E. Timothy Paine, Karl Vernes

Research Datasets

Despite the importance of mammal-fungal interactions, tools to estimate the mammal-assisted dispersal distances of fungi are lacking. Many mammals actively consume fungal fruiting bodies, the spores of which remain viable after passage through their digestive tract. Many of these fungi form symbiotic relationships with trees and provide an array of other key ecosystem functions. We present a flexible, general model to predict the distance a mycophagous mammal would disperse fungal spores. We modelled the probability of spore dispersal by combining animal movement data from GPS-telemetry with data on spore gut-retention time. We test this model using an exemplar generalist mycophagist, …

Human Impact Overrides Bioclimatic Drivers Of Red Fox Home Range Size Globally [Dataset], Michael Main, Robert Davis, David Blake, Harriet Mills, Tim Doherty

Research Datasets

Aim Identifying the variables that influence animal home range size is important for understanding the biological requirements of individuals and their social interactions. Given their often broad distributions, carnivores are model organisms for studying range-wide determinants of home range size. Here we test predictions about environmental determinants of home range size for one of the world’s most widely distributed carnivores, the red fox (Vulpes vulpes).

Location Global

Methods We compiled a database of 70 mean home range estimates from 62 studies and four continents, which we analysed according to site-based temperature, precipitation, environmental productivity and human influence …