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Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico
Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico
Honors Theses
The hormones gibberellin and abscisic acid are essential for plant responses to changing environmental conditions, and can send opposing signals. In wheat, the transcription factor TaABF1 plays an important role at the intersection of a gibberellin-induced/abscisic acid-suppressed pathway. When gibberellin dominates, the GA-induced gene, Amy32b, is transcribed. When abscisic acid is dominant, TaABF1 is active and it downregulates GA-induction of Amy32b, while promoting ABA-induced transcription of the gene HVA1. The activity of TaABF1 is thought to be regulated by post-translational phosphorylation at key serine residues. In this study, to determine TaABF1 phosphorylation by wheat kinases, we purified recombinant …
The Role Of Taabf1 In Abscisic Acid-Mediated Suppression Of 𝒶-Amylase Gene Expression In Cereal Grains, Lauren J. Harris
The Role Of Taabf1 In Abscisic Acid-Mediated Suppression Of 𝒶-Amylase Gene Expression In Cereal Grains, Lauren J. Harris
Honors Theses
The phytohormones gibberellin (GA) and abscisic acid (ABA) regulate important developments events in germinating seeds. Specifically, GA induces the expression of hyrolase genes, like the α-amylase gene Amy32b, which mobilizes starch reserves to be used by the embryo, and ABA suppresses this induction. Recent advancements identified ABA and GA receptors and key components in the signaling pathways, however, the mechanism of crosstalk between the hormones remains largely unknown. To further elucidate the mechanism of ABA suppression of GA-induced genes, we focused on the transcription factor TaABF1, a member of the ABA response element binding factor family. TaABF1 has been shown …