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Evaluation Of A Direct Pcr Method And The Qiagen Investigator 24plex Go! Kit For Typing Blood, Saliva And Touch Dna On Multiple Substrates, Angelita Pierre-Noel
Evaluation Of A Direct Pcr Method And The Qiagen Investigator 24plex Go! Kit For Typing Blood, Saliva And Touch Dna On Multiple Substrates, Angelita Pierre-Noel
Student Theses
In forensic DNA typing, omitting the DNA extraction procedure and adding the sample directly to the polymerase chain reaction (direct PCR) mixture has several advantages. Without extraction and purification, there is less of a risk of sample loss, sample mix up, or contamination. This study tested the feasibility of direct PCR using the Qiagen® Investigator® 24plex GO! Kit, a megaplex kit that amplifies 22 polymorphic STR markers and the Amelogenin sex determination alleles. Test samples included blood, saliva and skin cells on porous substrates, specifically denim, white cotton, polyester fabric, and paper tissue. Glass slides were used to represent non-porous …