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Chulalongkorn University

2020

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Articles 31 - 60 of 93

Full-Text Articles in Life Sciences

Spectrophotometric Estimation Of Linagliptin Using Ion-Pair Complexation And Oxidative Coupling Reactions – A Green Approach, Sunitha Gurrala, Panikumar Durga Anumolu, Sahitya Menkana, Nikitha Gandla, Keerthi Toddi Jan 2020

Spectrophotometric Estimation Of Linagliptin Using Ion-Pair Complexation And Oxidative Coupling Reactions – A Green Approach, Sunitha Gurrala, Panikumar Durga Anumolu, Sahitya Menkana, Nikitha Gandla, Keerthi Toddi

The Thai Journal of Pharmaceutical Sciences

Background: Two specific spectrophotometric methods (visible region) have been established using water as a solvent and validated for the analysis of linagliptin in API and pharmaceutical dosage forms. Materials and Methods: Method-A is established on th.


Proton Pump Inhibitors Use In Short Bowel Patients At A Thai Tertiary Hospital: A 10-Year Retrospective Observational Study, Sasisom Phadungkingtrakul, Namthip Iamkaeo, Piyathida Isutsiri, Prattana Sirisupamongkhon, Suyanee Pongthananikorn, Tippawan Siritientong Jan 2020

Proton Pump Inhibitors Use In Short Bowel Patients At A Thai Tertiary Hospital: A 10-Year Retrospective Observational Study, Sasisom Phadungkingtrakul, Namthip Iamkaeo, Piyathida Isutsiri, Prattana Sirisupamongkhon, Suyanee Pongthananikorn, Tippawan Siritientong

The Thai Journal of Pharmaceutical Sciences

Background: Proton pump inhibitors (PPIs) reduce the gastric acid secretion in short bowel patients. However, the absorptive areas of these patients are limited. In case of residual jejunum length <50 cm, the intravenous administration of PPIs should be .


Poly(Lactic-Co-Glycolic Acid) - Polyethylene Glycol Copolymer For Long-Acting Injectable: Synthesis, Characterization, And In-Vivo Study, Ravindra Kamble, Manasi Kulkarni, Swati Korake, Abhishek Pawar, Krishnat Dhekale Jan 2020

Poly(Lactic-Co-Glycolic Acid) - Polyethylene Glycol Copolymer For Long-Acting Injectable: Synthesis, Characterization, And In-Vivo Study, Ravindra Kamble, Manasi Kulkarni, Swati Korake, Abhishek Pawar, Krishnat Dhekale

The Thai Journal of Pharmaceutical Sciences

Aim: This work was aimed to develop a carrier system of poly(lactic-co-glycolic acid) (PLGA) by using polyethylene glycol (PEG) for longer action in various diseases by preparing a di-block copolymer by using Risperidone (RSP) as a drug. Methodology: PLG.


Measuring The Potential Antioxidant Activity Of Methyl Gallate: Molecular Docking Study, Sutomo S, Mohammad Rizki Fadhil Pratama Jan 2020

Measuring The Potential Antioxidant Activity Of Methyl Gallate: Molecular Docking Study, Sutomo S, Mohammad Rizki Fadhil Pratama

The Thai Journal of Pharmaceutical Sciences

Objectives: This study aims to obtain predictions of the interactions that occur between methyl gallate and various antioxidant receptors while comparing it with gallic acid as its parent compound. Materials and Methods: The protein crystal structures of.


Development And Evaluation Of Gastroretentive Formulations For Moxifloxacin Hydrochloride, Raghavendra Kumar Gunda, A. Vijayalakshmi, K. Masilamani Jan 2020

Development And Evaluation Of Gastroretentive Formulations For Moxifloxacin Hydrochloride, Raghavendra Kumar Gunda, A. Vijayalakshmi, K. Masilamani

The Thai Journal of Pharmaceutical Sciences

Purpose: The objective of the current study is to develop gastroretentive formulation for moxifloxacin HCl (hydrochloride) using various drug release modifiers and performing in vitro and in vivo evaluations. Moxifloxacin is a novel synthetic compound ha.


Assessment Of Antimicrobial Activity Of Moxifloxacin.Hcl From Gastro Retentive Formulations, Raghavendra Kumar Gunda, A. Vijayalakshmi, K. Masilamani Jan 2020

Assessment Of Antimicrobial Activity Of Moxifloxacin.Hcl From Gastro Retentive Formulations, Raghavendra Kumar Gunda, A. Vijayalakshmi, K. Masilamani

The Thai Journal of Pharmaceutical Sciences

Purpose: The objective of the present study is to assess the antimicrobial activity of gastroretentive formulation for moxifloxacin HCl and in vitro antimicrobial evaluation using the cup-plate method. Materials and Methods: Gastroretentive floating tabl.


Development And Validation Of Rp-Hplc Method For Simultaneous Determination Of Ondansetron Hydrochloride And Granisetron Hydrochloride In Their Admixtures With Pantoprazole Sodium, Al Montaser Hamed Bellah, Abdel Maaboud Ismail Mohamed, Niveen Abdel-Latif Mohamed Jan 2020

Development And Validation Of Rp-Hplc Method For Simultaneous Determination Of Ondansetron Hydrochloride And Granisetron Hydrochloride In Their Admixtures With Pantoprazole Sodium, Al Montaser Hamed Bellah, Abdel Maaboud Ismail Mohamed, Niveen Abdel-Latif Mohamed

The Thai Journal of Pharmaceutical Sciences

Objective: Development of rapid and sensitive high-performance liquid chromatography method for the quantification of ondansetron hydrochloride and granisetron hydrochloride in their admixtures with pantoprazole sodium in its dosage form using ketorolac .


Optimizing The Method For Quantification Of Apigenin And Quercetin In The Thai Herbal Sattakavata Formula By Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometer, Khuannara Narajeenrone, Jantanee Wattanarangsan, Kajee Pilakasiri, Pravit Akarasereenont, Suksalin Booranasubkajorn Jan 2020

Optimizing The Method For Quantification Of Apigenin And Quercetin In The Thai Herbal Sattakavata Formula By Ultra-Performance Liquid Chromatography Coupled With Mass Spectrometer, Khuannara Narajeenrone, Jantanee Wattanarangsan, Kajee Pilakasiri, Pravit Akarasereenont, Suksalin Booranasubkajorn

The Thai Journal of Pharmaceutical Sciences

Introduction: Sattakavata formula (SVF) is a Thai traditional medicine from Ayurved Siriraj for a relief of joint pain. It comprises six ingredients, namely, Andrographis paniculata (Burm. F.) Wall. ex Nees (AP), Cinnamomum sp. (CN), Morus alba Linn. (MA.


Anti-Tyrosinase And Anti-Melanogenic Potential Of Shallots (Allium Ascalonicum) From Various Cultivation Sites In Thailand, Thidarat Phetmanee, Thaniya Wunnakup, Thitiya Lukkunaprasit, Fameera Madaka, Sukanya Settharaksa, Narisa Kamkaen, Nirun Vipunnqeun, Laksana Charoenchai Jan 2020

Anti-Tyrosinase And Anti-Melanogenic Potential Of Shallots (Allium Ascalonicum) From Various Cultivation Sites In Thailand, Thidarat Phetmanee, Thaniya Wunnakup, Thitiya Lukkunaprasit, Fameera Madaka, Sukanya Settharaksa, Narisa Kamkaen, Nirun Vipunnqeun, Laksana Charoenchai

The Thai Journal of Pharmaceutical Sciences

Objectives: The present study aimed to investigate the anti-tyrosinase effect of shallots (Allium ascalonicum) in Thailand and to prepare an optimised shallot extract for use as a skin-lightening agent. Materials and Methods: Aqueous and ethanolic shallo.


Progress In Erectile Dysfunction Therapy Through Drug Delivery System, Mayuri Shitole, Shailesh Dugam, Rahul Tade, Neha Desai, Sopan Nangare Jan 2020

Progress In Erectile Dysfunction Therapy Through Drug Delivery System, Mayuri Shitole, Shailesh Dugam, Rahul Tade, Neha Desai, Sopan Nangare

The Thai Journal of Pharmaceutical Sciences

A man’s aptitude to acquire and continue an erection is frequently equated with masculinity and virility and can greatly influence men’s confidence. The sexual healthiness is a significant determinant of the worth of life. Erectile dysfunction (ED) a...


Ocimum Gratissimum Linn. (Lamiaceae) Protects Wistar Rats Against Inflammation And Oxidative Stress In Trinitrobenzene Sulfonic Acid-Induced Colitis, O. O. Abiodun, N. Nwadike, F. N. Ogunleye, A. S. Sosanya Jan 2020

Ocimum Gratissimum Linn. (Lamiaceae) Protects Wistar Rats Against Inflammation And Oxidative Stress In Trinitrobenzene Sulfonic Acid-Induced Colitis, O. O. Abiodun, N. Nwadike, F. N. Ogunleye, A. S. Sosanya

The Thai Journal of Pharmaceutical Sciences

Background and Aim: The activity of methanol extract of leaf of Ocimum gratissimum a plant with anti-inflammatory, antioxidant, and anti-ulcerogenic activities in a rat model of colitis was evaluated. Experimental Procedures: Colitis was induced in 30 of.


Monodora Myristica Seed Extracts Ameliorate Crude Oil Contaminated Diet-Induced Cardiotoxicity In Rats, G. T. Esiekpe, B. O. George, O. C. Orororo, A. A. Akpede, G. E. Okevwe, E. Onotugoma, J. Oguntola Jan 2020

Monodora Myristica Seed Extracts Ameliorate Crude Oil Contaminated Diet-Induced Cardiotoxicity In Rats, G. T. Esiekpe, B. O. George, O. C. Orororo, A. A. Akpede, G. E. Okevwe, E. Onotugoma, J. Oguntola

The Thai Journal of Pharmaceutical Sciences

Aim: This study investigated the ameliorative effects of Monodora myristica seed extracts in serum and heart of rats fed crude petroleum oil-polluted diet (CPOPD). ). Materials and Methods: Thirty adult Wistar rats (180 ± 5 g) were used for the study. T..


Development Of Immunofet Biosensor For Detection Of 3-Nitrotyrosine, Khine Kyi Han, Supachoke Mangmool, Korbtham Sathirakul, Kosum Chansiri, Wutthinan Jeamsaksiri, Prapimpun Wongchitrat, Montri Yasawong Jan 2020

Development Of Immunofet Biosensor For Detection Of 3-Nitrotyrosine, Khine Kyi Han, Supachoke Mangmool, Korbtham Sathirakul, Kosum Chansiri, Wutthinan Jeamsaksiri, Prapimpun Wongchitrat, Montri Yasawong

The Thai Journal of Pharmaceutical Sciences

3-Nitrotyrosine (3-NT) is one of the important biomarkers for the detection of excess of oxidative and nitrosative stress in the body, and derived from peroxynitrite-induced nitration of free and protein-bound at tyrosine residues. It was increased with .


Chemical Composition Of The Essential Oil Of Several Iris Species, Olha Mykhailenko N/A, Volodymyr Kovalyov, Tetyana Orlova Jan 2020

Chemical Composition Of The Essential Oil Of Several Iris Species, Olha Mykhailenko N/A, Volodymyr Kovalyov, Tetyana Orlova

The Thai Journal of Pharmaceutical Sciences

The essential oil was obtained by a steam distillation from the dried leaves of Iris germanica L., Iris versicolor L., Iris graminea L., and Iris halophila Pall. (Iridaceae) and was investigated by a gas chromatography-mass spectrometry (GC/MS) method. I.


Conditioned Medium Of Lipopolysaccharide-Treated Embryonic Stem Cell-Derived Mesenchymal Stem Cells Modulates In Vitro Secretion Of Inflammatory Cytokines, Faezeh Maghsood, Behrooz Johari, Nastaran Mohammadi Ghahhari, Mohammad Moradi, Mehdi Kadivar Jan 2020

Conditioned Medium Of Lipopolysaccharide-Treated Embryonic Stem Cell-Derived Mesenchymal Stem Cells Modulates In Vitro Secretion Of Inflammatory Cytokines, Faezeh Maghsood, Behrooz Johari, Nastaran Mohammadi Ghahhari, Mohammad Moradi, Mehdi Kadivar

The Thai Journal of Pharmaceutical Sciences

Background: The immunomodulatory properties of mesenchymal stem cells (MSCs) secretome have been considered by many investigators, and several preconditioning strategies such as pharmaceutical preconditioning have shown to strengthen the immunomodulatory.


Training Needs Of Thai Community Pharmacists For Primary Care Pharmacy: Qualitative Exploration, Surangkana Puengrung, Suntaree Watcharadamrongkun, Win Winit-Watjana Jan 2020

Training Needs Of Thai Community Pharmacists For Primary Care Pharmacy: Qualitative Exploration, Surangkana Puengrung, Suntaree Watcharadamrongkun, Win Winit-Watjana

The Thai Journal of Pharmaceutical Sciences

Introduction: Primary care pharmacy is crucial for community pharmacists and requires special training for some services. Objective: This study aims to explore current training needs of community pharmacists for primary care pharmacy using the Organizati.


The Effect And Cost Of Smoking Cessation Services Provided By Community Pharmacists In Thailand: A Descriptive Study Approach, Sunee Lertsinudom, Nadthatida Hansuri, Ampornpan Theeranut, Piyameth Dilokthornsakul Jan 2020

The Effect And Cost Of Smoking Cessation Services Provided By Community Pharmacists In Thailand: A Descriptive Study Approach, Sunee Lertsinudom, Nadthatida Hansuri, Ampornpan Theeranut, Piyameth Dilokthornsakul

The Thai Journal of Pharmaceutical Sciences

Introduction: The effect and cost of smoking cessation provided by pharmacists was varied and important for policy decision making. Aims: The aim of the study was to estimate the effect and cost of smoking cessation provided by community pharmacists in T.


Association Of N-Acetyltransferase 2 (Nat2) Polymorphisms And Elevated Liver Enzymes Of Myanmar Tuberculosis Patients In Thailand, Khin Sandi Thaw, Chanchira Choppradit, Pornpimol Kijsanayotin, Ratchanee Rodsiri Jan 2020

Association Of N-Acetyltransferase 2 (Nat2) Polymorphisms And Elevated Liver Enzymes Of Myanmar Tuberculosis Patients In Thailand, Khin Sandi Thaw, Chanchira Choppradit, Pornpimol Kijsanayotin, Ratchanee Rodsiri

The Thai Journal of Pharmaceutical Sciences

Isoniazid (INH) and its toxic metabolites are the major cause of anti-tuberculosis (TB) druginduced liver injury (AT-DILI). N-acetyltransferase 2 (NAT2) is involved in many metabolic pathways of INH. Previous studies demonstrated the association of NAT2 .


Protective Effect Of Croton Zambesicus Leaf Extract Against Carbon Tetrachlorideinduced Cardiac Toxicity In Rats, Rashidat Oluwafunke Ayanniyi, Hidayah Ayodeji Olumoh-Abdul, Fatimoh Idowu Ojuade, Nnaemeka Asogwa Jan 2020

Protective Effect Of Croton Zambesicus Leaf Extract Against Carbon Tetrachlorideinduced Cardiac Toxicity In Rats, Rashidat Oluwafunke Ayanniyi, Hidayah Ayodeji Olumoh-Abdul, Fatimoh Idowu Ojuade, Nnaemeka Asogwa

The Thai Journal of Pharmaceutical Sciences

Background: The leaves of Croton zambesicus (CZ) Muell Arg. (Euphorbiaceae) are used as a spice for food and in traditional medicine for the management of several disease conditions, including hypertension and diabetes. The study was carried out to deter.


Tumor Suppressive Effect Of Artemisia Annua Extract In 7, 12-Dimethylbenz[A]Anthracene-Induced Breast Cancer In Wistar Rats, Hemmat M.M. Faheem, Sameh M. M. El Nabtity, Mohamed A. M. Nassan Jan 2020

Tumor Suppressive Effect Of Artemisia Annua Extract In 7, 12-Dimethylbenz[A]Anthracene-Induced Breast Cancer In Wistar Rats, Hemmat M.M. Faheem, Sameh M. M. El Nabtity, Mohamed A. M. Nassan

The Thai Journal of Pharmaceutical Sciences

Objective: This study aimed to detect the genetic and immunohistochemical effects of the extract of Artemisia annua, a popular medicinal herb in a chemical model of breast cancer. Materials and Methods: Breast cancer was induced in female rats by 7, 12-d.


Cytotoxicity And Antiplasmodial Activity Of Alkaloid Extracts Prepared From Eight African Medicinal Plants Used In Nigeria, U. E. Uzuegbu, O. A. Opajobi, J. E. Utalor, C. O. Elu, I. Onyesom Jan 2020

Cytotoxicity And Antiplasmodial Activity Of Alkaloid Extracts Prepared From Eight African Medicinal Plants Used In Nigeria, U. E. Uzuegbu, O. A. Opajobi, J. E. Utalor, C. O. Elu, I. Onyesom

The Thai Journal of Pharmaceutical Sciences

Objectives: Malaria morbidity and mortality rates are worsening, despite the dedicated commitment to eradicate the infestation, principally because of failing chemotherapy, the mainstay of treatment, and lack of registered vaccine. Therefore, the search .


Increasing The Solubility Of Levodopa And Carbidopa Using Ionization Approach, Kitisak Krisai, Dusadee Charnvanich, Wanchai Chongcharoen Jan 2020

Increasing The Solubility Of Levodopa And Carbidopa Using Ionization Approach, Kitisak Krisai, Dusadee Charnvanich, Wanchai Chongcharoen

The Thai Journal of Pharmaceutical Sciences

The aim of this study is to increase the solubility of levodopa (LD) and carbidopa (CD) to improve loading quantity of both drugs for oral pullulan thin film preparation. Solubilization technique through ionization was employed to measure the solubility .


Cell Lysis Methods And Coenzyme Q10 Production Of Methylobacterium Strains, Nattida Mingrapoch, Somboon Tanasupawat, Dusadee Charnvanich Jan 2020

Cell Lysis Methods And Coenzyme Q10 Production Of Methylobacterium Strains, Nattida Mingrapoch, Somboon Tanasupawat, Dusadee Charnvanich

The Thai Journal of Pharmaceutical Sciences

Objective: This research aimed to investigate the effect of cell lysis methods on a quantity of coenzyme Q10 (CoQ10) produced by Methylobacterium strains. Methods: Methylobacterium organophilum NBRC 15689T and Methylobacterium strain LRY1-08 were used to.


Pharmacy Workforce In Myanmar Public Sector, Lwin Nyein Aye, Puree Anantachoti Jan 2020

Pharmacy Workforce In Myanmar Public Sector, Lwin Nyein Aye, Puree Anantachoti

The Thai Journal of Pharmaceutical Sciences

Objective: Pharmacist shortage in Myanmar was recognized, but previous local study was unavailable. This study aimed to analyze Myanmar’s pharmacist workforce situation focusing on public sector. Materials and Methods: Mixed method combining secondary ...


Inhibitory Activity Of Metabolites From Mangosteen Roots Garcinia Mangostana L. On Hepatocellular Carcinoma And Colon Cancer Cell Growth, Kedkran Koopklang Jan 2020

Inhibitory Activity Of Metabolites From Mangosteen Roots Garcinia Mangostana L. On Hepatocellular Carcinoma And Colon Cancer Cell Growth, Kedkran Koopklang

Chulalongkorn University Theses and Dissertations (Chula ETD)

Liver cancer is one of the most common deadliest diseases both worldwide and in Thailand. Because of its rich and dual blood supply, malignant tumors in the liver could grow proliferative and rapidly spread to another organ. This process was called Metastasis, the major cause of human death. Mangosteen (Garcinia mangostana L.) generally known as a queen of fruits, has been widely studied in medicinal applications for many decades, due to plenty of bioactive metabolites such as xanthones as a major component. Nonetheless, reports about bioactivities of minor components were barely known. In this study, metabolites isolated from mangosteen roots …


Biodegradation Of Polycyclic Aromatic Hydrocarbons Commonly Found In Coal Slurry Using White Rot Fungi Isolated From East Kalimantan, Retno Wulandari Jan 2020

Biodegradation Of Polycyclic Aromatic Hydrocarbons Commonly Found In Coal Slurry Using White Rot Fungi Isolated From East Kalimantan, Retno Wulandari

Chulalongkorn University Theses and Dissertations (Chula ETD)

Fifty specimens of white-rot fungi were identified and placed into 6 families and 30 species. Two new records of resupinate fungi (Basidiomycota) were discovered and reported for Indonesia: Ceriporia inflata and Ceriporia lacerata. Furthermore, a four-step screening protocol to select WRF has been conducted. These stepwise protocols have selected Trametes polyzona PBURU 12 based on its superior performance among the 30 isolates tested in this experiment with laccase as the major LMEs produced. Plackett-Burman’s design was used for screening multiple factors at a time. The result suggested that the highest positive effects influencing laccase production by PBURU 12 were glucose, …


การทดสอบความเข้ากันได้ของเอชแอลเอด้วยเทคนิคโฟลไซโตเมทรี : ทางเลือกหนึ่งของการทดสอบในผู้ป่วยขึ้นทะเบียนรอปลูกถ่ายไตจากผู้บริจาคสมองตาย, นุจนันท์ โชคทวีศักดิ์ Jan 2020

การทดสอบความเข้ากันได้ของเอชแอลเอด้วยเทคนิคโฟลไซโตเมทรี : ทางเลือกหนึ่งของการทดสอบในผู้ป่วยขึ้นทะเบียนรอปลูกถ่ายไตจากผู้บริจาคสมองตาย, นุจนันท์ โชคทวีศักดิ์

Chulalongkorn University Theses and Dissertations (Chula ETD)

การตรวจความเข้ากันได้ของ Human Leukocyte Antigen (HLA) ก่อนการปลูกถ่ายไตทำขึ้นเพื่อตรวจจับ Donor Specific Antibody (DSA) ต่อผู้บริจาค ที่อาจก่อให้เกิดปฏิกิริยาปฏิเสธอวัยวะ โดยเทคนิคที่ใช้ในปัจจุบันคือเทคนิค Complement-dependent microcytotoxicity crossmatch (CDC-XM) และ Anti-Human Globulin Complement-dependent microcytotoxicity crossmatch (AHG-CDC) มีความไวจำกัด ไม่สามารถตรวจจับ DSA ปริมาณน้อยที่ส่งผลต่ออัตราการรอดของไตได้ ขณะที่เทคนิคโฟลไซโตเมทรี (Flow cytometry crossmactch, FCXM) มีความไวสูงกว่า แต่พบปัญหาผลบวกปลอมจากแอนติบอดีที่ไม่เกี่ยวข้องทำให้ผู้ป่วยเสียโอกาสที่จะได้รับการปลูกถ่ายไต งานวิจัยนี้จึงมีวัตถุประสงค์เพื่อปรับปรุงความไวและความจำเพาะของเทคนิค FCXM โดยศึกษาในลิมโฟซัยต์จากผู้บริจาคไตสมองตายกับน้ำเหลืองตัวอย่างที่ทราบผล DSA จำนวน 20 ตัวอย่างเพื่อประเมินเทคนิคแล้วทดสอบเปรียบเทียบกับเทคนิค CDC,AHG-CDC ในกลุ่มผู้ป่วยจำนวน 199 ตัวอย่าง พบว่าในขั้นตอนการประเมินผลเทคนิค FCXM มีความไวและความจำเพาะในกลุ่ม T cell 71% และ 100% ตามลำดับ ส่วน B cell พบว่าความไวเท่ากับ 75% และความจำเพาะ 100% และสอดคล้องกับผล DSA อย่างมีนัยสำคัญทางสถิติ (p< 0.05) เมื่อทดสอบกับผู้ป่วยให้ผลสอดคล้องกับเทคนิค CDC, AHG-CDC จำนวน 190 ตัวอย่าง คิดเป็น 95.47% พบผลบวกปลอม จำนวน 8 ตัวอย่าง คิดเป็น 4.0% ผลการทดสอบความเข้ากันได้และประเมินประสิทธิภาพเทคนิค FCXM นี้สรุปได้ว่าเหมาะสมที่จะนำไปทดลองใช้ทดแทนเทคนิค CDC และ AHG-CDC แต่ควรทำการศึกษาเพิ่มเติมในกลุ่มที่มี DSA ก่อนนำไปใช้จริงในอนาคต


Genome Annotation Pipelines For Prokaryotic And Eukaryotic Microorganisms Using De Novo Short Read Genome Assembly, Songtham Anuntakarun Jan 2020

Genome Annotation Pipelines For Prokaryotic And Eukaryotic Microorganisms Using De Novo Short Read Genome Assembly, Songtham Anuntakarun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Next-generation sequencing (NGS) is the massively parallel sequencing technology that has revolutionized biological sciences. Currently, microorganism genomes have been widely studied using NGS. However, the lack of details in the draft or reference genome is a common problem in genome analysis. Therefore, this study aims to develop genome analysis pipelines for eukaryotic and prokaryotic microorganisms using public bioinformatics software and public databases. Leishmania matiniquensis and Leptospira interrogans were used as models for genome assembly and annotation in eukaryote and prokaryote, respectively. Our pipelines used SPAdes for short read assembled, AUGUSTUS and Prokka for gene prediction in eukaryotic and prokaryotic microorganisms, …


Effects Of Soluble And Insoluble Fibres On Pasting And Retrogradation Of Wheat Flour And Quality Of Bread, Arati Bhatta Jan 2020

Effects Of Soluble And Insoluble Fibres On Pasting And Retrogradation Of Wheat Flour And Quality Of Bread, Arati Bhatta

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the effect of selected fibres on pasting as well as short-term and long-term retrogradation of starch in wheat flour and to investigate the quality of fibre-added white pan breads. Two soluble fibres, tara gum (TG) and k-carrageenan (CAR), and one insoluble fibre, cellulose (CEL), were added to wheat flour at different levels (1.0, 1.5 and 2.0%). Regarding pasting behaviour, addition of soluble fibres was found to cause an increase in peak, trough, breakdown, and final viscosity. Meanwhile the addition tended to decrease setback viscosity and pasting temperature. In contrast, insoluble fibre addition posed a minimal …


Effects Of Chitosan Combined With Chitosan-Montmorillonite Nanocomposites Coating On Postharvest Quality Of 'Hom Thong' Banana Fruit, Arisa Wantat Jan 2020

Effects Of Chitosan Combined With Chitosan-Montmorillonite Nanocomposites Coating On Postharvest Quality Of 'Hom Thong' Banana Fruit, Arisa Wantat

Chulalongkorn University Theses and Dissertations (Chula ETD)

Chitosan (CTS) has been widely applied as a fruit coating in commercial fruit crops to prolong their shelf-life. In the present research, chitosan coatings were applied on ‘Hom Thong’ banana fruit, an important exported cultivar of Thailand. Postharvest qualities of ‘Hom Thong’ banana were measured after coating with 1% (w/v) low molecular weight (LMW-CTS: 65 kDa), medium molecular weight (MMW-CTS: 265 kDa), and high molecular weight (HMW-CTS: 540 kDa) chitosan for 1 min. The application of HMW-CTS showed the thickest of coated film and had effectiveness as a barrier of water vapor and O2 transmission. HMW-CTS treatment could retard fruit …