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Articles 1 - 30 of 50
Full-Text Articles in Life Sciences
Dancing Through Life: Molecular Dynamics Simulations And Network-Centric Modeling Of Allosteric Mechanisms In Hsp70 And Hsp110 Chaperone Proteins, Gabrielle Stetz, Gennady M. Verkhivker
Dancing Through Life: Molecular Dynamics Simulations And Network-Centric Modeling Of Allosteric Mechanisms In Hsp70 And Hsp110 Chaperone Proteins, Gabrielle Stetz, Gennady M. Verkhivker
Mathematics, Physics, and Computer Science Faculty Articles and Research
Hsp70 and Hsp110 chaperones play an important role in regulating cellular processes that involve protein folding and stabilization, which are essential for the integrity of signaling networks. Although many aspects of allosteric regulatory mechanisms in Hsp70 and Hsp110 chaperones have been extensively studied and significantly advanced in recent experimental studies, the atomistic picture of signal propagation and energetics of dynamics-based communication still remain unresolved. In this work, we have combined molecular dynamics simulations and protein stability analysis of the chaperone structures with the network modeling of residue interaction networks to characterize molecular determinants of allosteric mechanisms. We have shown that …
Chemical-Free Technique To Study The Ultrastructure Of Primary Cilium, Ashraf M. Mohieldin, Wissam A. Aboualaiwi, Min Gao, Surya M. Nauli
Chemical-Free Technique To Study The Ultrastructure Of Primary Cilium, Ashraf M. Mohieldin, Wissam A. Aboualaiwi, Min Gao, Surya M. Nauli
Pharmacy Faculty Articles and Research
A primary cilium is a hair-like structure with a width of approximately 200 nm. Over the past few decades, the main challenge in the study of the ultrastructure of cilia has been the high sensitivity of cilia to chemical fixation, which is required for many imaging techniques. In this report, we demonstrate a combined high-pressure freezing (HPF) and freeze-fracture transmission electron microscopy (FFTEM) technique to examine the ultrastructure of a cilium. Our objective is to develop an optimal high-resolution imaging approach that preserves cilia structures in their best natural form without alteration of cilia morphology by chemical fixation interference. Our …
Antioxidant Effect Of Spent, Ground, And Lyophilized Brew From Roasted Coffee In Frozen Cooked Pork Patties, Katrina Maryse Malixi Jully, Criselda Toto, Lilian Were
Antioxidant Effect Of Spent, Ground, And Lyophilized Brew From Roasted Coffee In Frozen Cooked Pork Patties, Katrina Maryse Malixi Jully, Criselda Toto, Lilian Were
Food Science Faculty Articles and Research
The ability of light and dark roasted coffee (1 g/kg) in varying application forms (spent ground [SCG], whole ground [WGC], or lyophilized brew [LBC]) to inhibit lipid and protein oxidation in cooked pork patties stored at −18 °C was monitored over 3 months. Malondialdehyde (MDA) for the negative control (NC) increased from 0.31 to 1.11 mg MDA/kg pork over 3 months, while pork with coffee or rosemary oleoresin had lower values at month 3 (0.054–0.40 mg MDA/kg pork). The NC had the highest values for hexanal, octanal, and nonanal (2.59, 0.10, and 0.13 mg/kg pork, respectively), while light and dark …
Real-Time Detection Of Breast Cancer Cells Using Peptidefunctionalized Microcantilever Arrays, Hashem Etayash, Keren Jiang, Sarfuddin Azmi, Thomas Thundat, Kamaljit Kaur
Real-Time Detection Of Breast Cancer Cells Using Peptidefunctionalized Microcantilever Arrays, Hashem Etayash, Keren Jiang, Sarfuddin Azmi, Thomas Thundat, Kamaljit Kaur
Pharmacy Faculty Articles and Research
Ligand-directed targeting and capturing of cancer cells is a new approach for detecting circulating tumor cells (CTCs). Ligands such as antibodies have been successfully used for capturing cancer cells and an antibody based system (CellSearch®) is currently used clinically to enumerate CTCs. Here we report the use of a peptide moiety in conjunction with a microcantilever array system to selectively detect CTCs resulting from cancer, specifically breast cancer. A sensing microcantilever, functionalized with a breast cancer specific peptide 18-4 (WxEAAYQrFL), showed significant deflection on cancer cell (MCF7 and MDA-MB-231) binding compared to when exposed to noncancerous (MCF10A and HUVEC) cells. …
Temporal Variation In Optical Properties Of Chromophoric Dissolved Organic Matter (Cdom) In Southern California Coastal Waters With Nearshore Kelp And Seagrass, Catherine D. Clark, Warren J. De Bruyn, Paige Aiona
Temporal Variation In Optical Properties Of Chromophoric Dissolved Organic Matter (Cdom) In Southern California Coastal Waters With Nearshore Kelp And Seagrass, Catherine D. Clark, Warren J. De Bruyn, Paige Aiona
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Optical properties of chromophoric dissolved organic matter (CDOM) were measured in surf zone waters in diurnal field studies at a Southern California beach with nearshore kelp and seagrass beds and intertidal plant wrack. Absorption coefficients (aCDOM(300 nm)) ranged from 0.35 m21 to 3.7 m21 with short-term variability<1 h, increases at ebb and flood tides and higher values (6 m21) during an offshore storm event. Spectral slopes (S) ranged from 0.0028 nm21 to 0.017 nm21, with higher values after the storm; S was generally inversely correlated with aCDOM(300 nm). 3-D excitation–emission matrix spectra (EEMs) for samples with lower S values had humic-type peaks associated with terrestrial material (A, C), marine microbial material (M) and protein peaks, characteristic of freshly produced organic material. Samples with high S values had no or reduced protein peaks, consistent with aged material. Fluorescent indexes (f450/f500 >2.5, BIX>1.1) were consistent with microbial aquatic sources. Leachates of senescent kelp and seagrass had protein and humic-type EEM peaks. After solar simulator irradiation (4 h), protein peaks rapidly photochemically degraded, humic-type peak C increased in intensity and peak M disappeared. Optical characteristics of kelp leachates were most similar to field samples, …
Flexibility Of Crab Chemosensory Hairs Enables Flicking Antennules To Sniff, Lindsay D. Waldrop, Matthew A. Reidenbach, M. A. R. Koehl
Flexibility Of Crab Chemosensory Hairs Enables Flicking Antennules To Sniff, Lindsay D. Waldrop, Matthew A. Reidenbach, M. A. R. Koehl
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
The first step in smelling is capture of odorant molecules from the surrounding fluid. We used lateral flagella of olfactory antennules of crabs Callinectes sapidus to study the physical process of odor capture by antennae bearing dense tufts of hair-like chemosensory sensilla (aesthetascs). Fluid flow around and through aesthetasc arrays on dynamically scaled models of lateral flagella of C. sapidus was measured by particle image velocimetry to determine how antennules sample the surrounding water when they flick. Models enabled separate evaluation of the effects of flicking speed, aesthetasc spacing, and antennule orientation. We found that crab antennules, like those of …
Elongation Factor-P At The Crossroads Of The Host-Endosymbiont Interface, Andrei Rajkovic, Anne Witzky, William Navarre, Andrew J. Darwin, Michael Ibba
Elongation Factor-P At The Crossroads Of The Host-Endosymbiont Interface, Andrei Rajkovic, Anne Witzky, William Navarre, Andrew J. Darwin, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Elongation factor P (EF-P) is an ancient bacterial translational factor that aids the ribosome in polymerizing oligo-prolines. EF-P structurally resembles tRNA and binds in-between the exit and peptidyl sites of the ribosome to accelerate the intrinsically slow reaction of peptidyl-prolyl bond formation. Recent studies have identified in separate organisms, two evolutionarily convergent EF-P post-translational modification systems (EPMS), split predominantly between gammaproteobacteria, and betaproteobacteria. In both cases EF-P receives a post-translational modification, critical for its function, on a highly conserved residue that protrudes into the peptidyl-transfer center of the ribosome. EPMSs are comprised of a gene(s) that synthesizes the precursor molecule …
Large Amplitude, Short Wave Peristalsis And Its Implications For Transport, Lindsay D. Waldrop, Laura A. Miller
Large Amplitude, Short Wave Peristalsis And Its Implications For Transport, Lindsay D. Waldrop, Laura A. Miller
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Valveless, tubular pumps are widespread in the animal kingdom, but the mechanism by which these pumps generate fluid flow is often in dispute. Where the pumping mechanism of many organs was once described as peristalsis, other mechanisms, such as dynamic suction pumping, have been suggested as possible alternative mechanisms. Peristalsis is often evaluated using criteria established in a technical definition for mechanical pumps, but this definition is based on a small-amplitude, long-wave approximation which biological pumps often violate. In this study, we use a direct numerical simulation of large-amplitude, short-wave peristalsis to investigate the relationships between fluid flow, compression frequency, …
Negative Skeletal Effects Of Locally Produced Adiponectin, Marcia J. Abbott, Theresa M. Roth, Linh Ho, Liping Wang, Dylan O'Carroll, Robert A. Nissenson
Negative Skeletal Effects Of Locally Produced Adiponectin, Marcia J. Abbott, Theresa M. Roth, Linh Ho, Liping Wang, Dylan O'Carroll, Robert A. Nissenson
Health Sciences and Kinesiology Faculty Articles
Epidemiological studies show that high circulating levels of adiponectin are associated with low bone mineral density. The effect of adiponectin on skeletal homeostasis, on osteoblasts in particular, remains controversial. We investigated this issue using mice with adipocyte-specific over-expression of adiponectin (AdTg). MicroCT and histomorphometric analysis revealed decreases (15%) in fractional bone volume in AdTg mice at the proximal tibia with no changes at the distal femur. Cortical bone thickness at mid-shafts of the tibia and at the tibiofibular junction was reduced (3–4%) in AdTg mice. Dynamic histomorphometry at the proximal tibia in AdTg mice revealed inhibition of bone formation. AdTg …
The Role Of The Pericardium In The Valveless, Tubular Heart Of The Tunicate, Ciona Savignyi, Lindsay D. Waldrop, Laura A. Miller
The Role Of The Pericardium In The Valveless, Tubular Heart Of The Tunicate, Ciona Savignyi, Lindsay D. Waldrop, Laura A. Miller
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Tunicates, small invertebrates within the phylum Chordata, possess a robust tubular heart which pumps blood through their open circulatory systems without the use of valves. This heart consists of two major components: the tubular myocardium, a flexible layer of myocardial cells that actively contracts to drive fluid down the length of the tube; and the pericardium, a stiff, outer layer of cells that surrounds the myocardium and creates a fluid-filled space between the myocardium and the pericardium. We investigated the role of the pericardium through in vivo manipulations on tunicate hearts and computational simulations of the myocardium and pericardium using …
Effect Of Sirna Pre-Exposure On Subsequent Response To Sirna Therapy, Hamidreza Montazeri Aliabadi, Parvin Mahdipoor, Cezary Kucharski, Nicole Chan, Hasan Uludag
Effect Of Sirna Pre-Exposure On Subsequent Response To Sirna Therapy, Hamidreza Montazeri Aliabadi, Parvin Mahdipoor, Cezary Kucharski, Nicole Chan, Hasan Uludag
Pharmacy Faculty Articles and Research
PURPOSE. An alternative cancer therapy based on RNA interference (RNAi) has shown considerable promise but the possibility of resistance development is not known. This study explored the possibility of therapeutic resistance against siRNA nanoparticles in human cancer cells. METHODS. Two approaches to siRNA treatment were undertaken using lipid-modified polyethylenimines, a single high concentration (shock) and repeated increasing concentrations (gradual). The targets were Mcl-1, RPS6KA5 and KSP in MDA-MB-435 cells. RESULTS. There was no evidence of resistance development in shock-treated cells, while the decrease in mRNA levels of targeted proteins was not as robust in naïve cells in gradual treatment. However, …
Cationic Cell-Penetrating Peptides Are Potent Furin Inhibitors, Bruno Ramos-Molina, Adam N. Lick, Amir Nasrolahi Shirazi, Donghoon Oh, Rakesh Tiwari, Naglaa Salem El-Sayed, Keykavous Parang, Iris Lindberg
Cationic Cell-Penetrating Peptides Are Potent Furin Inhibitors, Bruno Ramos-Molina, Adam N. Lick, Amir Nasrolahi Shirazi, Donghoon Oh, Rakesh Tiwari, Naglaa Salem El-Sayed, Keykavous Parang, Iris Lindberg
Pharmacy Faculty Articles and Research
Cationic cell-penetrating peptides have been widely used to enhance the intracellular delivery of various types of cargoes, such as drugs and proteins. These reagents are chemically similar to the multi-basic peptides that are known to be potent proprotein convertase inhibitors. Here, we report that both HIV-1 TAT47-57 peptide and the Chariot reagent are micromolar inhibitors of furin activity in vitro. In agreement, HIV-1 TAT47-57 reduced HT1080 cell migration, thought to be mediated by proprotein convertases, by 25%. In addition, cyclic polyarginine peptides containing hydrophobic moieties which have been previously used as transfection reagents also exhibited potent furin inhibition in vitro …
Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance In Pseudomonas Aeruginosa, Andrei Rajkovic, Sarah Erickson, Anne Witzky, Owen E. Branson, Jin Seo, Philip R. Gafken, Michael A. Frietas, Julian P. Whitelegge, Kym F. Faull, William Wiley Navarre, Andrew J. Darwin, Michael Ibba
Cyclic Rhamnosylated Elongation Factor P Establishes Antibiotic Resistance In Pseudomonas Aeruginosa, Andrei Rajkovic, Sarah Erickson, Anne Witzky, Owen E. Branson, Jin Seo, Philip R. Gafken, Michael A. Frietas, Julian P. Whitelegge, Kym F. Faull, William Wiley Navarre, Andrew J. Darwin, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
Elongation factor P (EF-P) is a ubiquitous bacterial protein that is required for the synthesis of poly-proline motifs during translation. In Escherichia coli and Salmonella enterica, the posttranslational β-lysylation of Lys34 by the PoxA protein is critical for EF-P activity. PoxA is absent from many bacterial species such as Pseudomonas aeruginosa, prompting a search for alternative EF-P posttranslation modification pathways. Structural analyses of P. aeruginosa EF-P revealed the attachment of a single cyclic rhamnose moiety to an Arg residue at a position equivalent to that at which β-Lys is attached to E. coli EF-P. Analysis of the genomes …
Effects Of Degree Of Roast And Application Form Of Incorporated Coffee On Inhibition Of Oxidation In Raw Refrigerated Minced Pork And Sensory Analysis Of Cooked Pork Patties With Added Coffee, Tiffany Hashimoto, Lilian Were, Criselda S. Toto, Fred Caporaso
Effects Of Degree Of Roast And Application Form Of Incorporated Coffee On Inhibition Of Oxidation In Raw Refrigerated Minced Pork And Sensory Analysis Of Cooked Pork Patties With Added Coffee, Tiffany Hashimoto, Lilian Were, Criselda S. Toto, Fred Caporaso
Student Scholar Symposium Abstracts and Posters
Roasted coffee is a source of antioxidants, but antioxidant Maillard reaction products and phenolic compound levels vary depending on degree of roast and form of coffee. The research objective was to evaluate the antioxidant effect of light and dark roasted coffee added to refrigerated minced pork (0.1 g/kg) as spent, ground, and lyophilized brewed coffee. After three weeks, all treated pork samples had TBARS values that were significantly lower than that of the negative control and comparable to rosemary. Metmyoglobin levels of meat treated with dark brew were higher than all other treatments after 3 weeks, while light brew had …
Identification Of Species In Commercially Sold Game Meats Using Dna Barcoding, Charles Quinto, Rebecca Tinoco, Rosalee S. Hellberg
Identification Of Species In Commercially Sold Game Meats Using Dna Barcoding, Charles Quinto, Rebecca Tinoco, Rosalee S. Hellberg
Student Scholar Symposium Abstracts and Posters
Game meats represent a multibillion dollar industry in the United States with high economic incentives associated with species substitution and mislabeling. However, there is currently a lack of information regarding the prevalence of mislabeled game meat on the U.S commercial market. The purpose of this study was to conduct a market survey of whole-cut game products sold within the United States to identify incidences of mislabeling using DNA barcoding. Identified species were also examined for classification as a threatened or endangered species. Fifty-four whole-cut game meat samples were collected from online distributors in the United States and sequenced across the …
Biological Degradation Of Acetaldehyde In Southern California Coastal Waters, Mary Senstad, Sovanndara Hok, Ori Barashy, Catherine D. Clark, Warren J. De Bruyn
Biological Degradation Of Acetaldehyde In Southern California Coastal Waters, Mary Senstad, Sovanndara Hok, Ori Barashy, Catherine D. Clark, Warren J. De Bruyn
Student Scholar Symposium Abstracts and Posters
Oxygenated hydrocarbons are ubiquitous in the atmosphere with levels ranging from low ppt (acetaldehyde) to low ppb (methanol). As an OH sink and an atmospheric HOx and ozone source, oxygenated hydrocarbons have a direct impact on the oxidative capacity of the atmosphere. The oceans are one of the largest sources of uncertainty in current atmospheric budget estimates of these species. A better understanding of the processes that produce and destroy these species in seawater would improve our understanding of the role of the oceans in cycling these species into or out of the atmosphere. We have measured the degradation rate …
Effects Of El Niño On Ecological Growth Along Californian And Peruvian Coasts, Alexandra Sidun
Effects Of El Niño On Ecological Growth Along Californian And Peruvian Coasts, Alexandra Sidun
Student Scholar Symposium Abstracts and Posters
This study examines the mechanisms of El Niño to further understand the ecological effects it may have along the Californian and Peruvian coasts. El Niño is the warm phase of the El Niño Southern Oscillation (ENSO). While it is a global phenomenon, these two locations were investigated for their nearly equal and opposite conditions during storm months. California, which is in an extreme state of drought, often receives heavy rainfall during El Niño and understanding its potential effects is crucial. El Niño causes upwellings along the CA coast and warm water from the region displaces the normally cool waters along …
Effect Of Phytosanitary Irradiation On The Quality Of Chandler Pummelo (Citrus Maxima (Burm.) Merr.), Akanksha Jain, J.J. Ornelas-Paz, Karina Rodriguez, Anuradha Prakash
Effect Of Phytosanitary Irradiation On The Quality Of Chandler Pummelo (Citrus Maxima (Burm.) Merr.), Akanksha Jain, J.J. Ornelas-Paz, Karina Rodriguez, Anuradha Prakash
Student Scholar Symposium Abstracts and Posters
In this study, we evaluated the chemical and physiological effect of low-dose gamma irradiation on the post-harvest quality of Chandler Pummelos (Citrus maxima (Burm.) Merr.), an emerging crop of interest in the U.S. Chandler pummelos from a local grower in California were irradiated at target doses of 150 Gy and 1000 Gy. Irradiated and untreated pummelos were stored at 12 °C for 3 weeks and at 20 °C for the 4th week to reflect three weeks of sea shipment at ideal temperature for storage of pummelos and an additional week of retail under ambient conditions. Irradiation reduced hardness of the …
Possible Precursor Events To Earthquakes And The Resulting Effects On Organic Material In The Surrounding Water Bodies, Kiyoko Nakatsui
Possible Precursor Events To Earthquakes And The Resulting Effects On Organic Material In The Surrounding Water Bodies, Kiyoko Nakatsui
Student Scholar Symposium Abstracts and Posters
Being located on the “Ring of Fire” Japan experiences many seismic events. Adding to this, it is located at the convergence of four fault lines where seismic activity will be even more frequent and severe. Although small tremors occurring in an area are indicators of an earthquake to come it only gives minutes of warning. Scientists are now trying to see if latent heat and gas release from fault lines can indicate an earthquake to come with hours or even days of warning. MODIS aqua and terra data will be analyzed in order to see if such precursors can be …
Puffs And Tufts: A Comparison Of Trichodesmium Colony Formations And Nutrient Availability Across The North Atlantic Ocean Using Remote Sensing Methods, Marc Rosenfield
Puffs And Tufts: A Comparison Of Trichodesmium Colony Formations And Nutrient Availability Across The North Atlantic Ocean Using Remote Sensing Methods, Marc Rosenfield
Student Scholar Symposium Abstracts and Posters
Trichodesmium, a genus of diazotrophic bacteria, has the capability and the population to produce a large percentage of the total oceanic N2-fixation. Though their population is known to be heavily dependent on two of the ocean’s largest limiting factors, phosphorus and iron concentrations, it is unknown what affect these factors have on the population. In this study two of the largest colony formations of Trichodesmium in the North Atlantic, tufts and puffs, are compared nutrient quality with respect to time and geographical location. Though very little nutrient in situ data was collected from the cruise, remote sensing data collected from …
Effect Of Curcumin Analog Ca27 On Androgen Receptor Translocation In Prostate Cancer Cells, Lijah Vann Gardner
Effect Of Curcumin Analog Ca27 On Androgen Receptor Translocation In Prostate Cancer Cells, Lijah Vann Gardner
Student Scholar Symposium Abstracts and Posters
The androgen receptor (AR) plays an essential role in promoting the development and progression of metastatic prostate cancer and represents an important molecular target for therapeutic intervention. We have recently described a series of synthetic analogs of the natural product diferuloylmethane (curcumin), some of which induce the down-regulation of AR expression in prostate cancer cells by an as yet largely unknown mechanism of action. While such analogs may in the long term be lead structures for the development of therapeutic drugs, we hypothesize here that they represent ideal molecular probes to identify the mechanism(s) of action for AR down-regulation. We …
Analyzation Of Metabolic Reprogramming In Drug-Resistant Mcf-7 Cells, Derick Han, Ho Leung, Andrew Vo
Analyzation Of Metabolic Reprogramming In Drug-Resistant Mcf-7 Cells, Derick Han, Ho Leung, Andrew Vo
Student Scholar Symposium Abstracts and Posters
The Warburg effect states that cancer cells mainly receive their energy from anaerobic glycolysis. Thus, mitochondria play a different role in the metabolism of cancer cells as opposed to normal, healthy cells. In chemotherapy, there is always a chance of the cancer regressing. Making drug-resistant cancer cells to analyze their metabolism may change how cancer is treated. This study aimed to create drug-resistant MCF-7 cell lines with doxorubicin in order to determine the metabolic changes that have occurred in the process of becoming resistant to drug treatments.
Effects Of Ecologically Realistic Heating Profiles On Feeding In The Intertidal Hermit Crab, Pagurus Sameulis, Paige Davis
Effects Of Ecologically Realistic Heating Profiles On Feeding In The Intertidal Hermit Crab, Pagurus Sameulis, Paige Davis
Student Scholar Symposium Abstracts and Posters
The intertidal zone is an ideal habitat to investigate effects of global warming because species living in it are very close to their physiological limits. Initial studies of invertebrate physiological responses to heat stress have employed relatively abrupt increases in temperature. My research investigates effects of ecologically more realistic temperature profiles on feeding in the intertidal hermit crab, Pagurus sameulis. Recent work in the Wright lab showed that feeding in this species is inhibited by an abrupt increase in temperature. Because temperature change in the natural environment of Pagurus is much more gradual, I hypothesize that such a gradual temperature …
Clinical Applications Of A Combination Chemotherapy Using 8-Chloro Camp And 8-Chloro Adenosine, Erik Munoz, Andrea Saich, Andrew Cox, Yu-An Peter Chang
Clinical Applications Of A Combination Chemotherapy Using 8-Chloro Camp And 8-Chloro Adenosine, Erik Munoz, Andrea Saich, Andrew Cox, Yu-An Peter Chang
Student Scholar Symposium Abstracts and Posters
Dr. Cho-Chung from the NIH first thought to use halogenated cAMP derivatives as competitive inhibitors of cAMP to slow down cancer cell mitosis. While the iodine and bromine substituted versions showed very little therapeutic actions, 8-Chloro cAMP has been shown to have strong anti-cancer effects. This has been shown in the phase II clinical trials this drug has undergone. However, these trials have had issues with solubility and toxicity. The drug is similar to vitamin C and is excreted quickly. Scientists tried to overcome this by using a peristaltic pump to give patients a continuous dosage, but this proved too …
Exploring Egr-1 As A Master Regulator Of Prostate Field Cancerization, Kristin Gabriel, Marco Bisoffi
Exploring Egr-1 As A Master Regulator Of Prostate Field Cancerization, Kristin Gabriel, Marco Bisoffi
Student Scholar Symposium Abstracts and Posters
Field cancerization denotes the presence of molecular aberrations (genetic, epigenetic, biochemical) in structurally intact cells residing in histologically normal tissues adjacent to tumors. Markers of field cancerization in prostate tissues have the potential to improve the clinical management of this malignancy through their potential to act as indicators of early disease and to serve as molecular targets for early intervention. However, for this, a detailed understanding of the functional pathways underlying field cancerization is necessary. We have recently identified four protein markers of prostate field cancerization, i.e. the key transcription factor early growth response 1 (EGR-1), the lipogenic enzyme fatty …
Evaluation Of Temperature Anomalies And Ocean Productivity During The 2004 Sumatra-Andaman Earthquake, Cora Byers, Brenna Mcnabb
Evaluation Of Temperature Anomalies And Ocean Productivity During The 2004 Sumatra-Andaman Earthquake, Cora Byers, Brenna Mcnabb
Student Scholar Symposium Abstracts and Posters
The oceanic productivity of the Indian Ocean and temperature anomalies prior to the Sumatra-Andaman earthquake (MW =9.3) and tsunami (December 26th 2004) were studied. Data was obtained via NASA’s Giovanni program to determine the effect on phytoplankton (primary producers) and temperature changes over the region of the earthquake. Seasonal trends were visible in the concentrations of chlorophyll a, coccolithophores, cyanobacteria, chlorophytes, diatoms and absorption coefficient, in addition to storm trends.
Prostate Field Cancerization – Thinking Outside The Tumor, Dor Shoshan
Prostate Field Cancerization – Thinking Outside The Tumor, Dor Shoshan
Student Scholar Symposium Abstracts and Posters
Analysis of tumor adjacent tissue is assumed to reveal a temporal record of molecular pathways that define oncogenesis. The present study determines expression of the key transcription factor and potential marker of field cancerization early growth response 1 (EGR-1) in human prostate tissues derived from prostatectomies and biopsies. Expression was detected using immunofluorescence and quantified using ImageJ software. Accordingly, EGR-1 expression was similar in cancerous and in histologically normal adjacent tissues from prostatectomy and biopsy specimens. EGR-1 could be exploited as pre-surgical disease indicator in false negative biopsies, identify areas of repeat biopsy, and add molecular information to surgical margins.
Assessing Water Quality In The Gulf Of Mexico Using Remote Sensing Data., Alliyah Thomas
Assessing Water Quality In The Gulf Of Mexico Using Remote Sensing Data., Alliyah Thomas
Student Scholar Symposium Abstracts and Posters
Hypoxia, or oxygen depletion, is a process that occurs in conjunction with eutrophication. In hypoxic conditions the dissolved oxygen levels in the water column sink to unlivable conditions for the marine organisms causing them to flee or die. Despite efforts of improvement, the annual summer Gulf of Mexico dead zone continues to be a serious threat to aquatic ecosystems. The 2012 smaller than usual dead zone was a false indicator of future improvement. The 2012 dead zone decrease was connected to the drought and inability of large amounts of runoff to flow into the gulf. Water quality analysis was done …
Effects Of Human Recreational Activity On The Tameness Of Common Loons (Gavia Immer) In Northern Wisconsin, Seth Yund
Student Scholar Symposium Abstracts and Posters
The Common Loon (Gavia immer) is an aquatic diving bird that lives in freshwater habitats in Canada and the northern U.S.. Human activity on a loon’s resident lake may affect its fitness and behavior, yet few studies identify or quantify these effects. We modified existing techniques that measure escape distances in other species to measure tameness as the distance at which individual loons dove in response to human approach by canoe. Tameness was similar between pair members, suggesting that common lake conditions or the behavior of a mate might influence the behavior. Sex, size within sex, and human activity did …
Effect Of Novel Dietary Supplement On Metabolism In Vitro And In Vivo, Robert A. Vaughan, Ailish C. White, Jason R. Beam, Nicholas P. Gannon, Randi Garcia-Smith, Roy M. Salgado, Marco Bisoffi, Kristina A. Trujillo, Carole A. Conn, Christine M. Mermier
Effect Of Novel Dietary Supplement On Metabolism In Vitro And In Vivo, Robert A. Vaughan, Ailish C. White, Jason R. Beam, Nicholas P. Gannon, Randi Garcia-Smith, Roy M. Salgado, Marco Bisoffi, Kristina A. Trujillo, Carole A. Conn, Christine M. Mermier
Health Sciences and Kinesiology Faculty Articles
Obesity is an increasingly prevalent and preventable morbidity with multiple behavioral, surgical and pharmacological interventions currently available. Commercial dietary supplements are often advertised to stimulate metabolism and cause rapid weight and/or fat loss, although few well-controlled studies have demonstrated such effects. We describe a commercially available dietary supplement (purportedly containing caffeine, catechins, and other metabolic stimulators) on resting metabolic rate in humans, and on metabolism, mitochondrial content, and related gene expression in vitro. Human males ingested either a placebo or commercially available supplement (RF) in a randomized double-blind placebo-controlled cross-over fashion. Metabolic rate, respiratory exchange ratio, and blood pressure were …