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Full-Text Articles in Life Sciences
Application Of Genome Reduction, Next Generation Sequencing, And Kaspar Genotyping In Development, Characterization, And Linkage Mapping Of Single Nucleotide Polymorphisms In The Grain Amaranths And Quinoa, Scott Matthew Smith
Theses and Dissertations
The grain amaranths (Amaranthus sp.) and quinoa (Chenopodium quinoa Willd.) are important seed crops in South America. These crops have gained international attention in recent years for their nutritional quality and tolerance to abiotic stress. We report the identification and development of functional single nucleotide polymorphism (SNP) assays for both amaranth and quinoa. SNPs were identified using a genome reduction protocol and next generation sequencing. SNP assays are based on KASPar genotyping chemistry and were detected using the Fluidigm dynamic array platform. A diversity screen consisting of 41 amaranth accessions showed that the minor allele frequency (MAF) of the amaranth …
Physiological Assessment Of Chenopodium Quinoa To Salt Stress, Arturo Jason Morales
Physiological Assessment Of Chenopodium Quinoa To Salt Stress, Arturo Jason Morales
Theses and Dissertations
The physiological responses to salt stress were measured in Chenopodium quinoa. In a greenhouse experiment, salt water was applied to the quinoa varieties, Chipaya and KU-2, and to the model halophyte Thellungiella halophila to assess their relative responses to salt stress. Height and weight data from a seven-week time course demonstrated that both cultivars exhibited greater tolerance to salt than T. halophila. In a growth chamber experiment, three quinoa cultivars, Chipaya, Ollague, and CICA 17 were hydroponically grown and physiological responses were measured with four salt treatments. Tissues collected from the growth chamber treatments were used to obtain …
Physical Mapping Of Ribosomal Genes In New World Members Of The Genus Chenopodium Using Fluorescence In Situ Hybridization, Maria C. Sederberg
Physical Mapping Of Ribosomal Genes In New World Members Of The Genus Chenopodium Using Fluorescence In Situ Hybridization, Maria C. Sederberg
Theses and Dissertations
The genus Chenopodium contains many economically important species in the New World, but is relatively understudied and poorly understood, especially in terms of evolutionary relationships. A better understanding of the structure of this genus could significantly help in breeding efforts on its cultivated members, notably the tetraploid C. quinoa and also certain varieties of C. berlandieri, also tetraploid. Of special concern is determining which diploid weed species are the most likely ancestors for C. quinoa, C. berlandieri, and the other tetraploid members of subsection Cellulata. The phylogeny can be understood in part by examining the ribosomal RNA loci and observing …
Evaluation Of Downy Mildew (Peronospora Farinosa F.Sp. Chenopodii) Resistance Among Quinoa Genotypes And Investigation Of P. Farinosa Growth Using Scanning Electron Microscopy, Leilani Kitz
Theses and Dissertations
Quinoa (Chenopodium quinoa Willd.) is a pseudocereal native to the Andean region of South America and a staple crop for subsistence farmers in the altiplano of Bolivia and Peru. Downy mildew is the most significant disease of quinoa caused by the pathogen Peronospora farinosa f.sp. chenopodii Byford. This disease greatly impacts quinoa crops with yield losses up to 99%. As fungicides are expensive for farmers, the development of resistant cultivars appears to be the most efficient means for controlling downy mildew. The quinoa germplasm bank contains high amounts of genetic diversity, some of which exhibit mildew resistance. Methods for evaluating …
Expression And Evolutionary Relationships Of The Chenopodium Quinoa 11s Seed Storage Protein Gene, Mikel Stevens, Peter J. Maughan, Daniel J. Fairbanks, Marie R. B. Balzotti, Jennifer N. Thornton, David A. Mcclellan, Eric N. Jellen, Craig E. Coleman
Expression And Evolutionary Relationships Of The Chenopodium Quinoa 11s Seed Storage Protein Gene, Mikel Stevens, Peter J. Maughan, Daniel J. Fairbanks, Marie R. B. Balzotti, Jennifer N. Thornton, David A. Mcclellan, Eric N. Jellen, Craig E. Coleman
Faculty Publications
Quinoa (Chenopodium quinoa Willd.) is a food crop cultivated by subsistence farmers and commercial growers on the high Andean plateau, primarily in Bolivia, Peru, and Chile. Present interest in quinoa is due to its tolerance of harsh environments and its nutritional value. It is thought that the seed storage proteins of quinoa, particularly the 11S globulins and 2S albumins, are responsible for the relatively high protein content and ideal amino acid balance of the quinoa seed. Here we report the genomic and cDNA sequences for two 11S genes representing two orthologous loci from the quinoa genome. Important features of the …
Identification, Sequencing, Expression And Evolutionary Relationships Of The 11s Seed Storage Protein Gene In Chenopodium Quinoa Willd., Marie Renee Barrett Balzotti
Identification, Sequencing, Expression And Evolutionary Relationships Of The 11s Seed Storage Protein Gene In Chenopodium Quinoa Willd., Marie Renee Barrett Balzotti
Theses and Dissertations
Quinoa (Chenopodium quinoa) is an Andean crop adapted to harsh environmental conditions and containing a high and well-balanced protein composition. Two seed storage proteins, the 2S albumin and 11S globulin, are the major amino acid reservoirs for the developing seedling. An in-depth study of the genes encoding these proteins is necessary to understand the source of quinoa's protein quality. Our objectives include identification and sequencing of the 11S genomic and cDNA clones, analysis of 11S expression profiles in different quinoa accessions and evaluation of evolutionary relationships between the sequence of the 11S gene in quinoa and homologous genes in other …
Simple Sequence Repeat Development, Polymorphism And Genetic Mapping In Quinoa (Chenopodium Quinoa Willd.), David Jarvis
Simple Sequence Repeat Development, Polymorphism And Genetic Mapping In Quinoa (Chenopodium Quinoa Willd.), David Jarvis
Theses and Dissertations
Quinoa is an important, highly nutritional grain crop in the Andean region of South America. DNA markers and linkage maps are important tools for the improvement of underdeveloped crops such as quinoa. The objectives of this study were to (i) develop a new set of SSR markers to augment the number of SSR markers available in quinoa, and (ii) construct a new genetic linkage map of quinoa based on SSRs using multiple recombinant-inbred line (RIL) populations. Here we report the development of 216 new polymorphic SSR markers from libraries enriched for GA, CAA, and AAT repeats, as well as 6 …
Application Of Different Levels Of Byproducts From Quinua (Chenopodium Quinoa Willd.) In The Preparation Of Rations For Guinea Pigs (Cavia Porcellus L.) In The Growing And Fattening Stages, Grover Antonio Aduviri Paredes
Application Of Different Levels Of Byproducts From Quinua (Chenopodium Quinoa Willd.) In The Preparation Of Rations For Guinea Pigs (Cavia Porcellus L.) In The Growing And Fattening Stages, Grover Antonio Aduviri Paredes
Theses and Dissertations
From quinua crop residue it is possible to obtain byproducts that can be used to feed small animals, especially guinea pigs. These byproducts can become an alternative feed to ameliorate food deficiencies generally present in the Altiplano. This study compared the effect of consumed quinua meal rations on guinea pig body weight increase and food and economic conversion. This project was developed in two phases: pre-experimental and experimental. In the first phase, genetic material appropriate for the project and the initial rations for consumption was acquired. The second phase included the experimental design where the treatments were developed. The study …
Metabolic Profile And Nitrogen Balance In Llamas (Lama Glama) Fed With Jipi Quinua And Barley Hay, Viacha Municipality - Department Of La Paz, Meliton Ajata Avircata
Metabolic Profile And Nitrogen Balance In Llamas (Lama Glama) Fed With Jipi Quinua And Barley Hay, Viacha Municipality - Department Of La Paz, Meliton Ajata Avircata
Theses and Dissertations
This research was completed in Letanías, Viacha, La Paz, Bolivia, to determine the metabolic profile in blood plasma (total protein, glucose, urea, albumin, total lipids, creatinine, triglycerides, and cholesterol concentrations), live weight gain, and Nitrogen balance in adult llamas fed with 4 rations of barley and quinoa residue. Eight adult (4-5 year old) Q’ara type llamas were used in this study. Study llamas were trained to stay in metabolic cages with feces collection harnesses. Llamas were given one of the following feed rations: ration A (20% quinoa residue, 80% barley), ration B (40% quinoa residue, 60% barley), ration C (60% …