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Development And Characterization Of Microsatellite Markers For The Grain Amaranths (Amaranthus Spp. L.), Melanie Ann Mallory
Development And Characterization Of Microsatellite Markers For The Grain Amaranths (Amaranthus Spp. L.), Melanie Ann Mallory
Theses and Dissertations
The grain amaranths (Amaranthus hypochondriacus L., A. cruentus L., and A. caudatus L.) are important pseudocereals native to the Americas that have received increased attention for their nutritional content, specifically their balance of amino acids. The objective of this project was to produce and characterize a set of highly informative, reproducible microsatellite markers for the grain amaranths. A total of 1457 clones were sequenced from three genomic libraries enriched for the microsatellite motifs AAC, AAT and AC. Of these, 353 (24%) contained unique microsatellites. An additional 29 microsatellite loci were identified among 728 BAC-end sequences of a newly developed amaranth …