Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Brigham Young University

Theses/Dissertations

Discipline
Keyword
Publication Year
Publication
File Type

Articles 31 - 60 of 1820

Full-Text Articles in Life Sciences

Potential Clean-Label Astringency Maskers For Acidic Whey Protein Beverages, Jozxelle Tongson Gao Aug 2023

Potential Clean-Label Astringency Maskers For Acidic Whey Protein Beverages, Jozxelle Tongson Gao

Theses and Dissertations

Whey Protein Ready-to-Drink (RTD) beverages are popular nutritional products due to their high protein content, complete amino acid profile, convenience, and flavor. However, astringency imparted by whey protein at low pH (pH <4.5) can significantly reduce consumer liking. Astringency is a complex problem, so it may require a multi-faceted solution. Samples were created through Response Surface Methodology (RSM), utilizing parameters determined through literature review and preliminary testing. There is evidence that sucrose may help to reduce astringency in wine. Various natural sweeteners like stevia, monk fruit extract, and sweet proteins: thaumatin and brazzein were evaluated to observe if they may reduce the astringency in acidic whey protein RTD beverages. Samples were assessed by trained quantitative descriptive analysis (QDA), consumer acceptance, and focus group panels. Formulas were instrumentally measured for pH, brix, zeta potential, and particle size. pH and brix were useful measures for monitoring processing consistency, but neither related to level of astringency of samples. Zeta potential instrumental data coincided with results of previous studies finding that a decrease in zeta potential within the range of pH 3-5 correlated with less astringency with whey protein isolates. Particle size relation to astringency is dependent on the zeta potential. A large particle size measurement does not lead to higher astringency if the molecules do not interact due to electrostatic repulsion. Analytical measurements of the samples were statistically compared for differences that may relate to less astringent beverages. The QDA panel determined the combination of 0.20% thaumatin and 0.22% stevia rebaudioside M to most reduce astringency, however this did not correlate with higher acceptability as its acceptability remained as a neither like nor dislike rating. Consumers evaluated thaumatin, brazzein, and monk fruit to be clean-label ingredients after learning about their natural sources and flavor functionalities. More research about thaumatin, monk fruit, and especially brazzein is needed to find the ideal usage levels in AWB formulation. Brazzein was least preferred according to the focus group panel, who felt it did not reduce astringency. Future research could evaluate whether a higher concentration would be more effective. These findings aim to help sensorially improve acidic whey protein beverage formulations and provide the dairy beverage industry insight on creating and marketing more clean-label products.


Exploring The Role Of Salt-Tolerant Halomonas Inoculant In Altering Plant Gene Expression To Improve Salt Tolerance In Alfalfa., Ashley Kay Miller Aug 2023

Exploring The Role Of Salt-Tolerant Halomonas Inoculant In Altering Plant Gene Expression To Improve Salt Tolerance In Alfalfa., Ashley Kay Miller

Theses and Dissertations

Soil salinity is an increasing problem facing agriculture in many parts of the world. Climate change and irrigation practices have led to decreased yields of large areas of farmland due to increased salt levels in the soil. Irrigation introduces salts to the soil that with time accumulate and threaten crop yield. In arid climates like Utah, the practice of irrigation is especially threatening to salt-sensitive crops including alfalfa (Medicago sativa). Plants that have tolerance to salt are needed to feed livestock and the world's population. One approach to address this problem is to introduce genes encoding salt tolerance into the …


An Assessment Of Cattle Traffic On, And Seed Dispersion Patterns Of, Sclerocactus Wrightiae, David D. Lariviere Aug 2023

An Assessment Of Cattle Traffic On, And Seed Dispersion Patterns Of, Sclerocactus Wrightiae, David D. Lariviere

Theses and Dissertations

Cattle grazing has been a historic use of rangelands in Utah since Pioneer settlement in the mid 1800's. Wright fishhook cactus is a small globose cactus endemic to an area of 280,000 ha in south-central Utah and was listed as endangered in October of 1979, by the U.S. Fish and Wildlife Service (USFWS). By 2010, concerns were expressed that soil compaction in proximity to the cactus posed a threat to this species, though there was no empirical data to support such concern. In order to assess the impact of cattle traffic on Wright fishhook cactus, we used an imprint device …


Evapotranspiration In Urban And Agricultural Systems With Interacting Irrigation And Nitrogen Fertilizer Treatments, Samantha R. Shumate Aug 2023

Evapotranspiration In Urban And Agricultural Systems With Interacting Irrigation And Nitrogen Fertilizer Treatments, Samantha R. Shumate

Theses and Dissertations

Nitrogen (N) fertilizer and irrigation are important inputs for all managed plant species, especially in arid and semi-arid climates. The interaction between N management and irrigation can lead to more sustainable practices. This is true for both agricultural and urban environments. Precision nutrient management has been widely adopted by producers in recent decades, and water conservation has become an important goal for turfgrass managers. In urban systems, our goal was to evaluate Kentucky bluegrass (Poa pratensis L.) to determine response to three N fertilizer rates and three irrigation rates in all possible combinations. The main and interacting treatment effects were …


Loading Force In Recreational Runners And Its Effect On Achilles Tendon Biomechanical Properties, Joshua K. Sponbeck Aug 2023

Loading Force In Recreational Runners And Its Effect On Achilles Tendon Biomechanical Properties, Joshua K. Sponbeck

Theses and Dissertations

Background: Achilles tendinopathy is a common debilitating running related injury. Achilles tendon loading force has been hypothesized as a contributor to Achilles tendinopathy. Loading force through the Achilles tendon during running is impacted by many factors such as footwear and foot strike pattern. Achilles tendon biomechanical (Achilles tendon cross-sectional area, thickness, echogenicity, stiffness, and T2*) responses to loading forces are variable and measurable in vivo. These responses impact the physiologic function of the tendon. Aims: 1) To evaluate baseline Achilles tendon biomechanical characteristics associated with a runner's habitual foot strike pattern. 2) To evaluate single running bout Achilles tendon biomechanical …


Characterization Of Phage-Host Interactions And Its Impact On Tail Fiber Evolution, Jessica Marie Lewis Aug 2023

Characterization Of Phage-Host Interactions And Its Impact On Tail Fiber Evolution, Jessica Marie Lewis

Theses and Dissertations

Beyond the scope of what our unaided eyes can see is a diverse and rich microbial community that greatly impacts each of our lives. Bacteria are microscopic organisms that can grow, develop, divide, and even rapidly evolve to adapt to new changes in their environment. Because of this, bacteria have gained the ability to thrive in extreme biological niches ranging from the high temperature and pressure surrounding hydrothermal vents to the harsh acidic conditions of our stomachs. One adaptation attracting attention, however, is their capability to survive in the presence of common and even last-resort antibiotics. The WHO recently reported …


The Effects Of Pain Levels On Static And Dynamic Postural Control And Visual Reliance In Chronic Ankle Instability Individuals, Minsub Oh Jul 2023

The Effects Of Pain Levels On Static And Dynamic Postural Control And Visual Reliance In Chronic Ankle Instability Individuals, Minsub Oh

Theses and Dissertations

Context: Chronic ankle instability (CAI) individuals experience residual symptoms including pain, swelling, ankle instability, etc. A small majority of CAI individuals report ankle pain during daily or physical activity. Despite the known negative effects of chronic pain on neuromuscular control, there is a paucity of research exploring the specific impact of chronic pain mechanisms on altered neuromuscular control in CAI individuals. Purpose: The purpose of this study was to identify the effects of pain levels on static and dynamic postural control and visual reliance in CAI individuals. Methods: A total of 60 participants were recruited, consisting of 20 CAI individuals …


The Effects Of Ketones On Brain Metabolism And Cognition, Erin Reiko Saito Jul 2023

The Effects Of Ketones On Brain Metabolism And Cognition, Erin Reiko Saito

Theses and Dissertations

The brain is one of the most energetically demanding organs within the human body and is cognitively susceptible to energetic deficits such that the rise in obesity, insulin resistance, and Alzheimer’s disease in recent decades pose a substantial threat to cognitive longevity. The therapeutic efficacy of ketones are well-established in epilepsy and are currently being applied to other disease states. Alzheimer’s disease is characterized by impairments in brain glucose uptake and metabolism in regions relevant to learning, memory, and cognition that progress with the disease. While brain glucose uptake is impaired, ketone uptake is unaltered, potentially enabling ketones to fuel …


Relationships Between Running Biomechanics And Femoral Articular Cartilage Thickness And Composition In Anterior Cruciate Ligament Reconstruction Patients, Hyunwook Lee Jul 2023

Relationships Between Running Biomechanics And Femoral Articular Cartilage Thickness And Composition In Anterior Cruciate Ligament Reconstruction Patients, Hyunwook Lee

Theses and Dissertations

Background: Patients with anterior cruciate ligament reconstruction (ACLR) have demonstrated morphological and compositional changes in femoral articular cartilage. However, it is unclear how running biomechanics are associated with femoral cartilage thickness and composition for both ACLR patients and controls. Objectives: (1) to compare measures of femoral cartilage thickness and composition between ACLR patients and matched non-ACLR controls at resting, (2) to investigate how 30 minutes of running influences the aforementioned measures for ACLR patients and controls, and (3) to investigate relationships between running biomechanics and knee cartilage thickness and composition in ACLR patients and controls. Methods: Twenty ACLR patients (age: …


Does Chronic Low Back Pain Influence Breathing Mechanics And Diaphragm Positioning? A Pilot Study, Lindsey Wensel Jun 2023

Does Chronic Low Back Pain Influence Breathing Mechanics And Diaphragm Positioning? A Pilot Study, Lindsey Wensel

Theses and Dissertations

Background and objective: Chronic low back pain (CLBP) is a complex musculoskeletal condition and often the source of the pain is not clear. A correlation has been found to exist between incidences of low back pain (LBP) and those with respiratory diseases such as COPD. This could give reason to believe that the sequence of events could be reversed, and LBP could elicit changes in respiratory function. The purpose of this study is to investigate if CLBP has an influence on breathing mechanics and the positioning of the diaphragm in the trunk. Methods: Volunteers were recruited between the ages of …


Variability Among Individual Male Runners Influences Cumulative Loading More Than Foot Strike Type, Tamarie Trotter Jun 2023

Variability Among Individual Male Runners Influences Cumulative Loading More Than Foot Strike Type, Tamarie Trotter

Theses and Dissertations

Foot strike type affects running mechanics and may influence overuse injury occurrence. Measuring the interaction between cumulative load and foot strike type may provide additional information that could eventually help with understanding injury mechanisms. The purpose of this study was to determine how foot strike type affects cumulative loading in runners, and to determine if individual metrics change throughout a 5-kilometer sub-maximal run. 30 participants ran on an instrumented treadmill for 5 km at 3.15 m/s with their preferred foot strike type (14 rearfoot strike, 16 non-rearfoot strike). Stride rate, foot strike angle, loading rate, per stride and per km …


Improving Restoration Success Of Winterfat: Influences Of Hydrophobic Seed Coatings And Planting Depth On Seedling Emergence, Kyle Andrew Cook Jun 2023

Improving Restoration Success Of Winterfat: Influences Of Hydrophobic Seed Coatings And Planting Depth On Seedling Emergence, Kyle Andrew Cook

Theses and Dissertations

In western North America, winterfat (Krascheninnikovia lanata (Pursh) A. Meeuse & Smit) is a valuable protein-rich subshrub whose restoration has been limited by poor seed flowability and low rates of seedling establishment. Seed flowability can be limited by a dense covering of hairs on winterfat fruits that can cause them to clog in mechanized equipment. Seedling establishment can be limited by premature germination of fall-sown seeds that can cause over-winter seedling mortality from freezing, pathogen attack, and winter drought. Seed coatings may provide a way to overcome both of these barriers to winterfat restoration. Coatings can compress hairs against the …


Superabsorbent Polymer Use In Rangeland Restoration, Shannon V. Nelson Apr 2023

Superabsorbent Polymer Use In Rangeland Restoration, Shannon V. Nelson

Theses and Dissertations

Soil moisture deficits often hinder rangeland revegetation efforts. Due to sheer numbers and germination timing, invasive annual species can easily outcompete those of desired seeded species for resources, including water. One way to increase seedling establishment may be to increase the amount of water available to seeded species. Superabsorbent polymers (SAPs) can absorb and release hundreds of times their weight in water and slowly release it. Concentrated SAP bands at or near seedling rooting depth may act as a soil water reservoir for germinating seedlings and increase rangeland seedling establishment. The objective of these studies was to evaluate the effect …


Assessing The Role Of Hybridization In The Evolution Of Two Common Lineages Of Lichen-Forming Fungi, Rachel Keuler Apr 2023

Assessing The Role Of Hybridization In The Evolution Of Two Common Lineages Of Lichen-Forming Fungi, Rachel Keuler

Theses and Dissertations

Once thought to be an evolutionary dead-end, hybridization is now being detected in genomes across kingdoms, perhaps even playing an integral role in evolution. In chapter 1, I investigated the potential influences of hybridization on the evolution of a group of vagrant, asexual species in the Rhizoplaca melanophthalma species group. I sequenced the mitochondrial and nuclear genomes of 55 specimens and found well-supported nuclear phylogenies of both datasets. There were, however, multiple instances of discordance between the mitochondrial and nuclear trees, which can be caused by hybridization. PhyloNet and ABBA-BABA also detected widespread hybridization among this group. In chapter 2, …


Migration Of Elk (Cervus Canadensis) And Barriers To Movement, Levi Watkins Apr 2023

Migration Of Elk (Cervus Canadensis) And Barriers To Movement, Levi Watkins

Theses and Dissertations

Movement patterns of animals are varied, complex, and can be influenced by environmental and anthropogenic factors. One form of animal movement, migration, is influenced by environmental factors that alter the timing, duration, intensity, and likelihood of migration. Additionally, features of the landscape, both natural and anthropogenic, can alter how animals move through their seasonal and home ranges. Movement patterns can be impeded or prevented by features such as railroads, rivers, and roads. Here we explore characteristics of elk (Cervus canadensis) migration by evaluating the strategies of migration exhibited and the factors that influence migration of elk in central and eastern …


Overcoming Barriers To Native Species Restoration Using Gibberellic Acid And Fungicide Seed Coatings, Amber Jo Johnson Apr 2023

Overcoming Barriers To Native Species Restoration Using Gibberellic Acid And Fungicide Seed Coatings, Amber Jo Johnson

Theses and Dissertations

Many barriers can limit restoration success. In the first chapter of this thesis, the barrier of strong seed dormancy is addressed. While dormancy benefits the species' long-term survival, it can present a challenge within a restoration scenario where rapid establishment is required. Soaking seeds in gibberellic acid (GA3) can overcome dormancy. An easier and potentially more effective method to apply this hormone is to coat seeds with a GA3-impregnated polymer, which provides a slow release of the hormone. Seed dormancy can also be mitigated by creating a favorable microsite with increased soil moisture. We compared the emergence and establishment of …


Development Of A Valid And Reliable Survey To Assess Personal, Behavioral, And Environmental Factors Surrounding Food Insecurity In A College Population, Jennette Kilgrow Apr 2023

Development Of A Valid And Reliable Survey To Assess Personal, Behavioral, And Environmental Factors Surrounding Food Insecurity In A College Population, Jennette Kilgrow

Theses and Dissertations

Objective: The aims of our present study were to develop a valid and reliable survey framed around the social cognitive theory, which would evaluate internal and external factors associated with food insecurity from the student's perspective, and to test the survey with a multi-state population. Design: The College Perspectives around Food Insecurity (CPFI) survey was developed through several revision and improvement steps using a mixed methods approach. In Phase 1 (2018-2019), data were translated into survey items and assigned to a theoretical concept (personal, behavioral, or environmental) in the social cognitive theory. In Phase 2 (2019-2020), content validity testing occurred …


Descriptions, Ecological Associations And Predictive Species Distribution Models Of New Species Of Psilochalcis Kieffer (Hymenoptera; Chalcididae) Occurring In Utah's Eastern Great Basin, Mark J. Petersen Apr 2023

Descriptions, Ecological Associations And Predictive Species Distribution Models Of New Species Of Psilochalcis Kieffer (Hymenoptera; Chalcididae) Occurring In Utah's Eastern Great Basin, Mark J. Petersen

Theses and Dissertations

The genus Psilochalcis, of the Family Chalcididae, was originally described in 1905 by Kieffer. Mainly considered an Old-World taxon, the first North American Psilochalcis were not identified until 1981 by Grissell and Schauff. Little is known about the species distributions, biologies and ecological relationships of these parasitic wasps. This dissertation describes research conducted in central Utah setting arrays of Malaise traps in 4 different habitat types common to the Great Basin at three separate locations. A result of this sampling revealed a high abundance of multiple species of Psilochalcis wasps, particularly from one location and two habitat types. Chapter 1 …


Forest Understory Plant Community Response To Prescribed Fire And Mechanical Thinning Treatments, Noelle V. Zenger Apr 2023

Forest Understory Plant Community Response To Prescribed Fire And Mechanical Thinning Treatments, Noelle V. Zenger

Theses and Dissertations

Much of the biodiversity in a forest comes from the understory vegetation. However, centuries of fire suppression in the western United States have made human-implemented disturbance necessary for reinstating natural disturbance cycles. However, the mechanical thinning and prescribed burning treatments used by land managers may differentially affect the response of the understory community. This study aimed to compare how prescribed fire and mechanical thinning treatments, with the size and severity of those treatments, affect understory plant communities in aspen-conifer forests over time. We sampled the understory community in burned, mechanically thinned, and untreated forest stands one, three, or five years …


Prevalence And Predictors Of Intern-Related Burnout In Dietetics Preceptors, Holly Hopkins Apr 2023

Prevalence And Predictors Of Intern-Related Burnout In Dietetics Preceptors, Holly Hopkins

Theses and Dissertations

Dietetics preceptors are at risk for abandoning the role of preceptor because of potential burnout. Quantifying the prevalence of burnout and identifying the predictors of burnout can help key stakeholders identify and implement strategies that prevent or mitigate burnout in dietetics preceptors. The purpose of this study was to determine the prevalence of intern-related burnout in dietetics preceptors and to identify the predictors of this burnout. This study used a cross-sectional study design. A survey instrument that included burnout and precepting measures, and items about the respondents’ personal and work characteristics was distributed to a random sample of 10,000 dietetics …


A Comparison Of Kid Goat Lipase And Microbial Lipase On The Development Of Cheddar Cheese Flavor, Reece H. Larsen Apr 2023

A Comparison Of Kid Goat Lipase And Microbial Lipase On The Development Of Cheddar Cheese Flavor, Reece H. Larsen

Theses and Dissertations

Background: Manufacturers continue to look for methods to save time in aging cheese. Complex reactions occur during cheese ripening that produce characteristic flavors and aromas. The addition of exogenous lipase enzymes is one accelerated ripening method that has been studied. Our objective was to document and compare the flavor profiles of cheddar cheeses created with either microbial lipase or animal lipase. Materials and Methods: The experiment followed a conventional formulation to create experimental batches of cheddar cheese. Kid goat lipase and three microbial lipase treatments were compared against a control. Other animal lipases were evaluated in preliminary research but were …


Dopamine D2 Receptors As A Peripheral Biomarker For Brain Dopamine Levels, Christina Small Apr 2023

Dopamine D2 Receptors As A Peripheral Biomarker For Brain Dopamine Levels, Christina Small

Theses and Dissertations

The ability to objectively index dopamine (DA) levels in the brain has the potential to revolutionize the field of neuropsychopharmacology, as having a peripheral biomarker of brain DA would enable the objective monitoring of the progression of Parkinson's disease (PD) and other DA-dependent psychiatric states. Of particular relevance to commercialization, it would provide an objective measure of treatment efficacy. We used a DA-depletion approach to determine if peripheral D2Rs are a biomarker for brain DA; mainly, the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) neurotoxin model and PD subjects, which are well-known models of DA depletion in the midbrain of rodents and humans, respectively. Dopamine …


Utilizing Isothermal Titration Calorimetry To Measure Β-Galactosidase Activity In Dairy Products, Tyler Ronald Jarrard Apr 2023

Utilizing Isothermal Titration Calorimetry To Measure Β-Galactosidase Activity In Dairy Products, Tyler Ronald Jarrard

Theses and Dissertations

The dairy industry uses enzymes to make cheese, alter product flavor, and eliminate lactose. The activities of these enzymes have been measured in clear buffered solutions, but because of the limitations of spectrophotometric methods, enzyme activities have not been measured in opaque or colored dairy products where they are used. Isothermal titration calorimetry (ITC) can be used to determine reaction kinetics in opaque and colored solutions by measuring the heat rate from enzyme-catalyzed reactions as a function of time. This study used ITC to measure β-galactosidase activity in opaque solutions of milk, sweet whey, sweet whey permeate, acid whey, and …


Halophilic Genes That Impact Plant Growth In Saline Soils, Mckay A. Meinzer Apr 2023

Halophilic Genes That Impact Plant Growth In Saline Soils, Mckay A. Meinzer

Theses and Dissertations

Many plants are highly sensitive to salt in the soil, and their growth and yield can be greatly hindered by as little as less than 1% salt concentration in the soil. Additionally, soil salinity is a growing issue globally and affects significant areas in Utah. Halophytes are plants that are adapted to grow in saline soils and have been widely studied for their physiological and molecular characteristics, but little is known about their associated microbiomes. Bacteria were isolated from the rhizosphere and as root endophytes of Salicornia rubra, Sarcocornia utahensis, and Allenrolfea occidentalis, three native Utah halophytes. Several strains of …


Real-Time Imaging And Measurement Of Compartmentalized Redox Shifts Using Novel Redox-Sensitive Biosensors: Implications In Developmental Toxicology, Brandon Mitchell Davies Apr 2023

Real-Time Imaging And Measurement Of Compartmentalized Redox Shifts Using Novel Redox-Sensitive Biosensors: Implications In Developmental Toxicology, Brandon Mitchell Davies

Theses and Dissertations

Glutathione (GSH) is a small antioxidant in the body and exists in large quantities compared to other antioxidants. The GSH redox state (Eh) helps developmental processes, however, when the GSH Eh is disrupted, cells may undergo significantly poor developmental pathways, possibly leading to long-lasting damages. Similarly, NADPH and Thioredoxin redox states can have a major impact on cellular function, viability, and response to both endogenous and exogenous toxicants. Subcellular, compartmentalized redox environments during normal or perturbed situations, specifically in the cytosol, mitochondria, and nucleus, are not well understood. Here, using the P19 neurogenesis model of cellular differentiation, the kinetics of …


Development And Validation Of A Spanish Nutrition Screening Tool For Hispanic American 3 To 5-Year-Olds, Denisse Arias Olivas Apr 2023

Development And Validation Of A Spanish Nutrition Screening Tool For Hispanic American 3 To 5-Year-Olds, Denisse Arias Olivas

Theses and Dissertations

Latinos comprise 18.9% of the population in the United States and are the largest and fastest growing minority group. Obesity prevalence was 26.2% among Hispanic children compared to 16.6% among non-Hispanic White children. The obesity epidemic among Latino children has been growing rapidly over the past three decades. Multiple barriers, such as lack of culturally appropriate screening tools, language, and lack of access to nutritional assessment and expert consultation, prevents screening of Latino children and further nutritional guidance. For this reason, prevention efforts such as the use of nutritional screening tools is required for early intervention, more so with populations …


A Comparison Of The Vascular Response To Acute Sauna Heating In Young And Middle-Aged Adults, Olivia Kathryn Leach Apr 2023

A Comparison Of The Vascular Response To Acute Sauna Heating In Young And Middle-Aged Adults, Olivia Kathryn Leach

Theses and Dissertations

BACKGROUND: Age-related declines in endothelial function have been well documented with larger declines observed in middle-aged. Passive heat exposure has been shown to be a promising method to improve vascular endothelial health, with sauna specifically being linked to reduced risk of cardiovascular disease. Increases in blood flow and shear rates associated with heat exposure are often considered to have a major influence on the observed improved endothelial function following heat exposure. The magnitude of these changes in response to sauna have not yet been defined. Therefore, the purpose of this study is to quantify and compare the vascular response to …


Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans Mar 2023

Thermal Inactivation Of Salmonella, Escherichia Coli, And Enterococcus Faecium Nrrl B-2354 In Pasta Matrices, Kristi Shannon Gowans

Theses and Dissertations

Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during mixing, extrusion, and drying. These data are essential to pasta producers seeking to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in wheat flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in raw semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. This study also assessed the appropriateness of E. faecium NRRL B-2354 as …


Developing Tools For Introducing Modifications Into The Chicken Genome, Zachary Eldon Olsen Mar 2023

Developing Tools For Introducing Modifications Into The Chicken Genome, Zachary Eldon Olsen

Theses and Dissertations

The chicken is a classic model organism that has provided key insights into embryonic development. Chicken embryos can be directly manipulated and observed during development while retaining the potential to reach adulthood. Despite this benefit, the utility of the chicken in studying development has been limited by the difficulty of introducing genetic changes to the genome. The recent development of cell culture conditions for chicken primordial germ cells (cPGC) has made it feasible to produce transgenic chickens, but there is still a lack of tools for introducing genetic modifications into cPGCs. Recombinase Mediated Cassette Exchange (RMCE) is a technique that …


The Effect Of Alcohol On Lipid Membrane-Membrane Fusion And Snare Proteins, Robert E. Coffman Jan 2023

The Effect Of Alcohol On Lipid Membrane-Membrane Fusion And Snare Proteins, Robert E. Coffman

Theses and Dissertations

Currently the treatment of alcohol use disorder is very difficult and often requires the combination of therapy and medications, with many who undertake treatment experiencing relapse over time. There is also no treatment in use to prevent the development of alcohol use disorder. It is the aim of this work to provide information that may be useful for the development of a preventative treatment for developing alcohol use disorder by elucidating more of the acute effects of alcohol use. It is known that these effects originate in the brain. Within the brain are circuits made up of neurons that communicate …