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Articles 1 - 10 of 10

Full-Text Articles in Life Sciences

Arthritis And Rosai-Dorfman Disease Of The Skin: A Diagnostic Dilemma, Irshad Nabi Soomro, Kamran Hameed Nov 1999

Arthritis And Rosai-Dorfman Disease Of The Skin: A Diagnostic Dilemma, Irshad Nabi Soomro, Kamran Hameed

Department of Pathology and Laboratory Medicine

No abstract provided.


Spinal Cord Compression Caused By Metastatic Epithelial Myoepithelial Carcinoma Of The Parotid Gland, I N. Soomro, A S. Hussainy, K Chishti, M H. Pui, S A. Khan, R Ahmed, S H. Hasan Oct 1999

Spinal Cord Compression Caused By Metastatic Epithelial Myoepithelial Carcinoma Of The Parotid Gland, I N. Soomro, A S. Hussainy, K Chishti, M H. Pui, S A. Khan, R Ahmed, S H. Hasan

Department of Pathology and Laboratory Medicine

No abstract provided.


P53 And Pcna Expression In Benign, Atypical And Malignant Meningiomas, R Ahmed, I N. Soomro, S A. Aziz, S H. Hasan Oct 1999

P53 And Pcna Expression In Benign, Atypical And Malignant Meningiomas, R Ahmed, I N. Soomro, S A. Aziz, S H. Hasan

Department of Pathology and Laboratory Medicine

Objective: Alterations: p53 genes are turning out to be the most common genetic alterations in human cancers. Due to long half-life of mutated p53, its detection is possible by immunohistochemistry. Proliferating cell nuclear antigen (PCNA) is expressed by dividing cells, hence has been shown to correlate with prognosis. We have used monoclonal antibodies protein DO-7 (p53) and PC10 (PCNA) to see whether their expression correlates with histological grading in meningethelial tumour.
Material and Methods: a Twenty nine meningiomas (20 benign, 7 atypical and 2 malignant) were selected from the records of our laboratory. p53 and PCNA expression was sought by …


Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah Jun 1999

Cholera Toxin Mediated Regulation Of The Expression Of Gq Alpha And G11 Alpha Gtp Binding Proteins, B H. Shah

Department of Biological & Biomedical Sciences

Previously it has been shown that persistent activation of the stimulatory adenylyl cyclase pathway with cholera toxin (CT) downregulates the Gs alpha polypeptide (80%) in a cAMP-independent manner in C6 glioma cells (Shah, 1997). This study was conducted to examine the short and long term effects of CT on the regulation of pertussis toxin-sensitive and -insensitive G proteins and their transcripts in C6 glioma cells. Treatment of C6 cells with CT (100 ng/ml) up to 16 h had no effect on either Gi or Gq/11 alpha proteins. However, prolonged exposure (24-48 h) caused increased expression of Gi (20-30%) and Gq/11 …


Morphological Pattern And Frequency Of Thyroid Tumors, S H. Shah, S Muzaffar, I N. Soomro, Sheema H. Hasan Jun 1999

Morphological Pattern And Frequency Of Thyroid Tumors, S H. Shah, S Muzaffar, I N. Soomro, Sheema H. Hasan

Department of Pathology and Laboratory Medicine

Objective:
The present study was done to evaluate the frequency of thyroid cancer and to find out the prevalence of histological types of thyroid tumor with respect to age and sex group.
Setting:
This study included consecutive cases of malignant tumors of thyroid gland, which were diagnosed in the Department of Pathology at the Aga Khan University Hospital, Karachi during the period of three years (1995-1997).
Methods:
These cases were evaluated on H & E stained sections from paraffin embedded 10% buffered formalin fixed tissue blocks. Special stains and immunohistochemical analysis were performed whenever required.Results: A total of 8541 malignant …


Immunohistochemical Evaluation Of Small Round Cell Tumors Of Childhood, Sajid H. Shah, Irshad N. Soomro, M. Shahid Siddiqui, Shahid Pervez, Sheema H. Hassan Apr 1999

Immunohistochemical Evaluation Of Small Round Cell Tumors Of Childhood, Sajid H. Shah, Irshad N. Soomro, M. Shahid Siddiqui, Shahid Pervez, Sheema H. Hassan

Department of Pathology and Laboratory Medicine

Objective:

This study was done to evaluate the pediatric undifferentiated small round cell tumors with immunohistochemical staining.

Setting:

The present study included consecutive cases of small round cell tumors which were diagnosed in children (<15 years) in the section of Histopathology at the Aga Khan University Hospital, Karachi during the period of two years.

Methods:

The group of undifferentiated small round cell tumors were evaluated immunohistochemically by using a panel of antibodies on sections from routinely processed, formalin fixed, paraffin embedded tissue blocks.

Results:

The category of undifferentiated small round cell tumors included rhabdomyosarcoma (23.2%), primitive neuroectodermal tumor (17.9%), non-Hodgkin’s lymphoma (16.1%), neuroblastoma (14.2%), Ewing’s sarcoma (10.7%) in order of frequency. Osteosarcoma (Small cell variant), retinoblastoma and medulloblastoma comprised …


Effect Of Amino Acids On Inhibition Of Lactate Dehydrogenase-X By Gossypol, M H. Javed, M A. Khan Mar 1999

Effect Of Amino Acids On Inhibition Of Lactate Dehydrogenase-X By Gossypol, M H. Javed, M A. Khan

Department of Biological & Biomedical Sciences

Gossypol acetic acid (GAA) has been shown to have male antifertility effects, but there are pronounced differences among animal species. In the search of endogenous effector molecules, which interfere with the functions of GAA, we have studied the in vitro effect of various amino acids on the inhibition of the purified LDH-X by GAA. Histidine, cysteine and glycine were shown to block the effect of GAA. The effects of these amino acids were concentration dependent. Histidine and glycine protection was found to be complex type in which both the Km and Vmax were decreased compared to control. Arginine, glutamic acid, …


Co-Activation Of Gi And Gq Proteins Exerts Synergistic Effect On Human Platelet Aggregation Through Activation Of Phospholipase C And Ca2+ Signalling Pathways, B H. Shah, A Siddiqui, K A. Qureshi, M Khan, S Rafi, V A. Ujan, M Y. Yakoob, Y Yaqub, H Rasheed, S A. Saeed Mar 1999

Co-Activation Of Gi And Gq Proteins Exerts Synergistic Effect On Human Platelet Aggregation Through Activation Of Phospholipase C And Ca2+ Signalling Pathways, B H. Shah, A Siddiqui, K A. Qureshi, M Khan, S Rafi, V A. Ujan, M Y. Yakoob, Y Yaqub, H Rasheed, S A. Saeed

Department of Biological & Biomedical Sciences

Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet aggregation. We show that 5-HT had no or little effect on aggregation but it did potentiate the aggregation response of epinephrine. The synergistic interaction of 5-HT (1-5 microM) and epinephrine (0.5-2 microM) was inhibited by alpha2-adrenoceptor blocker (yohimbine; IC50= 0.4 microM), calcium channel blockers (verapamil and diltiazem with IC50 of 10 and 48 mM, respectively), PLC inhibitor (U73122; IC50=6 microM) and nitric oxide (NO) donor, SNAP (IC50=1.6 …


Clinico-Morphological Pattern Of Intracranial Tumors In Children, Sajid H. Shah, Irshad N. Soomro, Akbar S. Hussainy, S H. Hassan Mar 1999

Clinico-Morphological Pattern Of Intracranial Tumors In Children, Sajid H. Shah, Irshad N. Soomro, Akbar S. Hussainy, S H. Hassan

Department of Pathology and Laboratory Medicine

Objective:
The objective of present study was to observe the histopathological pattern of intracranial tumors in children (< 15 yrs) and to correlate the site of lesion along with the histological diagnosis.
Setting:
The study included consecutive cases of intracranial tumors diagnosed in children (< 15 yrs.) in the section of histopathology at the Aga Khan University Hospital, Karachi during the period of three years.
Methods:
The initial histological evaluation of these lesions was performed on H and E stained section of paraffin embedded tissue. Special stains and immunohistochemical analysis was done whenever indicated.
Results:
During the study period, fifty-four cases of intracranial tumors were diagnosed in children. The age ranged from 1-1/2 years to 4 years with male to female ratio of 1.1:1. Astrocytoma comprised 39% of all intracranial tumors of childhood. Medulloblastoma …


Atherosclerosis And Plaque Rupture: An Update, M N. Afzal, S A. Saeed, B H. Shah Feb 1999

Atherosclerosis And Plaque Rupture: An Update, M N. Afzal, S A. Saeed, B H. Shah

Department of Biological & Biomedical Sciences

No abstract provided.