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Articles 1 - 30 of 158
Full-Text Articles in Life Sciences
Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi Reynolds, Pamela Sheaff
Charge Switch Nucleotides, John G. K. Williams, Gregory R. Bashford, Jiyan Chen, Dan Draney, Nara Narayanan, Bambi Reynolds, Pamela Sheaff
Biomedical Imaging and Biosignal Analysis Laboratory
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation Without interference from unincorporated NPs and Without illuminating the polymerase-DNA complex.
Computational Prediction Of The Agregated Structure Of Denatured Lysozyme, Pongsathorn Chotikasemsri
Computational Prediction Of The Agregated Structure Of Denatured Lysozyme, Pongsathorn Chotikasemsri
Masters Theses & Specialist Projects
Mis-folded proteins and their associated aggregates are a contributing factor in some human diseases. In this study we used the protein lysozyme as a model to define aggregation structures under denaturing conditions. Sasahara et al. (2007), Frare et al. (2009, 2006), and Rubin et al. (2008) observed conditions where heat denatured lysozyme formed fibril structures that were observed to be 8-17 nanometers in diameter under the electron microscope. Even though the crystal structure of lysozyme is known, the denatured form of this protein is still unknown. Therefore, we used Rosetta++ protein folding and blind docking software to create in silico …
Effects Of A Topical Analgesic Using Massage On Delayed Onset Muscle Soreness, Carrie E. Varvil
Effects Of A Topical Analgesic Using Massage On Delayed Onset Muscle Soreness, Carrie E. Varvil
Masters Theses & Specialist Projects
No abstract provided.
The Acute Effects Of Whole Body Vibration On Isometric Mid-Thigh Pull Performance, W. Guy Hornsby, Mark A. South, Ashley Kavanaugh, Andrew S. Layne, G. Gregory Haff, William A. Sands, Marco Cardinale, Michael W. Ramsey, Michael H. Stone
The Acute Effects Of Whole Body Vibration On Isometric Mid-Thigh Pull Performance, W. Guy Hornsby, Mark A. South, Ashley Kavanaugh, Andrew S. Layne, G. Gregory Haff, William A. Sands, Marco Cardinale, Michael W. Ramsey, Michael H. Stone
ETSU Faculty Works
Acute exposure to vibration has been suggested to produce transient increases in muscular strength (1,2,8), vertical jump displacement (4,8), and power output (2,6,7) recorded while performing various tasks. It has been hypothesized that the reported acute vibration induced increases in performance occur as a result of alterations in neuromuscular stimulation (1,3,4). Specifically, most studies have ascribed the observed improvements to the likeliness of Whole Body Vibration (WBV) in producing a “tonic vibration reflex” (TVR) in which the primary nerve endings of the Ia afferents of the muscle spindle are activated. This is thought to result in the excitation of the …
Face Gender And Emotion Expression: Are Angry Women More Like Men?, Ursula Hess, Reginald B. Adams, Karl Grammer, Robert E. Kleck
Face Gender And Emotion Expression: Are Angry Women More Like Men?, Ursula Hess, Reginald B. Adams, Karl Grammer, Robert E. Kleck
Dartmouth Scholarship
Certain features of facial appearance perceptually resemble expressive cues related to facial displays of emotion. We hypothesized that because expressive markers of anger (such as lowered eyebrows) overlap with perceptual markers of male sex, perceivers would identify androgynous angry faces as more likely to be a man than a woman (Study 1) and would be slower to classify an angry woman as a woman than an angry man as a man (Study 2). Conversely, we hypothesized that because perceptual features of fear (raised eyebrows) and happiness (a rounded smiling face) overlap with female sex markers, perceivers would be more likely …
A Functional Role For The Ventrolateral Prefrontal Cortex In Non-Spatial Auditory Cognition, Y. E. Cohen, B. E. Russ, S. J. Davis, A. E. Baker, A. L. Ackelson, R. Niteck
A Functional Role For The Ventrolateral Prefrontal Cortex In Non-Spatial Auditory Cognition, Y. E. Cohen, B. E. Russ, S. J. Davis, A. E. Baker, A. L. Ackelson, R. Niteck
Dartmouth Scholarship
Spatial and non-spatial sensory information is hypothesized to be evaluated in parallel pathways. In this study, we tested the spatial and non-spatial sensitivity of auditory neurons in the ventrolateral prefrontal cortex (vPFC), a cortical area in the non-spatial pathway. Activity was tested while non-human primates reported changes in an auditory stimulus' spatial or non-spatial features. We found that vPFC neurons were reliably modulated during a non-spatial auditory task but were not modulated during a spatial auditory task. The degree of modulation during the non-spatial task correlated positively with the monkeys' behavioral performance. These results are consistent with the hypotheses that …
Examination Of Platelet Adhesion By Streptococcus Sanguinis, Brian Mahoney
Examination Of Platelet Adhesion By Streptococcus Sanguinis, Brian Mahoney
Theses and Dissertations
Streptococcus sanguinis is a leading cause of infective endocarditis. Bacterial adhesion to platelets is likely important in the pathogenesis of infective endocarditis. Bacterial cell wall-anchored (Cwa) proteins may mediate this adhesion. To begin to test this hypothesis, S. sanguinis adhesion to platelets was examined in vitro. The requirement of 12 Cwa proteins for S. sanguinis-platelet adhesion was individually assessed, measuring adhesion of purified platelets to polystyrene wells coated with S. sanguinis strain SK36 or 12 isogenic Cwa protein mutants. Significantly fewer platelets adhered to wells coated with one mutant strain, VT1614. However, results of a whole-cell enzyme-linked immunosorbent assay (ELISA) …
The Role Of Cannabinoids And Cannabinoid Receptors In Enteric Neuronal Survival, Yan Li
The Role Of Cannabinoids And Cannabinoid Receptors In Enteric Neuronal Survival, Yan Li
Theses and Dissertations
The Endocannabinoid system has been found in the gastrointestinal tract, where it plays an important role in gut under both physiological and pathological conditions. Although the major effects of cannabinoids in the gut are mediated through effects on enteric neurons, the role of cannabinoids in the enteric nervous system is poorly understood. In the present study, we have used the primary cultures of myenteric ganglia and a newly developed fetal enteric neuronal cell line to identify whether the endocannabinoid, anandamide, affects ganglionic and neuronal survival and the pathways involved. Anandamide had a biphasic effect on ganglionic survival, increasing survival at …
Interactions Between Connected Half-Sarcomeres Produce Emergent Mechanical Behavior In A Mathematical Model Of Muscle, Kenneth S. Campbell
Interactions Between Connected Half-Sarcomeres Produce Emergent Mechanical Behavior In A Mathematical Model Of Muscle, Kenneth S. Campbell
Physiology Faculty Publications
Most reductionist theories of muscle attribute a fiber's mechanical properties to the scaled behavior of a single half-sarcomere. Mathematical models of this type can explain many of the known mechanical properties of muscle but have to incorporate a passive mechanical component that becomes approximately 300% stiffer in activating conditions to reproduce the force response elicited by stretching a fast mammalian muscle fiber. The available experimental data suggests that titin filaments, which are the mostly likely source of the passive component, become at most approximately 30% stiffer in saturating Ca2+ solutions. The work described in this manuscript used computer modeling to …
Trnas: Cellular Barcodes For Amino Acids, Ranat Banerjee, Shawn Chen, Kiley Dare, Marla Gilreath, Mette Praetorius-Ibba, Medha Raina, Noah M. Reynolds, Theresa E. Rogers, Hervé Roy, Srujana S. Yadavalli, Michael Ibba
Trnas: Cellular Barcodes For Amino Acids, Ranat Banerjee, Shawn Chen, Kiley Dare, Marla Gilreath, Mette Praetorius-Ibba, Medha Raina, Noah M. Reynolds, Theresa E. Rogers, Hervé Roy, Srujana S. Yadavalli, Michael Ibba
Biology, Chemistry, and Environmental Sciences Faculty Articles and Research
The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingly clear that the ribosome is not the only destination to which tRNAs deliver amino acids, with processes ranging from lipid modification to antibiotic biosynthesis all using aminoacyl‐tRNAs as substrates. Here we review examples of alternative functions for tRNA beyond translation, which together suggest that the role of …
Expression Of Sorl1 And A Novel Sorl1 Splice Variant In Normal And Alzheimers Disease Brain, Karrie E. Grear, I-Fang Ling, James F. Simpson, Jennifer L. Furman, Christopher R. Simmons, Shawn L. Peterson, Frederick A. Schmitt, William R. Markesbery, Qiang Liu, Julia E. Crook, Steven G. Younkin, Guojun Bu, Steven Estus
Expression Of Sorl1 And A Novel Sorl1 Splice Variant In Normal And Alzheimers Disease Brain, Karrie E. Grear, I-Fang Ling, James F. Simpson, Jennifer L. Furman, Christopher R. Simmons, Shawn L. Peterson, Frederick A. Schmitt, William R. Markesbery, Qiang Liu, Julia E. Crook, Steven G. Younkin, Guojun Bu, Steven Estus
Physiology Faculty Publications
BACKGROUND: Variations in sortilin-related receptor (SORL1) expression and function have been implicated in Alzheimers Disease (AD). Here, to gain insights into SORL1, we evaluated SORL1 expression and splicing as a function of AD and AD neuropathology, neural gene expression and a candidate single nucleotide polymorphism (SNP).
RESULTS: To identify SORL1 splice variants, we scanned each of the 46 internal SORL1 exons in human brain RNA samples and readily found SORL1 isoforms that lack exon 2 or exon 19. Quantification in a case-control series of the more abundant isoform lacking exon 2 (delta-2-SORL1), as well as the "full-length" SORL1 (FL-SORL1) isoform …
The Effect Of Male-Male Competition And Its Underlying Regulatory Mechanisms On The Electric Signal Of The Gymnotiform Fish Brachyhypopomus Gauderio, Vielka Lineth Salazar
The Effect Of Male-Male Competition And Its Underlying Regulatory Mechanisms On The Electric Signal Of The Gymnotiform Fish Brachyhypopomus Gauderio, Vielka Lineth Salazar
FIU Electronic Theses and Dissertations
Sexually-selected communication signals can be used by competing males to settle contests without incurring the costs of fighting. The ability to dynamically regulate the signal in a context-dependent manner can further minimize the costs of male aggressive interactions. Such is the case in the gymnotiform fish Brachyhypopomus gauderio, which, by coupling its electric organ discharge (EOD) waveform to endocrine systems with circadian, seasonal, and behavioral drivers, can regulate its signal to derive the greatest reproductive benefit. My dissertation research examined the functional role of the EOD plasticity observed in male B. gauderio and the physiological mechanisms that regulate the enhanced …
Body Composition Assessment Methods: A Systematic Review And Recommendations, Ygnacio Lopez Iii, Scherezade Mama, Penny Wilson, Rebecca E. Lee
Body Composition Assessment Methods: A Systematic Review And Recommendations, Ygnacio Lopez Iii, Scherezade Mama, Penny Wilson, Rebecca E. Lee
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Physical Activity Resource Attributes And Obesity In Low-Income African Americans Living In Public Housing, Kristen M. Mcalexander, Jorge A. Banda, Joshua W. Mcalexander, Rebecca E. Lee
Physical Activity Resource Attributes And Obesity In Low-Income African Americans Living In Public Housing, Kristen M. Mcalexander, Jorge A. Banda, Joshua W. Mcalexander, Rebecca E. Lee
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Depression And Percent Body Fat Are Associated With Binge Eating In A Community Sample Of African American And Hispanic Women, Penny Wilson, Paula C. Rhode, Daniel P. O'Connor, Rebecca E. Lee
Depression And Percent Body Fat Are Associated With Binge Eating In A Community Sample Of African American And Hispanic Women, Penny Wilson, Paula C. Rhode, Daniel P. O'Connor, Rebecca E. Lee
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Daily Variation In Monocyte Subsets And Toll-Like Receptor 2 And 4 Expression In Young And Old Mice, Whitney L. Breslin, Kelley Strohacker, Brian K. Mcfarlin
Daily Variation In Monocyte Subsets And Toll-Like Receptor 2 And 4 Expression In Young And Old Mice, Whitney L. Breslin, Kelley Strohacker, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Voluntary Exercise Causes Greater Weight Loss In Obese Mice, Katie C. Carpenter, Kelley Strohacker, Whitney L. Breslin, Mark R. Morrison, Joseph Potucek, Nadia Agha, Richard J. Simpson Phd, Thomas W. Lowder Phd, Brian K. Mcfarlin Phd
Voluntary Exercise Causes Greater Weight Loss In Obese Mice, Katie C. Carpenter, Kelley Strohacker, Whitney L. Breslin, Mark R. Morrison, Joseph Potucek, Nadia Agha, Richard J. Simpson Phd, Thomas W. Lowder Phd, Brian K. Mcfarlin Phd
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Regular Physical Activity Is Correlated With A Reduced Frequency Of Senescent T-Lymphocytes In Middle-Aged Men, Guillaume Spielmann, Brian K. Mcfarlin, Paula J.W. Smith, Keith Guy, Richard J. Simpson
Regular Physical Activity Is Correlated With A Reduced Frequency Of Senescent T-Lymphocytes In Middle-Aged Men, Guillaume Spielmann, Brian K. Mcfarlin, Paula J.W. Smith, Keith Guy, Richard J. Simpson
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Age-Related Differences In Monocyte Toll-Like Receptor 2/4 And Cd54 Expression In Mice, Kelley Strohacker, Whitney L. Breslin, Brian K. Mcfarlin
Age-Related Differences In Monocyte Toll-Like Receptor 2/4 And Cd54 Expression In Mice, Kelley Strohacker, Whitney L. Breslin, Brian K. Mcfarlin
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
The Muscular Benefits Of 13 Weeks Of Functional Electrical Stimulation Cycling After Spinal Cord Injury, John Ward, Stanley Fisher, Adam Thrasher
The Muscular Benefits Of 13 Weeks Of Functional Electrical Stimulation Cycling After Spinal Cord Injury, John Ward, Stanley Fisher, Adam Thrasher
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
The Relationship Between Body Image And Physical Activity In African American Women, Scherezade K. Mama, Beth E. Quill Mph, Maria E. Fernandez-Esquer Phd, Jacqueline Y. Reese-Smith, Jorge A. Banda, Rebecca E. Lee
The Relationship Between Body Image And Physical Activity In African American Women, Scherezade K. Mama, Beth E. Quill Mph, Maria E. Fernandez-Esquer Phd, Jacqueline Y. Reese-Smith, Jorge A. Banda, Rebecca E. Lee
International Journal of Exercise Science: Conference Proceedings
No abstract provided.
Sphingolipid-Induced Activation Of The Volume-Sensitive Cl− Current Is Mediated By Mitochondrial Reactive Oxygen Species, Frank Raucci
Sphingolipid-Induced Activation Of The Volume-Sensitive Cl− Current Is Mediated By Mitochondrial Reactive Oxygen Species, Frank Raucci
Theses and Dissertations
Swelling-activated Cl− current (ICl,swell) is an outwardly-rectifying current that plays an important role in cardiac electrical activity, cellular volume regulation, apoptosis, and acts as a potential effector of mechanoelectrical feedback. Persistent activation of ICl,swell has been observed in a number of models of cardiovascular disease. Previously we showed that angiotensin II (Ang II), endothelin-1 (ET-1), endothelial growth factor receptor (EGFR), and reactive oxygen species (ROS) produced by NADPH oxidase (NOX) and mitochondria are involved in the activation of ICl,swell by both osmotic swelling and Beta1 integrin stretch. Sphingolipid metabolism is modulated in several cardiopathologies and because sphingolipids are bioactive lipids …
Multiple Models Can Concurrently Explain Fatigue During Human Performance, C. Matthew Laurent†, Matt Green‡
Multiple Models Can Concurrently Explain Fatigue During Human Performance, C. Matthew Laurent†, Matt Green‡
International Journal of Exercise Science
One of the most commonly and thoroughly studied paradigms of human performance is fatigue. However, despite volumes of research there remains considerable controversy among scientists regarding definitive conclusions about the specific mechanism(s) contributing to fatigue. Within the literature there are three primary yet distinctly different governing ideas of fatigue; the traditionally referenced central model and peripheral model as well as the emerging central governor model (CGM). The CGM has recently been advocated by a limited number of researchers and is suggestive of a more integrative model of fatigue when compared the traditional peripheral and central models. However, more work is …
Determination Of Force Corresponding To Maximal Lactate Steady State In Tethered Swimming, Marcelo Papoti‡, Ricardo Vitório†, Sérgio A. Cunha†, Gustavo G. Araújo†, Adelino S.R. Silva‡, Vanessa Santhiago‡, Luiz E.B. Martins‡, Claudio A. Gobatto‡
Determination Of Force Corresponding To Maximal Lactate Steady State In Tethered Swimming, Marcelo Papoti‡, Ricardo Vitório†, Sérgio A. Cunha†, Gustavo G. Araújo†, Adelino S.R. Silva‡, Vanessa Santhiago‡, Luiz E.B. Martins‡, Claudio A. Gobatto‡
International Journal of Exercise Science
The main aim of the present investigation was to verify if the aerobic capacity (AC) measured in tethered swimming corresponds to the maximal lactate steady state (MLSS) and its correlation with 30 min and 400m free style swimming. Twenty-five swimmers were submitted to an incremental tethered swimming test (ITS) with an initial load of 20N and increments of 10N each 3min. After each stage of 3min, the athletes had 30s of interval to blood sample collections that were used to measure blood lactate concentrations ([La-]). The ACBI was determined by the abrupt increase in [La-] versus force (F). The points …
The Short-Term Effect Of Whole Body Vibration Training On Sprint Start In Collegiate Athletes, Brad Roberts*, Iain Hunter‡, Ty Hopkins‡, Brent Feland‡
The Short-Term Effect Of Whole Body Vibration Training On Sprint Start In Collegiate Athletes, Brad Roberts*, Iain Hunter‡, Ty Hopkins‡, Brent Feland‡
International Journal of Exercise Science
Whole body vibration (WBV) is characterized by a vibratory stimulus emitted throughout the body through the use of a vibrating platform on which the subject stands. Studies have shown over 30% increases in maximal explosive strength such as maximal speed biceps curl as well as increases in maximum dynamic force such as maximal sitting bench pull as the result of vibration. The purpose of this study was to investigate the effects of short term whole-body vibration on sprint starts among collegiate track athletes. On the first day eleven subjects were randomly assigned to either a non-vibration or vibration group for …
Evaluation Of Hand-To-Hand Bioelectrical Impedance Analysis For Estimating Percent Body Fat In Young Adults, Aaron M. Weaver*, Ashley C. Hill*, Joseph L. Andreacci‡, Curt B. Dixon‡
Evaluation Of Hand-To-Hand Bioelectrical Impedance Analysis For Estimating Percent Body Fat In Young Adults, Aaron M. Weaver*, Ashley C. Hill*, Joseph L. Andreacci‡, Curt B. Dixon‡
International Journal of Exercise Science
Purposes were to (a) to examine the validity and precision of a hand-to-hand bioelectrical impedance analyzer (HBIA) and (b) to determine the effect of an acute sub-maximal aerobic exercise bout on HBIA percent body fat (%BF) measures. Forty-one young adults (21 women; 20 men) visited the laboratory for body composition assessment on two separate occasions. During the control session, %BF was assessed by HBIA twice, before and immediately after 30 min of rest, and once by air-displacement plethysmography (ADP), using the BOD POD, which was considered the criterion method for comparison. During the exercise session, HBIA %BF measurements were determined …
A Kinematic And Kinetic Case Study Of A Netball Shoulder Pass, Sharon A. Hetherington*, Sibella G. King*, Denis Visentin‡, Marie-Louise Bird‡
A Kinematic And Kinetic Case Study Of A Netball Shoulder Pass, Sharon A. Hetherington*, Sibella G. King*, Denis Visentin‡, Marie-Louise Bird‡
International Journal of Exercise Science
The majority of studies analysing netball skills using force platforms have focused on reducing the risk of injury from compression and torsion forces on the knee and ankle joints during landing and pivoting. In this preliminary case study our aim was to investigate the efficacy of a combination of tools to describe the kinematic and kinetic mechanisms underlying the netball shoulder pass. The segmental movements of the netball shoulder pass were analysed from video and force platform data in order to develop a suitable methodology for use in a larger study. Peak vertical ground reaction force of 850 N was …
Psychosocial Correlates Of Physical Activity In Children And Adolescents In A Rural Community Setting, Kelly Crimi†, Larry D. Hensley‡, Kevin J. Finn‡
Psychosocial Correlates Of Physical Activity In Children And Adolescents In A Rural Community Setting, Kelly Crimi†, Larry D. Hensley‡, Kevin J. Finn‡
International Journal of Exercise Science
The purpose of this study was to evaluate the relationships between selected psychosocial factors and the physical activity behaviors of children (grade 4-8) and adolescents (grades 9-12) in a rural community setting. The Children’s Physical Activity Scale (CPAC)was used to measure the psychosocial factors of physical activity, The Physical Activity Questionnaire-Children (PAQ-C), and Physical Activity Questionnaire-Adolescents (PAQ-A) were used to measure the physical activity behaviors of the 167 participants. Results indicated that male and female physical activity behaviors were not significantly different. However, physical activity declined with age [F(8,147) = 5.44, p < 0.05, ES = 0.23]. All psychosocial factors were significantly correlated with physical activity in youth with the single highest correlation for males being “liking of exercise” (r = .61) and the highest correlation for females was “liking of games and sports”(r = .44). Stepwise regression analyses identified three subscales (liking of games and sport, liking of exercise, and parental support) in a significant prediction model of physical activity in both genders. The results indicate that children's physical activity is associated with a variety of psychosocial variables that represent import predisposing and reinforcing factors.
Balance Across Boundaries: Service Learning From An International Perspective, Teresa C. Fitts
Balance Across Boundaries: Service Learning From An International Perspective, Teresa C. Fitts
International Journal of Exercise Science
Service-Learning provides students, faculty and community partners an opportunity to go beyond classroom and laboratory instruction to the application of theoretical principles, and the development of civic responsibility by forging meaningful relationships with community partners. Service-learning also enables faculty to broaden the cultural sensitivity and international exposure of students by engaging students in directed, purposeful projects aimed at supporting the goals of both the college and the international partners. We have a unique opportunity in Exercise Science to make lasting changes and improve global conditions relating to student learning and the physical inactivity crisis.
The purpose of this article is …
Prediction Of Birth Weight By Cotinine Levels During Pregnancy In A Population Of African American Smokers, Ayman A.E. El-Mohandes, Michele Kiely, Marie G. Gantz, Susan M. Blake, M Nabil El-Khorazaty
Prediction Of Birth Weight By Cotinine Levels During Pregnancy In A Population Of African American Smokers, Ayman A.E. El-Mohandes, Michele Kiely, Marie G. Gantz, Susan M. Blake, M Nabil El-Khorazaty
Publications and Research
Objective—The goal was to investigate the association between maternal salivary cotinine levels (SCLs) and pregnancy outcome among African Americans smokers
Methods—In a randomized controlled trial conducted in 2001-2004 in Washington, D.C. 714 women (126 active smokers (18%)) were tested for SCLs at the time of recruitment and later in pregnancy. Sociodemographic health risks and pregnancy outcomes were recorded.
Results—Birth weights were significantly lower for infants born to mothers with baseline SCLs of ≥20 ng/ml compared to/ml (p=0.024), ≥50 ng/ml compared to/ml (p=0.002), ≥100 ng/ml compared to/ml (p=0.002), in bivariate analyses. In linear regression analyses adjusting for sociodemographic …