Life Sciences Commons^™

1-Nitronaphthalene Causes Ultrastructural Changes Primarily In Nonciliated Bronchiolar Epithelium Followed By Ciliated Epithelium In The Rat, John-Michael Sauer

Masters Theses

This study describes morphological changes in the lung at several time intervals following a single injection of 1-Nitronaphthalene (100 mg/kg intraperitoneal) in male Sprague-Dawley rats using transmission and scanning electron microscopy. Dilation of the smooth endoplasmic reticulum and mitochondrial distension in the nonciliated bronchiolar (Clara) cells was apparent as early as 1 hour after injection, while adjacent ciliated cell showed minimal changes. At 6 hours, when respiratory distress was apparent in the animals, Clara and ciliated cells exhibited chromatin clumping, loss of mitochondrial cristae and cytoplasmic vacuolization. Also at 6 hours, the interstitial area around the terminal bronchioles was infiltrated …

Effect Of Age On The Structure Of Meissner Corpuscles In Forepad Digital Pads Of Mice, Roger C. Mathewson

Loma Linda University Electronic Theses, Dissertations & Projects

Meissner corpuscles in forepaw digital pads of albino mice were examined by light and electron microscopy to determine structural age-changes. After 1.5 months of age, no intraepidermal nerve fibers were seen extending from corpuscles. From young (1.5-6 months) to middle age (9-15 months), corpuscles became larger and more complex, gaining more horizontally arranged terminals, associated lamellae and connective tissue. At old age (18-26 months), corpuscles became small and lobulated, appearing disorganized. There was no loosening of the corpuscle-epidermal interface. An increase in collagenous connective tissue and basal lamina duplication occurred with advancing age. Ultrastructural age-changes consisted of disorganization of the …

Serum Vitamin B-12 Levels And Urinary Methylmalonate Excretion In Total Vegetarians, Shari H. Patchett

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this research project was threefold: 1) to determine the present serum vitamin B-12 status in a select group of strict or total vegetarians all of whom had been vegetarian for six months or more. 2) to determine the daily intake of vitamin B-12, total calories, protein, % calories as fat, folic acid, and crude fiber on a total vegetarian diet. 3) to observe if subjects with low serum vitamin B-12 levels and low intakes of oral vitamin B-12 also had elevated methylmalonic acid (MMA) in the urine.

Six of the 19 serum vitamin B-12 levels were below …

Total Blood Cholesterol, Blood Triglyceride, And Blood Hdl Correlation, Rebecca Lynn Babler

Masters Theses

The purpose of this investigation was to determine the relationship between total blood cholesterol levels, blood triglyceride levels, and blood HDL levels in men and women 20 to 80 years of age. All subjects fasted for 12 hours prior to having their blood drawn.

The computer program SPSSX (SPSSX, Inc., 1988) was used along with subprograms CONDESCRIPTIVE, CORRELATION, and ANOVA. Pearson Product Moment Correlations were calculated for all subjects, for male subjects, and for female subjects.

The study revealed a low correlation coefficient between blood cholesterol levels and blood triglyceride levels for all subjects, female subjects and male subjects. There …

Molecular Genetics Of Endocrine Induced Regression Of Mouse Leydig Cell Tumors, Milton Rolando Drachenberg

Loma Linda University Electronic Theses, Dissertations & Projects

Chronic estrogen treatment results in malignant Leydig cell tumor formation in genetically susceptible strains of mice. The hypothesis that the mechanism of estrogen induced carcinogenesis includes a gene amplification event was tested using this in vivo endocrine carcinogenesis model. High molecular weight DNA isolated from liver, hyperplastic chryptorchid testes, primary Leydig cell tumors and transplanted tumor lines progressing to estrogen nondependence for growth, was studied using Southern blot analysis and the in-gel renaturation technique to identify amplification of characterized and anonymous sequences. No consistent gene amplification event could be demonstrated either in carcinogenesis or tumor progression to an estrogen nondependent …

Anticarcinogenic, Antitumor, And Antifungal Properties Of Allium Sativum (Garlic), Padma P. Tadi

Loma Linda University Electronic Theses, Dissertations & Projects

In this study the antimutagenic/anticarcinogenic, immune enhancing, antitumor and antifungal effects of a crude extract and organosulfur compounds of garlic were determined. Diallyl sulfide (DAS), and ajoene each at 100 /xg/ml, and garlic extract (GE, 12.5 mg/ml) inhibited rat liver 9000 X g supernatant (S- 9) dependent mutagenesis in Salmonella typhimurium TA100 induced by either aflatoxin B1 (AFB^ or benzo[a]pyrene (B[a]P). The garlic compounds were shown to inhibit the binding of [³H]AFB₁ to calf thymus DNA and the formation of specific AFB_1-DNA adducts. These effects on mutagenesis and DNA binding correlated with an inhibition of …

Histomorphometric Analysis Of Primate Periodontal Tissues Following Corticotomy In Orthodontics, Wuiteng Koh

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to evaluate the prevalence of specific cell types and structures at the active tooth movement site in corticotomized and non-corticotomized specimens obtained from a previous study, and to consider the hypothesis that corticotomy facilitated orthodontics is efficacious due to an inflammatory response rather than tooth-bone block movement. Specimens from 3 monkeys, each of which had a treatment and control quadrant in each arch, were sectioned, mounted on glass slides, and stained with Giemsa, haematoxylin, and Safranin stains. A histomorphometric analysis was performed with DataVoice™, a voice activated computer system which allowed for digitization of …

Aerotaxis In Halobacterium Halobium, Jemima Clara Lindbeck

Loma Linda University Electronic Theses, Dissertations & Projects

Aerotaxis, previously studied in eubacteria was investigated in the archaebacterium Halobacterium halobium. A quantitative temporal assay for aerotaxis of H. halobium was developed using computer assisted motion analysis. Aerotaxis was most pronounced in early log phase cultures. Peak aerotaxis was found to coincide with peak respiration consistent with a model in which respiration is required for aerotaxis. In a spatial assay a mutant strain lacking the proton motive force (pmf)-generating pigments, bacteriorhodopsin (bR) and halorhodopsin (hR), exhibited enhanced aerotaxis bands relative to the bR⁺hR⁺ parent. The enhanced aerotaxis in the mutant cells was not due solely to …

A Flow Cytometric Analysis Of Immunocyte Populations In Peripheral Blood And Pleural Effusions Of Patients With Cancer, Mandy L. Bohn

Loma Linda University Electronic Theses, Dissertations & Projects

Two-color flow cytometric analysis was performed on paired samples of peripheral blood (PB) and pleural effusions (PE) of patients with metastatic malignancies. The purpose of these analyses was to test the hypothesis that there are significant differences in the distribution of immunocyte populations in the PE compared to that of the PB.

The majority of the pleural fluid immunocytes were CD3+ cells. The ratio of CD4+ to CD8+ cells was higher in the PE compared to PB. Levels of CD8+, CD19+ and CD14+ cells were not significantly different in the PE compared to the levels in the PB. CD16+CD56+ cells …

The Role Of T Cells In The Pathogenesis Of Periapical Lesions, John B. Wallstrom

Loma Linda University Electronic Theses, Dissertations & Projects

The pulps of mandibular molars of 15 athymic and 15 conventional rats were surgically exposed and left open to their oral flora. Each group was divided into 3 subgroups of 5 animals each. The rats were sacrificed after their pulps were exposed for 2, 4, or 8 weeks. After fixing, decalcifying, and embedding, the specimens were sectioned and stained with H and E. They were then examined under a microscopic grid and quantified by percentages of surface areas of bone, connective tissue, bone marrow, intrabony spaces, periapical lesions, and numbers of osteoclasts. This was done using a DataVoice computerized data …

Primary Productivity And Species Composition Of A Reconstructed Wetland In Eastern Kentucky, Eric C. Webb

Morehead State Theses and Dissertations

A thesis presented to the faculty of the Department of Biological and Environmental Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Eric C. Webb on May 19, 1991.

The Feasibility Of Foamed Ink Use In Flexographic Printing, Anthony Malerich

Honors Theses

Flexographic and rotogravure printing are two of the three predominant printing processes; the other is offset lithography. They are very similar in that they each transfer a very fluid, low viscosity ink from an ink fountain to an engraved cylinder. Foamed ink is a completely water based liquid ink that is foamed by the introduction of air at press side, and presents numerous advantages over conventional organic solvent based and water based inks. The prime advantage is that foamed inks are a completely aqueous, environmentally safe system. Another benefit is the cost savings associated with foamed ink. Ink consumption is …

Nutrient Chemistry Of A Reconstructed Forested Wetland In Rowan County, Kentucky, David M. Blevins

Morehead State Theses and Dissertations

A thesis presented to the faculty of the Department of Biological and Environmental Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by David M. Blevins on May 10, 1991.

An Analysis Of The Ph Tolerance And Substrate Preference Of Isolated Skeletal Muscle Mitochondria From Bufo Marinus And Rana Catesbeiana, Jeffrey Mark Duerr

Dissertations and Theses

The concept of maximal oxygen consumption (VO₂ max) implies that there is a limit to the amount of oxygen that can be consumed by an organism in a given time. Many attempts have been made to delineate exactly what factor or factors are limiting to VO₂ max. Current models focus on the steps over which oxygen must traverse in order to get from the ambient air to the mitochondria. However, no model has addressed the issue of whether or not carbon dioxide flux may impose a limit to VO₂ max. Impedance of carbon dioxide flux may have …

Senegalese Women: A Comparative Analysis Of Economic Development In Sine-Saloum And Dakar, Sarah-Joy Hunter

College Honors Program

The complex nexus of Dependency theories, Self-Reliance, and Afro-centric feminisms frame this exploration into economic development strategies Senegalese women deploy in the peanut and fishing sectors to ensure the survival of their families and communities.

Molecular Genetic Analysis Of The Interaction Between The Bacterial Pathogen Xanthomonas Campestris Pv. Vesicatoria And The Tomato Plant, Peter M. Antall

Senior Scholar Papers

X. campestris pv. vesieatoria (Xcv) Is a bacterial pathogen of tomato and pepper. Xcv is able to infect most tomato lines. causing bacterial spot disease. which can lead to death. A strain of Xcv exists. however. which elicits a resistance response In a particular tomato line. ThIs avirulent strain carries a single locus. the aviru1ence gene avrRxv, whIch specifies the resistance inducing activity. Accordlng to Flor's gene for gene model, a corresponding gene must exist in the resistant plant. Evidence exists for this gene but it has not yet been cloned. In this study, the role ofthe avrRxv gene product …

Running With Running Shoes Versus Barefoot Running: A Videographical Analysis, Nikolaos Stergiou

Student Work

The purpose of this study was to kinematically describe the movements of the lower extremities in running with running shoes and running barefoot. Another purpose of this study was to identify and compare the anatomical and mechanical adaptations that occur. Seven college age females, members of the University of Nebraska at Omaha cross-country team, and one local competitive long-distance female runner 27 yrs old, were subjects of this study. All subjects were free of any physical disability that could have caused an impaired performance.

All subjects completed one testing session consisting of three acceptable trials each with and without their …

The Demographic And Morphological Characteristics Of Black Bears In The Smoky Mountains, Peter Kleppinger Mclean

Doctoral Dissertations

Data on age, sex, body measurements, survival, and reproductive condition were collected from 1702 black bears (Ursus americanus) trapped in the Smoky Mountains (SM), 1972-1989. The age structure suggested a lightly to moderately exploited bear population. Bears of Great Smoky Mountain National Park (GSMNP or Park) were significantly (P = 0.026) older (mean = 4.52 yr) than those of Pisgah (3.86 yr) National Forest (P < 0.076). SM females had a mean minimum reproductive age of 4.2 yr, birth interval of 2.4 yr, and litter size of 2.0 cubs. The percentage of lactating females was significantly associated with age (X² = 20.6, 2 df, P < 0.001), and lactation rates were significantly related to white oak mast production (r² = 0.51, P < 0.01). The annual mortality rate was 26% and was lowest for Park bears (22%) and highest for those of the national forests (30%). Density ranged from 0.09 to 0.35 bears/km², and the intrinsic rate of growth (2-11%) indicated a slightly to moderately increasing population.

Both extrinsic and intrinsic factors govern the population. Food …

Use Of Antisense Rna In The Study Of Essential Cell Division Genes In Escherichia Coli, Quynh V. Nguyen

Honors Theses

No abstract provided.

Investigating The Viability Of Yeast Expressing Histone From Extrachromosomal Dna : Progress In Construction Of A Plasmid Bearing One Copy Of Each Yeast Histone Gene, Ralph Donaldson Hellams Jr.

Master's Theses

Much of the current information on histones has been based on in vitro studies. The original goal of this research was to construct a mutant strain of Saccharomyces cerevisiae containing all chromosomal copies of the yeast histone genes made nonfunctional. This project would demonstrate whether such a strain could be rescued by a plasmid carrying the wild-type copies of the four core histone genes. Furthermore, this yeast strain construct would allow future investigations to take advantage of histone mutant analysis in vivo. The critical step in beginning this work was the construction of a plasmid which contained the genes …

The Effect Of Wet Pressing On Filler Stratification, Patrick T. Gish

Paper Engineering Senior Theses

Research was conducted to determine the effect of press loading upon filler stratification with the intention of observing the development of a stratified sheet.

Press conditions were held constant with the exception of pressure applied to the sheet. The sheet was then split and examined for filler content. The paper tested was highly refined, very moist, and of a heavy basis weight.

Results indicated a gradual trend of increased stratification with increased press loading. Filler moved toward the direction that water was leaving the sheet.

Hazard Perception And Preparation By Cross-Country Skiers In Utah, Kevin J. Kobe

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This thesis examined how cross-country skiers perceive and prepare for winter hazards. A self-completion questionnaire was administered to cross-country skiers in northern Utah. The questions on the questionnaire were designed to explore the relationships that affect how skiers perceive and prepare for winter hazards. Additionally, the situation where skiers put themselves at risk due to lack of information as opposed to skiers placing themselves at risk through the desire to confront nature's dangers was explored. Variables that were contained in these relationships were correlated and the degree of correlation was measured.

Those that sought information on the day surveyed were …

Photosynthetic Capacity, Leaf Size And Plant Height In Wheat (Triticum Aestivum L.), Deborah L. Bishop

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Plant breeders often examine leaf size, plant height and photosynthetic capacity in an effort to increase wheat yield. This study was concerned with the relationship between these parameters in dwarf and semidwarf wheat cultivars (Triticum aestivum L.) with a wide range in flag leaf size. Photosynthetic capacity was measured at anthesis using photosynthesis versus intercellular CO2 response curves to determine maximum photosynthetic rate and ribulose-1,5- bisphosphate carboxylase efficiency. Leaf area, chlorophyll concentration, stomatal density, interveinal distance and dry mass partitioning were also examined. Smaller flag leaves had greater carboxylation efficiency and closer vein spacing. Dwarf wheat had higher chlorophyll …

Changes In The Vulvular And Vestibular Tissue Of The Bovine During The Estrous Cycle As Determined By The Use Of Near Infrared Interactance, Robert Alan Kunzler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Near infrared reflectance spectroscopy is routinely used for the analysis of quality components of feedstuffs. Near infrared spectrophotometers, coupled with a fiber optic probe, could enable direct measurements of the live animal. This study was conducted to characterize changes in the vulva and vestibule during the bovine estrous cycle using near infrared (NI) spectroscopy. Sixteen cycling Holstein cows were observed for estrus twice daily from 40 days postpartum for three estrous cycles or until conception was verified. In addition, weekly rectal palpations, cowside milk progesterone tests, and tailhead chalk were used to aid in estrous detection. Near infrared spectra of …

Development And Evaluation Of An Expert System For Use As An Aid In Culling Dairy Cattle, Max L. Checketts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An expert system for identifying cows to be culled, MAXCULL, was programmed to run on an IBM or compatible personal computer. It was designed to be used with Dairy Herd Improvement (DHI) records as an aid in decision making. MAXCULL used fifty-two health, seventeen reproduction, and thirty-nine production rules in the analysis.

MAXCULL was initially developed using two expert system tools. VP-Expert and Super Expert both had inductive abilities and were reasonably priced. VP-Expert was selected to continue the development of the MAXCULL system. The program used a rule-based method of storing knowledge, which was obtained from literature reviewed in …

Evaluation Of Antiviral Compounds Against Avian Influenza, Evan W. Call

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tests in vitro for antiviral activity against avian influenza viruses, A/Turkey/Sanpete/85 (H6N8) and A/Turkey/Sanpete/86 (H10N9), isolated in Sanpete County, Utah, utilized known antiviral agents, amantadine⋅HCl (adamantanamine hydrochloride) and ribavirin (1-β-D ribofuranosyl-1,2,4-triazole-3-carboxamide). The testing involved evaluation of seven drug concentrations. Maximum tolerated dose, minimum inhibitory concentration and therapeutic indexes were determined for each drug used. Both drugs demonstrated reasonable antiviral activity. Then they were tested against the H10N9 strain in 10-day-old Broad White turkey poults. Large- and small-particle aerosol delivery systems were the delivery methods utilized. Various parameters, including water and feed consumption, health score (based on visible signs), recoverable lung …

Hydrogen Uptake Genes And Nitrogen Fixation Efficiency Of Rhizobium Species In Symbiosis With Alfalfa, Chickpea And Pigeonpea, G. Mustafa Sajid

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The plasmids pDN211 and pDNll, isolated from the gene bank of the Rhizobium japonicum strain I-110, have been reported to complement two different Nif⁺ Hup^· (nitrogen fixation positive and hydrogen uptake negative) mutants. A 5.9-kb Hindiii DNA fragment of the cosmid pHU52, isolated from the gene bank of R. japonicum strain 122DES, has been reported to code for the two polypeptide subunits of uptake hydrogenase. To determine homology between the structural genes of uptake hydrogenase of the two strains, a Southern blot of the Hindiii restriction fragments of the plasmids pDN211 and pDN11 was hybridized to the 5.9-kb …

Potassium And Sodium Interrelations In Alfalfa Phenotypes Grown On Calcareous Soil, Suresh S. Dhumal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three greenhouse experiments were conducted with three phenotypes of alfalfa (Medicago sativa L.) obtained from a potassium (K)-deficient field and with their diallel crosses grown on low K soil. The first experiment was conducted to study the partitioning and broad-sense heritability of K and sodium (Na) between leaves and stems of the three phenotypes which were deficient in K and exhibited normal (N), marginal chlorotic (M), and white spot chlorotic (W) leaflets. The second experiment was conducted to study the partitioning of K and Na in leaves, stems, and roots as influenced by 32 alfalfa crosses obtained from diallel …

The Effects Of Policy Development And Organizational Structure On The Performance Of Range Livestock Development Projects In Africa, Gregory Karl Perrier

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Range professionals are frequently involved in the arrangement of range development projects, both domestically and internationally. The success record of such projects has been uneven, particularly for projects in developing nations. The objective of this study was to provide range specialists a better understanding of potential management obstacles and barriers to success. This is accomplished through an examination of the management of three AID-funded pastoral development projects in Africa: the Tanzanian Masai Livestock and Range Management Project, the Somali Central Rangelands Development Project, and the Lesotho Lard Conservation and Range Development Project.

The study examined the effects of two important …

Reproductive Strategies Of Northern Goshawks And Cooper's Hawks In North-Central New Mexico, Patricia L. Kennedy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I assumed that the reproductive strategies of the two Accipiter species investigated in this study, the northern Goshawk (A. gentilis) and the Cooper's hawk (A. cooperii), would be similar to the strategies of other monogamous raptors with sex role partitioning. Any differences in the species' reproductive strategies, particularly hunting strategies, were expected to be a function of their size differences. Contrary to predictions, results indicated that, in north-central New Mexico during brood rearing, both species had similar hunting strategies and utilized similar prey species. These results also indicated that the reproductive strategies of the Cooper's hawk …