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The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü
The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü
Turkish Journal of Botany
Watermelon fruit is very sensitive to ethylene, showing rapid deterioration and water-soaking upon ethylene exposure. The objective of this study was to isolate and determine the expressions of ethylene receptor genes in watermelon fruit. As a result of PCR amplification of cDNAs obtained via RT-PCR of total RNA from watermelon fruit with degenerate primers, 3 partial cDNAs were obtained. After extension of the 3' and 5' ends of the cDNAs via 3' and 5' RACE analysis, 3 full-length genes designated as CL-ETR1, CL-ERS1, and CL-ETR2 were obtained. Upon cloning and sequencing, it was determined that the CL-ETR1, CL-ERS1, and CL-ETR2 …