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Isolation And Expression Analysis Of Ethylene Receptor Genes From Melon(Cucumis Melo) Fruit, Yaşar Karakurt, Nazan Gökteki̇n, Hali̇me Ünlü
Isolation And Expression Analysis Of Ethylene Receptor Genes From Melon(Cucumis Melo) Fruit, Yaşar Karakurt, Nazan Gökteki̇n, Hali̇me Ünlü
Turkish Journal of Botany
Ethylene receptors are involved in ethylene sensing and signal transduction. This study was conducted to isolate genes encoding ethylene receptors from melon fruit and to determine their expression during postharvest fruit ripening. The amplification of melon fruit cDNA with degenerate primers revealed two partial cDNAs. Upon 3' and 5' RACE analysis of the cDNAs, two full length genes designated Cm-ETR1 and Cm-ETR2 were obtained. Cloning and sequencing of the genes showed that Cm-ETR1 and Cm-ETR2 genes were 2320 and 2537 bp in length, respectively. Cm-ETR1 had a predicted open reading frame (ORF) of 2223 nucleotides encoding a protein of 740 …
The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü
The Molecular Characterization And Expression Analyses Of Ethylene Receptor Genes From Watermelon Fruit, Yaşar Karakurt, Muhammet Tonguç, Hali̇me Ünlü
Turkish Journal of Botany
Watermelon fruit is very sensitive to ethylene, showing rapid deterioration and water-soaking upon ethylene exposure. The objective of this study was to isolate and determine the expressions of ethylene receptor genes in watermelon fruit. As a result of PCR amplification of cDNAs obtained via RT-PCR of total RNA from watermelon fruit with degenerate primers, 3 partial cDNAs were obtained. After extension of the 3' and 5' ends of the cDNAs via 3' and 5' RACE analysis, 3 full-length genes designated as CL-ETR1, CL-ERS1, and CL-ETR2 were obtained. Upon cloning and sequencing, it was determined that the CL-ETR1, CL-ERS1, and CL-ETR2 …