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A Statistical Approach For Evaluation Of Pcr Results To Improve The Practical Limit Of Quantification (Loq) Of Gmo Analyses (Simquant), Torstein Tengs
A Statistical Approach For Evaluation Of Pcr Results To Improve The Practical Limit Of Quantification (Loq) Of Gmo Analyses (Simquant), Torstein Tengs
Dr. Torstein Tengs
The predominant approach for quantification of genetically modified organisms (GMO) is the application of quantitative real-time PCR. However, for a large number of processed food and feed products, this approach is unsuitable, because they contain low amounts (mass) of amplifiable DNA. Here we present a novel approach, ‘‘Single molecule quantification’’ (SIMQUANT) for GMO quantification of samples with extremely low amounts of DNA. The approach is based on statistics and application of multiple qualitative parallel PCRs. Here the qualitative PCRs were done using real-time PCR setup, but this is not a requirement. The difference is that the quantitative real-time PCR requires …