Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Publication Type
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Effects Of Curcumin And Ursolic Acid On The Mitochondrial Coupling Efficiency And Hydrogen Peroxide Emission Of Intact Skeletal Myoblasts, Daniel J. Tueller
Effects Of Curcumin And Ursolic Acid On The Mitochondrial Coupling Efficiency And Hydrogen Peroxide Emission Of Intact Skeletal Myoblasts, Daniel J. Tueller
Theses and Dissertations
Curcumin is a natural compound that improves blood glucose management. While some evidence from isolated mitochondria indicates that curcumin uncouples electron transport from oxidative phosphorylation, the effects of curcumin on mitochondrial respiration and hydrogen peroxide emission in intact skeletal muscle cells are not known. By assessing rates of oxygen consumption, we demonstrated for the first time that curcumin (40 µM) reduced the mitochondrial coupling efficiency (percentage of oxygen consumption that supports ATP synthesis) of intact skeletal muscle cells. A 30-minute incubation with curcumin decreased mitochondrial coupling efficiency by 17.0 ± 0.4% relative to vehicle (p < 0.008). Curcumin also decreased the rate of hydrogen peroxide emission by 43 ± 13% compared to vehicle (p < 0.05). Analysis of cell respiration in the presence of curcumin revealed a 40 ± 4% increase in the rate of oxygen consumption upon curcumin administration (p < 0.05 compared to vehicle). In additional experiments, no difference in mitochondrial coupling efficiency was observed between vehicle- and curcumin-pretreated cells after permeabilization of cell membranes (p > 0.7). The possibility of synergistic …
Effects Of Muscle Cooling On Ampk And Protein Synthesis In Skeletal Muscle, Kyle Lyons, David Thomson, Pdbio
Effects Of Muscle Cooling On Ampk And Protein Synthesis In Skeletal Muscle, Kyle Lyons, David Thomson, Pdbio
Journal of Undergraduate Research
AMP-activated protein kinase (AMPK) is an intracellular protein that regulates many signaling responses including the mechanistic target of rapamycin (mTOR) pathway, which increases protein synthesis1. Recent research suggests that cold-water immersion of the leg activates AMPK in skeletal muscle. It is not known whether cold directly activates AMPK in skeletal muscle or if the aforementioned results are due to systemic or hormonal responses. Furthermore, the direct effect of cooling on protein synthesis-related signaling in skeletal muscle has not been established. If cooling affects the activation of AMPK, then it could also affect mTOR. Therefore, the purpose of this study was …
Lkb1 Regulation Of High-Fat Diet-Induced Adaptation In Mouse Skeletal Muscle, Ting Chen
Lkb1 Regulation Of High-Fat Diet-Induced Adaptation In Mouse Skeletal Muscle, Ting Chen
Theses and Dissertations
Ad libitum high-fat diet (HFD)-induced obesity leads to insulin resistance in skeletal muscle, altered gene expression, and altered growth signaling, all of which contributes to pathological changes in metabolism. Liver kinase B1 (LKB1) is an important metabolism regulator. The purpose of this dissertation was to understand how knocking out LKB1 influences HFD induced adaptations in mouse skeletal muscle. To do so, control and skeletal muscle LKB1 knock-out (LKB1-KO) mice were put on either standard diet (STD) or HFD for 1 week or 14 weeks, or put on the HFD for 14 weeks and then switched to STD for 1 week …