Life Sciences Commons^™

Effects Of Phytohormone On Lateral Bud Elongation And Acidinvertase Activity During The Regrowth Of Sorghum Bicolor M., H Nojima, Y Takasaki, A Isoda

IGC Proceedings (1997-2023)

The relationships of phytohormone and sucrose metabolism in the stem ofoesorghum after cutting were studied using the stem disks treated with various plantoegrowth regulators The release of buds after incubating was accelerated by 6-benzyladenoprine riboside (6- BAR) treatment. But, on abcisic acid (ABA) andoeindole acetic acid (IAA ) treatment the release of buds was retarded. The length ofoeelongated buds on all hormonal treatments was shorter than the non-treated at the end of incubated period tested. Invertase activities were stimulated on all hormonal treatments. On 6-BAR treatment, invertase activities were the highest. But there were no relationships between invertase activities and …

Sunn Hemp As An Alternative Forage In The Texas High Plains Region, S. A. O’Shaughnessy, H. M. Mosqueda, D. K. Brauer

IGC Proceedings (1997-2023)

Forage production in the Texas High Plains, a semi-arid region, is critical to sustain the local cattle industry. However, the main source of water for irrigation is the highly depleted Ogallala Aquifer, making forage crop water productivity (CWP) of high importance. In this one-year study, three types of forages were cultivated under deficit irrigation treatments of 80% and 50% of full crop water use. The forages were: a non-brown midrib forage sorghum; a legume, sunn hemp; and a sorghum/sunn hemp mix. The experiment was conducted in Bushland, Texas in 2019. Treatment plots were arranged in a split plot design with …

Smallholder Zebu And Forage Production Development In Central Madagascar, L. Randriamanalina, M. S. Vorontsova, M. T. Rajaonah, O. P. Nanjarisoa, C. E. R. Lehmann, D. Miharinjanahary, H. N. S. Ratovoarinjaka, A. Bendrainy, C. Rijaniaina, A. M. Rabendrina, D. Rabeharison, B. F. Rakotozafy, F. Rakotoarison, D. Tahirinirainy, T. Randriamboavonjy, H. Ralimanana, J. Raharimampionona, C. Birkinshaw, W. Truter

IGC Proceedings (1997-2023)

Poverty in the Central Highlands of Madagascar is partly driven by inefficient exploitation of native forages and poor livestock nutrition. Zebus are of importance as cultural symbols, but this tradition has grown disconnected from agricultural policy. This project is working with three central highland communities near the protected areas of Itremo, Ibity and Ankafobe, to boost rangeland productivity and trial management methods which will support key forage grasses and improve livestock nutrition. A severe nitrogen deficiency compounded by extremely acidic soil conditions and low phosphorus is observed across the three sites. The lowest grazing capacity of an estimated 0.7 livestock …

Inhibiting Dhurrin Biosynthesis Effects On Stocker Cattle Daily Gains, S. M. Gruss, K. D. Johnson, R. P. Lemenager, M. R. Tuinstra

IGC Proceedings (1997-2023)

Climate change has made resilient crops more valuable in many agricultural production systems. Sorghum is an important resilient grain and forage crop due to its high drought tolerance and ability to thrive in low-N environments. All current commercial sorghum varieties produce the cyanogenic glucoside dhurrin. Cell maceration causes the conversion of dhurrin to hydrogen cyanide (HCN), which is toxic to animals. Toxicity symptoms range from labored breathing and convulsions to death within minutes. The dhurrin biosynthesis pathway was altered to inhibit dhurrin production by a mutation that inactivated CYP79A1, the first enzyme in the pathway. The dhurrin-free phenotype eliminates the …

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …

A Leaf-Level Spectral Library To Support High-Throughput Plant Phenotyping: Predictive Accuracy And Model Transfer, Nuwan K. Wijewardane, Huichun Zhang, Jinliang Yang, James C. Schnable, Daniel P. Schachtman, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Leaf-level hyperspectral reflectance has become an effective tool for high-throughput phenotyping of plant leaf traits due to its rapid, low-cost, multi-sensing, and non-destructive nature. However, collecting samples for model calibration can still be expensive, and models show poor transferability among different datasets. This study had three specific objectives: first, to assemble a large library of leaf hyperspectral data (n=2460) from maize and sorghum; second, to evaluate two machine-learning approaches to estimate nine leaf properties (chlorophyll, thickness, water content, nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur); and third, to investigate the usefulness of this spectral library for predicting external datasets …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Ensiling Of Tannin-Containing Sorghum Grain, E. M. Ott, Y. Acosta Aragón, M. Gabel

IGC Proceedings (1997-2023)

Sorghum is known as important feed-stuff in tropical regions where rainfall is insufficient for the cultivation of maize. Furthermore, those sorghum cultivars rich in tannins are naturally protected to a certain extent against bird damage, insect pests and moulds. Nevertheless, tannins impair the feed quality. Thus, the objectives of this study were to investigate whether ensiling could be a suitable preservation method for sorghum grain originally rich in tannins and if it is possible to reduce tannin content during fermentation.

Comparison Of Different Maize Hybrids Cultivated And Fermented With Or Without Sorghum, Sz. Orosz, Z. Bellus, Zs. Kelemen, E. Zerényi, J. Helembai

IGC Proceedings (1997-2023)

In Hungary our key forage crop is silage maize, however, the joint growing of maize and sorghum is increasingly important in arid regions. The reason is, that sorghum varieties tolerate well the various ecological stresses (drought). The joint growing of maize and sorghum varieties has several advantages and disadvantageous in respect of yields, safety of production, fermentability of the crop and nutrient content of the silage. The basis of realising the complementary qualities of the two crops and of the successful joint growing and preservation is the suitable pairing of hybrid varieties.

Assessment Of Bacterial Inoculant Delivery Methods For Cereal Crops, Yen Ning Chai, Stephanie Futrell, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was …

Microbial Community Field Surveys Reveal Abundant Pseudomonas Population In Sorghum Rhizosphere Composed Of Many Closely Related Phylotypes, Dawn Chiniquy, Elle M. Barnes, Jinglie Zhou, Kyle Hartman, Xiaohui Li, Amy Sheflin, Ellen Marsh, Jessica Prenni, Adam M. Deutschbauer, Daniel P. Schachtman, Susannah G. Tringe

Department of Agronomy and Horticulture: Faculty Publications

While the root-associated microbiome is typically less diverse than the surrounding soil due to both plant selection and microbial competition for plant derived resources, it typically retains considerable complexity, harboring many hundreds of distinct bacterial species. Here, we report a time-dependent deviation from this trend in the rhizospheres of field grown sorghum. In this study, 16S rRNA amplicon sequencing was used to determine the impact of nitrogen fertilization on the development of the root-associated microbiomes of 10 sorghum genotypes grown in eastern Nebraska. We observed that early rhizosphere samples exhibit a significant reduction in overall diversity due to a high …

Voxel Carving-Based 3d Reconstruction Of Sorghum Identifies Genetic Determinants Of Light Interception Efficiency, Mathieu Gaillard, Chenyong Miao, James Schnable, Bedrich Benes

Department of Agronomy and Horticulture: Faculty Publications

Changes in canopy architecture traits have been shown to contribute to yield increases. Optimizing both light interception and light interception efficiency of agricultural crop canopies will be essential to meeting the growing food needs. Canopy architecture is inherently three-dimensional (3D), but many approaches to measuring canopy architecture component traits treat the canopy as a two-dimensional (2D) structure to make large scale measurement, selective breeding, and gene identification logistically feasible. We develop a high throughput voxel carving strategy to reconstruct 3D representations of sorghum from a small number of RGB photos. Our approach builds on the voxel carving algorithm to allow …

Introduction To Sorghum Paper Production, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Sorghum is a tall grass used for many commercial products such as fodder and syrup. The 10 to 15 feet stalk of the plant has lower lignin than wood and provides a fiber length of 2.31 mm in the outer covering and 1.38 mm for the leaf. Sorghum fiber makes a high quality, strong paper suitable for printing, packaging and paperboard.

Engineering Linear, Branched-Chain Triterpene Metabolism In Monocots, Chase Kempinski, Zuodong Jiang, Garrett Zinck, Shirley J. Sato, Zhengxiang Ge, Thomas E. Clemente, Joseph Chappell

Department of Agronomy and Horticulture: Faculty Publications

Triterpenes are thirty-carbon compounds derived from the universal five-carbon prenyl precursors isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Normally, triterpenes are synthesized via the mevalonate (MVA) pathway operating in the cytoplasm of eukaryotes where DMAPP is condensed with two IPPs to yield farnesyl diphosphate (FPP), catalyzed by FPP synthase (FPS). Squalene synthase (SQS) condenses two molecules of FPP to generate the symmetrical product squalene, the first committed precursor to sterols and most other triterpenes. In the green algae Botryococcus braunii, two FPP molecules can also be condensed in an asymmetric manner yielding the more highly branched triterpene, botryococcene. Botryococcene …

Biomass Production Of Herbaceous Energy Crops In The United States: Field Trial Results And Yield Potential Maps From The Multiyear Regional Feedstock Partnership, Dokyoung Lee, Ezra Aberle, Eric K. Anderson, William Anderson, Brian S. Baldwin, David D. Baltensperger, Michael Barrett, Jurg Blumenthal, Stacy Bonos, Joe Bouton, David I. Bransby, Charlie Brummer, Pane S. Burks, Chengci Chen, Christopher Daly, Jose Egenolf, Rodney L. Farris, John H. Fike, Roch E. Gaussoin, John R. Gill, Kenneth Gravois, Michael D. Halbleib, Anna Hale, Wayne Hanna, Keith Harmoney, Emily A. Heaton, Ron W. Heiniger, Lindsey Hoffman, Chang O. Hong, Gopal Kakani, Robert Kallenbach, Bisoondat Macoon, James C. Medley, Ali Missaoui, Robert B. Mitchell, Ken J. Moore, Jesse I. Morrison, Gary N. Odvody, Jonathan D. Richwine, Richard Ogoshi, Jimmy Ray Parrish, Lauren Quinn, Ed Richard, William L. Rooney, J. Brett Rushing, Ronnie Schnell, Matt Sousek, Scott A. Staggenborg, Thomas Tew, Goro Uehara, Donald R. Viands, Thomas Voigt, David Williams, Linda Williams, Lloyd Ted Wilson, Andrew Wycislo, Yubin Yang, Vance Owens

Department of Agronomy and Horticulture: Faculty Publications

Current knowledge of yield potential and best agronomic management practices for perennial bioenergy grasses is primarily derived from small-scale and short-term studies, yet these studies inform policy at the national scale. In an effort to learn more about how bioenergy grasses perform across multiple locations and years, the U.S. Department of Energy (US DOE)/Sun Grant Initiative Regional Feedstock Partnership was initiated in 2008. The objectives of the Feedstock Partnership were to (1) provide a wide range of information for feedstock selection (species choice) and management practice options for a variety of regions and (2) develop national maps of potential feedstock …

A Comprehensive Analysis Of Alternative Splicing In Paleopolyploid Maize, Wenbin Mei, Sanzhen Liu, James C. Schnable, Cheng-Ting Yeh, Nathan M. Springer, Patrick S. Schnable, William Brad Barbazuk

Department of Agronomy and Horticulture: Faculty Publications

Identifying and characterizing alternative splicing (AS) enables our understanding of the biological role of transcript isoform diversity. This study describes the use of publicly available RNA-Seq data to identify and characterize the global diversity of AS isoforms in maize using the inbred lines B73 and Mo17, and a related species, sorghum. Identification and characterization of AS within maize tissues revealed that genes expressed in seed exhibit the largest differential AS relative to other tissues examined. Additionally, differences in AS between the two genotypes B73 and Mo17 are greatest within genes expressed in seed. We demonstrate that changes in the level …

Validation Of Qtl Mapping And Transcriptome Profiling For Identification Of Candidate Genes Associated With Nitrogen Stress Tolerance In Sorghum, Malleswari Gelli, Anji Reddy Konda, Kan Liu, Chi Zhang, Thomas E. Clemente, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Quantitative trait loci (QTLs) detected in one mapping population may not be detected in other mapping populations at all the time. Therefore, before being used for marker assisted breeding, QTLs need to be validated in different environments and/or genetic backgrounds to rule out statistical anomalies. In this regard, we mapped the QTLs controlling various agronomic traits in a recombinant inbred line (RIL) population in response to Nitrogen (N) stress and validated these with the reported QTLs in our earlier study to find the stable and consistent QTLs across populations. Also, with Illumina RNA-sequencing we checked the differential expression of …

Expression Of The Maize Dof1 Transcription Factor In Wheat And Sorghum, Pamela A. Pena, Truyen Quach, Shirley Sato, Zhengxiang Ge, Natalya Nersesian, Taity Changa, Ismail M. Dweikat, Madhavan Soundararajan, Tom E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen is essential for plant growth and development. Improving the ability of plants to acquire and assimilate nitrogen more efficiently is a key agronomic parameter that will augment sustainability in agriculture. A transcription factor approach was pursued to address improvement of nitrogen use efficiency in two major commodity crops. To this end, the Zea mays Dof1 (ZmDof1) transcription factor was expressed in both wheat (Triticum aestivum) and sorghum (Sorghum bicolor) either constitutively, UBI4 promoter from sugarcane, or in a tissue specific fashion via the maize rbcS1 promoter. The primary transcription activation target of ZmDof1 …

Mapping Qtls And Association Of Differentially Expressed Gene Transcripts For Multiple Agronomic Traits Under Different Nitrogen Levels In Sorghum, Malleswari Gelli, Sharon E. Mitchell, Kan Liu, Thomas E. Clemente, Donald P. Weeks, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important C₄ crop which relies on applied Nitrogen fertilizers (N) for optimal yields, of which substantial amounts are lost into the atmosphere. Understanding the genetic variation of sorghum in response to limited nitrogen supply is important for elucidating the underlying genetic mechanisms of nitrogen utilization.

Results: A bi-parental mapping population consisting of 131 recombinant inbred lines (RILs) was used to map quantitative trait loci (QTLs) influencing different agronomic traits evaluated under normal N (100 kg.ha⁻¹ fertilizer) and low N (0 kg.ha⁻¹ fertilizer) conditions. A linkage map spanning 1614 cM was developed …

Grain Sorghum Yield Response To Water Availability, J. P. Broeckelman, E. A. Adee, G. J. Kluitenberg, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

Yield effects of irrigation on sorghum and corn were compared, but this report is merely focused on the sorghum phase of the crop rotation. Mean yield for irrigated sorghum was 168 bu/a, whereas dryland yield was 145 bu/a. The latter represents a yield improvement of 24 bu/a, an increase of approximately 2 bu/a per unit (in.) of water applied (considering a total of 11 in. of water applied in the irrigation block).

The irrigated sorghum used a mean of 7.8 in. more water than the dryland, which suggests that the dryland sorghum consumed 3.4 in. more water from the soil …

Grain Sorghum Yield Response To Water Availability, J. P. Broeckelman, G. J. Kluitenberg, K. Roozeboom, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

Yield effects of irrigation on sorghum and corn were compared, focusing only on the grain sorghum phase. Average water use for irrigation was 22 in., and dryland sorghum used 17 in. Average yields based on 12.5% grain moisture for dryland and irrigated sorghum were similar, with 138 bu/a for the irrigated and 142 bu/a for the dryland environment. Irrigated sorghum yields were similar, but in dryland, the Pioneer 84G62 hybrid yielded 149 bu/a, a 10 bu/a increase over Pioneer 84Y50 and DKS 53-67 hybrids, which yielded 139 bu/a and 138 bu/a, respectively. Although there was a difference in the yield …

Balanced Nutrition And Crop Production Practices For Closing Grain Sorghum Yield Gaps, B. Mchenry, E. A. Adee, P. V. Vara Prasad, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

A field experiment was conducted at the East Central Kansas Experiment Field near Ottawa, KS, and at the Kansas River Valley Experiment Field near Rossville, KS, in the summer of 2014 to evaluate diverse cropping systems approaches on closing sorghum yield gaps. Yield gaps can be understood as the difference between maximum yield and attainable on-farm yields. The factors that were tested include narrow row spacing; plant population; balanced nutrition practices, including various timings of nitrogen, phosphorus, and potassium (NPK) and micronutrient applications; crop protection with fungicide and insecticide applications; plant growth regulator effects; and the use of precision ag …

Balanced Nutrition And Crop Production Practices For Closing Grain Sorghum Yield Gaps, B. Mchenry, P. V. Vara Prasad, I. A. Ciampitti

Kansas Agricultural Experiment Station Research Reports

A field experiment was conducted at the North Central Kansas Experiment Field near Scandia, KS, in the summer of 2014 to evaluate diverse cropping systems approaches to closing sorghum yield gaps. Yield gaps can be understood as the difference between maximum and attainable on-farm yields. The approach taken in this project is system wide, rather than focusing on one factor and its interaction. The factors that were tested include narrow row spacing; plant population; balanced nutrition practices, including various timings of nitrogen, phosphorus, and potassium (NPK) and micronutrient applications; crop protection with fungicide and insecticide applications; plant growth regulator effects; …

Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress.

Results: Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three …

Automated Conserved Non-Coding Sequence (Cns) Discovery Reveals Differences In Gene Content And Promoter Evolution Among Grasses, Gina Marie Turco, James C. Schnable, Brent S. Pedersen, Michael Freeling

Department of Agronomy and Horticulture: Faculty Publications

Conserved non-coding sequences (CNS) are islands of non-coding sequence that, like protein coding exons, show less divergence in sequence between related species than functionless DNA. Several CNSs have been demonstrated experimentally to function as cis-regulatory regions. However, the specific functions of most CNSs remain unknown. Previous searches for CNS in plants have either anchored on exons and only identified nearby sequences or required years of painstaking manual annotation. Here we present an open source tool that can accurately identify CNSs between any two related species with sequenced genomes, including both those immediately adjacent to exons and distal sequences separated by …

Feed The Future Releases Two New Sorghum Varieties In Nicaragua, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

de Tecnologia Agropecuaria (INTA), the Collaborative Research Support Program for Sorghum, Millet and Other Grains (INTSORMIL) has released two new varieties of sorghum in Nicaragua that will be used for forage (plant material eaten by grazing livestock).

These new varieties exhibit the “brown midrib” trait (bmr), which has been used for many years by sorghum producers in the United States. The bmr trait increases the digestibility of sorghum by reducing the amount of lignin, a chemical compound found in the cell walls of plants. The more digestible sorghum is for the livestock that consume it, the higher the quality of …

Use Of Cti Grinders For Sorghum Flour Production And Utilization: Final Report, Vilma Ruth Calderón De Zacatares, Lily Marisol López, Kris Emillee Duville, Carla Elizabeth Bermúdez

INTSORMIL Scientific Publications

The use of sorghum for human consumption to add value to the crop and increase its profitability, especially for small and medium producers, has contributed to food security and nutrition in El Salvador. CENTA is promoting the cultivation of sorghum as an alternative to food production in rural and urban areas. Since 2010 when wheat prices lower again, sorghum was no more an alternative to replace wheat in certain types of food, but is being used in more stable markets, as people allergic to gluten, people with diabetes and to correct nutritional deficiencies such as iron deficiency anemia because of …

Cambio Climático Reduciría Cultivos: El País Vería Una Baja De Entre Un 20% Y 50% En El Rendimiento Del Frijol, Maíz Y Sorgo, Por El Incremento En Las Temperaturas, Según Un Informe De La Universidad Estatal De Kansas, Rosa María Pastrán

INTSORMIL Impacts and Bulletins

El aumento de la temperatura que se producirá en los próximos 50 años, a consecuencia del cambio climático, provocaría una reducción en la producción de granos básicos en El Salvador, según un estudio del Departamento de Agronomía de la Universidad Estatal de Kansas, Estados Unidos.

En el caso de El Salvador, la investigación encontró que la temperatura actual incrementará entre 3 y 5 grados centígrados (°C). Este aumento afectará, de manera progresiva, el rendimiento de cultivos como frijol, maíz y sorgo, de acuerdo con lo que explicó el profesor Vara Prasad, quien presentó el estudio en el país.

El cultivo …

Separation Of Alcohol Soluble Sorghum Proteins Using Non-Porous Cation-Exchange Columns, Deidre L. Blackwell, Scott R. Bean

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Kafirins, the storage proteins and major protein of the cereal grain sorghum, play an important nutritional role for millions of people in parts of Africa and Asia. Kafirins are non-water soluble, being soluble only in the presence of detergents or aqueous alcohol mixtures and are among the most hydrophobic of the cereal proteins. Limited M_w heterogeneity of kafirins reduces their resolution when separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Charge based separation techniques have been shown to have improved resolution of kafirins, but due to the nature of their solubility, ion-exchange (IE)-HPLC has not been widely used to …