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The Saccharomyces Cerevisiae Ubiquitin-Proteasome System, Mark Hochstrasser, Phoebe Johnson, Cassandra Arendt, Alexander Amerik, Sowmya Swaminathan, Rob Swanson, Shyr-Jiann Li, Jeffrey Laney, Robin Pals-Rylaarsdam, Jonathan Nowak, Pamela Connerly, M. Yanagida, K. Nasmyth, M. Tyers, R. Hunt, J. Diffley
The Saccharomyces Cerevisiae Ubiquitin-Proteasome System, Mark Hochstrasser, Phoebe Johnson, Cassandra Arendt, Alexander Amerik, Sowmya Swaminathan, Rob Swanson, Shyr-Jiann Li, Jeffrey Laney, Robin Pals-Rylaarsdam, Jonathan Nowak, Pamela Connerly, M. Yanagida, K. Nasmyth, M. Tyers, R. Hunt, J. Diffley
Rob Swanson
Our studies of the yeast ubiquitin-proteasome pathway have uncovered a number of general principles that govern substrate selectivity and proteolysis in this complex system. Much of the work has focused on the destruction of a yeast transcription factor, MAT alpha 2. The alpha 2 protein is polyubiquitinated and rapidly degraded in alpha-haploid cells. One pathway of proteolytic targeting, which depends on two distinct endoplasmic reticulum-localized ubiquitin-conjugating enzymes, recognizes the hydrophobic face of an amphipathic helix in alpha 2. Interestingly, degradation of alpha 2 is blocked in a/alpha-diploid cells by heterodimer formation between the alpha 2 and a1 homeodomain proteins. The …