Life Sciences Commons^™

Functional Outcomes Of Noninvasive Sensory Feedback In Upper Limb Prostheses: A Meta-Analysis, Libby Caldwell

UNO Student Research and Creative Activity Fair

Noninvasive sensory feedback applies vibration or pressure to the residual limb and is thought to improve the functionality of upper limb prostheses. However, the efficacy of this approach has not been sufficiently investigated, particularly regarding actual amputee efficacy. The purpose of this study was to evaluate the functional benefits of noninvasive sensory feedback in upper limb prostheses through a meta-analysis. Electronic databases (MEDLINE, Web of Science, and PubMed) were searched up to October 2022 using the following search terms: (prosthesis OR prosthetic) AND (hand OR arm OR forearm OR transradial OR “upper limb” OR “upper extremity) AND (tactile OR haptic …

Ingredient Classification Using Food Ontology, Ricky Flores

UNO Student Research and Creative Activity Fair

A food label provides some of the most crucial information for a food product. The food label is a key resource for many health-conscious consumers for understanding ingredients. It is also vital for individuals to avoid food allergens or help patients follow dietary recommendations. While the food labels in the United States are regulated by the Food and Drug Administration (FDA) many labels contain additional information or statements that are not regulated. Moreover, the food label may be complex or contain terminology that the layperson may not understand. Evidence has indicated that consumers often find nutrition labels confusing, especially when …

Comparison Of Gray Squirrel (Sciurus Carolinensis) And Fox Squirrel (Sciurus Niger) In Order To Explore Genes That Confer A Fitness Advantage, Joshua Franzen

UNO Student Research and Creative Activity Fair

Mutations in the MC1R gene is associated with melanism, or black fur, and the presence of a 24 base pair deletion in the MC1R gene of fox and gray squirrels suggests a shared ancestry between the two species. This could be due to a mutation in a common ancestor or previous mating between species. Evidence has shown that the most likely cause for the presence of MC1R in both species is previous mating between species. If this did occur, then it is possible that other genes traveled between species along with the mutated MC1R gene. Conserved genes shared between species …

Variation In Genes Responsible For Melanism In Sciurus Carolinensis And Sciurus Niger, Ibrahim Hussain

UNO Student Research and Creative Activity Fair

During the past few years, the population of melanistic squirrels in Nebraska has increased. These color variations have been associated with variation in the Melanocortin 1 Receptor (MC1R) and Agouti Signaling Protein (ASIP) genes, which are responsible for pigment production. DNA was isolated from 3 grey and 46 fox squirrels collected from Eastern Nebraska; 8 of the fox squirrels were melanistic in color. Primers were designed to amplify and sequence MC1R and three portions of ASIP to examine the extent of variation in these genes in local squirrels. The sequences were aligned to the grey squirrel reference …

Exploring Anti-Schistosoma Analog Sa01, Sarah Alsuleiman

UNO Student Research and Creative Activity Fair

Schistosomiasis is a waterborne parasitic worm infection able to infect in the larval stage through direct skin penetration. This disease affects approximately 270 million people worldwide and is widespread in tropical as well as subtropical regions with higher distribution in areas lacking sufficient sanitation and safe drinking water. Although some possible alternatives are emerging, currently, the most effective drug treatment is praziquantel (PZQ). However, PZQ is only effective against the adult stage of the worm, allowing juvenile worms to progress in the infection. Furthermore, Schistosoma is developing resistance to this drug as reduced efficacy has been noted. As a result, …

Insights Into Viral Genome Function Through Comparative Structural Analysis, Lydia Phillips

UNO Student Research and Creative Activity Fair

Enteroviruses are single stranded RNA viruses which have caused many public health concerns, particularly in children. These viruses are responsible for polio, hand, foot, and mouth disease, many polio-like neurological diseases, and the common cold. The enterovirus called Coxsackievirus B3 (CVB3), a close relative to poliovirus, has been shown responsible for severe human disease, including pancreatitis, myocarditis, and type 1 diabetes. A unique advantage of studying CVB3 is the existence of a naturally occurring strain (CVB3/GA) which displays no known pathogenicity. Earlier comparative genomic studies have shown that the primary difference between avirulent CVB3/GA and the virulent strains of CVB3 …

Predictors Of Testosterone In Male African Elephants (Loxodonta Africana), Kaitlyn Campbell

UNO Student Research and Creative Activity Fair

Male African elephant (Loxodonta africana) populations in zoos suffer from a range of reproductive complications. In particular, insufficient testosterone production can have a detrimental effect on spermatogenesis and fertility. Few studies have explored the potential social, environmental, physiological and physical factors that influence bull testosterone levels in a zoo environment. The present study will collect endocrine and metabolic information from weekly fecal and blood serum samples taken over the course of the six-month study period. Social and environmental data will be collected in a survey provided to the institutions. Results will highlight the key factors contributing to fluctuations in testosterone …

High-Throughput Sequencing Pipeline Tool Analysis - Staphylococcus Aureus, Justin Fay

UNO Student Research and Creative Activity Fair

In this project, I compared the tools used at each step of the high-throughput sequencing pipeline used to detect single-nucleotide polymorphisms (SNP’s). There are 11 sequences I utilized from my mentor, Dr. Kate Cooper, on this project (pertaining to Staphylococcus aureus) and I used those in the sequencing pipeline. I evaluated different tools by using different ones at different steps of the pipeline. By doing this, I was able to see how close or far the outputs are by only changing one factor. It is also important to note that, with each online tool comes its own sets of parameters. …

Combined Berry Extract And Bromelain Supplement Improves Blood Pressure, Endothelial Function, And Skeletal Muscle Oxygenation Status In Healthy Young Adults, Tesean K. Wooden

UNO Student Research and Creative Activity Fair

Cardiovascular disease (CVD) is associated with the manifestation of increased levels of oxidative stress and inflammation. Reactive oxygen species (ROS) production may cause damage to the vasculature that can lead to diseases, such as diabetes, hypertension and atherosclerosis. Anthocyanins are the largest class of flavonoid that are found in vegetables and fruit. Therefore, the combination of anthocyanins and bromelain may be an ideal intervention for improving or maintaining cardiovascular health. We sought to examine the effects of this combination of powerful antioxidants within healthy young adults.

Young healthy adults (n=18) volunteered to participate in this study. Participants were randomly assigned …

Juvenile Hormone Mediation In An Insect With Parental Care Behavior, Jessica M. Rodino

UNO Student Research and Creative Activity Fair

Juvenile hormone (JH) is a well-known catalyst for hormonal processes in insects. However, the role of JH in insects that exhibit parental behavior is unknown. We investigated the influence of JH on parental behavior in the burying beetle (Nicrophorus orbicollis). In the first experiment, we manipulated the JH production of females via the administration of varying doses of fluvastatin sodium immediately following oviposition. We found that with increasing fluvastatin dosage, the total mass of offspring and number of offspring decreased while at the same time less of the food source was consumed. These results suggest a link between …

The Effect Of Sensory Input On The Temporal Structure Of Center Of Pressure In Stroke Survivors, Samantha Chong

UNO Student Research and Creative Activity Fair

Stroke is the leading cause of disability that affects 17 million people worldwide. Patients post-stroke suffer from maintaining balance because the brain may not be able to receive or process visual, vestibular and proprioceptive sensory information: all of which contribute towards maintaining stability. Information provided by vision is important for spatial orientation, as it develops an environmental perspective. Proprioception is detected through the stretching of tendons and surrounding tissue and is able to help the brain determine spatial location. The vestibular system provides a sense of balance detecting rotations and linear accelerations through the vestibulo-ocular reflex. The vestibular system is …

The Effects Of Stiffness Of A Prosthetic Shock-Absorbing Pylon On Mechanical Work During Walking, Jenny Anne Maun

UNO Student Research and Creative Activity Fair

Name: Jenny Anne Maun

Title: The effects of stiffness of a prosthetic shock-absorbing pylon on mechanical work during walking

Degree: Masters

Faculty Advisor: Kota Z. Takahashi, PhD

Co-authors: Steven A. Gard, PhD; Matthew J. Major, PhD

A prosthetic shock-absorbing pylon (SAP) is intended to attenuate impact forces, provide comfort, and reduce residual limb pain for prosthesis users. However, the effect of an SAP and its spring stiffness on the mechanical work done by the prosthetic limb during walking remains unclear. The purpose of this study: to investigate the effects of SAP spring stiffness and walking speed on the mechanical work …

Walking Adaptations To An Ankle Foot Orthosis In Individuals With Peripheral Artery Disease, Todd Leutzinger, Iraklis Pipinos, Jason Johanning, Mahdi Hassan, Holly Despiegelaere, Mukul Mukherjee, Sara Myers

UNO Student Research and Creative Activity Fair

Single Session Walking Adaptations to an Ankle Foot Orthosis in Patients with Claudication and Peripheral Artery Disease

Todd Leutzinger^{1, 2}, Iraklis Pipinos^{2, 3}, Jason Johanning^{2, 3}, Mukul Mukherjee¹, Sara Myers^{1, 2}

¹Department of Biomechanics, University of Nebraska at Omaha, Omaha, NE USA

²Nebraska and Western Iowa Veterans Affairs’ Medical Center, Omaha, NE USA

³Department of Surgery, University of Nebraska Medical Center, Omaha, NE USA

Email: tleutzinger@unomaha.edu

Peripheral artery disease (PAD) is an atherosclerotic disease affecting the arteries of the lower extremities [1]. The primary symptom of PAD is …

How Prosthetic Ankle Stiffness & Load Carriage Affect Metabolic Energy Expenditure During Walking, Erica A. Hedrick, Philippe Malcolm, Jason M. Wilken, Kota Z. Takahashi

UNO Student Research and Creative Activity Fair

The ankle structures play a key role in body support, forward propulsion and leg swing. One important property of the human ankle is its ‘quasi-stiffness’, or resistance to angular motion. The ankle joint stiffness can play a role in aiding the lower leg as its rocks over the foot. Human biological ankle stiffness changes in response to changes in the mechanical demands of walking (e.g. added load or changes in speed), using active muscle contractions. However, the role of ankle stiffness in regulating metabolic energy across walking conditions is unknown. Therefore, the purpose of this study is to determine how …

Pregnancy In Zoo-Managed African Elephants (Loxodonta Africana): Investigating Possible Hormonal And Metabolic Indicators Of Successful And Unsuccessful Pregnancies., Chelsi Marolf

UNO Student Research and Creative Activity Fair

Female African elephants (Loxodanta africana) in zoos in the United States currently have high rates of ovarian acyclicity and if they do become pregnant, the calf is often lost before or during birth. This issue likely stems from various metabolic and reproductive factors, including obesity, advanced age, and stress. While this is a complicated problem, analyzing certain hormones related to reproduction and nutrition in elephants might reveal biological indicators for successful and unsuccessful pregnancies in African elephants. The goal of this study is to create a range of hormone levels throughout a typical successful pregnancy, and to compare …

Isolating And Characterizing Novel Bacteriophages From Freshwater Samples, Mackenzie Conrin, Avery Misfeldt

UNO Student Research and Creative Activity Fair

Bacteria are responsible for many Healthcare-Associated infections each year and are evolving to become multidrug resistant (MDR). Bacteriophages, also called phages, are naturally occurring bacterial viruses. These phages infect and kill their bacterial hosts. It is estimated that there are 10 different phages, per bacterium. This makes phages the most abundant entity in the biosphere. Isolating and characterizing these naturally occurring bacterial killers offers the potential for strain specific treatment of MDR infections. Such treatments, coined Phage Therapy, can be used in the fight against evolving bacterial infections. Pseudomonads are a classification of bacteria that infect a variety of plants …

Meta-Analysis Of Characteristics In Upper Missouri River Fishes: Prediction Of Invasiveness, Steph Purcell

UNO Student Research and Creative Activity Fair

Invasive species are often considered a global threat due to their association with biodiversity loss and novel diseases. The Missouri-Mississippi River Watershed, including the Missouri River Basin, is particularly vulnerable to invasive species because of low species diversity following historic glaciation events. Management of invasive species is imperative in this watershed but continues to be challenging in that there are over 100 invasive species currently present in this region. The goal of this project is to identify characteristics associated with successful invasions that may assist in developing management strategies to reduce the negative outcomes caused by the establishment of invasive …

Effects Of Chronic Ethanol Exposure On Stress Coping Style And Genetic States Of Zebrafish (Danio Rerio), Alexander Goodman

UNO Student Research and Creative Activity Fair

Neurotransmitter systems are important in regulating the stress response. If a behavioral response is disproportional to a stressor it is characterized as anxiety-like behavior. Many anxiolytic compounds, such as ethanol, increase stressor engagement, but how these compounds interact with an organism on a neurogenetic level is less understood. In this study, I assessed the impact of chronic ethanol treatment on behavior and gene expression of GABA_Areceptors subunits on two strains of zebrafish. Each strain was selectively bred to display the proactive or reactive stress coping style where proactive individuals will actively engage a stressor more than reactive individuals. …

A Compartmental Network Model For The Spread Of Whooping Cough, Kimia Ameri

UNO Student Research and Creative Activity Fair

Outbreaks of pertussis have increased over the past few years, drawing the attention of health care providers. Understanding the transmission mechanisms of contagious disease is critically important, but depends on many intricate factors including pathogen and host environment, exposed population, and their activities. In this work, we try to improve upon the prediction model for the exposed population. The number of whooping cough reported cases in Nebraska between 2000-2017 was gathered. The standard SEIR model is used to predict the infected numbers. The results show that the Susceptible-Exposed-Infected-Recovered (SEIR) model prediction for the number of infected individuals is much higher …

Visual Contributions To Balance Control During Gait, Kyle Brozek

UNO Student Research and Creative Activity Fair

We propose to isolate the contributions of continuous OF to the orthogonal relationship between balance and gait control during treadmill walking using a series of conditions. Manipulating the direction of OF using a CAREN (Motek Medical, Amsterdam, Netherlands) virtual reality treadmill environment will isolate these visual contributions in human subjects as they walk. We plan to tease out the effect of OF direction on the relationship between gait and balance control by having healthy young adults perform a series of treadmill walking trials while immersed in a VR environment. This will be done by manipulating the direction of walking and …

Physiological Response To Exercise With Naturally-Occurring Carbon Dioxide Exposure, Megan Johnson

UNO Student Research and Creative Activity Fair

BACKGROUND: Carbon dioxide (CO₂) plays a normal part in gas exchange and acid-base equilibrium in the human body. With added stress or exposure, CO₂ levels can accumulate in the blood and tissues, causing hypercapnia. Research has shown that exposure to CO₂ at 4% or 40,000 ppm will cause cardio-respiratory changes, both at rest and during exercise. Lower levels may still cause changes under conditions of added stress, but this has not been elucidated. Preliminary data from our laboratory chamber suggests physiological changes at levels of CO₂ reaching 0.08% or 8,000 ppm during experiments with exercising …

Targeted Therapy For The Future: The Use Of Novel Antimicrobial Peptides Against P. Aeurginosa, Matthew Froid

UNO Student Research and Creative Activity Fair

Pathogenic bacteria, such as the gram-negative bacterium Pseudomonas aeruginosa, are becoming resistant to our current arsenal of antibiotics at an alarming rate. P. aeruginosa is a leading cause of nosocomial acquired infections and is a primary co-morbidity in patients with compromised immune systems. One potential source of new antibiotic agents is antimicrobial peptides. Antimicrobial peptides (AMPs) are small proteins, and some have shown a high degree of efficacy and broad-spectrum activity against both Gram-positive and Gram-negative bacteria. An experimental AMP that has been developed by Dr. Wang at UNMC, DASamp2, has shown to be effective against virulent bacteria, including P. …

Precision Medicine: Bioinformatics Assists In Finding Accurate Treatment For Her2+ Breast Cancer In Humans, Elizabeth Russman

UNO Student Research and Creative Activity Fair

Human epidermal growth factor receptor 2 (HER2) is a gene located on chromosome 17q12 (Ferrari et al., 2016). A HER2 mutation is known to cause breast cancer, and is responsible for approximately 20% of all breast cancers.

In this project, HER2 positive breast cancer will be examined. The goal of this project is to understand the complicated gene, HER2, and how treatment needs to be more individualized and precise using bioinformatics.

Analysis Of Clustering Algorithms, Ethan Summers

UNO Student Research and Creative Activity Fair

In Bioinformatics, choosing the right algorithm for a problem is very important. Choosing the wrong algorithm or one that is less efficient can make or break a project. Analyzing algorithms beforehand is key. The goal of this project is to analyze three clustering algorithms for protein protein interaction networks and compare their function and results. A clustering algorithm takes a dataset, in this case a simulated PPI (protein-protein interaction) network and groups together similar data points based on some similarity criteria. It is important to know the difference between these algorithms to get the desired results.

Effects Of Rangeland Management On Milkweed Grazing And Monarch Conservation, Brittany Poynor

UNO Student Research and Creative Activity Fair

Cattle typically avoid consuming milkweed plants that contain high levels of toxic defense compounds, and therefore many people assume cattle avoid all milkweeds. However, common milkweed (Asclepias syriaca) contains only moderate levels of toxic compounds, and observations suggest that cattle regularly consume common milkweed and may even preferentially graze flowers and leaves of this species. These observations directly relate to efforts to add over one billion stems of milkweed to the central USA for monarch butterfly (Danaus plexippus) conservation. If cattle intentionally consume common milkweed and other milkweed species, and if certain management practices can reduce …

3d Prosthetics Effects On Standing Posture In Unilateral Upper Limb Deficient Children, Keaton Young

UNO Student Research and Creative Activity Fair

3D Prosthetics Effects on Standing Posture in Unilateral Upper Limb Deficient Children

Keaton Young

Department of Biomechanics, University of Nebraska at Omaha

Introduction

Upper Limb Reduction Deficiency (ULD) is a congenital disability that affects the upper limb, which the Center for Disease Control and Prevention estimates that nearly 1,500 babies are born with ULD in the United States yearly ^1,2. Prior research has focused on functional treatment of the upper limbs but has lacked observance of the effects that prosthetics and limb loss incur to the trunk. Therefore, the purpose of this study is to examine the acute effects …

Effects Of 7°C Environmental Temperature During A 3-Week Training Period, Robert J. Shute

UNO Student Research and Creative Activity Fair

Approximately three weeks is required for cold temperature acclimation to occur. However, the effects of cold temperature acclimation on fitness and performance remain unknown. Purpose: To determine the impact of cold environmental temperature on training adaptations, fitness measurements, and aerobic performance. Methods: Two groups of twelve inactive male subjects completed 1-hour of cycling in fourteen temperature acclimation trials of either a cold (7°C) or room temperature (20°C) environmental temperature. A performance trial and tolerance trial occurred pre- and post-acclimation. The performance trials assessed VO_2peak and body composition. Skin and core temperature were recorded during the 1-hour tolerance trials. Tympanic …

Data Analytics Pipeline For Rna Structure Analysis Via Shape, Quinn Nelson

UNO Student Research and Creative Activity Fair

Coxsackievirus B3 (CVB3) is a cardiovirulent enterovirus from the family Picornaviridae. The RNA genome houses an internal ribosome entry site (IRES) in the 5’ untranslated region (5’UTR) that enables cap-independent translation. Ample evidence suggests that the structure of the 5’UTR is a critical element for virulence. We probe RNA structure in solution using base-specific modifying agents such as dimethyl sulfate as well as backbone targeting agents such as N-methylisatoic anhydride used in Selective 2’-Hydroxyl Acylation Analyzed by Primer Extension (SHAPE). We have developed a pipeline that merges and evaluates base-specific and SHAPE data together with statistical analyses that provides confidence …

Large Scale Dynamical Model Of Macrophage/Hiv Interactions, Matthew M. Froid

UNO Student Research and Creative Activity Fair

Properties emerge from the dynamics of large-scale molecular networks that are not discernible at the individual gene or protein level. Mathematical models - such as probabilistic Boolean networks - of molecular systems offer a deeper insight into how these emergent properties arise. Here, we introduce a non-linear, deterministic Boolean model of protein, gene, and chemical interactions in human macrophage cells during HIV infection. Our model is composed of 713 nodes with 1583 interactions between nodes and is responsive to 38 different inputs including signaling molecules, bacteria, viruses, and HIV viral particles. Additionally, the model accurately simulates the dynamics of over …

Pilot Study Of Empathy In Adults, Libby Moberg

UNO Student Research and Creative Activity Fair

Empathy is of critical public health importance due to its association with relationship satisfaction and well-being (Davis & Oathout, 1987; Davis, 1983). There is growing evidence that bilingual individuals may have higher levels of empathy (Javor, 2016). One potential mechanism for this relationship is that bilingual individuals tend to have higher levels of executive functioning (Costa et al, 2008), which is linked to higher empathy because individuals are able to more easily adopt others’ perspectives. Previous studies examining this question have largely relied on self-report questionnaires assessing empathy as a general tendency (i.e., trait). No studies have examined differences in …