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Full-Text Articles in Life Sciences

Sam: A Tool For Measurement Of Moderate To Vigorous Physical Activity (Mvpa) In School Physical Education, Kampol Surapiboonchai, Steven Furney, Robert Reardon, James Eldridge, Tinker Murray Mar 2015

Sam: A Tool For Measurement Of Moderate To Vigorous Physical Activity (Mvpa) In School Physical Education, Kampol Surapiboonchai, Steven Furney, Robert Reardon, James Eldridge, Tinker Murray

James Eldridge

Int J Exerc Sci 5(2) : 127-135, 2012. Observational methods have been a primary methodology used by physical educators for assessing teacher and student behaviors in school physical education (PE) classes for over 30 years. Observational instruments traditionally used in PE are economical, but are time intensive and complicated. Recently national PE recommendations have been promoted to encourage practitioners to achieve ≥ 50% of PE class in moderate to vigorous physical activity (MVPA). The purpose of this preliminary study was to develop, validate, and test the reliability of the Simple Activity Measurement (SAM) instrument for assessing MVPA during school PE …


The Effects Of Vascular Occlusion Training On Respiratory Exchange Ratio And Energy Expenditure When Coupled With Cardiovascular Training, Justin Sprick, Richard Lloyd, James Eldridge Mar 2015

The Effects Of Vascular Occlusion Training On Respiratory Exchange Ratio And Energy Expenditure When Coupled With Cardiovascular Training, Justin Sprick, Richard Lloyd, James Eldridge

James Eldridge

International Journal of Exercise Science 8(1) : 57-64, 2015. Vascular occlusion training is a novel training modality that has received considerable attention in the exercise science literature. The current study is the first of its kind to look at the effects of vascular occlusion training on substrate utilization when combined with aerobic training. This study examined the effects of pairing a vascular occlusion training protocol with a bout of submaximal aerobic exercise on energy expenditure (EE) and fuel use during exercise. Subjects performed a 20 minute bout of sub-maximal aerobic exercise either exclusively, or after performing a body weight squat …


A Diabetic Adolescent Case Study: Use Of A Website In Combination With An Exercise Program To Increase Physical Activity, Danielle Buckley, Nicole Demilia, Stephanie Tanzi, Furong Xu, Linda Lamont Jun 2013

A Diabetic Adolescent Case Study: Use Of A Website In Combination With An Exercise Program To Increase Physical Activity, Danielle Buckley, Nicole Demilia, Stephanie Tanzi, Furong Xu, Linda Lamont

Linda S. Lamont Ph.D.

International Journal of Exercise Science 5(3) : 296-300, 2012. The purpose of this study was to report on the efficacy of a web-based technology support to encourage physical activity in children. This program was designed to promote physical activity and proper nutrition in the diabetic adolescent with a weekly meeting that consisted of a 30-minute educational session followed by 60-minutes of exercise. A specifically designed website was used as a support to this weekly supervised exercise program. Outcomes assessment included body mass index (BMI), muscle strength (grip strength, back and leg strength), flexibility, exercise self-efficacy, and physical activity participation (pedometer, …


Polymorphic Male Color Morphs Visualized With Steroids In Monomorphic Females: A Tool For Designing Analysis Of Sex-Limited Trait Inheritance, M Olsson, Mark Wilson, M Tobler Dec 2011

Polymorphic Male Color Morphs Visualized With Steroids In Monomorphic Females: A Tool For Designing Analysis Of Sex-Limited Trait Inheritance, M Olsson, Mark Wilson, M Tobler

Mark R Wilson

No abstract provided.


Polyelectrolyte Complex Materials Consisting Of Antibacterial And Cell-Supporting Layers, Khairul Mat Amin, Kerry Gilmore, Jake Matic, Stephen Poon, Mark Walker, Mark Wilson, Marc In Het Panhuis Dec 2011

Polyelectrolyte Complex Materials Consisting Of Antibacterial And Cell-Supporting Layers, Khairul Mat Amin, Kerry Gilmore, Jake Matic, Stephen Poon, Mark Walker, Mark Wilson, Marc In Het Panhuis

Mark R Wilson

The characterization of a polyelectrolyte complex material comprised of two biopolymers, a chitosan upper layer and a gellan gum under layer, is reported. It is shown that the upper layer of chitosan with incorporated levofloxacin displays an antibacterial activity, while the under layer of a gellan gum/TiO2 composite supports the growth of fibroblastic cells.


Sex-Specific Sod Levels And Dna Damage In Painted Dragon Lizards (Ctenophorus Pictus), Mats Olsson, Mo Healey, Cecile Perrin, Mark Wilson, Michael Tobler Dec 2011

Sex-Specific Sod Levels And Dna Damage In Painted Dragon Lizards (Ctenophorus Pictus), Mats Olsson, Mo Healey, Cecile Perrin, Mark Wilson, Michael Tobler

Mark R Wilson

When groups of individuals differ in activities that may influence the production of reactive molecules, such as superoxide, we expect selection to result in congruent upregulation of antioxidant production in the group(s) most at risk of suffering concomitant erosion of essential tissue and biomolecules, such as DNA. We investigate this in a (near) annual lizard species, the Australian painted dragon (Ctenophorus pictus), in which males and females have fundamentally different lifestyles, with males being overtly conspicuous and aggressive, whereas females are placid and camouflaged. When kept in identical conditions to females in captivity, males had higher levels of superoxide dismutase …


Predictors Of Telomere Content In Dragon Lizards, Cissy Ballen, Mo Healey, Mark Wilson, Michael Tobler, Mats Olsson Dec 2011

Predictors Of Telomere Content In Dragon Lizards, Cissy Ballen, Mo Healey, Mark Wilson, Michael Tobler, Mats Olsson

Mark R Wilson

Telomeres shorten as a consequence of DNA replication, in particular in cells with low production of telomerase and perhaps in response to physiological stress from exposure to reactive oxygen species, such as superoxide. This process of telomere attrition is countered by innate antioxidation, such as via the production of superoxide dismutase. We studied the inheritance of telomere length in the Australian painted dragon lizard (Ctenophorus pictus) and the extent to which telomere length covaries with masscorrected maternal reproductive investment, which reflects the level of circulating yolk precursor and antioxidant, vitellogenin. Our predictors of offspring telomere length explained 72 % of …


A Significant Component Of Ageing (Dna Damage) Is Reflected In Fading Breeding Colors: An Experimental Test Using Innate Antioxidant Mimetics In Painted Dragon Lizards, Mats Olsson, Michael Tobler, Mo Healey, Cecile Perrin, Mark Wilson Dec 2011

A Significant Component Of Ageing (Dna Damage) Is Reflected In Fading Breeding Colors: An Experimental Test Using Innate Antioxidant Mimetics In Painted Dragon Lizards, Mats Olsson, Michael Tobler, Mo Healey, Cecile Perrin, Mark Wilson

Mark R Wilson

A decade ahead of their time, von Schantz et al. united sexual selection and free radical biology by identifying causal links between deep-rooted physiological processes that dictate resistance to toxic waste from oxidative metabolism (reactive oxygen species, ROS), and phenotypic traits, such as ornaments.Ten years later, these ideas have still only been tested with indirect estimates of free radical levels (oxidative stress) subsequent to the action of innate and dietary antioxidants. Here, we measure net superoxide (a selection pressure for antioxidant production) and experimentally manipulate superoxide antioxidation using a synthetic mimetic of superoxide dismutase (SOD), Eukarion 134 (EUK). We then …


Net Superoxide Levels: Steeper Increase With Activity In Cooler Female And Hotter Male Lizards, Cissy Ballen, Mo Healey, Mark Wilson, Michael Tobler, Erik Wapstra, Mats Olsson Dec 2011

Net Superoxide Levels: Steeper Increase With Activity In Cooler Female And Hotter Male Lizards, Cissy Ballen, Mo Healey, Mark Wilson, Michael Tobler, Erik Wapstra, Mats Olsson

Mark R Wilson

"Ectotherms increase their body temperature in response to ambient heat, thereby elevating their metabolic rate. An often inferred consequence of this is an overall upregulation of gene expression and energetic expenditure, and a concomitant increased production of reactive oxygen species (e. g. superoxide) and, perhaps, a shortened lifespan. However, recent work shows that this may be a superficial interpretation. For example, sometimes a reduced temperature may in fact trigger up-regulation of gene expression. We studied temperature and associated activity effects in male and female Australian painted dragon lizards (Ctenophorus pictus) by allowing the lizards to bask for 4 h versus …


Basal Superoxide As A Sexspecific Immune Constraint, Michael Tobler, Mo Healey, Mark Wilson, Mats Olsson Dec 2010

Basal Superoxide As A Sexspecific Immune Constraint, Michael Tobler, Mo Healey, Mark Wilson, Mats Olsson

Mark R Wilson

No abstract provided.


Transcriptome Profiling Of A Tgf-Beta-Induced Epithelial-To-Mesenchymal Transition Reveals Extracellular Clusterin As A Target For Therapeutic Antibodies, A. Lenferink, C. Cantin, A. Nantel, E. Wang, Y. Durocher, M. Banville,, B. Paul-Roc, A. Marcil, Mark Wilson, M. O'Connor-Mccourt Dec 2009

Transcriptome Profiling Of A Tgf-Beta-Induced Epithelial-To-Mesenchymal Transition Reveals Extracellular Clusterin As A Target For Therapeutic Antibodies, A. Lenferink, C. Cantin, A. Nantel, E. Wang, Y. Durocher, M. Banville,, B. Paul-Roc, A. Marcil, Mark Wilson, M. O'Connor-Mccourt

Mark R Wilson

No abstract provided.


Polymorphic Ros Scavenging Revealed By Cccp In A Lizard, Mats Olsson, Mark Wilson, Caroline Isaksson, Tobias Uller Dec 2008

Polymorphic Ros Scavenging Revealed By Cccp In A Lizard, Mats Olsson, Mark Wilson, Caroline Isaksson, Tobias Uller

Mark R Wilson

Ingestion of antioxidants has been argued to scavenge circulating reactive molecules (e.g., free radicals), play a part in mate choice (by mediating access to this important resource), and perhaps increase life span. However, recent work has come to question these relationships. We have shown elsewhere in the polychromatic lizard, Ctenophorus pictus, that diet supplementation of carotenoids as antioxidants does not depress circulating natural reactive oxygen species (ROS) levels and leads to no corresponding improvement of color traits. However, a much stronger test would be to experimentally manipulate the ROS levels themselves and assess carotenoid-induced ROS depression. Here, we achieve this …


Variation In Levels Of Reactive Oxygen Species Is Explained By Maternal Identity, Sex And Body-Size-Corrected Clutch Size In A Lizard, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott Dec 2008

Variation In Levels Of Reactive Oxygen Species Is Explained By Maternal Identity, Sex And Body-Size-Corrected Clutch Size In A Lizard, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott

Mark R Wilson

No abstract provided.


Effect Of Statins On Serum Apolipoprotein J And Paraoxonase-1 Levels In Patients With Ischemic Heart Disease Undergoing Coronary Angiography, Mark Wilson, Dimitri Mikhailidis, Maria Poulakou, Despina Perrea, Kosmas Paraskevas, Dimitrios Iliopoulos, Serafim Tsitsilonis, Ioannis Vlachos, Sonia-Athena Karabina Dec 2007

Effect Of Statins On Serum Apolipoprotein J And Paraoxonase-1 Levels In Patients With Ischemic Heart Disease Undergoing Coronary Angiography, Mark Wilson, Dimitri Mikhailidis, Maria Poulakou, Despina Perrea, Kosmas Paraskevas, Dimitrios Iliopoulos, Serafim Tsitsilonis, Ioannis Vlachos, Sonia-Athena Karabina

Mark R Wilson

No abstract provided.


Carotenoid Intake Does Not Mediate A Relationship Between Reactive Oxygen Species And Bright Colouration: Experimental Test In A Lizard, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott Dec 2007

Carotenoid Intake Does Not Mediate A Relationship Between Reactive Oxygen Species And Bright Colouration: Experimental Test In A Lizard, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott

Mark R Wilson

No abstract provided.


Free Radicals Run In Lizard Families, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott, Mo Healey, Wanger Thomas Dec 2007

Free Radicals Run In Lizard Families, Mark Wilson, Mats Olsson, Tobias Uller, Caroline Isaksson, Beth Mott, Mo Healey, Wanger Thomas

Mark R Wilson

No abstract provided.


Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark Wilson, Karl A Bettelheim, X Y Wu, S Driesen, James Chin, Toni Chapman, I Barchia, D Trott Dec 2005

Comparison Of Virulence Gene Profiles Between Escherichia Coli Strains Isolated From Healthy And Diarrheic Swine, Mark Wilson, Karl A Bettelheim, X Y Wu, S Driesen, James Chin, Toni Chapman, I Barchia, D Trott

Mark R Wilson

No abstract provided.


Lymphotoxin-Beta Receptor-Dependent Genes In Lymph Node And Follicular Dendritic Cell Transcriptomes, Mark Wilson, C. Huber, C. Thielen, Y. Fu, E. Heinen, A Aguzzi, G Miele Dec 2004

Lymphotoxin-Beta Receptor-Dependent Genes In Lymph Node And Follicular Dendritic Cell Transcriptomes, Mark Wilson, C. Huber, C. Thielen, Y. Fu, E. Heinen, A Aguzzi, G Miele

Mark R Wilson

No abstract provided.


Detecting Mitochondrial Permeability Transition By Confocal Imaging Of Intact Cells Pinocytically Loaded With Calcein, Rachel Jones, Alison Smail, Mark Wilson Dec 2001

Detecting Mitochondrial Permeability Transition By Confocal Imaging Of Intact Cells Pinocytically Loaded With Calcein, Rachel Jones, Alison Smail, Mark Wilson

Mark R Wilson

When studied in vitro, mitochondrial permeability transition (MPT) is associated with an increase in mitochondrial permeability to solutes up to 1500 Da in mass and a loss of electrical potential difference across the inner mitochondrial membrane (delta psi mit). The MPT has been implicated as being important in cellular calcium homeostasis, autophagy and cell death via necrosis and apoptosis. Thus, it is important to develop a valid technique for accurate measurement of this phenomenon in intact cells. We developed a procedure for the detection of MPT in intact cells that avoids disadvantages associated with earlier approaches. In this new technique, …