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Full-Text Articles in Life Sciences
Ans Binding Reveals Common Features Of Cytotoxic Amyloid Species, Benedetta Bolognesi, Janet Kumita, Teresa Barros, Elin Esbjorner, Leila Luheshi, Damian Crowther, Mark Wilson, Christopher Dobson, Giorgio Favrin, Justin Yerbury
Ans Binding Reveals Common Features Of Cytotoxic Amyloid Species, Benedetta Bolognesi, Janet Kumita, Teresa Barros, Elin Esbjorner, Leila Luheshi, Damian Crowther, Mark Wilson, Christopher Dobson, Giorgio Favrin, Justin Yerbury
Mark R Wilson
Oligomeric assemblies formed from a variety of disease-associated peptides and proteins have been strongly associated with toxicity in many neurodegenerative conditions, such as Alzheimer's disease. The precise nature of the toxic agents, however, remains still to be established. We show that prefibrillar aggregates of E22G (arctic) variant of the A beta(1-42) peptide bind strongly to 1-anilinonaphthalene 8-sulfonate and that changes in this property correlate significantly with changes in its cytotoxicity. Moreover, we show that this phenomenon is common to other amyloid systems, such as wild-type A beta(1-42), the 159T variant of human lysozyme and an SH3 domain. These findings are …
Decarboxylation Of L-Dopa And 5-Hydroxytryptophan In Dispersed Rat Pancreas Acinar Cells, E. Yu, L. Stern, A. Tenenhouse
Decarboxylation Of L-Dopa And 5-Hydroxytryptophan In Dispersed Rat Pancreas Acinar Cells, E. Yu, L. Stern, A. Tenenhouse
Edward Yu
Amino acid decarboxylation activity in dispersed rat pancreas acinar cells and fractions derived by differential centrifugation of homogenate of these cells was studied. The rate of decarboxylation was measured by determining the rate of production of the [3H]-amine from [3H]-amino acid or the rate of production of 14CO2 from the [14C]-carboxy-labelled amino acid. Only the hydroxylated amino acids L-dopa and 5-hydroxytryptophan are decarboxylated by intact dispersed pancreas acinar cells or cell homogenates at all pH values and amino acid concentrations tested. The decarboxylase activity is located exclusively in the cell cytosol. Each substrate competitively inhibits the decarboxylation of the other …
Heat Shock Protein 70 Inhibits Alpha-Synuclein Fibril Formation Via Preferential Binding To Prefibrillar Species, Mark Wilson, M. M. Dedmon, J. Christodoulou, Christopher Dobson
Heat Shock Protein 70 Inhibits Alpha-Synuclein Fibril Formation Via Preferential Binding To Prefibrillar Species, Mark Wilson, M. M. Dedmon, J. Christodoulou, Christopher Dobson
Mark R Wilson
No abstract provided.