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2014

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Articles 301 - 306 of 306

Full-Text Articles in Life Sciences

Purification Of An Inducible Dnase From A Thermophilic Fungus, Kyle Landry, Andrea Vu, Robert Levin Dec 2013

Purification Of An Inducible Dnase From A Thermophilic Fungus, Kyle Landry, Andrea Vu, Robert Levin

Kyle S Landry

The ability to induce an extracellular DNase from a novel thermophilic fungus was studied and the DNAse purified using both traditional and innovative purification techniques. The isolate produced sterile hyphae under all attempted growing conditions, with an average diameter of 2 μm and was found to have an optimal temperature of 45 °C and a maximum of 65 °C. Sequencing of the internal transcribed region resulted in a 91% match with Chaetomium sp., suggesting a new species, but further clarification on this point is needed. The optimal temperature for DNase production was found to be 55 °C and was induced by the …

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Purification And Characterization Of Iso-Ribonucleases From A Novel Thermophilic Fungus, Kyle Landry, Robert Levin Dec 2013

Purification And Characterization Of Iso-Ribonucleases From A Novel Thermophilic Fungus, Kyle Landry, Robert Levin

Kyle S Landry

A thermophilic fungus previously isolated from composted horse manure was found to produce extracellular iso-RNases that were purified 127.6-fold using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of purification was determined electrophoretically using 4%–15% gradient polyacrylamide gels. RNase activity was dependent on the presence of a metal co-factor with significantly more activity with Zn2+ or Mn2+ than Mg2+. The RNases exhibited maximum activity at both pH 3.0 and pH 7.0 with no activity at pH 2.0 or 10.0. The optimal temperature for the iso-RNase was 70 °C. The …

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One Step At A Time: A Journey Of Hope, Inspiration, And Determination, Melinda Hermanns, Barbara K. Haas Dec 2013

One Step At A Time: A Journey Of Hope, Inspiration, And Determination, Melinda Hermanns, Barbara K. Haas

Melinda Hermanns

Approximately three million persons in the United States face the challenge of living with Parkinson's Disease (PD). In spite of medical and surgical interventions, the disease progresses, resulting in bradykinesia, rigidity and postural instability that impair functional ability and quality of life. The purpose of this case study is to illuminate the impact an exercise program may have on an individual with PD. A single case study design was used to explore the effect of exercise on the quality of life of a 73-year old gentleman with advanced PD. Interviews and field observations of the client, wife, and staff at …

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Ankle Plantarflexion Strength In Rearfoot And Forefoot Runners: A Novel Clusteranalytic Approach, Dominik Liebl, Steffen Willwacher, Joseph Hamill, Gert-Peter Brüggemann Dec 2013

Ankle Plantarflexion Strength In Rearfoot And Forefoot Runners: A Novel Clusteranalytic Approach, Dominik Liebl, Steffen Willwacher, Joseph Hamill, Gert-Peter Brüggemann

Joseph Hamill

The purpose of the present study was to test for differences in ankle plantarflexion strengths of habitually rearfoot and forefoot runners. In order to approach this issue, we revisit the problem of classifying different footfall patterns in human runners. A dataset of 119 subjects running shod and barefoot (speed 3.5 m/s) was analyzed. The footfall patterns were clustered by a novel statistical approach, which is motivated by advances in the statistical literature on functional data analysis. We explain the novel statistical approach in detail and compare it to the classically used strike index of Cavanagh and Lafortune (1980). The two …

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Effects Of Direct-Fed Microbial Supplementation On Broiler Performance, Intestinal Nutrient Transport And Integrity Under Experimental Conditions With Increased Microbial Challenge, G. R. Murugesan, N. K. Gabler, M. E. Persia Dec 2013

Effects Of Direct-Fed Microbial Supplementation On Broiler Performance, Intestinal Nutrient Transport And Integrity Under Experimental Conditions With Increased Microbial Challenge, G. R. Murugesan, N. K. Gabler, M. E. Persia

G. R. Murugesan

1. The effects of Aspergillus oryzae- and Bacillus subtilis- based direct-fed microbials (DFM) were investigated on the performance, ileal nutrient transport and intestinal integrity of broiler chickens, raised under experimental conditions, with increased intestinal microbial challenge. 2. The first study was a 3 × 2 factorial experiment, with 3 dietary treatments (control (CON), CON + DFM and CON + antibiotic growth promoter) with and without challenge. Chicks were fed experimental diets from 1 to 28 d, while the challenge was provided by vaccinating with 10 times the normal dose of commercial coccidial vaccine on d 9. In a second experiment, …

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Influence Of A Direct-Fedmicrobial And Xylanase Enzyme On The Dietary Energy Uptake Efficiency And Performance Of Broiler Chickens, G. R. Murugesan, M. E. Persia Dec 2013

Influence Of A Direct-Fedmicrobial And Xylanase Enzyme On The Dietary Energy Uptake Efficiency And Performance Of Broiler Chickens, G. R. Murugesan, M. E. Persia

G. R. Murugesan

BACKGROUND: Efficacy of a multi-strain direct-fed microbial product (PoultryStar® ME; PS) and a xylanase enzyme product on the dietary energy utilization efficiency and resulting performance in broiler chickens was evaluated. Apart from performance parameters, cecal and serum metabolites and activities of hepatic enzymes involved in energy metabolism were also determined. Ross 308 chicks were fed one of four experimental diets [control (CON), CON + PS, CON + xylanase and CON + PS + xylanase] using a 2×2 factorial arrangement from 1–21 days of age.

RESULTS: Cecal proportions of propionate and butyrate, as well as total short-chain fatty acid concentration were …

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