Life Sciences Commons^™

The Evidence For Pain In Fish: The Use Of Morphine As An Analgesic, Lynne U. Sneddon

Veterinary Science and Medicine Collection

This paper discusses the evidence for pain perception in fish and presents new data on morphine analgesia in fish. Recent anatomical and electrophysiological studies have demonstrated that fish are capable of nociception, the simple detection of a noxious, potentially painful stimulus and the reflex response to this. To prove pain perception, it must be demonstrated that an animal’s behaviour is adversely affected by a potentially painful event and this must not be a reflex response. The present study examined the acute effects of administering a noxious chemical to the lips of rainbow trout (Oncorhynchus mykiss) to assess what changes occurred …

Sheep Updates 2003 - Plenaries, Ashley Mercy, David Scobie, Neil Buchanan, Kevin Bell, John Milton, Graeme Martin, Bob Hall, Maurie Stephen, Rob Woodgate

Sheep Updates

This session covers eight papers from different authors:1. Biosecurity in the sheep industry will improve production and profits Ashley Mercy, Department of Agriculture, Western Australia 2. Easy care sheep David Scobie, AgResearch, PO Box 60 Lincoln, 8152, New Zealand 3. Targeting the market’s requirements - live sheep exports Neil Buchanan, Manager Animal Health Operations, Primary Industries & Resources, South Australia 4. Setting flock breeding objectives Kevin Bell, Sheep Management and Production Consultants, Kojonup WA 5. Future feeding of sheep in Western Australia John Milton and Graeme Martin, The University of Western Australia 6. Recipe for getting back into Sheep Bob …

Do Fishes Have Nociceptors? Evidence For The Evolution Of A Vertebrate Sensory System, Lynne U. Sneddon, Victoria A. Braithwaite, Michael J. Gentle

Veterinary Science and Medicine Collection

Nociception is the detection of a noxious tissue-damaging stimulus and is sometimes accompanied by a reflex response such as withdrawal. Pain perception, as distinct from nociception, has been demonstrated in birds and mammals but has not been systematically studied in lower vertebrates. We assessed whether a fish possessed cutaneous nociceptors capable of detecting noxious stimuli and whether its behavior was sufficiently adversely affected by the administration of a noxious stimulus. Electrophysiological recordings from trigeminal nerves identified polymodal nociceptors on the head of the trout with physiological properties similar to those described in higher vertebrates. These receptors responded to mechanical pressure, …

Multiple Luteinizing Hormone Receptor (Lhr) Protein Variants, Interspecies Reactivity Of Anti-Lhr Mab Clone 3b5, Subcellular Localization Of Lhr In Human Placenta, Pelvic Floor And Brain, And Possible Role For Lhr In The Development Of Abnormal Pregnancy, Pelvic Floor Disorders And Alzheimer's Disease, A Bukovsky, K Indrapichate, H Fujiwara, Maria Cekanova Ms, Rndr, Phd, Me Ayala, R Dominguez, Mr Caudle, J Wimalsena, Rf Elder, P Copas, Jf Foster, Ri Fernando, Dc Henley, Nb Upadhyaya

Maria Cekanova MS, RNDr, PhD

Distinct luteinizing hormone receptor (LHR) protein variants exist due to the posttranslational modifications. Besides ovaries, LHR immunoreactivity (LHRI) was also found in other tissues, such as the brain, fallopian tube, endometrium, trophoblast and resident tissue macrophages. The 3B5 mouse monoclonal antibody was raised against purified rat LHR. In rat, porcine and human ovaries, the 3B5 identified six distinct LHR bands migrating at approximately 92, 80, 68, 59, 52 and 48 kDa. Characteristic LHRI was detected in rat, human and porcine corpora lutea. During cellular differentiation, subcellular LHR distribution changed from none to granular cytoplasmic, perinuclear, surface, nuclear and no staining. …

Trigeminal Somatosensory Innervation Of The Head Of A Teleost Fish With Particular Reference To Nociception, Lynne U. Sneddon

Veterinary Science and Medicine Collection

Trigeminal somatosensory receptors have not been characterised in teleost fish and studies in elasmobranchs have failed to identify nociceptors. The present study examined the trigeminal nerve of a teleost fish, the rainbow trout (Oncorhynchus mykiss) to determine what types of somatosensory receptors were present on the head of the trout specifically searching for nociceptors. Single unit recordings were made from receptive fields on the head of the fish innervated by the trigeminal nerve. Each receptive field was tested for sensitivity to mechanical, thermal and chemical stimulation. Five different receptor types were found: fast adapting receptors responding to mechanical stimulation; slowly …

Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady

Honors Theses

No abstract provided.

Addressing The Decline In Reproductive Performance Of Lactating Dairy Cows: A Researcher’S Perspective, William J. Silvia

Animal and Food Sciences Faculty Publications

No abstract provided.

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Maria Cekanova MS, RNDr, PhD

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Osmosensitive Taurine Release: Does Taurine Share The Same Efflux Pathway With Chloride And Other Amino Acid Osmolytes?, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Swelling subsequent to hyposmotic conditions activates a process of volume regulation present in most cell types. This volume adjustment is accomplished by osmolyte translocation toward the extracellular space to reach a new osmotic equilibrium. Molecules involved in this homeostatic mechanism have been broadly classified into two categories: organic and inorganic osmolytes. Inorganic osmolytes comprise mainly the intracellular ions K⁺ and Cl^–. Cell swelling–induced activation of separate K⁺ and Cl^– channels has been described in most preparations. Organic osmolytes are grouped in three categories: amino acids, polyalcohols, and methylamines. These osmolytes, particularly taurine, are present in …

Mucosal Priming Of Simian Immunodeficiency Virus-Specific Cytotoxic T-Lymphocyte Responses In Rhesus Macaques By The Salmonella Type Iii Secretion Antigen Delivery System, David T. Evans, Li-Mei Chen, Jacqueline Gillis, Kuei-Chin Lin, Brian Harty, Gail P. Mazzara, Ruben O. Donis, Keith G. Mansfield, Jeffrey D. Lifson, Ronald C. Desrosiers, Jorge E. Galán, R. Paul Johnson

School of Veterinary and Biomedical Sciences: Faculty Publications

Nearly all human immunodeficiency virus (HIV) infections are acquired mucosally, and the gut-associated lymphoid tissues are important sites for early virus replication. Thus, vaccine strategies designed to prime virus-specific cytotoxic T lymphocyte (CTL) responses that home to mucosal compartments may be particularly effective at preventing or containing HIV infection. The Salmonella type III secretion system has been shown to be an effective approach for stimulating mucosal CTL responses in mice. We therefore tested ∆phoP-phoQ attenuated strains of Salmonella enterica serovar Typhimurium and S. enterica serovar Typhi expressing fragments of the simian immunodeficiency virus (SIV) Gag protein fused to the …

Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention provides a method to detect the presence of a microorganism or agent in an animal. The method encompasses placement of devices at various locations where the animal resides so as to induce the animal to initiate contact with the device. As a result of this contact, the animal deposits various microorganisms and agents on the device. The device is then tested for the presence of the particular microorganism or agent of interest.

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Volume 1, Number 3 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Booming Biotechnology
• Lessons in Leadership
• New Options for Producers
• Entering an Exciting New Era

Volume 1, Number 2 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Insects as Teaching Tools
• Helping Children Succeed
• Honors Student Faces Bright Future
• Reinventing the Family Farm

Volume 1, Number 1 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Adding Value to Dogwoods
• A Building to Advance Science
• Teaching Lessons in Life
• Towards a Brighter Forest Future

Characterization Of A Piscirickettsiosis-Like Disease In Hawaiian Tilapia., M. J. Mauel, Debra L. Miller, K. Frazier, A. Liggett, E. Styer, D. Montgomery-Brock, J. Brock

Debra L Miller

In 1994, tilapia (Oreochromis mossambicus and Sarotherodon melanotheron) in wild and farmed populations on Oahu, Hawaii, USA, began to die of an unknown disease that was similar but not identical to piscirickettsiosis in salmonids. Only tilapia were affected. Diseased tilapia often swam erratically and had trouble staying at depth. Scattered cutaneous haemorrhage and exophthalmia were often noted. In many cases, fish were found dead with no clinical signs. Gills exhibited epithelial hyperplasia with severe multifocal consolidation of secondary lamellae. Multiple granulomas were observed in the gills, spleen, kidney, choroid gland and testes, but not in the liver. Tilapia mortalities occurred …

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Maria Cekanova MS, RNDr, PhD

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Isolation Of Klebsiella Pneumoniae From Mares With Metritis And Stallions, Detection Of Biotypes And Capsule Types, N. Yakut Özgür, Arzu Funda Bağcigi̇l, Serkan İki̇z, M. Ragip Kiliçarslan, Babür Cari̇oğlu, Ati̇lla Ilgaz

Turkish Journal of Veterinary & Animal Sciences

In 2000-2001, the frequency of metritis cases due to Klebsiella pneumoniae was investigated in Thoroughbred breeding mares in the Marmara region of Turkey, where most racehorses are reared. In addition, Thoroughbred stallions from the breeding centre in the İzmit district were examined for the presence of K. pneumoniae. Biotypes and capsule types of the isolates were detected, and their antibiotic susceptibilities were determined. Intrauterine swabs taken from 56 mares with metritis, and semen samples and swabs of urethral fossa from 27 stallions were investigated. K. pneumoniae was isolated from 13 (23.2%) mares and 2 (7.4%) stallions. Biotyping of the isolates …

Isovolumetric Regulation In Mammal Cells: Role Of Taurine, B. Ordaz, R. Franco, National University Of Mexico Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

The ability to regulate cell volume is an ancient conserved trait present in essentially all species through evolution. The maintenance of a constant cell volume is a homeostatic imperative in animal cells. Changes in cell water content affecting the concentration of intracellular messenger molecules impair the complex signaling network, crucial for cell functioning and intercellular communication. Although the renal homeostatic mechanisms exert a precise control of extracellular fluid osmolarity, this is challenged in a variety of pathological situations. The intracellular volume constancy is continuously compromised by the generation of local and transient osmotic microgradients, associated with nutrients uptake, secretion, cytoskeleton …

The Effects Of Some Silage Additives In Sorghum Silage On The Silage Quality And Ruminal Degradabilities Of Nutrients Ii- Ruminal Degradabilities Of Nutrients, N. Tuğba Bi̇ngöl, Erol Baytok

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine the effects of different silage additives on the ruminal degradability of sorghum silage. Different sorghum silages were prepared from sorghum harvested at the milk or dough stage (287.40 and 304.80 g/kg DM) with or without SIL-ALL (enzyme-inoculant complex; EI), molasses (M), formic acid (FA), M + FA, EI + FA, EI + M and EI + FA + M additives in laboratory silos (glass jar 1 kg in volume) and incubated for two months. After incubation, the silos were opened and dry matter and ADF analyses were done. The dry matter and …

Immunohistochemical Detection Of Heparin-Binding Lectin In The Development Of The Bovine Thymus, Kami̇l Seyrek, Ayşegül Bi̇ldi̇k

Turkish Journal of Veterinary & Animal Sciences

Heparin is a heterogeneous mixture of highly charged glycosaminoglycans that can exert a variety of biological and biochemical effects. Increasing attention is being paid to its role in cellular processes such as angiogenesis, cellular attachment, growth modulation and smooth muscle cell proliferation. The characterisation of cellular receptors for heparin-binding proteins is a rational step in the quest to elucidate the biochemical basis of specific binding and heparin-mediated responses. We studied the distribution of heparin-binding lectin at the optical level during bovine thymus development on sections of fixed tissue using antibodies raised against this lectin. The presence of ligands accessible to …

A Study On The Effectiveness Of Breeding And Support Policies In Turkey's Cattle Sector, Fahri̇ Yavuz, Ömer Akbulut, Ati̇lla Keski̇n

Turkish Journal of Veterinary & Animal Sciences

The proportion of Turkey's cattle that were from high-gene cattle breeds and their crosses has increased from 0.8% to 60.7% over the last four decades. While the total number of cattle declined by 1.65 million head, total milk and meat production increased by 298% and 428% to 8.91 million and 359,000 tons, respectively. At the same time, milk per cow and meat per head increased by 184% and 144% to 1688 kg and 171 kg, respectively. According to a three-stage least-square regression analysis, milk per cow and meat per head, support policies since 1972 and milk and meat prices had …

The Effect Of Different Enzyme Supplementations On The Performance Of Laying Hens Fed With Diets Based On Barley, M. Aki̇f Yörük, Duran Bolat

Turkish Journal of Veterinary & Animal Sciences

The effects of replacing corn in the diets of laying hens with 100% barley containing different enzymes as an energy source on different yields and egg quality traits were investigated in this study. A total of 300 laying hens (average 22 weeks of age) were used. The study was carried out with five groups, 2 controls (60% corn, 60% barley) and 3 experimental groups. Each group of 60 hens was also divided into six subgroups. Enzymes such as Betaglucanase (Allzyme BG), xylanase (Allzyme PT) and amylase (Allzyme AB) were separately added at a rate of 0.05% in the order of …

Immunoperoxidase Studies On Leptospirosis In Bovine Abortion, Ahmet Temur, Yavuz Seli̇m Sağlam

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine leptospirosis from aborted bovine fetuses. Tissue sections of the liver, lung, spleen and kidney were stained with avidin-biotin peroxidase complex, and antigen localization was examined. These examinations showed positive staining in eight (24.24%) out of 33 fetuses for the presence of leptospira antigens. In these eight cases, leptospira antigens were found in three lung (37.5%), two liver (25%), four kidney (50%) and four spleen (50%) samples. Microscopic studies demonstrated that leptospiral antigens were located in the interalveolar and interlobular septum of the lung; in the cytoplasm of hepatocytes and macrophages in the portal regions …

Growth Performance And Food Conversion Ratio Of Siberian Sturgeon (Acipenser Baeri Brandt) At Different Daily Feeding Rates, Feri̇t Rad, Gülten Köksal, Mevlüt Kindir

Turkish Journal of Veterinary & Animal Sciences

The effects of different daily feeding rates on the specific growth rate and food conversion ratio of Siberian sturgeon (Acipenser baeri) were investigated in order to determine the optimum daily feeding rate for fish with an initial mean weight of 1736 ± 37 g. To this end, a Latin Square experimental set-up was designed consisting of four groups of 25 fish fed a rainbow trout diet at four different daily feeding rates (0.75, 1.00, 1.25 and 1.50% body weight/day) for four 2-week periods. Water temperature varied between 19 and 22 °C throughout the experiment. Mean specific growth rate and food …

The Effects Of Some Silage Additives In Sorghum Silage On The Silage Quality And Ruminal Degradabilities Of Nutrients I. The Effects On Silage Quality, N. Tuğba Bi̇ngöl, Erol Baytok

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine the effects of different silage additives on the silage quality of sorghum silage. Different sorghum silages were prepared from sorghum harvested at the milk or dough stage (287.40 and 304.80 g/kg DM) with or without SIL-ALL (enzyme-inoculant complex; EI), molasses (M), formic acid (FA), M + FA, EI + FA, EI + M and EI + FA + M additives in laboratory silos (glass jar 1 kg in volume) and incubated for two months. After incubation, the silos were opened and chemical and physical analyses were done. The dry matter contents of …

Development Of An Enzyme Immunoassay For The Determination Of Testosterone, Bülent Güven, Semi̇n Özsar, Nalan Maraşli, Şaban Maraşli, Ayla Özcan

Turkish Journal of Veterinary & Animal Sciences

In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be

Incubation And Yolk Sac Periods Of Closely Purebred And Reciprocal Hybrids Of Erzurum And Silifke Rainbow Trout (Oncorhynchus Mykiss), N. Mevlüt Aras, Özer Ayik, E. Mahmut Kocaman, Telat Yanik

Turkish Journal of Veterinary & Animal Sciences

Our aim was to investigate the survival rate at the hatching and yolk sac stage of close-rearing and crossbred rainbow trout population (Erzurum female X Erzurum male - E_F x E_M; Erzurum female X Silifke male - E_F x S_M; Silifke female X Silifke male - S_F x S_M; Silifke female X Erzurum male - S_F x E_M). A total of 32,976 eggs were incubated, 29,012 of which hatched. The survival rate of the larvae was 88%. The insemination rate, brooding yield and hatching rate of E_F x E_M, E_F x S_M, S_F x S_M and S_F x E_M were …

Determination Of The Allele Frequency Of Some Genomic Loci In A Holstein Cattle Population And Its Importance In Individual Identification, Harun Ceri̇t

Turkish Journal of Veterinary & Animal Sciences

Nowadays, the use of DNA sequences with genetic techniques and DNA polymorphism as genetic markers is increasing very rapidly. In this study, we examined the frequencies of seven different polymorphic loci in the cattle population of İstanbul University Veterinary Medicine Research and Application Farm. Thus, our aim was to examine and calculate the power of exclusion, match probability and power of discrimination of the seven loci, and examine the usefulness of the involved loci in identification tests. For DNA extraction, Chelex and Phenol-Chloroform-Isoamil alcohol methods were applied to the blood taken from animals. After measuring the amount and purity degree …