Life Sciences Commons^™

International Shipping Of Fumonisins From Maize Extracts On C18 Sorbent, R. T. Riley, O. A. Torres, E. Palencia

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/ inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to conduct a survey of fumonisins in Central America. The objective was to develop a method for isolating and shipping maize extracts for fumonisin analysis so as to facilitate a survey of fumonisin exposure. The results indicate that fumonisins …

Why Is Maize A Sacred Plant? Social History And Agrarian Change On Sumba, Cynthia Twyford Fowler

Faculty Scholarship

Why has maize, a plant with origins in the New World, become ritually important in an indigenous Southeast Asian religion? While environmental conditions and agricultural economics are key determinants of everyday resource management practices in insular Southeast Asia, it is necessary to consider ethnic identity, political economy, and social structure in order to understand the religious significance of natural resources in contemporary society. Linguistic, cosmological, and horticultural data are combined with an analysis of local perceptions of culture and environment. This information is used to explain the transformation of an introduced plant into an indigenous sacrament. Ethnographic data, including a …

Effects Of Cowpea Fortification, Dehydration Method And Storage Time On Some Quality Characteristics Of Maize-Based Traditional Weaning Foods., Emmanuel Ohene Afoakwa, Samuel Sefa-Dedeh, Esther Sakyi-Dawson

Professor Emmanuel Ohene Afoakwa

Fortification of cereal-based traditional foods with legume protein can improve their nutritional value. It is, however, important to find out the extent to which the addition of cowpea affects the desirable quality characteristics of traditional weaning foods prepared from fermented maize and also to assess the effect of dehydration method and storage time on the chemical, physico-chemical and functional properties of the products. A 3 x 2 x 4 factorial experiment with cowpea level, drying method and storage time as the respective variables was done. The traditional weaning food was prepared by steeping maize in water for 24 hours, mixed …

Feasibility Of Site-Specific Management Of Corn Hybrids And Plant Densities In The Great Plains, J.F. Shanahan, Thomas A. Doerge, Jerry J. Johnson, Merle F. Vigil

Department of Agronomy and Horticulture: Faculty Publications

The goal of this research was to determine the potential for use of site-specific management of corn hybrids and plant densities in dryland landscapes of the Great Plains by determining (1) within-field yield variation, (2) yield response of different hybrids and plant densities to variability, and (3) landscape attributes associated with yield variation. This work was conducted on three adjacent fields in eastern Colorado during the 1997, -98, and -99 seasons. Treatments consisted of a combination of two hybrids (early and late maturity) and four plant densities (24,692, 37,037, 49,382 and 61,727 plants ha^-1) seeded in replicated long …

Iaa Breakdown And Its Effect On Auxin-Induced Cell Wall Acidification In Maize Coleoptile Segments, Winfried Peters, Christa Lommel, Hubert Felle

Winfried S. Peters

Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen is essential for crop production, but also contributes to eutrophication of surface water and degradation of drinking water quality. Modern corn production requires relatively large quantities of N, which are generally supplied by fertilizers. Over-application of N fertilizers and animal wastes frequently results in nitrate leaching. Synchronizing N availability with crop N need offers the potential to protect the environment without sacrificing production. Tools are needed to rapidly and easily monitor crop N status to make timely decisions regarding fertilizer application. Analytical and optical techniques were evaluated with greenhouse grown corn at silking to evaluate several methods to monitor …

Genetic Dissection Of The Morphological Evolution Of Maize, John Doebley

Aliso: A Journal of Systematic and Floristic Botany

Maize (Zea mays ssp. mays) and its wild progenitor, teosinte (Z. mays ssp. parviglumis) differ dramatically in inflorescence and plant architecture despite the fact that their evolutionary divergence occurred within the past 10,000 years or less. To elucidate the genetic control of the morphological differences between maize and teosinte, my colleague and I employed quantitative trait locus mapping with molecular markers. Results indicated that most of the variation in plant and inflorescence morphology between maize and teosinte can be explained by five restricted regions of the genome. In this paper, characterization of three of these regions …

Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …

Molecular-Marker-Facilitated Investigations Of Quantitative-Trait Loci In Maize. I. Numbers, Genomic Distribution And Types Of Gene Action, Jonathan F. Wendel, M. D. Edwards, Charles W. Stuber

Jonathan F. Wendel

Individual genetic factors which underlie variation in quantitative traits of maize were investigated in each of two F 2 populations by examining the mean trait expressions of genotypic classes at each of 17-20 segregating marker loci. It was demonstrated that the trait expression of marker locus classes could be interpreted in terms of genetic behavior at linked quantitative trait loci (QTLs). For each of 82 traits evaluated, QTLs were detected and located to genomic sites. The numbers of detected factors varied according to trait, with the average trait significantly influenced by almost two-thirds of the marked genomic sites. Most of …

Additional Mapping Of Isozyme Loci: Localization Of Acp4, Dia2, Adkl, Tpi3, And Sadl, Jonathan F. Wendel, Major M. Goodman, Charles W. Stuber

Jonathan F. Wendel

We recently listed the isozyme loci being studied by starch gel electrophoresis in our laboratory and summarized the available mapping data in a series of reports (Wendel et al., MGCNL 59:87-90). Subsequent work has resulted in further clarification of the chromosomal locations of markers on chromosomes 1 (Acp4, Dia2), 6 (Adkl), 8 (Tpi3), and 10 (Sadl). Previously unreported information on these loci and their chromosomal locations follows.

Tpi4 Is Located Near The Centromere On The Long Arm Of Chromosome 3, Jonathan F. Wendel, J. B. Beckett

Jonathan F. Wendel

Tpi4, one of the three genes encoding cytosolic triose phosphate isomerase isozymes, was earlier shown to be on 3L between Pgd2 (phosphogluconate dehydrogenase) and the centromere (Wendel et al., MNL 59:88). In an effort to better localize Tpi4, a series of crosses was made between Tpi4 testers and stocks carrying three B-A translocations believed to be near the centromere on 3L.

Localization Of Two New Isozyme Loci, Hexl And Tpi4, To Chromosome 3, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Recent work with starch gel electrophoresis of coleoptile extracts has indicated that Hexl, the structural locus for the most anodal set of hexokinase isozymes, and Tpi4, which encodes the slowest migrating set of triose phosphate isomerase bands, are located on chromosome 3.

Mapping Data For 34 Isozyme Loci Currently Being Studied, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

With the recent localization of several new loci, a composite listing of these loci and chromosomal locations has been compiled and is shown in Table 1. Although locations of some of the loci are tentative, many are very precisely located from studies involving 1600 to more than 1900 F2 plants.

Linkage Relationships Between A New Locus, Hex2, And Previously Assigned Loci On Chromosome 6, Jonathan F. Wendel, Charles W. Stuber, Major M. Goodman

Jonathan F. Wendel

Starch gel electrophoresis of maize coleoptile extracts in our laboratory has shown two zones of staining for isozymes of hexokinase. Genetic analyses 9 indicate that the slower migrating set of bands is encoded by the structural gene Hex2 and that the enzyme is monomeric. Recent linkage analyses and work with B-A translocation stocks (generously supplied by Jack Beckett) have allowed for the placement ofHex2 on the long arm of chromosome 6. Its location relative to other markers on 6L was determined from the analysis of two very large F2 populations generated for other reasons.

Growth Analysis Based On Degree Days, M. P. Russelle, Wallace Wilhelm, R. A. Olson, James F. Power

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Comparisons of growth analysis functions within and among experiments are often confounded by sources of variation other than those imposed by treatment. we suggest use of a temperature index, such as modified growing degree days, as the divisor in growth functions to facilitate treatment comparisons within certain experiments and to reduce the effects of differing temperature regimes among experiments on these comparisons. Three experiments were identified to provide data to analyze this new approach. Mean absolute growth rate (GR) and mean relative growth rate (RGR) were compared in two experiments with maize (Zea mays L.) conducted in eastern Nebraska. …

Temperature Effect On Leaf Water Potential Measurements, Rex J. Prosser

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Silver foil psychrometers were used to monitor water potential in different portions of maize leaves which were subjected to temperature gradients. The psychrometers were preliminarily calibrated over standard NaCI solutions at temperatures ranging from 5°C to 45°C in an aluminum plate apparatus designed to provide uniform temperatures within each psychrometer even while overall temperature was changing. Based on the calibrations, empirical temperature correction equations were derived and used to correct psychrometer readings at different temperatures to equivalent values at 25°C. Temperatures on a single maize leaf were varied by placing the long leaf through an insulated box with several compartments …

The Effects Of Irradiating Dormant Maize Seeds With Xrays And Thermal Neutrons, Rosalind Morris, E. F. Frolik

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

In 1951 a research program was started at the University of Nebraska to compare the developmental effects of thermal neutrons and X rays on different crop seeds. Three crops, barley, tomato and maize, were chosen for additional information involving induced chromosomal aberrations and seedling mutations. The maize investigations are presented in this bulletin, along with a comparison among the three crops with respect to irradiation effects.

Progress From Recurrent Selection Procedures For The Improvement Of Corn Populations, John H. Lonnquist

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The possibilities of developing synthetic varieties suitable for the fringe areas of the corn belt, where cost of hybrid seed is high relative to the value of the expected crop, and in areas where hybrid corn might not be a feasible approach for other reasons, made it seem a worthwhile objective for study. Effort has been made in recent years to increase the productivity of hybrid combinations through selection of new superior lines using standard breeding procedures. The limited progress realized has resulted in an increased interest in the possible use of alternate methods for the development of superior germplasm …

The Effects Of Thermal-Neutron Irradiation Of Maize And Barley Kernels, John W. Schmidt, E. F. Frolik

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

It is the purpose of this paper to report the effects of thermal-neutron irradiation of maize and barley kernels on subsequent germination, plant establishment, and early plant development. Comparisons are made with equivalent neutron treatments of maize pollen, results of which have been reported in part in previous publications.

The Inheritance Of A Recurring Somatic Variation In Variegated Ears Of Maize, R. A. Emerson

Historical Research Bulletins of the Nebraska Agricultural Experiment Station

The inheritance of variegation has special interest and importance in genetics. In this paper I shall present data from maize and attempt to show how they can be interpreted in strictly Mendelian terms.