Life Sciences Commons^™

Rice Blast Disease In The U.S. And Africa: Determination Of Pathogen Diversity And The Identification Of Resistance Genes For Disease Management, Felix Rotich

Graduate Theses and Dissertations

Rice blast caused by Magnaporthe oryzae (= Pyricularia oryzae) B. Couch, is a leading disease of rice. Magnaporthe oryzae exhibits a high degree of diversity. The diversity of isolates of M. oryzae from Africa and the U.S. were examined using vegetative compatibility and virulence phenotyping as well as determination of variation in the avirulence gene AVRPiz-t in isolates from Africa. Also, evaluation of blast resistance genes in the interspecific rice germplasm “New Rice for Africa” NERICA was done using F2 progeny of the cross of U.S. susceptible cultivar M204 and NERICA 12. The U.S. isolates were in three vegetative compatibility …

The Role Of G-Protein Signaling In Pathogenesis In Cercospora Zeae-Maydis, Brant Smith

Graduate Theses and Dissertations

Gray leaf spot, caused by Cercospora zeae-maydis, is one of the most destructive foliar diseases of maize worldwide. C. zeae-maydis orients hyphal growth towards stomata (stomatal tropism) and forms infectious structures (appressoria) that are necessary for successful infection. Although some genes involved in pathogenesis in C. zeae-maydis have been identified, the molecular mechanisms are not well understood. In fungi, heterotrimeric G-proteins consist of three subunits (α, β, and γ) and mediate responses to environmental stimuli. They regulate diverse functions, including nutrient detection, virulence, fungal development, conidiation, secondary metabolism, and pathogenesis in many plant pathogenic fungi. This research explored the role …

Storage Of Round And Square Switchgrass Bales: Effect Of Storage Time And Fungal Inoculation On Saccharification Efficiency, Noaa Frederick

Graduate Theses and Dissertations

To produce fermentable sugars from lignocellulosic biomass feedstock, severe pretreatment conditions are needed (either high acid concentration, temperature, or retention times). High severities can produce toxic byproducts which inhibit enzymatic hydrolysis or fermentation. In order to reduce pretreatment severities (and thus increase enzyme and fermentation efficiency), the white-rot fungus Pleurotus ostreastus was seeded into square and round bales of Kanlow switchgrass (Panicum virgastum L.) and left in the field over a period of 9 month. The laccase producing fungus is believed to selectively degrade lignin, a common plant structural polymer, which can function as an enzymatic inhibitor. Samples were taken …

Optimizing A Luciferase-Based Tool For Studying The Effects Of Fatty Acid Desaturase 7 On Singlet Oxygen Accumulation In Arabidopsis Thaliana, Abeer Muhammedali Alnasrawi

Graduate Theses and Dissertations

In plants, reactive oxygen species (ROS) are generated as a byproduct of normal metabolism, as well as in response to adverse conditions such as light stress, extreme temperatures, and exposure to pests and pathogens. Singlet oxygen (1O2) is a ROS that is formed during photosynthesis in photosystem II (PSII) of the chloroplasts. Levels of 1O2 and other ROS are tightly controlled in healthy plants, but some studies suggest that levels of fatty acid desaturase (FAD) activity in Arabidopsis thaliana can influence constitutive and/or stress-responsive ROS accumulation. In this study, a luciferase-based reporter gene that is selectively stimulated by 1O2 (AAA-ATPase: …

Stink Bugs: Spatial Distribution, Pecan Phenological Susceptibility And Sampling Program, Brian Cowell

Graduate Theses and Dissertations

An effective management program for stink bugs (SBs) in pecan groves requires knowledge of: stages of the pecans susceptible to SB damage; strata of the tree with SB damaged nuts; a practical SB monitoring method; and, effects of landscapes contributing SBs into pecan groves.

Stink bugs produced feeding punctures in pecan shucks at all phenological stages. Pecans punctured before the dough stage drop from the tree. Kernel damage occurs in the pecans’ dough stage, whereas mature pecans are not damaged.

The pecans collected from the whole tree using the tree shaker had significantly less punctures than the pecans collected from …

Field Screening Of Diverse Soybean Cultivars For Flood Tolerance, Jane Achando Khatenje Mokua

Graduate Theses and Dissertations

Flooding can significantly reduce soybean growth, development, and yield. Therefore, screening and identification of flood-tolerant soybeans will enhance development of cultivars that are well suited for flood-prone areas. Sets of screening tests were conducted in Stuttgart, Arkansas using three separate but related experiments, with the purpose of establishing effective flood tolerance screening protocol that can identify tolerant and sensitive cultivars. Each experiment was completely randomized with three replications. The first experiment involved screening of 256 maturity group (IV and V) cultivars. Flooding was imposed at the fifth-node (V5) or first-bloom (R1) stage for ten days. Post-flood visual ratings and stand …

Understanding The Causal Agent Of Rose Rosette Disease, Patrick Louis Di Bello

Graduate Theses and Dissertations

A number viruses are known to infect roses, ranging from those in the genera Nepovirus, and Ilarvirus, which have been reported since the inception of rose virology, to recently discovered viruses in the genera Carmovirus, Closterovirus, Emaravirus, Luteovirus, Rosadnavirus, and Potyvirus. Of the viral diseases in rose, arguably the most damaging is Rose rosette (RRD), which is associated with the Emaravirus, Rose rosette virus (RRV). The objective of this thesis is to fill in the gaps in knowledge on the epidemiological aspects of RRD and RRV. There has been significant progress in the epidemiology of the RRD agent prior to …

Analysis Of Ac/Ds Activation Tagged Mutants In Tomato (Solanum Lycopersicum), Ipeleng Randome

Graduate Theses and Dissertations

Tomato (Solanum lycopersicum) is a crop of immense economic and nutritional importance worldwide and also a good model organism for genomic studies of other dicot species. The recent completion of the tomato genome sequence is a great milestone towards learning about the tomato genome. Elucidation of the function of the different genes using different functional genomic tools is therefore important in adding to this resource. To this end, we have developed an Ac-Ds transposon ‘activation tagging’ (ATag) system to be able to transpose transposon inserts, bearing a strong 35S-enhancer element, all around the genome. An Ac-Ds ATag construct was used …

Utilization Of Weed Ecology, Tank Mixtures, And Application Technology To Improve Efficacy Of Herbicide Applications In Soybean, Christopher John Meyer

Graduate Theses and Dissertations

New herbicide-resistant soybean traits will increase the number of herbicides that can be applied in soybean and change the technology currently used to make herbicide applications in soybean. Palmer amaranth (Amaranthus palmeri) remains one of the most difficult weeds to manage in soybean and thus is the primary focus of this research. Therefore, experiments were conducted to investigate Palmer amaranth temporal emergence, control of common Arkansas weeds with new soybean herbicide programs, and the effect of application technology on dicamba-containing tank mixtures. Palmer amaranth emergence exceeded 400 plants m-2 for tillage treatments in 2013 and 2014, and tillage events typically …

Use Of Hppd-Inhibiting Herbicides For Control Of Common Weeds In Arkansas And Current Status Of Herbicide-Resistance Among Echinochloa Populations In Arkansas, Clay Starkey

Graduate Theses and Dissertations

Herbicide-resistant weeds in Arkansas cause problems for growers. Up-to-date information and new technologies can help plan mitigation strategies to slow resistant weeds. The objectives of this research were to provide a ‘snapshot’ of herbicide-resistant Echinochloa spp. in rice producing counties, determine how much resistance has spread across the state, and understand the effectiveness of 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides for control of glyphosate-resistant (GR) Palmer amaranth relative to commercial standards currently labeled in soybean. To assess the prevalence of Echinochloa spp. resistance, 82 samples were collected from 23 rice producing counties in 2010. The samples were tested for resistance to commonly …

Available Nitrogen And Denitrification In Soil Altered By Ground Cover And Nutrient Source In An Organic Apple Orchard, Jade Nicole Ford

Graduate Theses and Dissertations

A shift in public demand towards more organic and locally produced fruit and vegetables has been occurring across the United States in recent years. A common practice in organic fruit production is the application of organic ground covers to supply nutrients while enhancing other soil properties. A need for research exists in the southern region of the U.S. examining the effects of regionally applicable ground cover and nutrient management on nitrogen availability and the microbial community to provide information to organic farmers in the region. Two studies were conducted to determine how 12 treatment combinations of four ground covers (compost, …

Cotton Injury Due To Soil- Or Foliar-Applied Herbicides: An Assessment Based On The Influences Of Genetic, Agronomic, And Environmental Factors, Brandon William Schrage

Graduate Theses and Dissertations

Increasing populations of glyphosate-resistant weeds, such as Palmer amaranth, have prompted growers to pursue alternative means of weed control in cotton. In many cropping systems, this means the utilization of older chemistries and residual herbicides. The goal of this research was to evaluate and understand the agronomic and environmental factors that affect the inconsistent injury often associated with these herbicides as well as determine the impact of Palmer amaranth emergence date on seed production, biomass, and cotton yield. Experiments were conducted in three counties in Arkansas giving a distinct range of climate and soil texture. Injury, biomass, and number of …

Defining The Potassium Nutritional Requirements And Distribution Among Plant Parts Of Representative Soybean Cultivars From Different Maturity Groups, Md. Rasel Parvej

Graduate Theses and Dissertations

The potassium (K) requirement of soybean [Glycine max (L.) Merr.] was investigated to determine whether cultivar sensitivity to K deficiency was affected by growth habit (determinate or indeterminate) and how cultivars from each growth habit accumulate and distribute K among plant structures. We also diagnosed K deficiency across reproductive growth stages (R2-6) using trifoliolate leaf- and petiole-K concentrations and at harvest (R8) using seed-K concentration. Soybean responded similarly to K deficiency in terms of yield, selected yield components, and seed-K concentration, regardless of growth habit. The yield loss from K deficiency was greatest on the middle to upper nodes of …

Applying Molecular And Phenotypic Tools To Characterize Flesh Texture And Acidity Traits In The Arkansas Peach Breeding Program And Understanding The Crispy Texture In The Arkansas Blackberry Breeding Program, Alejandra Andrea Salgado Rojas

Graduate Theses and Dissertations

Quantitative trait loci (QTL) utilizing a pedigree-based analysis (PBA) approach was performed in the peach breeding program for the first time. The pedigree consisted of seven-F1 populations, their parents, ancestors, and cultivars. Flesh firmness and titratable acidity (TA) were examined in 2011, 2012, and 2013. For TA, a consistent and strong QTL was identified on the proximal end of linkage group (LG) 5 of the peach genome. For flesh firmness, two QTLs were located on LG 4. The first QTL was located on the chromosomal region where the slow-melting flesh (SMF) DNA test was identified, and the second QTL was …

Validation Of Soil-Test-Based Phosphorus And Potassium Fertilizer Recommendations For Rice And Soybean, Matthew Scott Fryer

Graduate Theses and Dissertations

The science of soil-testing for nutrient management and fertilizer recommendations is widely accepted among scientists and agronomists. Although this science is unsurpassed in predicting soil nutrient availability, soil-test interpretations are seldom validated. Major research objectives for irrigated soybean [Glycine max (L.) Merr.] and direct-seeded, delayed-flood rice (Oryza sativa L.) were to: i) validate the accuracy of Mehlich-3 soil-test P (STP) and K (STK) interpretations and ii) published critical tissue-P and -K interpretations in predicting the yield response to fertilizer at different significance levels (p≤0.05 to 0.25), iii) examine how seed nutrient concentrations are influenced by fertilization and crop response to …

Blackberry Virosome: A Micro And Macro Approach, Archana Khadgi

Graduate Theses and Dissertations

Viruses pose a major concern for blackberry production around the world with more than 40 species known to infect the crop. Virus complexes have been identified recently as the major cause of plant decline with blackberry yellow vein disease (BYVD) being the most important disease of the crop in the Southern United States. The objective of this research was to study the blackberry virosome in both the macro and micro scale. The large scale approach involves identification of the major viruses known to be associated with BYVD in the Southern United States as well as the identification of other viruses …

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …

Ecosystem Services Of Urban Trees And The Impacts Of Urbanization, Jorge E. Cantu

Theses and Dissertations - UTB/UTPA

The University of Texas- Pan American has conducted a complete survey of campus trees in partial fulfillment of the requirements for membership in the International Society of Arboriculture Designation of Tree Campus USA. This tree inventory was accomplished with the help of students whom were trained by faculty and foresters. Other than the completion of the tree inventory, this thesis had two main goals; 1) valuate the ecosystem services provided by campus trees 2) create a unique service learning project that other institutions can model. According to our calculations, the trees on campus have sequestered 568,652 kg of CO2, avoided …

Plant Species' Origin Predicts Dominance And Response To Nutrient Enrichment And Herbivores In Global Grasslands, Eric W. Seabloom, Elizabeth T. Borer, Yvonne M. Buckley, Elsa E. Cleland, Kendi F. Davies, Jennifer Firn, W. Stanley Harpole, Yann Hautier, Eric M. Lind, Andrew S. Macdougall, John L. Orrock, Suzanne M. Prober, Peter B. Adler, T. Michael Anderson, Jonathan D. Bakker, Lori A. Biederman, Dana M. Blumenthal, Cynthia S. Brown, Lars A. Brudvig, Marc Cadotte, Chengjin Chu, Kathryn L. Cottingham, Michael J. Crawley, Ellen I. Damschen, Carla M. Dantonio, Nicole M. Decrappeo, Guozhen Du, Philip A. Fay, Paul Frater, Daniel S. Gruner, Nicole Hagenah, Andy Hector, Helmut Hillebrand, Kirsten S. Hofmockel, Hope C. Humphries, Virginia L. Jin, Adam Kay, Kevin P. Kirkman, Julia A. Klein, Johannes M. H. Knops, Kimberly J. La Pierre, Laura Ladwig, John G. Lambrinos, Qi Li, Wei Li, Robin Marushia, Rebecca L. Mcculley, Brett A. Melbourne, Charles E. Mitchell, Joslin L. Moore, John Morgan, Brent Mortensen, Lydia R. O'Halloran, David A. Pyke, Anita C. Risch, Mahesh Sankaran, Martin Schuetz, Anna Simonsen, Melinda D. Smith, Carly J. Stevens, Lauren Sullivan, Elizabeth Wolkovich, Peter D. Wragg, Justin Wright, Louie Yang

Plant and Soil Sciences Faculty Publications

Exotic species dominate many communities; however the functional significance of species' biogeographic origin remains highly contentious. This debate is fuelled in part by the lack of globally replicated, systematic data assessing the relationship between species provenance, function and response to perturbations. We examined the abundance of native and exotic plant species at 64 grasslands in 13 countries, and at a subset of the sites we experimentally tested native and exotic species responses to two fundamental drivers of invasion, mineral nutrient supplies and vertebrate herbivory. Exotic species are six times more likely to dominate communities than native species. Furthermore, while experimental …

Soybean Seed Quality And Vigor: Influencing Factors, Measurement, And Pathogen Characterization, Kimberly Ann Cochran

Graduate Theses and Dissertations

High seed vigor (SV), or the ability of seed to germinate and grow in a stressful environment is important, as many field emergence conditions are stressful for germination. Soybean SV can be affected by cultivar, seed storage environment conditions, the growing environment during seed development, crop management practices such as foliar fungicide applications, pathogens, and other factors. To assess the effects of some of these factors on soybean, the objectives of this work were to determine 1. the effect of cultivar and foliar azoxystrobin application on soybean yield, germination, vigor, microflora, and their relationships under harvest delay conditions; 2. if …

Pythium: Characterization Of Resistance In Soybean And Population Diversity, Keiddy Esperanza Urrea Romero

Graduate Theses and Dissertations

Pythium spp. are an important group of pathogens causing stand losses in Arkansas soybean production. New inoculation methods and advances in molecular techniques allow a better understanding of cultivar resistance and responses of Pythium communities to cultural practices. The objectives of this research were to i) characterize the resistance of soybean to P. aphanidermatum with two phenotyping assays that evaluated the seed rot phase of the disease; and ii) understand the effect of long term crop rotation on species diversity and iii) to determine the effect of location, temperature and continuous soybean and soybean-rice rotation on Pythium spp. diversity in …

Investigations Of Ethylene And Ethylene Inhibition, Ball Lie, And Synthetic Turf Color, William Daniel Strunk

Graduate Theses and Dissertations

This dissertation is comprised of several studies over three unrelated areas of focus. Chapters two and three investigate the role of the hormone ethylene on growth of creeping bentgrass through (1) exposure of turf to exogenous ethylene and (2) testing an ethylene inhibiting compound for use to alleviate stress related injury. Previous research demonstrated a correlation of stress injury and ethylene and lessening of injury through ethylene inhibition, but little work has been conducted evaluating the direct effects of ethylene. These studies failed to confirm findings in previous research. Chapters four and five aim to illustrate the impact of turfgrass …

Long-Term Effects Of Alternative Residue Management Practices On Near-Surface Soil Properties And Soybean Production In A Wheat-Soybean, Double-Crop System In Eastern Arkansas, Christopher Ryan Norman

Graduate Theses and Dissertations

Adoption of management practices that maintain or increase soil organic matter (SOM), which contains 58% carbon (C) on average, may help to mitigate climate change by sequestering atmospheric C. Therefore, the main objective of this study was to determine the long-term trends in SOM, soil C and nitrogen (N), bulk density, various soil chemical properties (i.e., pH, electrical conductivity [EC], and Mehlich-3-extractable nutrients) in the top 10 cm, and soybean yield as affected by residue burning (burning and non-burning), tillage (conventional and no-tillage), irrigation (irrigated and non-irrigated), and N-fertilization/residue level (high and low) in a wheat (Triticum aestivum L.)-soybean [Glycine …

Temperature In The Cotton (Gossypium Hirsutum L.) Canopy And Effects On Cotton Leaf And Boll Growth, Maria Soledad Berlangieri Costa

Graduate Theses and Dissertations

Cotton development and yield are negatively influenced by extremes temperatures, mainly during reproductive stage. Ambient air temperature is used to evaluate temperature stress effect on yield under field conditions; however, there is evidence that actual temperatures in the canopy where bolls develop are different. In terms of cotton responses, there is limited research about the effects of elevated day and night temperatures upon boll carbohydrate content, size, and boll respiration. Field and growth chamber experiments were performed during 2014-2015 using the cotton (Gossypium hirsutum L.) cultivar DP0912B2RF. Field studies for determining temperature gradients through the canopy consisted of two planting …

Alliaria Petiolata (M.Bieb.) Cavara & Grande [Brassicaceae], An Invasive Herb In The Southern Ozark Plateaus: A Comparison Of Species Composition And Richness, Soil Properties, And Earthworm Composition And Biomass In Invaded Versus Non-Invaded Sites, Jennifer D. Ogle

Graduate Theses and Dissertations

Invasive species are widely recognized as organisms that severely alter ecosystem processes in the habitats to which they are introduced. Alliaria petiolata is one of the most important invasive plants in forests of the northern United States. This study examined the geographic distribution of the plant in the southern Ozarks, as well as the effect that it may be having on natural processes within forests of the region by comparing plant species richness, plant cover, and soil properties in invaded and non-invaded plots. It was found that A. petiolata is not significantly altering species richness, cover, or soil properties in …

Effect Of Nitrogen Fertilizer Rates And Two-Spotted Spider Mite Incidence On 'Strawberry Festival' Strawberry Cultivar Performance, Megan E. Mcgovern

Graduate Theses and Dissertations

The decline in strawberry (Fragaria x ananassa Duch.) production in Arkansas over the last 50 years has sparked an interest in the use of season extension techniques to improve yield and fruit quality. High tunnel strawberry production has the potential to become a profitable and sustainable production system; however, information on nutrient and pest management is limited.

The overall objective of this study was to determine the response of ‘St. Festival’ strawberry cultivar to different nitrogen fertilizer rates in order to develop a fertilization program for strawberries grown under high tunnel production systems. Experiments were conducted at the University of …

Physiological Responses Of Cotton Genotypes To Water-Deficit Stress During Reproductive Development, Cristiane Pilon

Graduate Theses and Dissertations

Drought stress is one of the major abiotic factors affecting crop growth and limiting production worldwide. Cotton genotypes vary in drought tolerance, and the effects of drought stress on the anatomy and physiology of cotton leaves and roots have been reported. However, information on physiological and metabolic processes of leaves and flowers of modern cotton cultivars under water-deficit stress during reproductive development is not well elucidated. It was hypothesized that water-deficit stress during squaring and flowering stages would impair stomatal conductance and photosynthetic efficiency of leaves, which consequently would result in osmotic adjustment through accumulation of compatible solutes, increased activity …

Functional Analysis Of The Mms19 Gene In Carica Papaya, Jorge A. Trujillo

Theses and Dissertations - UTB/UTPA

All organisms face DNA-damaging radiation in the form of UV light and must have mechanisms to repair or undo damage. Plants being stationary organisms must cope with damaging UV rays in a molecular fashion and so have developed many forms of resistance. MMS19 is implicated in DNA repair indirectly as a nuclear encoded gene that binds onto protein assembly complexes in the cytosol. A mutant within the SunUp variety of Carica papaya has a large deletion for MMS19 and presents a diminutive phenotype. Deficient DNA repair mechanisms will shunt cells out of the natural cell cycle and reduce cell proliferation …

Association Mapping For Elemental Concentration Resulting From Vegetative Stage Soil Waterlogging Stress In Wheat (Triticum Aestivum L.), Maria Nelly Arguello-Blanco

Graduate Theses and Dissertations

Soil waterlogging (WL) affects wheat production worldwide, including the southern of the U.S. Little is known about the genetics of tolerance mechanisms to WL, particularly of potentially toxic elements such as aluminum, iron and sodium. The objectives of this study were to use association mapping (AM) to identify SNP associated with the EC in the vegetative tissue of a set of 240 diverse soft winter wheat genotypes (AM panel) grown under field WL and to determine the impact of WL on wheat yield (GY) and yield components in a set 28 adapted cultivars (YT)

YT study showed reductions in GY …

A Microhabitat Assessment Of Five Species Of Invasive Plants In The Ozarks And Appalachians, Eric Hearth

Graduate Theses and Dissertations

Invasive species present a threat to native communities and their introduction and expansion can alter community structure and dynamics. Multiple approaches can be employed for invasive species management including prevention and detection. In this study, microhabitat assessments were conducted on colonies of five species of invasive plants, Alliaria petiolata (M. Bieb.) Cavara & Grande, Lespedeza cuneata (Dum. Cours.) G. Don, Lonicera japonica Thunb., Microstegium vimineum (Trin.) A. Camus, and Rosa multiflora Thunb. in the Ozark Plateau and Appalachians. Elevation, soil moisture, soil pH, light ratio, slope, aspect, distance to disturbance, as well as soil nutrient levels were recorded for each …