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The Effects Of Cold Treatment And Charcoal On The In Vitro Androgenesis Of Pepper (Capsicum Annuum L.), Dudu Özkum Çi̇ner, Ruki̇ye Tipirdamaz

Turkish Journal of Botany

The effects of cold-shock treatment of Capsicum annuum L. buds at 4°C for 48 or 96 h, and the addition of 0.25% activated charcoal on embryo formation were examined. Murashige and Skoog (MS) basal nutrient medium was supplemented with combinations of 4 mg l^{-1} naphthalene acetic acid (NAA) and 1 mg l^{-1} benzyladenine (BA) or 1 mg l^{-1} NAA and 4 mg l^{-1} BA, together with 0.8% (w/v) agar and 3% (w/v) sucrose. The highest number of embryos was obtained from the control anthers which were not subject to cold treatments and were cultured on MS medium containing 4 mg …