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Full-Text Articles in Life Sciences

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr Aug 1975

Pollution Of Surface Irrigation Waters By Plant Pathogenic Organisms, James R. Steadman, C. R. Maier, H. F. Schwartz, E. D. Kerr

Department of Plant Pathology: Faculty Publications

Systematic sampling of waterways and irrigation runoff from agricultural lands in the North Platte Project of Nebraska in July and August of 1972–1974 demonstrated that phytopathogenic organisms were disseminated. The organisms monitored included the bean common blight bacterium Xanthomonas phaseoli, the bean white mold fungus Whetzelinia sclerotiorum and various nematodes. Although many types of nematodes often were recovered from irrigation water, Heterodera sp. cysts which cause significant disease problems in the valley were found infrequently. Patterns of movement of the bacterial and fungal organisms were correlated with previous or current season infection of bean plants. The short-term survival of X. …


Intermediates In The Biosynthesis Of Double-Stranded Ribonucleic Acids Of Bacteriophage Φ6, D. L. Coplin, James L. Van Etten, James A. Koski, A. K. Vidaver Mar 1975

Intermediates In The Biosynthesis Of Double-Stranded Ribonucleic Acids Of Bacteriophage Φ6, D. L. Coplin, James L. Van Etten, James A. Koski, A. K. Vidaver

Department of Plant Pathology: Faculty Publications

Pseudomonas phaseolicola infected with bacteriophage φ6 synthesized all three viral double-stranded RNA segments, three single-stranded RNAs, and three replicative intermediate-like RNAs in the presence of rifampin. The single-stranded RNA intermediates sedimented and electrophoresed along with melted viral double-stranded RNA, annealed with melted viral double-stranded RNA, and were transient in nature. The relative amounts of the single-stranded RNA intermediates varied during the infection cycle and were altered in the presence of chloramphenicol. The replicative intermediate-like RNAs sedimented faster than double-stranded RNA, failed to enter 2.5% polyacrylamide gels, eluted with double-stranded RNA from a CF-11 cellulose column, were precipitated with single-stranded RNA …