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Enhancing Corn Yield In A Winter Cereal Rye Cover Crop System In Southwest Iowa, John Lundvall, Swetabh Patel, John Sawyer Mar 2017

Enhancing Corn Yield In A Winter Cereal Rye Cover Crop System In Southwest Iowa, John Lundvall, Swetabh Patel, John Sawyer

John E. Sawyer

Water quality impairment related to nitrogen (N) is a concern in Iowa, including meeting nitrate (NO3) drinking water standards and reducing the amount of N lost to the Gulf of Mexico. The Iowa Nutrient Reduction Strategy science assessment identified a rye cover crop as an important in-field management practice for reducing N and phosphorus (P) loss from fields (31% NO3-N and 29% P), and for reducing soil erosion. However, the science assessment identified a corn yield reduction of 6 percent when grown following a rye cover crop. Lower corn yield with use of a cover crop is unacceptable to farmers, …


Corn Nitrogen Fertilization Requirement And Corn–Soybean Productivity With A Rye Cover Crop, Jose L. Pantoja, Krishna P. Woli, John E. Sawyer, Daniel W. Barker Mar 2017

Corn Nitrogen Fertilization Requirement And Corn–Soybean Productivity With A Rye Cover Crop, Jose L. Pantoja, Krishna P. Woli, John E. Sawyer, Daniel W. Barker

John E. Sawyer

Winter rye (Secale cereale L.) cover crop (RCC) has potential to reduce NO3–N loss from corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] fields. However, RCC effects on annual crop productivity and corn optimal N fertilization requirement are unclear. The objectives were to evaluate corn and soybean yield response to RCC and corn optimal N rate. Treatments were no-RCC and RCC with six fertilizer N rates (0–225 kg N ha-1) applied to corn in a no-till corn–soybean (CS) rotation at four Iowa sites in 2009 through 2011. The RCC biomass and …


Standardized Research Protocols Enable Transdisciplinary Research Of Climate Variation Impacts In Corn Production Systems, E. J. Kladivko, M. J. Helmers, L. J. Abendroth, D. Herzmann, R. Lal, M. J. Castellano, D. S. Mueller, J. E. Sawyer, R. P. Anex, R. W. Arritt, B. Basso, J. V. Bonta, L. C. Bowling, R. M. Cruse, N. R. Fausey, J. R. Frankenberger, P. W. Gassman, A. J. Gassmann, C. L. Kling, A. Kravchenko, J. G. Lauer, F. E. Miguez, E. D. Nafziger, N. Nkongolo, M. O'Neal, L. B. Owens, P. R. Owens, P. Scharf, M. J. Shipitalo, J. S. Strock, M. B. Villamil Mar 2017

Standardized Research Protocols Enable Transdisciplinary Research Of Climate Variation Impacts In Corn Production Systems, E. J. Kladivko, M. J. Helmers, L. J. Abendroth, D. Herzmann, R. Lal, M. J. Castellano, D. S. Mueller, J. E. Sawyer, R. P. Anex, R. W. Arritt, B. Basso, J. V. Bonta, L. C. Bowling, R. M. Cruse, N. R. Fausey, J. R. Frankenberger, P. W. Gassman, A. J. Gassmann, C. L. Kling, A. Kravchenko, J. G. Lauer, F. E. Miguez, E. D. Nafziger, N. Nkongolo, M. O'Neal, L. B. Owens, P. R. Owens, P. Scharf, M. J. Shipitalo, J. S. Strock, M. B. Villamil

John E. Sawyer

The important questions about agriculture, climate, and sustainability have become increasingly complex and require a coordinated, multifaceted approach for developing new knowledge and understanding. A multistate, transdisciplinary project was begun in 2011 to study the potential for both mitigation and adaptation of corn-based cropping systems to climate variations. The team is measuring the baseline as well as change of the system's carbon (C), nitrogen (N), and water footprints, crop productivity, and pest pressure in response to existing and novel production practices. Nine states and 11 institutions are participating in the project, necessitating a well thought out approach to coordinating field …


Why Manage Phosphorus?, John E. Sawyer, Antonio P. Mallarino Mar 2017

Why Manage Phosphorus?, John E. Sawyer, Antonio P. Mallarino

John E. Sawyer

There are 17 nutrients that are essential for plant growth and production. An insufficient supply of one or more can have an adverse effect on plant growth, maturity, and yield. Phosphorus (P)--one of three key macronutrients (nitrogen and potassium are the other two)--is present in every living plant and animal cell, and is vital in plants for harvesting the sun's energy for growth and reproduction. This requirement is the same whether the plant is growing on soil or in water (such as algae); hence, the concern regarding P levels in surface water and the accelerated aquatic plant growth associated with …


Yellow Corn, Wet Soils, And Nitrogen Loss (Part 3), John E. Sawyer Mar 2017

Yellow Corn, Wet Soils, And Nitrogen Loss (Part 3), John E. Sawyer

John E. Sawyer

Earlier I provided observations in two articles (June 19 and June 26, 2008) on corn growth and response to nitrogen (N) applied in an anhydrous ammonia study being conducted at the ISU research farm between Ames and Boone. Following are some final observations (July 7, 2008).


Wait Until Soil Temps Drop Before Applying Anhydrous Ammonia, John E. Sawyer, Barbara Stewart, William Ehm Mar 2017

Wait Until Soil Temps Drop Before Applying Anhydrous Ammonia, John E. Sawyer, Barbara Stewart, William Ehm

John E. Sawyer

With the early harvest, Iowa’s conservation leaders are encouraging farmers to wait until soil temperatures lower before applying anhydrous ammonia (NH3) this fall. Anhydrous ammonia applied before daily soil temperatures remain below 50 degrees Fahrenheit and continue trending lower can result in the nitrogen loss that can impact crop development and have negative environmental impacts, such as enhanced leaching into groundwater and streams once converted to nitrate.


Wet Conditions And Change In Soil Profile Nitrate, John E. Sawyer Mar 2017

Wet Conditions And Change In Soil Profile Nitrate, John E. Sawyer

John E. Sawyer

I wrote an ICM News article February 21, 2013, that provided a summary of fall soil profile nitrate sampling results following the 2012 corn harvest. As I cautioned in that and other articles, the amount of nitrate-N that might remain for a 2013 corn crop depends on springtime rainfall. Unfortunately, much of Iowa has received considerable precipitation since soils thawed, especially the eastern two-thirds of Iowa. The two maps of the Midwest region show the total precipitation and deviation from normal since March 7, 2013. Tile lines are flowing again, and nitrate in the profile will move with percolating water. …


Sulfur Emerges As A Nutritional Issue In Iowa Alfalfa Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker Mar 2017

Sulfur Emerges As A Nutritional Issue In Iowa Alfalfa Production, John E. Sawyer, Brian J. Lang, Daniel W. Barker

John E. Sawyer

Sulfur is often classified as a “secondary” essential element, mainly due to a smaller plant requirement, but also because it is less frequently applied as a fertilizer compared to N, P, and K. This has certainly been the case in Iowa, where research had not documented S deficiency or fertilization need for optimal crop production. However, if deficient, S can have a dramatic effect on plant growth and crop productivity – more than the classification “secondary” would imply.


Uan And Preemergence Herbicide Applications On Emerged Corn, Michael D. Owen, John E. Sawyer, Robert G. Hartzler Mar 2017

Uan And Preemergence Herbicide Applications On Emerged Corn, Michael D. Owen, John E. Sawyer, Robert G. Hartzler

John E. Sawyer

Planting the Iowa 2007 corn crop is somewhat behind recent years, and in efforts to stay ahead of the game, many fields will be planted before being treated with nitrogen (N) and/or herbicides. Corn in some of these fields will emerge before applications of these materials are made, raising concerns over the safety of postemergence applications of urea-ammonium nitrate (UAN) solution, with or without herbicides.


Soybean Top Dieback Shows Up In Iowa Again, Xiao-Bing Yang, John E. Sawyer Mar 2017

Soybean Top Dieback Shows Up In Iowa Again, Xiao-Bing Yang, John E. Sawyer

John E. Sawyer

Extension field agronomists report the occurrence of soybean top dieback in northeastern Iowa soybean fields this week. Soybean top dieback symptoms have shown up occasionally since it was first reported by Jim Fawcett and Jerry Long in Cedar County a few years ago. Last year it was also found in eastern and central Iowa.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project is designed to study the N fertilization needs in continuous corn (C-C) and corn rotated with soybeans (C-S) as influenced by location and climate. Multiple rates of fertilizer N are spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation practice, differences that exist between the two rotations, and responses to applied N across different soils and climatic conditions. It will also allow for the evaluation of tools used to adjust …


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project was designed to study the N fertilization needs in continuous corn (CC) and corn rotated with soybean (SC) as influenced by location and climate. Multiple rates of fertilizer N were spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation practice, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project is designed to study the N fertilization needs in continuous corn (C-C) and corn rotated with soybean (C-S) as influenced by location and climate. Multiple rates of fertilizer N are spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will help determine N requirements for each rotation practice, differences that exist between the two rotations, responses to applied N across different soils and different climatic conditions. It will also allow for the evaluation of tools used to adjust N application.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker, John Lundvall Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker, John Lundvall

John E. Sawyer

This project is designed to study the N fertilization needs of continuous corn (C-C) and corn rotated with soybeans (C-S) as influenced by location and climate. Multiple rates of fertilizer N are spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will help determine N requirements for each rotational practice, differences that exist between the two rotations, and responses to applied N across different soils and different climatic conditions. It will also allow for the evaluation of tools used to adjust N …


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project was designed to study the N fertilization needs in continuous corn (CC) and corn rotated with soybean (SC) as influenced by location and climate. Multiple rates of fertilizer N were spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project was designed to study the N fertilization needs in continuous corn (CC) and corn rotated with soybean (SC) as influenced by location and climate. Multiple rates of fertilizer N were spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project was designed to study the N fertilization needs in continuous corn (CC) and corn rotated with soybean (SC) as influenced by location and climate. Multiple rates of fertilizer N are spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.


Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker Mar 2017

Seasonal And Rotational Influences On Corn Nitrogen Requirements, John E. Sawyer, Daniel W. Barker

John E. Sawyer

This project was designed to study the N fertilization needs in continuous corn (CC) and corn rotated with soybean (SC) as influenced by location and climate. Multiple rates of fertilizer N are spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow the determination of N requirements for each rotation practice, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.


Phosphorus And Potassium In Silage Harvest Of Drought-Damaged Corn, John E. Sawyer, Antonio P. Mallarino Mar 2017

Phosphorus And Potassium In Silage Harvest Of Drought-Damaged Corn, John E. Sawyer, Antonio P. Mallarino

John E. Sawyer

The dry conditions this summer are resulting in quite variable corn growth and production potential. Some livestock producers are beginning harvest of corn damaged by drought conditions for silage. Corn silage harvest results in more phosphorus (P) and potassium (K) removal than grain alone because almost the entire plant is harvested. The increased amount removed with silage differs for P and K because the relative amount of P and K is different in corn vegetative parts than in grain. For P there can be approximately four times greater amount of P per ton of dry matter in grain than vegetative …


Nitrogen Loss: How Does It Happen?, John E. Sawyer Mar 2017

Nitrogen Loss: How Does It Happen?, John E. Sawyer

John E. Sawyer

For the period April 1 through May 6, 2007, much of Iowa received more than 5 inches of rainfall (May 6, 2007 AWIS Inc. report). The Northwest, North-Central, and East-Central districts averaged slightly less than 5 inches, and the West-Central and Southwest districts more than 9 inches. With the large rainfall and flooding conditions, many producers are wondering about nitrogen (N) losses. While this period certainly has an influence on N in the soil, excessively wet conditions later in the spring are especially important for N losses. Early to mid-June will be a critical period to assess crop growth and …


Competition And Propagule Density Affect Sexual And Clonal Propagation Of A Weed, Daniel Z. Atwater, Wonjae Kim, Daniel R. Tekiela, Jacob N. Barney Feb 2017

Competition And Propagule Density Affect Sexual And Clonal Propagation Of A Weed, Daniel Z. Atwater, Wonjae Kim, Daniel R. Tekiela, Jacob N. Barney

Wonjae Kim

Many introduced species are capable of both sexual and vegetative reproduction. Our understanding of the ecology of such species depends on the trade-offs between vegetative and sexual reproduction and the ecological conditions that favor both modes of reproduction and how those factors influence the population ecology of introduced species. Here, we studied the efficacy of propagation via both seeds and rhizomes in Johnsongrass, a widespread invasive grass whose success is due to its prolific production of shattering seeds and rhizomes, the latter of which are readily dispersed by anthropogenic and natural processes. In a common garden in Virginia, we varied …


Assessing The Potential To Decrease The Gulf Of Mexico Hypoxic Zone With Midwest Us Perennial Cellulosic Feedstock Production, Andy Vanloocke, Tracy E. Twine, Christopher J. Kucharik, Carl J. Bernacchi Jan 2017

Assessing The Potential To Decrease The Gulf Of Mexico Hypoxic Zone With Midwest Us Perennial Cellulosic Feedstock Production, Andy Vanloocke, Tracy E. Twine, Christopher J. Kucharik, Carl J. Bernacchi

Andy VanLoocke

The goal of this research was to determine the changes in streamflow, dissolved inorganic nitrogen (DIN) leaching and export to the Gulf of Mexico associated with a range of large-scale dedicated perennial cellulosic bioenergy production scenarios within in the Mississippi–Atchafalaya River Basin (MARB). To achieve this goal, we used Agro-IBIS, a vegetation model capable of simulating the biogeochemistry of row crops, miscanthus and switchgrass, coupled with THMB, a hydrology model capable of simulating streamflow and DIN export. Simulations were conducted at varying fertilizer application rates (0–200 kg N ha -1) and fractional replacement (5–25%) of current row crops with miscanthus …


Expression Of Cyanobacterial Fbp/Sbpase In Soybean Prevents Yield Depression Under Future Climate Conditions, Iris H. Kohler, Ursula M. Ruiz-Vera, Andy Vanloocke, Michell L. Thomey, Tom Clemente, Stephen P. Long, Donald R. Ort, Carl J. Bernacchi Jan 2017

Expression Of Cyanobacterial Fbp/Sbpase In Soybean Prevents Yield Depression Under Future Climate Conditions, Iris H. Kohler, Ursula M. Ruiz-Vera, Andy Vanloocke, Michell L. Thomey, Tom Clemente, Stephen P. Long, Donald R. Ort, Carl J. Bernacchi

Andy VanLoocke

Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol−1) and elevated (600 μmol mol−1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 …


A Realistic Meteorological Assessment Of Perennial Biofuel Crop Deployment: A Southern Great Plains Perspective, Melissa Wagner, Meng Wang, Gonzalo Miguez-Macho, Jesse Miller, Andy Vanloocke, Justin E. Bagley, Carl J. Bernacchi, Matei Georgescu Jan 2017

A Realistic Meteorological Assessment Of Perennial Biofuel Crop Deployment: A Southern Great Plains Perspective, Melissa Wagner, Meng Wang, Gonzalo Miguez-Macho, Jesse Miller, Andy Vanloocke, Justin E. Bagley, Carl J. Bernacchi, Matei Georgescu

Andy VanLoocke

Utility of perennial bioenergy crops (e.g., switchgrass and miscanthus) offers unique opportunities to transition toward a more sustainable energy pathway due to their reduced carbon footprint, averted competition with food crops, and ability to grow on abandoned and degraded farmlands. Studies that have examined biogeophysical impacts of these crops noted a positive feedback between near-surface cooling and enhanced evapotranspiration (ET), but also potential unintended consequences of soil moisture and groundwater depletion. To better understand hydrometeorological effects of perennial bioenergy crop expansion, this study conducted high-resolution (2-km grid spacing) simulations with a state-of-the-art atmospheric model (Weather Research and Forecasting system) dynamically …


Impacts Of Second-Generation Biofuel Feedstock Production In The Central U.S. On The Hydrologic Cycle And Global Warming Mitigation Potential, K. J. Harding, T. E. Twine, Andy Vanloocke, J. E. Bagley, J. Hill Jan 2017

Impacts Of Second-Generation Biofuel Feedstock Production In The Central U.S. On The Hydrologic Cycle And Global Warming Mitigation Potential, K. J. Harding, T. E. Twine, Andy Vanloocke, J. E. Bagley, J. Hill

Andy VanLoocke

Biofuel feedstocks provide a renewable energy source that can reduce fossil fuel emissions; however, if produced on a large scale they can also impact local to regional water and carbon budgets. Simulation results for 2005–2014 from a regional weather model adapted to simulate the growth of two perennial grass biofuel feedstocks suggest that replacing at least half the current annual cropland with these grasses would increase water use efficiency and drive greater rainfall downwind of perturbed grid cells, but increased evapotranspiration (ET) might switch the Mississippi River basin from having a net warm-season surplus of water (precipitation minus ET) to …


Candidate Perennial Bioenergy Grasses Have A Higher Albedo Than Annual Row Crops, Jesse N. Miller, Andy Vanloocke, Nuria Gomez-Casanovas, Carl J. Bernacchi Jan 2017

Candidate Perennial Bioenergy Grasses Have A Higher Albedo Than Annual Row Crops, Jesse N. Miller, Andy Vanloocke, Nuria Gomez-Casanovas, Carl J. Bernacchi

Andy VanLoocke

The production of perennial cellulosic feedstocks for bioenergy presents the potential to diversify regional economies and the national energy supply, while also serving as climate ‘regulators’ due to a number of biogeochemical and biogeophysical differences relative to row crops. Numerous observational and model-based approaches have investigated biogeochemical trade-offs, such as increased carbon sequestration and increased water use, associated with growing cellulosic feedstocks. A less understood aspect is the biogeophysical changes associated with the difference in albedo (a), which could alter the local energy balance and cause local to regional cooling several times larger than that associated with offsetting carbon. Here, …


The Costs Of Photorespiration To Food Production Now And In The Future, Berkley J. Walker, Andy Vanloocke, Carl J. Bernacchi, Donald R. Ort Jan 2017

The Costs Of Photorespiration To Food Production Now And In The Future, Berkley J. Walker, Andy Vanloocke, Carl J. Bernacchi, Donald R. Ort

Andy VanLoocke

Photorespiration is essential for C3 plants but operates at the massive expense of fixed carbon dioxide and energy. Photorespiration is initiated when the initial enzyme of photosynthesis, ribulose-1,5-bisphosphate carboxylase/ oxygenase (Rubisco), reacts with oxygen instead of carbon dioxide and produces a toxic compound that is then recycled by photorespiration. Photorespiration can be modeled at the canopy and regional scales to determine its cost under current and future atmospheres. A regional-scale model reveals that photorespiration currently decreases US soybean and wheat yields by 36% and 20%, respectively, and a 5% decrease in the losses due to photorespiration would be worth approximately …


Evolution Of Floral Zygomorphy In Androecium And Corolla In Solanaceae, Jingbo Zhang, Peter F. Stevens, Wenheng Zhang Jan 2017

Evolution Of Floral Zygomorphy In Androecium And Corolla In Solanaceae, Jingbo Zhang, Peter F. Stevens, Wenheng Zhang

Peter Stevens

In Solanaceae, a group dominated by actinomorphic‐flowered species, floral zygomorphy is frequently observed among the early‐branching clades. Morphological studies indicated that a zygomorphic androecium is much more common than a zygomorphic corolla in the family. Ontogenic studies suggested the evolution of floral zygomorphy in these two whorls is independent. Here, we have examined the evolution of floral symmetry in the androecium and corolla in Solanaceae. The character states of floral symmetry were assembled for androecium and corolla separately, and ancestral state reconstructions were carried out at both the genus and species levels for Solanaceae and its outgroups. Correlation tests were …


Phospholipase Dζ Enhances Diacylglycerol Flux Into Triacylglycerol, Wenyu Yang, Geliang Wang, Jia Li, Philip David Bates, Xuemin Wang, Douglas K. Allen Jan 2017

Phospholipase Dζ Enhances Diacylglycerol Flux Into Triacylglycerol, Wenyu Yang, Geliang Wang, Jia Li, Philip David Bates, Xuemin Wang, Douglas K. Allen

Xuemin (Sam) Wang

Plant seeds are the primary source of triacylglycerols (TAG) for food, feed, fuel, and industrial applications. As TAG is produced from diacylglycerol (DAG), successful engineering strategies to enhance TAG levels have focused on the conversion of DAG to TAG. However, the production of TAG can be limited by flux through the enzymatic reactions that supply DAG. In this study, two Arabidopsis phospholipase Dζ genes (AtPLDζ1 and AtPLDζ2) were coexpressed in Camelina sativa to test whether the conversion of phosphatidylcholine to DAG impacts TAG levels in seeds. The resulting transgenic plants produced 2% to 3% more TAG as a component of …


Identification And Metabolite Profiling Of Chemical Activators Of Lipid Accumulation In Green Algae, Nishikant Wase, Boqiang Tu, Paul N. Black, Concetta C. Dirusso Dec 2016

Identification And Metabolite Profiling Of Chemical Activators Of Lipid Accumulation In Green Algae, Nishikant Wase, Boqiang Tu, Paul N. Black, Concetta C. Dirusso

Nishikant Wase

Microalgae are proposed as feedstock organisms useful for producing biofuels and coproducts. However, several limitations must be overcome before algae-based production is economically feasible. Among these is the ability to induce lipid accumulation and storage without affecting biomass yield. To overcome this barrier, a chemical genetics approach was employed in which 43,783 compounds were screened against Chlamydomonas reinhardtii, and 243 compounds were identified that increase triacylglyceride (TAG) accumulation without terminating growth. Identified compounds were classified by structural similarity, and 15 were selected for secondary analyses addressing impacts on growth fitness, photosynthetic pigments, and total cellular protein and starch concentrations. TAG …