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Chlorella Virus Atcv-1 Encodes A Functional Potassium Channel Of 82 Amino Acids, Sabrina Gazzarrini, Ming Kang, Alessandra Abenavoli, Giulia Romani, Claudio Olivari, Daniele Gaslini, Giuseppina Ferrara, James L. Van Etten, Michael Kreim, Stefan M. Kast, Gerhard Thiel, Anna Moroni
Chlorella Virus Atcv-1 Encodes A Functional Potassium Channel Of 82 Amino Acids, Sabrina Gazzarrini, Ming Kang, Alessandra Abenavoli, Giulia Romani, Claudio Olivari, Daniele Gaslini, Giuseppina Ferrara, James L. Van Etten, Michael Kreim, Stefan M. Kast, Gerhard Thiel, Anna Moroni
Department of Plant Pathology: Faculty Publications
Chlorella virus PBCV-1 (Paramecium bursaria chlorella virus-1) encodes the smallest protein (94 amino acids, named Kcv) previously known to form a functional K+ channel in heterologous systems. In this paper, we characterize another chlorella virus encoded K+ channel protein (82 amino acids, named ATCV-1 Kcv) that forms a functional channel in Xenopus oocytes and rescues Saccharomyces cerevisiae mutants that lack endogenous K+ uptake systems. Compared with the larger PBCV-1 Kcv, ATCV-1 Kcv lacks a cytoplasmic N-terminus and has a reduced number of charged amino acids in its turret domain. Despite these deficiencies, ATCV-1 Kcv accomplishes all the major features of …