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Articles 31 - 58 of 58
Full-Text Articles in Life Sciences
Induced Plant Mutation And Molecular Analysis As A Route To Genetic Improvement Of Specific Grain Quality Traits, L Slade Lee, Liz Izquierdo, Gary A. Ablett, Nicole F. Rice, Robert J. Henry
Induced Plant Mutation And Molecular Analysis As A Route To Genetic Improvement Of Specific Grain Quality Traits, L Slade Lee, Liz Izquierdo, Gary A. Ablett, Nicole F. Rice, Robert J. Henry
Dr Nicole F Rice
No abstract provided.
Genetic Diversity And Reproductive Biology In Warea Carteri (Brassicaceae), A Narrowly Endemic Florida Scrub Annual, Margaret E.K. Evans, Rebecca W. Dolan, Eric S. Menges, Doria R. Gordon
Genetic Diversity And Reproductive Biology In Warea Carteri (Brassicaceae), A Narrowly Endemic Florida Scrub Annual, Margaret E.K. Evans, Rebecca W. Dolan, Eric S. Menges, Doria R. Gordon
Rebecca W. Dolan
Carter's mustard (Warea carteri) is an endangered, fire-stimulated annual endemic of the Lake Wales Ridge, Florida, USA. This species is characterized by seed banks and large fluctuations in plant numbers, with increases occurring in postdisturbance habitat. We investigated the mating system, patterns of isozyme variation, and effective population sizes of W. carteri to better understand its population biology and to comment on reserve designs and management proposals relevant to this species. Warea carteri is self-compatible and autogamous, and probably largely selfing. Measures of genetic variation in W. carteri were lower than values reported for species with similar ecological and life …
Nomenclatural Changes For Some Grasses In California And The Muhlenbergia Clade (Poaceae), James Payne Smith Jr.
Nomenclatural Changes For Some Grasses In California And The Muhlenbergia Clade (Poaceae), James Payne Smith Jr.
James Payne Smith
No abstract provided.
Population Panmixia In A Spotted Gum Species Complex (Mytaceae): Implications For Association Mapping, Joel W. Ochieng, Peter R. Baverstock, Robert J. Henry, Mervyn Shepherd
Population Panmixia In A Spotted Gum Species Complex (Mytaceae): Implications For Association Mapping, Joel W. Ochieng, Peter R. Baverstock, Robert J. Henry, Mervyn Shepherd
Dr Mervyn Shepherd
No abstract provided.
The Australian Plant Dna Bank, Nicole F. Rice, Maurizio Rossetto, Giovanni M. Cordeiro, L Slade Lee, Effie M. Ablett, Robert J. Henry
The Australian Plant Dna Bank, Nicole F. Rice, Maurizio Rossetto, Giovanni M. Cordeiro, L Slade Lee, Effie M. Ablett, Robert J. Henry
Dr Nicole F Rice
The Australian Plant DNA Bank (dnabank.com.au) is an innovative approach to ex-situ preservation of genetic material from plants. The bank is a unique facility, which will extract and store plant DNA. The genetic material from plants provides endless information about each species and this is largely unexplored. Genomic DNA will be available to groups, on request, for research into gene discovery and function; marker development and detailed genotypic characterisation of plant varieties. For economically important crop species (eg. agricultural crops and forestry) the strategy is to include representatives from each species and/or variety. For other species, eg. native flora, it …
Genotyping Of The Fragrance Allele In Rice, Louis Mt Bradbury, Robert J. Henry, Qingsheng Jin, Russell F. Reinke, Daniel Le Waters
Genotyping Of The Fragrance Allele In Rice, Louis Mt Bradbury, Robert J. Henry, Qingsheng Jin, Russell F. Reinke, Daniel Le Waters
Adjunct Associate Professor Daniel LE Waters
We have previously determined that fragrance in rice, a recessive trait, is due to a large deletion (8bp) and 3 SNP’s in a gene on chromosome 8 which encodes a putative betaine aldehyde dehydrogenase 2 (BAD2). This mutation results in the formation of a truncated BAD2 enzyme because of the creation of an in-frame termination signal 800bp before that of the wild type. Because this truncated BAD2 is missing key binding domains, it is unlikely that it is capable of acting upon the target substrate and this leads to an accumulation of the principal fragrant molecule, 2-acetyl-1-pyrroline. Here we utilise …
Rice Genome Sequence Accelerates The Discovery Of Commercially Important Genes And Polymorphisms, Daniel Le Waters, Louis Mt Bradbury, Russell F. Reinke, Melissa A. Fitzgerald, Qingsheng Jin, Robert J. Henry
Rice Genome Sequence Accelerates The Discovery Of Commercially Important Genes And Polymorphisms, Daniel Le Waters, Louis Mt Bradbury, Russell F. Reinke, Melissa A. Fitzgerald, Qingsheng Jin, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Traditionally, identification of genes which control important traits has been labour intensive and time consuming. We have demonstrated the annotated rice genome sequence used in combination with re-sequencing by PCR greatly facilitates the discovery of both genes and polymorphisms within genes, which control commercially important traits. Identification of the gene which controls fragrance was achieved using a relatively small mapping population of 168 F2 individuals. Analysis of the recombination data and the relatively large tract (385 kbp) of annotated genome sequence between the flanking markers revealed a candidate gene in this region which plausibly explained the known biochemistry of fragrance. …
Scaming: An Efficient High Throughput Approach To Discovery And Analysis Of Snps For Genotyping Of Tropical Crop Species, Frances G. Eliott, Giovanni M. Cordeiro, Nicole F. Rice, James K. Cronin, Daniel Le Waters, Peter C. Bundock, Stirling Bowen, Robert J. Henry
Scaming: An Efficient High Throughput Approach To Discovery And Analysis Of Snps For Genotyping Of Tropical Crop Species, Frances G. Eliott, Giovanni M. Cordeiro, Nicole F. Rice, James K. Cronin, Daniel Le Waters, Peter C. Bundock, Stirling Bowen, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Model organisms have well established genetic marker tools. However, genotyping of the wide range of agricultural and food species requires the discovery of genetic variations in each species that can be used to distinguish genotypes. Single Nucleotide Polymorphisms (SNPs) are the basis of most high throughput genetic analysis technologies. We have developed a generic approach to the discovery and analysis of SNPs in non-model organisms. SNP Characterisation and Mapping in Genomes (SCAMinG) has been developed from mutation detection based on enzymatic cleavage of DNA heteroduplexes as applied to naturally occurring genetic variation (ecoTILLinG). Our protocol involves application of high throughput …
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Rice grains exhibit unique starch properties and these properties differ among varieties. Starch granule morphology influences the physicochemical characteristics of starch and granule shape/size are a contributing factor in starch functional properties. Wild rice relatives represent a diverse range of material that may be useful for the identification of novel end-products or identify new germplasm for breeding programs. Comparative scanning electron microscopy studies can reveal differences in starch granule morphology between species. This study presents a qualitative evaluation of starch granule morphology of 19 spp. from four different genera (Oryza, Potamophila, Microlaena and Zizania) belonging to two different tribes (Oryzeae, …
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Dr Nicole F Rice
Rice grains exhibit unique starch properties and these properties differ among varieties. Starch granule morphology influences the physicochemical characteristics of starch and granule shape/size are a contributing factor in starch functional properties. Wild rice relatives represent a diverse range of material that may be useful for the identification of novel end-products or identify new germplasm for breeding programs. Comparative scanning electron microscopy studies can reveal differences in starch granule morphology between species. This study presents a qualitative evaluation of starch granule morphology of 19 spp. from four different genera (Oryza, Potamophila, Microlaena and Zizania) belonging to two different tribes (Oryzeae, …
Scaming: An Efficient High Throughput Approach To Discovery And Analysis Of Snps For Genotyping Of Tropical Crop Species, Frances G. Eliott, Giovanni M. Cordeiro, Nicole F. Rice, James K. Cronin, Daniel Le Waters, Peter C. Bundock, Stirling Bowen, Robert J. Henry
Scaming: An Efficient High Throughput Approach To Discovery And Analysis Of Snps For Genotyping Of Tropical Crop Species, Frances G. Eliott, Giovanni M. Cordeiro, Nicole F. Rice, James K. Cronin, Daniel Le Waters, Peter C. Bundock, Stirling Bowen, Robert J. Henry
Dr Nicole F Rice
Model organisms have well established genetic marker tools. However, genotyping of the wide range of agricultural and food species requires the discovery of genetic variations in each species that can be used to distinguish genotypes. Single Nucleotide Polymorphisms (SNPs) are the basis of most high throughput genetic analysis technologies. We have developed a generic approach to the discovery and analysis of SNPs in non-model organisms. SNP Characterisation and Mapping in Genomes (SCAMinG) has been developed from mutation detection based on enzymatic cleavage of DNA heteroduplexes as applied to naturally occurring genetic variation (ecoTILLinG). Our protocol involves application of high throughput …
Plant Dna Banks And The Role In Plant Genomics, Nicole F. Rice, Loraine Watson, Robert J. Henry
Plant Dna Banks And The Role In Plant Genomics, Nicole F. Rice, Loraine Watson, Robert J. Henry
Dr Nicole F Rice
No abstract provided.
Targeted Mutagenesis In Sorghum Using An Improved High-Throughput Screening Platform – A Reverse Genetics Strategy To Complement The Sorghum Genomics Effort, Michael J. Cross, L Slade Lee, Nicole F. Rice, Robert J. Henry
Targeted Mutagenesis In Sorghum Using An Improved High-Throughput Screening Platform – A Reverse Genetics Strategy To Complement The Sorghum Genomics Effort, Michael J. Cross, L Slade Lee, Nicole F. Rice, Robert J. Henry
Dr Nicole F Rice
As a natural complement to the Sorghum Genomics effort, our group has undertaken a reverse genetics strategy involving point-mutagenesis, followed by CEL I mismatch screening. EMS-mutated populations have been generated in four white Sorghum lines. Our research has further developed the mutation screening technique of ‘CEL I mismatch detection’. Our modified technique is a significant improvement on the standard protocols (originally optimized for slab gel systems). Our protocol benefits from the increased sensitivity offered by a capillary electrophoresis platform, enabling a larger degree of sample pooling. Throughput has also been improved via a significant reduction in the amount of background …
Understanding Gene Expression In The Developing Grain, Toni Pacey-Miller, Loraine Watson, Jessica F. White, Shane R. Mcintosh, Allison C. Crawford, Peter C. Bundock, Daniel Barbary, Giovanni M. Cordeiro, Robert J. Henry
Understanding Gene Expression In The Developing Grain, Toni Pacey-Miller, Loraine Watson, Jessica F. White, Shane R. Mcintosh, Allison C. Crawford, Peter C. Bundock, Daniel Barbary, Giovanni M. Cordeiro, Robert J. Henry
Professor Robert J Henry
Serial Analysis of Gene Expression (SAGE) has been used to understand more comprehensively the gene expression involved in the growth of a developing wheat grain. SAGE is a technique that allows rapid, detailed analysis of thousands of transcripts in a cell. The abundance of a particular tag relates directly to the expression level of the gene from which it is derived. The analysis of gene expression of different physiological states provides both qualitative and quantitative information. Seed development is a complex process involving both the up and down regulation of many genes. Samples of the developing grain were taken at …
Competitive Allele Specific Pcr Molecular Markers For Rice [Oryza Sativa] Starch Gelatinisation Temperature, Daniel Le Waters, Robert J. Henry, Russell F. Reinke
Competitive Allele Specific Pcr Molecular Markers For Rice [Oryza Sativa] Starch Gelatinisation Temperature, Daniel Le Waters, Robert J. Henry, Russell F. Reinke
Professor Robert J Henry
No abstract provided.
Serial Analysis Of Gene Expression Of Malting Barley, Jessica F. White, Toni Pacey-Miller, Allison C. Crawford, Giovanni M. Cordeiro, Daniel Barbary, Peter C. Bundock, Robert J. Henry
Serial Analysis Of Gene Expression Of Malting Barley, Jessica F. White, Toni Pacey-Miller, Allison C. Crawford, Giovanni M. Cordeiro, Daniel Barbary, Peter C. Bundock, Robert J. Henry
Professor Robert J Henry
No abstract provided.
Malting Quality Improvement Using Sage, Toni Pacey-Miller, Loraine Watson, Jessica F. White, Allison C. Crawford, Peter C. Bundock, Giovanni M. Cordeiro, Daniel Barbary, Shane R. Mcintosh, Robert J. Henry
Malting Quality Improvement Using Sage, Toni Pacey-Miller, Loraine Watson, Jessica F. White, Allison C. Crawford, Peter C. Bundock, Giovanni M. Cordeiro, Daniel Barbary, Shane R. Mcintosh, Robert J. Henry
Dr Peter Bundock
No abstract provided.
Improving Cereals For Human Health, Shane R. Mcintosh, Daniel Le Waters, Louis Mt Bradbury, Timothy L. Fitzgerald, Robert J. Henry
Improving Cereals For Human Health, Shane R. Mcintosh, Daniel Le Waters, Louis Mt Bradbury, Timothy L. Fitzgerald, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
No abstract provided.
Competitive Allele Specific Pcr Molecular Markers For Rice [Oryza Sativa] Starch Gelatinisation Temperature, Daniel Le Waters, Robert J. Henry, Russell F. Reinke
Competitive Allele Specific Pcr Molecular Markers For Rice [Oryza Sativa] Starch Gelatinisation Temperature, Daniel Le Waters, Robert J. Henry, Russell F. Reinke
Adjunct Associate Professor Daniel LE Waters
No abstract provided.
Improving Rice For Human Health, Daniel Le Waters, Shane R. Mcintosh, Louis Mt Bradbury, Timothy L. Fitzgerald, Robert J. Henry
Improving Rice For Human Health, Daniel Le Waters, Shane R. Mcintosh, Louis Mt Bradbury, Timothy L. Fitzgerald, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
No abstract provided.
Cel I And The Email Mutation Scanning Assay, Michael J. Cross, Daniel Le Waters, L Slade Lee, Robert J. Henry
Cel I And The Email Mutation Scanning Assay, Michael J. Cross, Daniel Le Waters, L Slade Lee, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Scanning for unknown mutations has been advanced by mismatch cleavage techniques. Pioneering methods include the use of ribonucleases, chemical cleavage of mismatch (CCM) and T4 endonuclease VII. Recently discovered enzymes, such as Endonuclease V from Thermotoga maritima and CEL I, have further advanced mismatch detection, due to a combination of useful properties. These include high specificity for a wide range of substrates, improved sensitivity for detecting a low level of mutants, and simple means of application. Standard approaches involve the PCR amplification of sample alleles using 5’-labeled primers and the size fractionation of digestion products by LIF electrophoresis. Such protocols, …
Analysis Of Bad Gene Expression In Oryza Sativa Using Qrtpcr, Timothy L. Fitzgerald, Daniel Le Waters, Robert J. Henry
Analysis Of Bad Gene Expression In Oryza Sativa Using Qrtpcr, Timothy L. Fitzgerald, Daniel Le Waters, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Fragrance in rice (Oryza sativa), known to be primarily associated with grain 2-Acetyl-1-Pyrroline (2AP) concentration, is a highly valued trait. It has previously been determined that a non-functional betaine aldehyde dehydrogenase (BAD2) encoding gene homologue is responsible for fragrance in rice. It is hypothesized that in non-fragrant rice BAD2 catalyses the breakdown of a precursor to 2AP while the presence of a non-functional BAD2 encoding gene in fragrant varieties leads to an increase in the concentration of this precursor and a consequent increase in the concentration of 2AP. Functional BAD1 encoding genes have been identified in both fragrant and non-fragrant …
Generation Of Simple, Perfect Markers For Cereal Quality Traits, Louis Mt Bradbury, Daniel Le Waters, Robert J. Henry
Generation Of Simple, Perfect Markers For Cereal Quality Traits, Louis Mt Bradbury, Daniel Le Waters, Robert J. Henry
Adjunct Associate Professor Daniel LE Waters
Commercially important traits including fragrance and starch gelatinisation temperature (GT) are difficult phenotypes for breeders to asses. We recently identified polymorphisms that control these key rice quality traits. The annotated rice genome sequence used in combination with re-sequencing by PCR facilitated the discovery of these polymorphisms and the design of perfect molecular markers for each of these traits. In the absence of a genome sequence, larger mapping populations, a genome library and more sequencing would have been necessary. Although these traits result from different classes of polymorphism, fragrance is due to an eight base pair deletion while gelatinisation temperature variation …
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Evaluation Of Starch Granule Morphology In Wild Relatives Of Rice Using Scanning Electron Microscopy, Shabana Kasem, Daniel Le Waters, Nicole F. Rice, Robert J. Henry
Dr Nicole F Rice
Rice grains exhibit unique starch properties and these properties differ among varieties. Starch granule morphology influences the physicochemical characteristics of starch and granule shape/size are a contributing factor in starch functional properties. Wild rice relatives represent a diverse range of material that may be useful for the identification of novel end-products or identify new germplasm for breeding programs. Comparative scanning electron microscopy studies can reveal differences in starch granule morphology between species. This study presents a qualitative evaluation of starch granule morphology of 19 spp. from four different genera (Oryza, Potamophila, Microlaena and Zizania) belonging to two different tribes (Oryzeae, …
Genetic Change Following Fire In Populations Of A Seed-Banking Perennial Plant, Rebecca W. Dolan, Pedro F. Quintana-Ascencio, Eric S. Menges
Genetic Change Following Fire In Populations Of A Seed-Banking Perennial Plant, Rebecca W. Dolan, Pedro F. Quintana-Ascencio, Eric S. Menges
Rebecca W. Dolan
Disturbances such as fire have the potential to remove genetic variation, but seed banks may counter this loss by restoring alleles through a reservoir effect. We used allozyme analysis to characterize genetic change in two populations of the perennial Hypericum cumulicola, an endemic of the fire-prone Florida scrub. We assessed genetic variation before and 1, 2, and 3 years after fire that killed nearly all aboveground plants. Populations increased in size following fire, with most seedlings likely recruited from a persistent seed bank. Four of five loci were variable. Most alleles were present in low frequencies, but our large sample …
Induced Plant Mutation And Molecular Analysis As A Route To Genetic Improvement Of Specific Grain Quality Traits, L Slade Lee, Liz Izquierdo, Nicole F. Rice, Robert J. Henry, Gary A. Ablett
Induced Plant Mutation And Molecular Analysis As A Route To Genetic Improvement Of Specific Grain Quality Traits, L Slade Lee, Liz Izquierdo, Nicole F. Rice, Robert J. Henry, Gary A. Ablett
Dr Nicole F Rice
No abstract provided.
The Effect Of Water And Nitrogen On The Response Of Sorghum Cultivars With Contrasting Nitrogen Use Efficiency, Teshome Regassa
The Effect Of Water And Nitrogen On The Response Of Sorghum Cultivars With Contrasting Nitrogen Use Efficiency, Teshome Regassa
Teshome H. Regassa
Water and nitrogen (N) are important environmental factors limiting sorghum production. Information is lacking on the combined effects of water and N for cultivars with different degrees of water and/or N stress tolerance. Sorghum cultivars known for N and water stress tolerance difference were evaluated to identify plant characteristics contributing to N use efficiency (NUE). Cultivars San Chi San and CK-60 were evaluated at Mead, NE on a Sharpsburg silty clay loam soil under water (stress and non-stress), and N (0, 40, 80, and 120 kg ha−1) rates. Experimental units were arranged in split-split plot and treatments were …
Attendance Control Techniques: Union Vs. Non-Union Differences In The Southeast United States, S E. Markham, Dow Scott
Attendance Control Techniques: Union Vs. Non-Union Differences In The Southeast United States, S E. Markham, Dow Scott
Dow Scott
This research examines both the rate of absenteeism and the attendance control methods found in a sample of 423 union and non-union organizations located in the Southeast United States. These data indicate that absenteeism rates for union and non-union organizations are not significantly different. Methods of controlling absenteeism are reported for both union and non-union facilities. Implications for the control of absenteeism are discussed.