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Full-Text Articles in Life Sciences

Effects Of Chemical Variation On Competition And Insect Communities Across Solidago Altissima Genotypes, Bryan Scott Foster Ii Jan 2020

Effects Of Chemical Variation On Competition And Insect Communities Across Solidago Altissima Genotypes, Bryan Scott Foster Ii

Masters Theses

Individuals within a plant species can differ greatly from one another, especially regarding the range of chemical compounds produced. However, the functions of many of these chemicals are unknown, but likely include defenses against herbivores, attractants for pollinators and seed dispersers, as well as mechanisms for resource competition. To assess the effects of foliar chemical composition on interspecific plant competition and insect communities, I conducted a common garden and greenhouse experiment using 24 genotypes of the allelopathic species Solidago altissima for which the foliar chemistry had been characterized. Using these data and chemical profiles of S. altissima, I linked foliar ...


Nutrient Conservation Trait Responses Of Low Resource Adapted Chaparral Shrubs To Increased Resource Availability, Reina Lee Nielsen Jan 2018

Nutrient Conservation Trait Responses Of Low Resource Adapted Chaparral Shrubs To Increased Resource Availability, Reina Lee Nielsen

Masters Theses

Traits that increase mean nutrient retention times are essential to plant performance in low resource environments, where multiple stressors (low water and nutrients) are present. Although physiological responses to either water or nutrient stress are well understood, fewer studies have investigated the interaction of these stressors from a phylogenetically-controlled, whole-plant perspective. This research focused on three, phylogenetically-controlled pairs of shrubby evergreen species from the California chaparral that either grow on or off serpentine soils, which differ in nutrient availability. Using greenhouse and field studies, the responses of these plants to altered water and nutrient availability were evaluated. The greenhouse study ...


Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang Oct 2017

Uncovering Tasselsheath3. A Genomic And Phenotypic Analysis Of A Maize Floral Mutant., Thompson Zhang

Masters Theses

In the modern era, maize has become the most successful crop grown in the United States. According to the USDA over 90 million acres of land are planted to corn and 96.2% of the U.S feed grain production is made up of the cereal. Part of the success of maize is due to its floral architecture, and its pollination technique in which the flower opens, exposing stamens containing pollen into the air. A unique organ called the lodicule functions as a release mechanism, forcing the flower to open. Lodicules from grasses and eudicot petals are homologous, yet there ...


Protein, Methionine, And Cysteine Upregulation In Phaseolus Vulgaris ‘Black Turtle Bean’ Seeds Through Sulfur Fertilization At V2 And R2 Stages Of Growth, Hannah Paige Barry May 2017

Protein, Methionine, And Cysteine Upregulation In Phaseolus Vulgaris ‘Black Turtle Bean’ Seeds Through Sulfur Fertilization At V2 And R2 Stages Of Growth, Hannah Paige Barry

Masters Theses

The purpose of this research was to increase protein, methionine, and cysteine content in Phaseolus vulgaris L., common bean in relation to the inhibitory compound tannin. Previous research has shown that sulfur fertilization increases total protein, methionine and cysteine content in various crops, but always in tandem with inhibitory compound increases. If successful, the resulting bean seed will have a better nutrient profile for malnourished populations around the world.

Granular gypsum was applied at 0 kg S ha-1 [kilograms of sulfur per hectare], 10 kg S ha-1, 20 kg S ha-1, 40 kg S ha-1, 60 ...


Evaluation Of Hall’S Panicgrass (Panicum Hallii Vasey) As A Model System For Genetic Modification Of Recalcitrance In Switchgrass (Panicum Virgatum (L.)), Joshua Nathaniel Grant May 2017

Evaluation Of Hall’S Panicgrass (Panicum Hallii Vasey) As A Model System For Genetic Modification Of Recalcitrance In Switchgrass (Panicum Virgatum (L.)), Joshua Nathaniel Grant

Masters Theses

While switchgrass (Panicum virgatum (L.)) has long been recognized as a viable bioenergy feedstock, it and other plants have cell walls with recalcitrance to processing. Recalcitrance is recognized as a major barrier to broad adoption of switchgrass and other feedstocks for cellulosic bioenergy. In an effort to reduce recalcitrance, transgenic plants have been generated with altered cell wall phenotypes such as reduced lignin. Unfortunately, stable transformation of switchgrass and other C4 grasses is time intensive, costly, and genetic analysis is further complicated by polyploid genomic structures. Unlike switchgrass, which can be tetraploid to octoploid, a closely related species, Hall’s ...


Comparative Leaf Anatomy Of C3 And Cam Species In Oncidiinae (Maxillarieae, Orchidaceae), Samuel A. Eident Jan 2017

Comparative Leaf Anatomy Of C3 And Cam Species In Oncidiinae (Maxillarieae, Orchidaceae), Samuel A. Eident

Masters Theses

The subtribe Oncidiinae is a diverse group of mostly epiphytic orchids within the tribe Maxillarieae, consisting of 55 genera and 1700 species. In this group, there have been many studies examining morphological and anatomical variation as well as metabolic pathways of carbon fixation, but most have not integrated morphological and anatomical variation with a physiological aspect of inquiry. The objective of my research was to establish a suite of anatomical characteristics that can be used to distinguish between C3 and CAM species. Secondarily, I hoped to use that suite of characteristics in determining whether intermediate CAM species (those that ...


Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins Dec 2016

Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins

Masters Theses

Protoplasts are appropriate targets for genome editing, DNA functional screens and transgenesis. This project focuses on the comparison of inexpensive mesophyll protoplast isolation via the use of food-grade enzymes and transformation between diploid Panicum hallii Vasey (PAH) and polyploid cellulosic feedstock Panicum virgatum L (switchgrass), a relative of PAH. PAH has great potential as a C4 model species for crop and bioenergy research. Here an inexpensive switchgrass and PAH mesophyll protoplast isolation and transformation system was developed; the first protoplast system for PAH. Using low-cost commercial food-grade enzymes, a cost reduction of ~1000-fold was achieved compared to traditional protoplast isolating ...


Iron Signaling In Arabidopsis Thaliana, Celina Abundis Nov 2016

Iron Signaling In Arabidopsis Thaliana, Celina Abundis

Masters Theses

Iron is among the essential micronutrients for all living organisms and is a cofactor for many cellular redox reactions. Although iron is a highly abundant metal element found in the earth’s crust, it is also a limiting factor in plant development when it is present as insoluble ferric oxides. Plants have evolved two strategies to acquire soluble iron referred to as Strategy I and Strategy II. Our lab has focused on the Arabidopsis thaliana double mutant ysl1ysl3. The mutants display a chlorotic phenotype and are unable to correctly respond to iron deficiency.

Grafting is a common method for joining ...


Testing The Temporal Stability Of The Climate Response Of Tree Species At Norris Dam State Park, Tennessee, U.S.A., Allison Elizabeth Ingram Aug 2016

Testing The Temporal Stability Of The Climate Response Of Tree Species At Norris Dam State Park, Tennessee, U.S.A., Allison Elizabeth Ingram

Masters Theses

Temporal stability of the climate-tree growth relationship means that over time, tree species were responding to a specific climate variable and continue to respond to that variable into the present. The stability of this response is important to test prior to attempting to reconstruct past climate. In this study, I sampled oaks (white oak = Quercus alba L. and chestnut oak = Quercus montana Willd.) and pines (Virginia pine = Pinus virginiana Mill. and shortleaf pine = Pinus echinata Mill.) growing in Norris Dam State Park in eastern Tennessee and tested the temporal stability of these species and their potential for reconstructing past climate ...


Rooting Characteristics And Antioxidant Pigment Responses Of Three Hybrid Bermudagrass [Cynodon Dactylon (L.) Pers. × Cynodon Transvaalensis Burtt- Davy] Cultivars Exposed To Drought, Cory Alan Yurisic May 2016

Rooting Characteristics And Antioxidant Pigment Responses Of Three Hybrid Bermudagrass [Cynodon Dactylon (L.) Pers. × Cynodon Transvaalensis Burtt- Davy] Cultivars Exposed To Drought, Cory Alan Yurisic

Masters Theses

With recent changes in global climate, water use and its conservation have become important topics of discussion. Turfgrasses for lawns, parks, and other recreational areas are often perceived negatively due to the need for irrigation. The identification of turfgrasses possessing an improved ability to withstand water deficit is an important goal in science to curb irrigation requirements in the landscape. Newly released cultivars of hybrid bermudagrass (C. dactylon L. x. C. transvaalensis Burtt-Davy) have demonstrated improved tolerance to stresses such as cold, traffic, and water deficit; however, drought resistance mechanisms of these new cultivars have been largely uninvestigated. Drought resistant ...


Variations In The Invertebrate Communities Of Wild Cape Cod Cranberry Bogs, Barbara Wagner Mar 2016

Variations In The Invertebrate Communities Of Wild Cape Cod Cranberry Bogs, Barbara Wagner

Masters Theses

As a species domesticated only in the last century, agricultural cranberry plants (Vaccinium macrocarpon) remain little removed from their wild relatives. Thus, it is a potential model species for studies of the earliest stages of domestication; however, there is little available quantitative information on its wild population biology and ecology. As such information is vital to studies of the ecological changes occurring during domestication, the purpose of this study was to consolidate the relevant knowledge available and conduct a preliminary search for patterns in the invertebrate communities of wild bogs. The alpha diversity was found to be greater than the ...


Successional Changes In Leaf Nutrient Concentrations Of Woody And Herbaceous Species, Daniel Walter Kuchta Jan 2016

Successional Changes In Leaf Nutrient Concentrations Of Woody And Herbaceous Species, Daniel Walter Kuchta

Masters Theses

Leaf nutrient concentrations are an example of a functional trait, a trait that can be used to understand community dynamics by observing how plants interact with their environment. I explored how leaf nutrient concentrations were affected over successional time by environmental changes. The majority of prior research on functional traits focuses on either woody or herbaceous species, but not both life forms simultaneously. To address this, I also explored the successional changes to leaf nutrient concentrations, separating the plant community into woody and herbaceous species.

Healthy, mature leaves were taken from 122 taxa of woody and herbaceous species in a ...


Rare Occurrences Of Free-Living Bacteria Belonging To Sedimenticola From Subtidal Seagrass Beds Associated With The Lucinid Clam, Stewartia Floridana, Aaron M. Goemann Dec 2015

Rare Occurrences Of Free-Living Bacteria Belonging To Sedimenticola From Subtidal Seagrass Beds Associated With The Lucinid Clam, Stewartia Floridana, Aaron M. Goemann

Masters Theses

Lucinid clams and their sulfur-oxidizing endosymbionts comprise two compartments of a three-stage, biogeochemical relationship among the clams, seagrasses, and microbial communities in marine sediments. A population of the lucinid clam, Stewartia floridana, was sampled from a subtidal seagrass bed at Bokeelia Island Seaport in Florida to test the hypotheses: (1) S. floridana, like other lucinids, are more abundant in seagrass beds than bare sediments; (2) S. floridana gill microbiomes are dominated by one bacterial operational taxonomic unit (OTU) at a sequence similarity threshold level of 97% (a common cutoff for species level taxonomy) from 16S rRNA genes; and (3) the ...


Rnai Mediated Silencing Of Cell Wall Invertase Inhibitors To Increase Sucrose Allocation To Sink Tissues In Transgenic Camelina Sativa Engineered With A Carbon Concentrating Mechanism, Joshua Zuber Jul 2015

Rnai Mediated Silencing Of Cell Wall Invertase Inhibitors To Increase Sucrose Allocation To Sink Tissues In Transgenic Camelina Sativa Engineered With A Carbon Concentrating Mechanism, Joshua Zuber

Masters Theses

Plant invertases are a class of proteins that have enzymatic function in cleaving sucrose to fructose and glucose. Cell wall invertase, located on the exterior of the cell wall of plant cells, plays a key role in the unloading of sucrose from the apoplast to the sink tissues. Cell wall invertase interacts with an inhibitor, cell wall invertase inhibitor, post-transcriptionally to regulate its activity. The inhibitor is constitutively expressed in pollen development, early developing seeds, and senescing leaves: indicative of sucrose allocation being a limiting factor at these stages of development. We introduced algal bicarbonate transporters LCIA/CCP1 to Camelina ...


Distribution Of Abc Transporter Genes Across The Plant Kingdom, Thomas Scott Lane May 2015

Distribution Of Abc Transporter Genes Across The Plant Kingdom, Thomas Scott Lane

Masters Theses

The ATP-binding cassette (ABC) transporter gene superfamily is ubiquitous among extant organisms. ABC transporters act to transport compounds across cellular membranes and are involved in a diverse range of biological processes and functions including cancer resistance in humans, drug resistance among vertebrates, and herbicide resistance in weeds. This superfamily of genes appears to be larger and more diverse in the plant kingdom—yet, we know relatively less about ABC transporter function in plants compared with mammals and bacteria. Therefore, we undertook a plant kingdom-wide transcriptomic survey of ABC transporters to better understand their diversity.

We utilized sequence similarity-based informatics techniques ...


Thermocycle-Regulated Wall Regulator Interacting Bhlh Encodes A Protein That Interacts With Secondary-Cell-Wall-Associated Transcription Factors, Ian P. Whitney Mar 2015

Thermocycle-Regulated Wall Regulator Interacting Bhlh Encodes A Protein That Interacts With Secondary-Cell-Wall-Associated Transcription Factors, Ian P. Whitney

Masters Theses

Lignocellulosic biomass is one of the most abundant raw materials on earth that can be utilized to created carbon-neutral biofuels as a replacement for conventional fossil fuels. In order to create ideal energy crops, the regulation and deposition of cell wall polysaccharides must first be fully understood. Improved understanding of cell wall regulation will enable selection of traits that can optimize biofuel feedstocks. Herein, I utilize the grass model system Brachypodium distachyon in order to understand the transcriptional regulation of secondary cell wall deposition. Gene expression profiling was used to elucidate transcription factors that regulate secondary cell wall biosynthesis. Through ...


The Functional Role Of Leaf Nutrients In An Old-Field Successional Community, Kirstin I. Duffin Jan 2015

The Functional Role Of Leaf Nutrients In An Old-Field Successional Community, Kirstin I. Duffin

Masters Theses

In functional ecology, traits that capture aspects of plant performance are used to understand how organisms interact with their environment. Leaf nutrients are an example of a functional trait that directly links to plant metabolic processes and therefore may describe plant assemblage dynamics. Multivariate leaf nutrient analyses may be used with other functional traits to understand ecological strategies because they are a direct measure of leaf metabolic processes and can describe nuances in plant allocation patterns. In this thesis, I explored (1) whether a suite of leaf nutrients (carbon, nitrogen, phosphorus, potassium, calcium, and magnesium) was related to plant growth ...


Volatile Profiles And Resistance To Herbivory In Eastern Hemlock, Elizabeth A. Mckenzie Nov 2014

Volatile Profiles And Resistance To Herbivory In Eastern Hemlock, Elizabeth A. Mckenzie

Masters Theses

Eastern hemlock hosts the hemlock woolly adelgid, an introduced sap-feeding insect that causes rapid deterioration of the host. Like most conifers, eastern hemlock produces a variety of constitutive and induced defenses, primarily terpenoids. To explore the relationship of terpenoid defenses with adelgid infestations, we artificially infested hemlocks at a forest site and a plantation site, and compared their terpenoid concentrations to those in control trees. Infested trees showed lower terpenoid concentrations than control trees, suggesting that eastern hemlock not only fails to induce production of terpenoids in response to adelgid infestation, but becomes less able to produce carbon-based defenses due ...


Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo Nov 2014

Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo

Masters Theses

The work presented here focuses on the molecular mechanism of phytosiderophore secretion in graminaceous plants. In maize, yellow stripe3 (ys3) is a mutant that is deficient in its ability to secrete iron-chelating compounds of the mugineic acid family known as phytosiderophores. Phytosiderophores are specific to grasses and are used for the acquisition of iron. Genetic linkage mapping of the ys3 locus lead to a region of interest on chromosome 3 defined by marker UMC1773. The sequence of eleven candidate genes (GRMZM2G390345, GRMZM2G390374, GRMZM2G342821, GRMZM5G800764, GRMZM2G502560, GRMZM5G849435, GRMZM2G105766, GRMZM5G876835, GRMZM2G036976, GRMZM2G502563, miR167g) revealed several small deletions and point mutations within the ...


Evaluating Pollination Ecology Of The Endangered Pityopsis Ruthii (Small) Small (Asteraceae), Philip Anthony Moore May 2014

Evaluating Pollination Ecology Of The Endangered Pityopsis Ruthii (Small) Small (Asteraceae), Philip Anthony Moore

Masters Theses

Pityopsis ruthii (Small) Small, also known as Ruth’s golden aster, is a federally endangered herbaceous perennial, endemic to two river systems, the Hiwassee and the Ocoee, within the Cherokee National Forest, Polk County, Tennessee. There are approximately 13,000 individuals that may be at high risk of short-term extirpation (Thompson and Schwartz, 2006). Little is known of the basic reproduction and life history of P. ruthii. Clebesh and Sloan (1993), Cruzan (2001), Park (1998), and Wadl et al. (2014) found evidence that seed production and seed viability are highly variable. Clebesh and Sloan (1993) indicated that pollinator visitation was ...


Morphological And Ecological Investigations Of Species Of Bulrush (Scirpus) In Illinois, Julian G. Moore Jan 2014

Morphological And Ecological Investigations Of Species Of Bulrush (Scirpus) In Illinois, Julian G. Moore

Masters Theses

Two closely related species of bulrush, Scirpus atrovirens (Green bulrush) and Scirpus georgianus (Georgia bulrush), are widely distributed in Illinois. They are difficult to separate in the field, but readily distinguishable under magnification in the lab. These two species have been found in moist meadows, shallow marshes, edges of wet forests, and ditches. The objective of this study was to evaluate the morphological and ecological distinctness of the species with the aim toward discovering and describing other features to distinguish them reliably. Eighty plants of Scirpus atrovirens and 80 of Scirpus georgianus were examined. Fifty S. atrovirens and 50 S ...


Use Of Capacitance Sensors For Development Of Conservative Irrigation Regimes, Ethan Daniel Hagen Aug 2013

Use Of Capacitance Sensors For Development Of Conservative Irrigation Regimes, Ethan Daniel Hagen

Masters Theses

Several experiments were conducted to further develop capacitance sensor-based automated irrigation systems. The first experiment tested whether the photosynthetic response to decreasing volumetric water content (VWC) differed among four species tested. A sigmoidal curve best described the relationship for all species (r2 [r-squared]>0.86). The VWC that maintained maximum photosynthesis at 90% was selected as a potential conservative irrigation set point and values were not different between species, nor were 100% container capacity values. This indicates that a single set point is adequate to initiate irrigation and that a common upper threshold for VWC can be used for ...


Impacts Of Preemergence Herbicides On Hybrid Bermudagrass Morphology, Patrick Alan Jones May 2013

Impacts Of Preemergence Herbicides On Hybrid Bermudagrass Morphology, Patrick Alan Jones

Masters Theses

Preemergence (PRE) herbicides were evaluated in multiple experiments to determine their impacts on hybrid bermudagrass morphology [(C. dactolyn (L.) Pers. x C. transvaalensis (Burtt Davy)]. First, indaziflam and prodiamine were applied to hybrid bermudagrass rooted to various depths in either sand or silt loam mini-rhizotrons. Results indicated that prodiamine (a mitiotic inhibitor) and indaziflam (a cellulose biosynthesis inhibitor) both significantly reduced root length density compared to a non-treated control; however, indaziflam treated plants exhibited foliar injury (i.e., reddening, necrosis) while those treated with prodiamine did not.

The researchers surmised that reductions in root length density with indaziflam may compromise ...


Distribution And Growth Of Autumn Olive In A Managed Landscape, Matthew Ruddick Moore May 2013

Distribution And Growth Of Autumn Olive In A Managed Landscape, Matthew Ruddick Moore

Masters Theses

Invasions by exotic plant species result in significant challenges for forest managers. Disturbance and increased light have been shown to facilitate the successful establishment and invasion of exotic, invasive plant species. Several studies have sought to determine which key factors lead to greater abundance of exotic, invasive plants on certain sites and this information is important for determining the likelihood for exotic plant invasions at broad scales. Site characteristics that may promote autumn olive (Elaeagnus umbellata) were studied. Our goal was to identify variables associated with forest road edges most important in explaining autumn olive abundance and growth. The objectives ...


Interacting Effects Of Fire Activity, Climate, And Habitat Diversity On Forest Dynamics, El Malpais National Monument, New Mexico, Usa, Alex John Pilote May 2012

Interacting Effects Of Fire Activity, Climate, And Habitat Diversity On Forest Dynamics, El Malpais National Monument, New Mexico, Usa, Alex John Pilote

Masters Theses

The historic effects of fire and climate on stand dynamics in the ponderosa pine-dominated forests of the American Southwest are of increasing concern to land management agencies. Using present forest stand structure, the mixed-conifer forests of the volcanic features in El Malpais National Monument were analyzed at three separate sites: a cinder cone, an ancient basalt flow, and an isolated “island” (kipuka) completely surrounded by basalt flows. Increment cores were collected from 632 trees in 19 plots within the monument to obtain dates of establishment. These dates were compared with historic fire histories and precipitation records to analyze the effects ...


Control Of Glyphosate Resistant Horseweed (Conyza Canadensis) With Saflufenacil And Tank-Mixture Partners., Brock Steven Waggoner Dec 2010

Control Of Glyphosate Resistant Horseweed (Conyza Canadensis) With Saflufenacil And Tank-Mixture Partners., Brock Steven Waggoner

Masters Theses

Field and labratory studies were conducted to determine the efficacy of saflufenacil alone and with mixture partners for burndown. Field studies were conducted in 2009 and 2010 to evaluate saflufenacil in mixtures with glyphosate, glufosinate, or paraquat for control of glyphosate-resistant (GR) horseweed prior to planting cotton. Saflufenacil and saflufenacil mixtures were applied 7 days before planting (DBP). Saflufenacil at 25 and 50 g ai ha-1 in mixture with all three non-selective herbicides provided similar GR horseweed control when compared to the current standard of glyphosate plus dicamba. Control of GR horseweed was also not different at the 25 and ...


Effects Of Trinexapac-Ethyl And Winter Overseeding On The Morphological Characteristics And Traffic Tolerance Of Bermudagrass Cultivars, William D. Haselbauer May 2010

Effects Of Trinexapac-Ethyl And Winter Overseeding On The Morphological Characteristics And Traffic Tolerance Of Bermudagrass Cultivars, William D. Haselbauer

Masters Theses

Common bermudagrass [Cynodon dactylon (L.) Pers.] and hybrid bermudagrass [C. dactylon (L.) Pers. X C. transvaalensis Burtt-Davy] are some of the most common turfgrasses used on athletic fields. Subsequently, the traffic tolerance of commercially available cultivars and the effects of trinexapac-ethyl (TE) on bermudagrass athletic fields with or without perennial ryegrass are often limited. A two year study at the University of Tennessee was conducted under simulated athletic field traffic to evaluate the performance of bermudagrass cultivars as affected by TE and overseeding. Bermudagrass cultivars evaluated were Tifway, Riviera, Patriot, and Celebration. TE treatments consisted of an untreated control, TE ...


Early Vessel Evolution And The Diversification Of Wood Function: Insights From The Malagasy Canellales, Patrick Joseph Hudson May 2010

Early Vessel Evolution And The Diversification Of Wood Function: Insights From The Malagasy Canellales, Patrick Joseph Hudson

Masters Theses

Xylem vessels have long been proposed as a key innovation for the ecological diversification of angiosperms by providing a breakthrough in hydraulic efficiency to support high rates of photosynthesis and growth. However, recent studies demonstrated that angiosperm woods with structurally ‘primitive’ vessels did not have greater whole stem hydraulic capacities as compared to vesselless angiosperms. As an alternative to the hydraulic superiority hypothesis, the heteroxylly hypothesis proposes that subtle hydraulic efficiencies of primitive vessels over tracheids enabled new directions of functional specialization in the wood. However, the functional properties of early heteroxyllous wood remain unknown. We selected the two species ...


Comparative Ecophysiology Of American Chestnut Under Different Planting Treatments On Reclaimed Mine Sites, Christopher Ryan Miller May 2010

Comparative Ecophysiology Of American Chestnut Under Different Planting Treatments On Reclaimed Mine Sites, Christopher Ryan Miller

Masters Theses

American chestnut was once an abundant species that dominated the Eastern U.S. deciduous forests. Although this species is currently functionally extinct due to the chestnut blight, researchers are working on blight-resistant hybrids in hopes of restoring the species. As one potential vector for chestnut reintroduction and dispersal, the reclamation of mine sites are being considered. Recent research has found that reforestation efforts on these reclaimed mine sites provide productive tree growth while also complying with mine-reclamation laws. Understanding how American chestnut performs physiologically on mine sites will aid in the restoration of this species and reclamation of mine sites ...


Tissue Culture In Rice (Oryza Sativa L. Subsp. Japonica Var. Lemont), Young M. Woo Jan 1989

Tissue Culture In Rice (Oryza Sativa L. Subsp. Japonica Var. Lemont), Young M. Woo

Masters Theses

The purpose of this study was to obtain callus induction and subsequent plantlet regeneration from various explants of rice (Oryza sativa L. subsp. japonica var. Lemont). Callus induction occurred from the somatic cells of ovary wall, immature and mature embryos, immature endosperm, stem nodes, and seedlings. Plantlets were regenerated from embryo-, endosperm-, and stem node-derived calli on differentiation media. Stem nodes were the most responsive to callus induction. Requirements for optimal callus formation and plantlet regeneration differed from each culture. The study investigated the effects of cold treatment of explants, initial culture in darkness, media, and subculture for individual explant ...