Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 8 of 8

Full-Text Articles in Life Sciences

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal Dec 2016

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1∆11 and 18:1∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier proteins (ACPs); thioesterases (TEs) and β-ketoacyl-ACP …


Propagation And Container Production Of The Amelanchier Spicata Complex, Gregory J. Melcher Aug 2016

Propagation And Container Production Of The Amelanchier Spicata Complex, Gregory J. Melcher

Electronic Theses and Dissertations

Amelanchier is a genus in the Rosaceae containing shrubs and trees indigenous to North America that possess traits valued by the horticulture industry. Amelanchier spicata (dwarf serviceberry), a heterogeneous complex of hybrids indigenous to eastern North America, has agricultural and ornamental merit with notable characteristics. White blossoms emerge in early spring, edible pomes ripen in the summer, and vibrant, orange foliage occurs in the fall. I investigated phenological and physiological factors influencing adventitious rooting of stem cuttings, the effects of nitrogen source on the development of seedlings grown in container culture, and phenotypic variation among seedlings of A. spicata from …


Characterization Of Sip470, A Family 1 Lipid Transfer Protein And Its Role In Plant Stress Signaling, Timothy Ndagi Audam Aug 2016

Characterization Of Sip470, A Family 1 Lipid Transfer Protein And Its Role In Plant Stress Signaling, Timothy Ndagi Audam

Electronic Theses and Dissertations

SIP470, a putative tobacco lipid transfer protein, was identified in a yeast two-hybrid screen to interact with SABP2. SABP2 is a critical role in SA-mediated signaling in tobacco and other plants. In vitro studies using purified recombinant SIP470 confirmed that it is a lipid binding protein. In an attempt to determine its role in mediating stress responses, Arabidopsis T-DNA insertion knockout lines lacking SIP470 homolog were used for the analysis. These mutant plants were defective in basal resistance against microbial pathogens. Expression of defense gene PR-1 was also delayed in these mutant plants. Interestingly, these mutant plants were not defective …


Evaluation And Identification Of Soybean Aphid Resistance Sources And Mapping Of Soybean Aphid Resistance Loci In Early Maturing Soybean Germplasm Accessions, Siddhi Jeewan Bhusal Jan 2016

Evaluation And Identification Of Soybean Aphid Resistance Sources And Mapping Of Soybean Aphid Resistance Loci In Early Maturing Soybean Germplasm Accessions, Siddhi Jeewan Bhusal

Electronic Theses and Dissertations

Soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America particularly in the northern United States and three Canadian provinces. At least four biotypes of soybean aphid have been confirmed in the United States. Identification of soybean aphid resistance sources in early-maturing soybeans and genetic characterization of new sources of resistance will facilitate to expand the gene pool of soybean aphid resistance and thus will help to develop soybean aphid resistant cultivars. To identify new sources of resistance in early maturing soybeans, 330 soybean germplasm accessions from Maturity Group (MG) I, …


Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah Jan 2016

Cooperation And Punishment In The Arbuscular Mycorrhizal Symbiosis: Implications For Resource Exchange & Biological Market Dynamics, Jerry A. Mensah

Electronic Theses and Dissertations

The arbuscular mycorrhizal (AM) symbiosis is arguably the world’s most abundant and important mutualism, and brings together the roots of the majority of land plants and AM fungi to great mutual advantage. The AM symbiosis can increase the uptake of nutrients, such as phosphorus (P) and nitrogen (N), and improves the abiotic and biotic stress resistance of the host plant. AM fungi have the potential to act as biofertilizers and bioprotectors in sustainable agriculture. However, despite its significance, the mechanisms that control the resource exchange between both partners in the arbuscular mycorrhizal symbiosis are largely unknown. The main aim of …


Effects Of Seed Applied Fungicide On Arbuscular Mycorrhizal Colonization Of South Dakota Cultivars Of Oat, Soybean, And Corn, Jesse Cameron Jan 2016

Effects Of Seed Applied Fungicide On Arbuscular Mycorrhizal Colonization Of South Dakota Cultivars Of Oat, Soybean, And Corn, Jesse Cameron

Electronic Theses and Dissertations

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that form a mutualistic relationship with approximately 80% of terrestrial plant species. These obligate symbionts have a generally beneficial effect on their host such as increased nutrient acquisition, better tolerance to biotic and abiotic stresses, and the improvement of soil qualities. Due to the recent, widespread use of seed applied fungicides on row crops in the U.S. Midwest, there are concerns that the fungicides will inhibit these beneficial mycorrhizae. This study was conducted to evaluate what effect different commonly used seed applied fungicides have on AMF in the presence of different varieties of …


A Molecular Genetic And Physiological Comparison Of The Hybrid Necrosis Response In Wheat (Triticum Aestivum L.) To Biotic And Abiotic Stress Responses, Devi R. Kandel Jan 2016

A Molecular Genetic And Physiological Comparison Of The Hybrid Necrosis Response In Wheat (Triticum Aestivum L.) To Biotic And Abiotic Stress Responses, Devi R. Kandel

Electronic Theses and Dissertations

Expression of hybrid necrosis in plants can lead to a significant reduction in productivity, or even lethality. Epistatic interactions between divergent loci that have evolved through evolution are proposed as being responsible for the genetic incompatability that is expressed as hybrid necrosis. Hybrid necrosis can also represent an obstacle to the transfer of desirable traits from wild, related species to domesticated bread wheat. In wheat, expression of dominant complementary genes Ne1 and Ne2, located to chromosomes 5B and 2B in a hybrid results the production of necrotic leaf tissue, stunted plant growth, and reduced grain yield, which are similar plant …


Role Of Silencing Rna Fgsir34 In Fusarium Graminearum's Pathogenicity To Wheat, Subha Dahal Jan 2016

Role Of Silencing Rna Fgsir34 In Fusarium Graminearum's Pathogenicity To Wheat, Subha Dahal

Electronic Theses and Dissertations

Fusarium graminearum is an ascomycetous fungal pathogen that causes Fusarium head blight (FHB) disease in wheat and other cereal grains. Mycotoxin produced by the fungus, predominantly deoxynivalenol (DON), is considered as an important virulence factor for the spread of disease. Our previous study of a Dicer-like 2 knockdown mutant has led to our hypothesis that a silencing RNA, fgsiR34, might play a key role in regulating DON biosynthesis and some other virulent factors. To test this hypothesis, we generated an fgsiR34 over-expressing mutant (ΔfgsiR34+) using Inverse Repeat Transgene method and studied the pathogenicity of the mutant in …