Life Sciences Commons^™

Leica Microscope Gpu Deconvolution Stellaris Fish Dataset #1, George Mcnamara

George McNamara

McNamara 20131101Fri Leica widefield microscope CUDA Deconvolution Stellaris FISH probe cultured cells dataset #1.zip

A text file in the zip archive has experiment details. I am posting this online so that researchers - whether academic or commercial - can evaluate the acquired data, the Bruce and Butte 2013 Optics Express ( http://www.opticsinfobase.org/oe/fulltext.cfm?uri=oe-21-4-4766 ) deconvolution result (note: I may not have used optimal settings), and to compare these deconvolution results to other methods. If anyone generates alternative spatial deconvolution output, such as from: * SVI Huygens * Media Cy AutoQuant * Agard's ER-Decon (Arigovindan 2013 PNAS) * Vicidomini SGP GPU Deconv …

Supervillin Binding To Myosin Ii And Synergism With Anillin Are Required For Cytokinesis, Tara C. Smith, Peter C. Fridy, Yinyin Li, Shruti Basil, Sneha Arjun, Ryan M. Friesen, John D. Leszyk, Brian T. Chait, Michael P. Rout, Elizabeth J. Luna

Elizabeth J. Luna

Cytokinesis, the process in which cytoplasm is apportioned between dividing daughter cells, requires coordination of myosin II function, membrane trafficking and central spindle organization. Most known regulators act during late cytokinesis; a few, including the myosin II-binding proteins anillin and supervillin, act earlier. Anillin's role in scaffolding the membrane cortex with the central spindle is well established, but the mechanism of supervillin action is relatively uncharacterized. We show here that two regions within supervillin affect cell division: residues 831-1281, which bind central spindle proteins, and residues 1-170, which bind the myosin II heavy chain (MHC) and the long form of …

Prolactin And Fmri Response To Skf38393 In The Baboon, Brad D. Miller, Lauren A. Marks, Jonathan M. Koller, Blake J. Newman, G Larry Bretthorst, Kevin J. Black

Kevin J. Black, MD

Background: This study’s goal was to provide dose-response data for a dopamine agonist in the baboon using standard methods (replicate measurements at each dose, across a range of doses), as a standard against which to subsequently validate a novel pharmacological MRI (phMRI) method. Dependent variables were functional MRI (fMRI) data from brain regions selected a priori, and systemic prolactin release. Necessary first steps included estimating the magnitude and time course of prolactin response to anesthesia alone and to various doses of agonist. These first steps (“time course studies”) were performed with three agonists, and the results were used to select …

Welcome To The Journal Of Evolution And Health, Aaron Blaisdell, Paul Jaminet, David C. Pendergrass

Aaron P Blaisdell

Welcome to the first issue of the Journal of Evolution and Health! The Journal of Evolution and Health is the peer-reviewed, open-access journal of the Ancestral Health Society, a community of scientists, healthcare professionals, and laypersons who collaborate to understand health challenges from an evolutionary perspective.

Preseason And Post Season Body Composition Does Not Change Relative To Playing Time In Division I Female Basketball Players, Emma Ladwig, Andrew Shim, Jae Yom, Patrick Cross, Justin Beebe

Andrew L Shim

International Journal of Exercise Science 6(3) : 208-216, 2013. The purpose of the study was to determine if preseason and postseason body fat percentages (BF%) change relative to playing time in Division I women’s basketball players. Subjects for the study included 11 National Collegiate Athletic Association (NCAA) Division I female collegiate basketball athletes over the age of 18 from a Midwest public university. Demographic data of each subject (age 20.09 +1.81 yrs., weight 71.13 +10.85 kg., height 176.48 + 8.33 cm.) was taken before the initial body fat assessment. The subjects underwent preseason and postseason BOD POD® testing to get …

Alcohol-Induced Il-1beta In The Brain Is Mediated By Nlrp3/Asc Inflammasome Activation That Amplifies Neuroinflammation, Dora Lippai, Shashi Bala, Jan Petrasek, Timea Csak, Ivan Levin, Evelyn Kurt-Jones, Gyongyi Szabo

Gyongyi Szabo

Alcohol-induced neuroinflammation is mediated by proinflammatory cytokines, including IL-1beta. IL-1beta production requires caspase-1 activation by inflammasomes-multiprotein complexes that are assembled in response to danger signals. We hypothesized that alcohol-induced inflammasome activation contributes to increased IL-1beta in the brain. WT and TLR4-, NLRP3-, and ASC-deficient (KO) mice received an ethanol-containing or isocaloric control diet for 5 weeks, and some received the rIL-1ra, anakinra, or saline treatment. Inflammasome activation, proinflammatory cytokines, endotoxin, and HMGB1 were measured in the cerebellum. Expression of inflammasome components (NLRP1, NLRP3, ASC) and proinflammatory cytokines (TNF-alpha, MCP-1) was increased in brains of alcohol-fed compared with control mice. Increased …

A Diabetic Adolescent Case Study: Use Of A Website In Combination With An Exercise Program To Increase Physical Activity, Danielle Buckley, Nicole Demilia, Stephanie Tanzi, Furong Xu, Linda Lamont

Linda S. Lamont Ph.D.

International Journal of Exercise Science 5(3) : 296-300, 2012. The purpose of this study was to report on the efficacy of a web-based technology support to encourage physical activity in children. This program was designed to promote physical activity and proper nutrition in the diabetic adolescent with a weekly meeting that consisted of a 30-minute educational session followed by 60-minutes of exercise. A specifically designed website was used as a support to this weekly supervised exercise program. Outcomes assessment included body mass index (BMI), muscle strength (grip strength, back and leg strength), flexibility, exercise self-efficacy, and physical activity participation (pedometer, …

The Effects Of Insoles On Loading Rate In Level Running, Crystal Ruano, Douglas Powell, Doug Renshaw, Elizabeth Chalambaga, Matthew Bice

Doug Renshaw

Introduction: Running is one of the most popular recreational sports in America with over 250 million regular runners. Recreational and competitive runners often experience over-use injuries including plantar fasciitis, Achilles tendinitis and stress fractures. Previous research has shown that the transmission of ground reaction force through the lower extremity leads to these over-use injuries; however, it is not solely the magnitude of vertical ground reaction forces, but also the rate at which these forces are applied that may lead to lower extremity injury. Many recreational runners will use over-the-counter insoles as a method of treating an injury or as a …

Lower Extremity Kinetics In High And Low-Arched Athletes During Landing, Elizabeth T. Chalambaga, Douglas Powell, Doug Renshaw, Matthew Bice, Benjamin Long

Doug Renshaw

Abnormal foot function has been associated with an increased rate of injury in the athletic population. It has been shown that high-arched (HA) and low-arched (LA) athletes experience different injury patterns. These may be the manifestation of different loading and joint torque patterns in HA and LA athletes. It has been shown that HA and LA athletes have unique kinematic and kinetic patterns during running. However, little research has examined ground reaction forces (GRF) and lower extremity joint kinetics in HA and LA athletes during landing tasks. PURPOSE: To examine GRF and knee and ankle joint torques in HA and …

Refeeding After Acute Food Restriction: Differential Reduction In Preference For Ethanol And Ethanol-Paired Flavors In Selectively Bred Rats., Clinton Chapman, Nancy Dess, Laura Cousins, Phuong Nguyen

Clinton D Chapman

Rats' voluntary ethanol intake varies with dispositional factors and energy status. The joint influences of these were of interest here. We previously reported that rats selectively bred for high voluntary saccharin intake (HiS) consume more ethanol and express more robust conditioning of preference for flavors paired with voluntarily consumed ethanol than do low-saccharin consuming counterparts (LoS). Three new experiments examined the effect of refeeding after an episode of food restriction on ethanol intake and on preference for ethanol-paired flavors in HiS and LoS rats. A 48-h episode of food restriction with wheel running reduced intake of and preference for 4% …

Seawater Flow Into The Digestive System Of Actinotroch Larvae (Phoronida), William Jaeckle

William Jaeckle

Actinotroch larva (Phoronida) concentrate and collect food particles during their planktonic existance. Reported mechanisms of particle capture include both ciliary reversal and direct interception along the larval tentacles, and suction of particle-containing seawater into the larval vestibule. These diverse mechanisms explain collection of particles, but not subsequent ingestion. Particle delivery into the digestive system is accomplished, at least in part, by the activity of the esophageal cilia. If and how the beat frequency of esophageal cilia is modified by the presence or absence of particulate or dissolved organic materials remains unknown for actinotroch larvae.