Life Sciences Commons^™

Outbreak Of Epizootic Hemorrhagic Disease In Captive Reindeer (Rangifer Tarandus), Emma H. Torii, Arno Wünschmann, Mia Kim Torchetti, Leo Koster, Albert Van Geelen, Randy Atchison, Anne Rivas

USDA Wildlife Services: Staff Publications

In September 2020, an outbreak of epizootic hemorrhagic disease occurred in captive reindeer (Rangifer tarandus) and was associated with neurological signs and mortality. Four reindeer died or were euthanized after acute illness over a 12-day period. Affected reindeer displayed abnormal behavior, neurologic signs, lethargy, and/or lameness. The most consistent gross finding was dark red streaks throughout the adrenal gland cortices (4/4). One animal had acute hemorrhage involving the subcutis and skeletal muscles over the ventrolateral body wall and back, and abomasal serosa. Histologically, the most common lesions were adrenal gland cortical hemorrhage (4/4) with necrosis (3/4) and lymphoplasmacytic meningoencephalitis with …

Sequence-Based Bioinformatics Approaches To Predict Virus-Host Relationships In Archaea And Eukaryotes, Yingshan Li

Department of Computer Science and Engineering: Dissertations, Theses, and Student Research

Viral metagenomics is independent of lab culturing and capable of investigating viromes of virtually any given environmental niches. While numerous sequences of viral genomes have been assembled from metagenomic studies over the past years, the natural hosts for the majority of these viral contigs have not been determined. Different computational approaches have been developed to predict hosts of bacteria phages. Nevertheless, little progress has been made in the virus-host prediction, especially for viruses that infect eukaryotes and archaea. In this study, by analyzing all documented viruses with known eukaryotic and archaeal hosts, we assessed the predictive power of four computational …

(R1980) Effect Of Climate Change On Brain Tumor, Pardeep Kumar, Sarita Jha, Rajiv Aggarwal, Govind Kumar Jha

Applications and Applied Mathematics: An International Journal (AAM)

In this paper, we introduce a new dynamical model addressing the variation in climate condition due the presence of microorganisms. We also introduce a new dynamical model of cancer growth which includes three interactive cell populations with drug free environment, namely tumor cells, healthy host cells, and immune effector cells. In this, we considered the super growth of tumor cells. For the choice of certain parameters, both of the systems exhibit chaotic behavior. The aim of this work is to design the controller to control the chaos and to provide sufficient conditions which achieve synchronization of two non-identical systems, which …

Where To Invest Project Efforts For Greater Benefit: A Framework Formanagement Performance Mapping With Examples For Potato Seed Health, C. E. Buddenhagen, Y. Xing, J. L. Andrade-Piedra, G. A. Forbes, P. Kromann, I. Navarrete, S. Thomas-Sharma, Robin A. Choudhury, K. F. Andersen Onofre, E. Schulte-Geldermann

School of Earth, Environmental, and Marine Sciences Faculty Publications and Presentations

Policymakers and donors often need to identify the locations where technologies are most likely to have important effects, to increase the benefits from agricultural development or extension efforts. Higher-quality information may help to target the high-benefit locations, but often actions are needed with limited information. The value of information (VOI) in this context is formalized by evaluating the results of decision making guided by a set of specific information compared with the results of acting without considering that information. We present a framework for management performance mapping that includes evaluating the VOI for decision making about geographic priorities in regional …

Contributions Of Vibrational Spectroscopy To Virology: A Review, Iqra Chaudhary, Naomi Jackson, Denise Denning, Luke O'Neill, Hugh Byrne

Articles

Vibrational spectroscopic techniques, both infrared absorption and Raman scattering, are high precision, label free analytical techniques which have found applications in fields as diverse as analytical chemistry, pharmacology, forensics and archeometrics and, in recent times, have attracted increasing attention for biomedical applications. As analytical techniques, they have been applied to the characterisation of viruses as early as the 1970s, and, in the context of the coronavirus disease 2019 (COVID-19) pandemic, have been explored in response to the World Health Organisation as novel methodologies to aid in the global efforts to implement and improve rapid screening of viral infection. This review …

Understanding Of Aerosol Transmission Of Covid 19 In Indoor Environments, Adama Barro, Cathal O'Toole, Jacob S. Lopez, Matthew Quinones, Sherene Moore

Publications and Research

Our reason for discussing severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) or 2019 novel corona virus (Covid-19), is to understand its aerosol transmission characteristics in indoor spaces and to mitigate further spread of this disease by designing a new HVAC system. The problem that we are tackling is the spread of covid-19 droplets through aerosol transmission by looking at potential engineering solutions to the existing HVAC systems. The purpose is to eradicate the spread of the COVID-19 by testing indoor spaces in an effort to understand the effectiveness of ventilation controls. We believe that scientists and engineers have not …

Innovative Approaches In The Discovery Of Aquatic Mycobacteriophages, Janis H. Doss

Biomedical Sciences Theses & Dissertations

Bacteriophages (phages), viruses that infect bacteria, have many applications in medicine, agriculture, molecular biology, and other fields. As antibiotic resistance becomes an increasing problem, interest in phages has grown. The traditional techniques of phage discovery are successful for some phages, but others require modified procedures to achieve detectable host infection.

Mycobacterium is a diverse bacterial genus characterized by a unique cell wall containing mycolic acids, which aids in survival and pathogenesis. The aims of the present research were to isolate mycobacteriophages, use bioinformatics techniques to analyze mycobacterial prophages, and combine genetic analysis with multi-well plate host range studies to identify …

How We’Ll Fight The Next Deadly Virus Ted Talk Annotated Resource List, Susie Roy

Undergraduate Research Award

No abstract provided.

An Epidemiological Model Of Rift Valley Fever With Spatial Dynamics, Tianchan Niu, Holly D. Gaff, Yiannis E. Papelis, David M. Hartley

Biological Sciences Faculty Publications

As a category A agent in the Center for Disease Control bioterrorism list, Rift Valley fever (RVF) is considered a major threat to the United States (USA). Should the pathogen be intentionally or unintentionally introduced to the continental USA, there is tremendous potential for economic damages due to loss of livestock, trade restrictions, and subsequent food supply chain disruptions. We have incorporated the effects of space into a mathematical model of RVF in order to study the dynamics of the pathogen spread as affected by the movement of humans, livestock, and mosquitoes. The model accounts for the horizontal transmission of …

Amid The Vipers: Establishing Malware's Position Within The Information Ecosystem, Shawn Louis Everett Robertson

Computer Science and Software Engineering

The paper consists of a detailed examination of malware broken down into three main sections.

1. Introduction: Malware in the World Today. Begins with a definition of terms, examination of the types of malware, research into historical pieces of malicious code, a detailed analysis of the attackers, why malware is so prevalent, and why it is so hard to defend against. This section finishes with a comparison of reasons to create and not to create malware.
2. Background: "Good" Pieces of Malware. Examination of what makes malware effective. Analysis of the existing CVSS standard and proposal of the alternative VIPERS classification system. …

Discovery Of A Non-Peptidic Inhibitor Of West Nile Virus Ns3 Protease By High-Throughput Docking, Dariusz Ekonomiuk, Xun-Cheng Su, Kiyoshi Ozawa, Chrisophe Bodenreider, Siew Pheng Lim, Zheng Yin, Thomas H. Keller, David Beer, Viral Patel, Gottfried Otting, Amedeo Calflisch, Danzhi Huang

Faculty of Science - Papers (Archive)

No abstract provided.

Nmr Study Of Complexes Between Low Molecular Mass Inhibitors And The West Nile Virus Ns2b-Ns3 Protease, X Su, Kiyoshi Ozawa, Hiromasa Yagi, S P. Lim, D Wen, D Ekonomiuk, D Huang, T Keller, S Sonntag, A Caflisch, S G. Vasudevan, G Otting

Faculty of Science - Papers (Archive)

The two-component NS2B-NS3 protease of West Nile virus is essential for its replication and presents an attractive target for drug development. Here, we describe protocols for the high-yield expression of stable isotope-labelled samples in vivo and in vitro. We also describe the use of NMR spectroscopy to determine the binding mode of new low molecular mass inhibitors of the West Nile virus NS2B-NS3 protease which were discovered using high-throughput in vitro screening. Binding to the substrate-binding sites S1 and S3 is confirmed by intermolecular NOEs and comparison with the binding mode of a previously identified low molecular mass inhibitor. Our …

A Fluorescence Quenching Assay To Discriminate Between Specific And Non-Specific Inhibitors Of Dengue Virus Protease, C Bodenreider, D Beer, T Keller, S Sonntag, D Wen, Li Yap, Y H Yau, S G Shocat, D Huang, T Zhou, A Caflisch, X C Su, Kiyoshi Ozawa, G Otting, S G. Vasudevan, J Lescar, S P. Lim

Faculty of Science - Papers (Archive)

In drug discovery, the occurrence of false positives is a major hurdle in the search for lead compounds that can be developed into drugs. A small-molecular-weight compound that inhibits dengue virus protease at low micromolar levels was identified in a screening campaign. Binding to the enzyme was confirmed by isothermal titration calorimetry (ITC) and nuclear magnetic resonance (NMR). However, a structure-activity relationship study that ensued did not yield more potent leads. To further characterize the parental compound and its analogues, we developed a high-speed, low-cost, quantitative fluorescence quenching assay. We observed that specific analogues quenched dengue protease fluorescence and showed …

Nmr Analysis Of The Dynamic Exchange Of The Ns2b Cofactor Between Open And Closed Conformations Of The West Nile Virus Ns2b-Ns3 Protease, X Su, Kiyoshi Ozawa, R Qi, S G. Vasudevan, S P. Lim, G Otting

Faculty of Science - Papers (Archive)

Background: The two-component NS2B-NS3 proteases of West Nile and dengue viruses are essential for viral replication and established targets for drug development. In all crystal structures of the proteases to date, the NS2B cofactor is located far from the substrate binding site (open conformation) in the absence of inhibitor and lining the substrate binding site (closed conformation) in the presence of an inhibitor. Methods: In this work, nuclear magnetic resonance (NMR) spectroscopy of isotope and spin-labeled samples of the West Nile virus protease was used to investigate the occurrence of equilibria between open and closed conformations in solution. Findings: In …

Crop Updates - 2003 Lupins, Mark Sweetingham, Bevan J. Buirchell, Geoff Thomas, Amelia Mclarty, Harmohinder Dhammu, Martin Harries, Wayne Parker, Terry Piper, David Nicholson, Jairo Palta, Neil C. Turner, Bob French, Kedar Adhikari, Katia Stefanova, Françoise Berlandier, Glen Riethmuller, Paul Blackwell, Geoff Fosbery, Bill Crabtree, Tracy Gilham, Laurie Wahlsten, Pierre Fievez, Mike Collins, John Holmes, Dirranie Kirby, Ken Adcock, Roger Jones, Rohan Prince, Stuart Johnson, Ramon Hall, Madeleine Ball, Sofia Sipas, David Petterson

Crop Updates

This session covers twenty one papers from different authors

LUPIN ISSUES AND R & D DIRECTIONS

1. Mark Sweetingham, Department of Agriculture

   ACKNOWLEDGEMENTS

   VARIETIES AND BREEDING

2. New lupin line for release – WALAN2141, Bevan J, Buirchell, Mark Sweetingham, Geoff Thomas, Amelia McLarty, Harmohinder Dhammu and CVT and Lupin Breeding teams, Department of Agriculture

3. Lupin variety trial, Martin Harries and Wayne Parker, Department of Agriculture

4. Herbicide tolerance of new lupins, Harmohinder S. Dhammu, Terry Piper and David Nicholson, Department of Agriculture

   YELLOW AND ALBUS LUPINS

5. Selection for high lupin yield under terminal drought, Jairo A. Palta^1&2, Neil …

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Crop Updates 2002 - Farming Systems, Peter Metcalf, Mike Ewing, Roy Latta, Keith Devenish, Diana Fedorenko, Clayton Butterly, Chantelle Butterly, Kim Diamond, Neil Diamond, Stuart Mcalpine, Bill Bowden, Jessica Johns, Terry Piper, David Bowran, Robert Beard, Phil Ward, Dominie Wright, Nichole Burges, Roger Jones, Danae Harman, Greg Shea, George Yan, David Tennant, David Hall, Anyou Liu, Clinton Revell, Candy Hudson, Andrew Blake, Natalie Lauritsen, John Fosu-Nyarko, Roger Jones, Lisa Smith, Mike Jones, Geoff Dwyer, Bill Bowden, Michael O'Connell, Chris Gazey, David Gartner, Amanda Miller, Richard W. Bell, K. Frost, Mike Wong, Ross Brennan, N. J. Blake, G. Mconnell, D. Patabendige, N. Venn, Derk Bakker, Greg Hamilton, Dave Houlbrooke, Cliff Spann, Paul Blackwell, Bindi Webb, G. Lyle, K. Wittwer, Perry Dolling, Senthold Asseng, Ian Fillery, Michael Robertson, Caroline Peek, David Rogers, Peter Portman, Jeff Russell, Greg Shea, Ben Henderson, Ross Kingwell, Tresslyn Walmsley, Jean Galloway, Debbie Thackray, Moin Salam, Art Diggle, William J. Macleod, Jenny Hawkes

Crop Updates

This session covers forty one papers from different authors:

INTRODUCTION

1. Future Farming Systems session for Crop Updates 2002 Peter Metcalf, FARMING SYSTEMS SUBPROGRAM MANAGER GRAINS PROGRAM Department of Agriculture

2. Perennial pastures in annual cropping systems: Lucerne and beyond, the ‘Big Picture’, Mike Ewing, Deputy CEO CRC for Plant-based Management of Dryland Salinity, Department of Agriculture

3. Perennial pastures in annual cropping systems: lucerne and beyond, Roy Latta and Keith Devenish, Department of Agriculture

4. Establishing Lucerne with a cover crop, Diana Fedorenko¹, Clayton Butterly¹, Chantelle Butterly¹, Kim and Neil Diamond^{2 …}

Microbial Ecology Of Ballast Water During A Transoceanic Voyage And The Effects Of Open-Ocean Exchange, Lisa A. Drake, Gregory M. Ruiz, Bella S. Galil, Timothy L. Mullady, Daniela O. Friedmann, Fred C. Dobbs

OES Faculty Publications

The only procedure used frequently to reduce the risk of invasion by ballast-mediated biota is open-ocean exchange of ballast water, a procedure in which vessels release coastal water and replace it with oceanic water. Limited information exists concerning the effects of transport upon the aquatic microbial community throughout transit and following open-ocean exchange, A transoceanic voyage aboard a commercial bulk carrier afforded us the opportunity to sample the microbial community in exchanged and unexchanged ballast-water holds during the journey from Hadera, Israel to Baltimore, USA. Five days following the exchange process, all microbial metrics tested (i.e. bacteria concentration, virus-like particle …

Crop Updates 2000 - Oilseeds, Paul Carmody, Ping Si, Rodney Mailer, Nick Galwey, David Turner, Kevin Morthorpe, Stephen Addenbrooke, Peter Nelson, Mike Jackson, Scott Paton, Ross Brennan, Noeleen Edwards, Mike Bolland, Bill Bowden, C. P. Gunasekera, L. D. Martin, G. H. Walton, K. H. M. Siddique, Sharon R. Niknam, David W. Turner, Abul Hashem, Harmonhinder Dhammu, David Bowran, The Canola Association Of Western Australia, Syed H. Zaheer, Ravjit Khangura, Martin J. Barbetti, Peter Carlton, Debbie Thackray, Jenny Hawkes, Roger Jones, Brenda Coutts, Françoise Berlandier, Ross Kingwell, Michael O'Connell, Simone Bennerhasset

Crop Updates

This session covers seventeen papers from different authors:

Introduction, Paul Carmody, Centre for Cropping Systems

CANOLA AGRONOMY

2. Genotype, location and year influence the quality of canola grown across southern Australia, PingSi¹, Rodney Mailer², Nick Galwey¹ and David Turner^{1, 1}Plant Sciences, Faculty of Agriculture, The University of Western Australia, ²Agricultural Research Institute, New South Wales Agriculture

3. Development of Pioneer^® Canola varieties for Australian market,Kevin Morthorpe, StephenAddenbrooke, Pioneer Hi-Bred Australia Pty Ltd

4. Canola, Erucic Acid, Markets and Agronomic Implications, Peter Nelson, The Grain Pool …