Life Sciences Commons^™

A Study Of Naturally Occurring Algicides Produced By Freshwater Algae, Denny O. Harris, Harry D. Caldwell

KWRRI Research Reports

The mode of action of the algicide produced by Pandorina morum was examined by exposing Volvox globator and isolates spinach chloroplasts to a partially purified algicide preparation. Oxygen evolution of Volvox, whole chloroplasts and broken chloroplasts (minus the Calvin cycle),was reduced indicating that the algicide inhibits the light reactions of photosynthesis. Oxygen evolution studies of other Volvocaceae confirmed the observation that Pandorina morum is not significantly influenced by its own inhibitor. Molecular weight approximation by gel filtration established that the inhibitor has a low molecular weight (probably below 100 mw). Field studies indicate that this algicide has tremendous potential as …

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

Seasonal Changes In Water Quality And Primary Productivity In Doe Valley Lake, Edmond J. Bacon, Stuart E. Neff

KWRRI Research Reports

Primary productivity and water quality were studied in Doe Valley Lake, a 147-hectare impoundment on Doe Run, a spring-fed stream in Meade County, Kentucky, from 13 June 1969 to 31 July 1972. Doe Valley Lake is monomictic during most winter seasons, but it is dimictic during more severe winters because of its morphometry and location on the borderline climatic region for dimictic lakes (37° N latitude). Oxygen depletion in the hypolimnion is severe, and anaerobic conditions usually prevail by late June. A hypolimnetic areal deficit of 0.038 mg/cm²/day was calculated. Supersaturation of oxygen in the epilimnion was common, …

Development Of Prediction Relationships For Water Requirements With Irrigation Cooling, Billy J. Barfield, John N. Walker, F. A. Payne

KWRRI Research Reports

A model for predicting leaf temperatures during an off-on mist cycle is presented. The model uses a combination of energy budget and aerodynamic techniques. The model was tested for dry leaf temperature and evapotranspiration predictions using average hourly data from Arizona. The accuracy was good. It was also tested for dry leaf temperature predictions using two to three minute data with a widely varying net radiation. When reasonable values of stomatal resistance were used, the agreement was again good.

The model was tested for prediction of wet leaf temperature prediction with a ten minute on, fifty minute off mist irrigation …

A Study Of Water-Soluble Inhibitory Compounds (Algicides) Produced By Fresh-Water Algae, Denny O. Harris, Manhar C. Parekh

KWRRI Research Reports

A complex system of growth inhibitors was observed in the green algae (Volvocaceae). Inhibitors were found in the culture filtrates of some genera which limit their own growth (autoinhibitors) while others in the family produce substances which check the growth of other genera (heteroinhibitors). These inhibitors were destroyed by autoclaving. It was decided that Pandorina morum, which produced the strongest inhibitor and Volvox tertius, the most sensitive to the inhibitor would make an excellent model system for a study of the chemical and physical properties of these naturally occurring algicides. The algicide could be removed from actively growing cultures about …

A Detailed Investigation Of The Sociological, Economic, And Ecological Aspects Of Proposed Reservoir Sites In The Salt River Basin Of Kentucky, Stuart E. Neff, Louis A. Krumholz, John R. Baker, Daryl E. Jennings, Andrew C. Miller, Jerry S. Parsons, Vincent H. Resh, David S. White

KWRRI Research Reports

Samples of water, bottom fauna, and fishes were collected from 66 stations in the Salt River and one of its principal tributaries, the Beech Fork and its tributary, the Chaplin River, Kentucky. Precipitation ranged from 38.86 inches (1969) to 58.04 inches (1970), an increase of nearly 50 percent with marked fluctuations in discharge. Intensive comparisons of phosphates, sulfates, specific conductance, total alkalinity, total hardness, and turbidity showed the streams to be relatively clean and healthy. Nearly 300 different kinds of benthic organisms and other macroinvertebrates have been collected and identified from the basin. Detailed studies of caddisflies and stream drift …

Enzyme And Tissue Alterations In Fishes: A Measure Of Water Quality, David E. Hinton, M. W. Kendall, J. C. Koenig

KWRRI Research Reports

A variety of freshwater fishes were studied by light and electron microscopy, enzyme histochemical and biochemical methods, The objective was to determine normal structure and function in specific target organs and to compare these to altered states in aquatic pollution. The basic question, "can fish tissues and enzymes serve as indicators of water quality?," was asked. Microscopic alteration in gill was indicative of copper toxicity at an exposure of 20 parts per billion, Gross and light microscopic alterations were indicative of a single exposure of channel catfish to 15 parts per million of methyl mercuric chloride (CH₃HgCl). Microscopic …

Sensitivity Of Vertebrate Embryos To Heavy Metals As A Criterion Of Water Quality, Wesley J. Birge, John J. Just

KWRRI Research Reports

Embryonic and/or larval stages of the leopard frog (Rana pipiens), domestic fowl (Gallus domesticus) and the goldfish (Carassiua auratus) were treated with cadmium chloride, mercuric chloride, lead chloride, zinc chloride and sodium arsenite. The principal objectives were (1) to determine the sensitivity of vertebrate embryos to certain metals which are of consequence in water pollution, and (2) to ascertain the suitalility of vertebrate embryos as bioassay organisms for monitoring metallic pollutants within water resources. Vertebrate embryos are found to be highly sensitive to the toxic effects of all the metals studied. Concentrations of mercury as low as 10 ppb, with …

A Perspective On Economic Impact, L. Douglas James, Donald M. Soule, William O. Thompson, John L. Fulmer, John C. Redman, Robert C. Tussey, John M. Higgins, Claude M. Vaughan, David H. Rosenbaum, Billy R. Prebble, Charles O. Dowell, John E. Sirles, Michael B. Hargrove, Clyde T. Bates, Kenneth G. Holbrook, Dennis H. Bianchi, John P. Breaden, Kenneth R. Harman

KWRRI Research Reports

The institutions responsible for water resources management in the United States have originated as political responses to major social issues. Each agency institutionalized a procedure for structuring and comparing alternatives in the formulation of its total program. Each agency originally sought to promote effective resolution of its social issue (flood control, development of arid lands, soil erosion, etc.), but more recent efforts have sought better coordination among agency practices through a common procedure largely derived from economic theory. Any procedure, however, varies in application with the interpretation and judgment of individual planners. Today, public pressures have brought political directives requiring …

Degradation Of Riparian Leaves And The Recycling Of Nutrients In A Stream Ecosystem, Louis A. Krumholz, Roger G. Lambert, Charles R. Liston, Harry H. Woodward

KWRRI Research Reports

Leaves collected at 4 stations in the upper 5 km of Doe Run, Meade County, Kentucky, indicated an annual accumulation within the stream of 354 g/m²/year (17,700 kg). Leaves of sycamore (23.6%), red oak (21.7%), sugar maple (9.7%), beech (9.6%), white oak (7.1%), and hickory (6.0%) trees were most abundant, and leaves from 14 other kinds made up the remaining 22.3%. About a third of the annual leaf fall occurred during the last half of October and about two-thirds in the last 3 months of the year.

Calorific equivalents for different kinds of leaves ranged from 3,789 cal/g …

Factors Regulating The Growth Of Algae In Continuous Culture In Diluted Secondary Sewage Treatment Plant Effluent And Subsequent Biodegradability, Edward G. Foree, Caroline P. Wade

KWRRI Research Reports

Heterogeneous algal cultures were grown in laboratory continuous culture in continuous flow, completely mixed chemostats in secondary sewage treatment plant effluent diluted to give an ammonia nitrogen concentration of 10 mg/1. Variables were lighting, pH, carbon dioxide availability, and hydraulic residence time.

Optimum growth occurred under pH 7.0, excess CO₂, and continuous lighting conditions. The availability of artificially supplied excess CO₂ greatly increased the mass (standing crop) at steady-state over that produced under otherwise identical conditions for all residence times studied. For the case of excess CO₂ availability, the nitrogen concentration in the algal cells regulated …

A Preliminary Ecological Study Of Areas To Be Impounded In The Salt River Basin Of Kentucky, Louis A. Krumholz, Stuart E. Neff, Edmond J. Bacon, Jerry S. Parsons, John D. Woodling

KWRRI Research Reports

This report includes work that is an extension of Project No. B-005-KY as reported in Research Report No. 43 of the University of Kentucky Water Resources Institute. That project was initiated in April 1968 as Project No. A-019-KY with principal emphasis on physical, chemical, and biological characteristics of the main stem of the Salt River upstream from the proposed damsite for Taylorsville Lake, an impoundment of about 3, 600 acres at seasonal pool. The report includes descriptions of an additional 13 stations along the stream, bringing to 38 the number of permanent collecting sites.

Values for dissolved oxygen ranged from …

A Preliminary Ecological Study Of Areas To Be Impounded In The Salt River Basin Of Kentucky, Louis A. Krumholz

KWRRI Research Reports

This report covers work that is an extension of Project No. A-019-KY. A series of 25 sampling stations was established in the mainstream and tributaries of the Salt River that extend from the source of the stream in Boyle County to a few miles below the site of Taylorsville Darn in Spencer County. Sampling for water chemistry and biota was carried out semimonthly. Data on temperature, oxygen, depth, and discharge, along with analyses for cations (Ca, Mg, Fe, Mn) and anions (PO₄, NO₃, NO₂, CO₃, HCO₃) have been accumulated and …

Algal Growth And Decomposition: Effects On Water Quality, Edward G. Foree, John S. Tapp Jr.

KWRRI Research Reports

The chemical composition of algae grown in batch culture depends mainly on environmental conditions, nutrient availability, presence of predators, cell age, and species. The effects of nutrient availability and cell age on the composition of three unialgal cultures (algae + bacteria) and one hetergeneous culture (algae + bacteria + microscopic animals) were evaluated. The cultures were grown in batch culture under both nutrient-abundant and nutrient deficient conditions and the changes in compositions were observed. Luxurious uptake where nutrients are incorporated into cellular protoplasm at levels greater than those necessary for growth, and super-luxurious uptake, where some nutrients are stored rather …

Part I - Controlling The Soil Moisture Environment Of Transpiring Plants, Part Ii - Prediction Of Leaf Temperature Under Natural Atmospheric Conditions, Charles T. Haan, Billy J. Barfield, Robert Edling

KWRRI Research Reports

Part I

A technique for controlling the soil moisture potential in the root zone of transpiring plants was developed. The method uses the principles of unsaturated flow through a porous media to develop the desired moisture potential. In the case of non-steady state transpiration, the maximum possible fluctuation in the soil moisture potential can be determined by the techniques presented.

Part II

Two implicit leaf temperature prediction equations were derived from the energy balance approach. The equations define sensible and latent heat transfer from a plant population as a two step process:

1. Transfer between the plant leaf and the canopy …

The Effect Of A Large Reservoir On Local Government Revenue And Expenditure, Clyde T. Bates

KWRRI Research Reports

Development of a large multi-purpose reservoir within the area of their jurisdiction may affect property ta.x revenue and expenditure of county governments and school districts. Privately owned land sold to a federally sponsored reservoir is not subject to property taxes because of the doctrine of intergovernmental immunity. Local officials often assume that this loss of assessment will reduce their tax revenue and thereby their fiscal ability to provide an acceptable level of government services. They may also expect the influx of construction workers or the disruption of existing facilities to increase the cost of providing these services. The study approaches …

Ecological Study Of The Effects Of Strip Mining On The Microbiology Of Streams, Ralph H. Weaver, Harry D. Nash

KWRRI Research Reports

The microflora of Cane Branch of Beaver Creek in McCreary County, Kentucky, which drains an area that was strip-mined between 1955 and 1959, was studied and compared with that of Helton Branch which drains a comparable area where there has been no mining. Differences include: the establishment of Ferrcbacillus ferrooxidans, for which procedures were developed for direct colony isolation from the stream; fewer saprophytic bacteria; more numerous and more diversified filamentous and unicellular fungi; and characteristic differences in algal flora. Representatives of 42 genera of filamentous fungi were identified. Of these, 21 were isolated only from Cane Branch. Representatives of …